- A.R.W. Elbers (1)
- Fernando Esteves (1)
- J. Godfroid (1)
- P.D. Kirkland (1)
- S. Klevar (1)
- Ahmet Kürsat Azkur (1)
- Coletha Mathew (1)
- R.H. Mdegela (1)
- G. Mwamengele (1)
- Züleyha Pestil (1)
- W.H.M. Poel van der (2)
- W.H.M. Poel Van Der (1)
- João R. Mesquita (1)
- Maria S.J. Nascimento (1)
- M. Stokstad (1)
- Sükrü Tonbak (1)
- Helena Vala (1)
Serological Evidence for Schmallenberg Virus Infection in Sheep of Portugal 2014
Esteves, Fernando ; Mesquita, João R. ; Vala, Helena ; Abreu-Silva, Joana ; Poel, W.H.M. Van Der; Nascimento, Maria S.J. - \ 2016
Vector-Borne and Zoonotic Diseases 16 (2016)1. - ISSN 1530-3667 - p. 63 - 65.
Arbovirus - ELISA. - Schmallenberg virus - Seroprevalence - Sheep
Between November and December of 2014, a serosurvey was set up to evaluate the presence of Schmallenberg virus (SBV) antibodies in sheep of Portugal. Sera (n = 1068) were tested using an indirect enzyme linked immunosorbent assay (ID Screen® Schmallenberg virus indirect, IDvet Innovative Diagnostics Montpellier, France). The estimated occurrence of immunogobulin G (IgG) antibodies against SBV in sheep of Portugal was 12.8% (95% confidence interval 11.0-15.0%). This is the first study reporting the presence of SBV antibodies in sheep of Portugal.
Circulation of Schmallenberg virus in Turkey, 2013
Tonbak, Sükrü ; Azkur, Ahmet Kürsat ; Pestil, Züleyha ; Biyikli, Emel ; Abayli, Hasan ; Baydar, Ersoy ; Poel, W.H.M. van der; Bulut, Hakan - \ 2016
Turkish Journal of Veterinary and Animal Sciences 40 (2016)2. - ISSN 1300-0128 - p. 175 - 180.
Circulation - RT-PCR - Schmallenberg virus - Turkey
Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.
Detection of serum neutralizing antibodies to Simbu sero-group viruses in cattle in Tanzania
Mathew, Coletha ; Klevar, S. ; Elbers, A.R.W. ; Poel, W.H.M. van der; Kirkland, P.D. ; Godfroid, J. ; Mdegela, R.H. ; Mwamengele, G. ; Stokstad, M. - \ 2015
BMC Veterinary Research 11 (2015). - ISSN 1746-6148 - 9 p.
Antibody ELISA - Orthobunyavirus - Schmallenberg virus - Serology - Virus neutralizing test
Background: Orthobunyaviruses belonging to the Simbu sero-group occur worldwide, including the newly recognized Schmallenberg virus (SBV) in Europe. These viruses cause congenital malformations and reproductive losses in ruminants. Information on the presence of these viruses in Africa is scarce and the origin of SBV is unknown. The aim of this study was to investigate the presence of antibodies against SBV and closely related viruses in cattle in Tanzania, and their possible association with reproductive disorders. Results: In a cross-sectional study, serum from 659 cattle from 202 herds collected in 2012/2013 were analyzed using a commercial kit for SBV ELISA, and 61 % were positive. Univariable logistic regression revealed significant association between ELISA seropositivity and reproductive disorders (OR = 1.9). Sera from the same area collected in 2008/2009, before the SBV epidemic in Europe, were also tested and 71 (54.6 %) of 130 were positive. To interpret the ELISA results, SBV virus neutralization test (VNT) was performed on 110 sera collected in 2012/2013, of which 51 % were positive. Of 71 sera from 2008/2009, 21 % were positive. To investigate potential cross reactivity with related viruses, 45 sera from 2012/2013 that were positive in SBV ELISA were analyzed in VNTs for Aino, Akabane, Douglas, Peaton, Sabo, SBV, Sathuperi, Shamonda, Simbu and Tinaroo viruses. All 45 sera were positive for one or more of these viruses. Twenty-nine sera (64.4 %) were positive for SBV, and one had the highest titer for this virus. Conclusions: This is the first indication that Aino, Akabane, Douglas, Peaton, Sabo, SBV, Sathuperi, Shamonda and Tinaroo viruses circulate and cause negative effect on reproductive performance in cattle in Tanzania. SBV or a closely related virus was present before the European epidemic. However, potential cross reactivity complicates the interpretation of serological studies in areas where several related viruses may circulate. Virus isolation and molecular characterization in cattle and/or vectors is recommended to further identify the viruses circulating in this region. However, isolation in cattle is difficult due to short viremic period of 2 to 6 days, and isolation in vectors does not necessarily reflect the situation in cattle.