Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    We will mail you new results for this query: keywords==Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
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Development and validation of a maleimide-based enzyme-linked immunosorbent assay for the detection of tetrodotoxin in oysters and mussels
Reverté, Laia ; Rambla-Alegre, Maria ; Leonardo, Sandra ; Bellés, Carlos ; Campbell, Katrina ; Elliott, Christopher T. ; Gerssen, Arjen ; Klijnstra, Mirjam D. ; Diogène, Jorge ; Campàs, Mònica - \ 2018
Talanta 176 (2018). - ISSN 0039-9140 - p. 659 - 666.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) - Maleimide-based enzyme-linked immunosorbent assay (mELISA) - Mussel - Oyster - Solid-phase extraction (SPE) clean-up - Tetrodotoxin (TTX)

The recent detection of tetrodotoxins (TTXs) in puffer fish and shellfish in Europe highlights the necessity to monitor the levels of TTXs in seafood by rapid, specific, sensitive and reliable methods in order to protect human consumers. A previous immunoassay for TTX detection in puffer fish, based on the use of self-assembled monolayers (SAMs) for the immobilization of TTX on maleimide plates (mELISA), has been modified and adapted to the analysis of oyster and mussel samples. Changing dithiol for cysteamine-based SAMs enabled reductions in the assay time and cost, while maintaining the sensitivity of the assay. The mELISA showed high selectivity for TTX since the antibody did not cross-react with co-occurring paralytic shellfish poisoning (PSP) toxins and no interferences were observed from arginine (Arg). Moreover, TTX-coated maleimide plates stored for 3 months at −20 °C and 4 °C were stable, thus when pre-prepared, the time to perform the assay is reduced. When analyzing shellfish samples, matrix effects and toxin recovery values strongly depended on the shellfish type and the sample treatment. Blank oyster extracts could be directly analyzed without solid-phase extraction (SPE) clean-up, whereas blank mussel extracts showed strong matrix effects and SPE and subsequent solvent evaporation were required for removal. However, the SPE clean-up and evaporation resulted in toxin loss. Toxin recovery values were taken as correction factors (CFs) and were applied to the quantification of TTX contents in the analysis of naturally-contaminated shellfish samples by mELISA. The lowest effective limits of detection (eLODs) were about 20 and 50 µg/kg for oyster extracts without and with SPE clean-up, respectively, and about 30 µg/kg for mussel extracts with both protocols, all of them substantially below the eLOD attained in the previous mELISA for puffer fish (230 µg/kg). Analysis of naturally-contaminated samples by mELISA and comparison with LC-MS/MS quantifications demonstrated the viability of the approach. This mELISA is a selective and sensitive tool for the rapid detection of TTX in oyster and mussel samples showing promise to be implemented in routine monitoring programs to protect human health.

A collaborative evaluation of LC-MS/MS based methods for BMAA analysis : Soluble bound BMAA found to be an important fraction
Faassen, Els ; Antoniou, Maria G. ; Beekman-Lukassen, Wendy ; Blahova, Lucie ; Chernova, Ekaterina ; Christophoridis, Christophoros ; Combes, Audrey ; Edwards, Christine ; Fastner, Jutta ; Harmsen, Joop ; Hiskia, Anastasia ; Ilag, Leopold L. ; Kaloudis, Triantafyllos ; Lopicic, Srdjan ; Lurling, Miguel ; Mazur-Marzec, Hanna ; Meriluoto, Jussi ; Porojan, Cristina ; Viner-Mozzini, Yehudit ; Zguna, Nadezda - \ 2016
Marine Drugs 14 (2016)3. - ISSN 1660-3397
6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) - Cycad - Daphnia magna - Hydrophilic interaction liquid chromatography (HILIC) - Internal standard - Liquid chromatography-tandem mass spectrometry (LC-MS/MS) - N-(2-aminoethyl) glycine (AEG) - Phytoplankton - Seafood - α,γ-diaminobutyric acid (DAB) - β-N-methylamino-L-alanine (BMAA)

Exposure to β-N-methylamino-L-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D3BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D3BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (

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