Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

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GISH analyses of backcross progenies of two Lilium species hybrids and their relevance to breeding
Luo, J.R. ; Ramanna, M.S. ; Arens, P. ; Niu, L.X. ; Tuyl, J.M. van - \ 2012
Journal of Horticultural Science and Biotechnology 87 (2012)6. - ISSN 1462-0316 - p. 654 - 660.
in-situ hybridization - x asiatic hybrids - sexual polyploidization - interspecific hybridization - intergenomic recombination - homoeologous recombination - bc2 progenies - lily hybrids - longiflorum - introgression
A total of 21 BC1 progenies of Oriental × Trumpet (OT) Lilium hybrids, nine BC2 progenies of OT hybrids, and three BC1 progenies of Martagon × Asiatic (MA) hybrids were analysed using genomic in situ hybridisation (GISH). Of the BC1 progenies of the OT hybrids, 15 were euploid (14 triploids, 2n = 3x = 36; 1 diploid, 2n = 2x = 24), and six were aneuploid. The triploid progenies had resulted from 2n eggs due to first division restitution (FDR). The aneuploid progenies had resulted from viable aneuploid gametes. All the BC2 progenies of triploid (2n = 3x = 36) OOT hybrids were aneuploid. Chromosome numbers in the BC2 progenies varied from 25 to 29.Two out of three BC1 progenies of the MA hybrids were aneuploids, with 35 and 32 chromosomes, respectively. Another was a triploid (2n = 3x = 36) which had resulted from 2n eggs due to indeterminate meiotic restitution (IMR). GISH revealed extensive intergenomic recombination in the BC1 progenies of OT and MA hybrids. A large number of Trumpet or Martagon chromosome segments were transmitted to the BC1 progenies from the F1 OT or MA hybrids. However, very few Trumpet chromosome segments were transmitted from the triploid BC1 parent to the BC2 progenies. These results indicate that it should be possible to select cultivars which possessed both traits from these BC1 progenies and they would be fertile.
Relevance of unilateral and bilateral sexual polyploidization in relation to intergenomic recombination and introgression in Lilium species hybrids
Nadeem Khan, M. ; Barba Gonzalez, R. ; Ramanna, M.S. ; Arens, P.F.P. ; Visser, R.G.F. ; Tuyl, J.M. van - \ 2010
Euphytica 171 (2010)2. - ISSN 0014-2336 - p. 157 - 173.
2n pollen formation - x asiatic hybrids - cytological mechanism - tetraploid progeny - genome composition - diploid potato - distant hybrid - bc2 progenies - gish analysis - lily hybrids
Sexual polyploids were induced in diploid (2n = 2x = 24) interspecific F1 hybrids of Longiflorum × Asiatic (LA) and Oriental × Asiatic (OA) Lilium hybrids by backcrossing to Asiatic (AA) parents as well as by sib-mating of the F1 LA hybrids. A majority of the BC1 progenies were triploid and the progenies from sib-mating were tetraploid or near tetraploids. Genomic in situ hybridization (GISH) technique was applied to assess the intergenomic recombination in the BC1 populations of LA and OA hybrids obtained after unilateral sexual polyploidization. A total of 63 LA (LA × AA and AA × LA) and 53 OA hybrids were analysed. LA hybrids were originated through the functioning of 2n gametes either as 2n eggs or 2n pollen while those of OA hybrids originated through functional 2n pollen of diploid OA genotype. In both type of crosses, a majority of the progenies had originated through First Division Restitution (FDR) mechanism of functional 2n gamete either with or without a cross over. However, there were nine LA- and four OA-genotypes where Indeterminate Meiotic Restitution (IMR) was the mechanism of 2n gamete formation. Based on GISH, total amount of introgression of Longiflorum and Oriental genome into Asiatic genome was determined. Most of the BC progenies exhibited recombination and the amount of recombination was higher in LA hybrids as compared to OA hybrids. Intergenomic recombination was also determined cytologically in the 16 plants of sib-mated LA hybrids where both parents had contributed 2n gametes. Based on these results the nature of interspecific lily hybrids obtained from uni- and bilateral sexual polyploidization leading to allotriploid and allotetraploid formation is discussed in the context of introgression and intergenomic recombination
Potential for analytic breeding in allopolyploids: An illustration from Longiflorum x Asiatic hybrid lilies (Lilium).
Nadeem Khan, M. ; Zhou, Shujun ; Ramanna, M.S. ; Arens, P.F.P. ; Herrera, Jeronimo ; Visser, R.G.F. ; Tuyl, J.M. van - \ 2009
Euphytica 166 (2009)3. - ISSN 0014-2336 - p. 399 - 409.
sexual polyploidization - interspecific hybrids - lolium-multiflorum - festuca-pratensis - bc2 progenies - gish analysis - lily hybrids - sweet-potato - 2n pollen - genome
Ploidy level and intergenomic recombination was studied in interspecific hybrids between Longiflorum × Asiatic lilies (LA hybrid) backcross to Asiatic parents in order to assess the possibility for analytic breeding in lily. By backcrossing the diploid (2n = 2x = 24) F1 interspecific hybrid between Longiflorum × Asiatic lilies to Asiatic parents, 104 BC1 progeny plants were produced. Among these, there were 27 diploids, 73 triploids (2n = 2x = 36) and 4 aneuploids (2x ¿ 1, 2x + 2 or 2x + 3). In addition, by backcrossing triploid BC1 (LAA) plants to diploid Asiatic parents in 2x ¿ 3x and reciprocal combinations, 14 diploid BC2 progenies were produced. Genomic in situ hybridization (GISH) was performed to study the intergenomic recombination and karyotype composition. GISH indicated extensive intergenomic recombination among the chromosomes in LA hybrids. A large number of Longiflorum chromosomes were transmitted to the BC1 progenies from LA hybrids. However, very few Longiflorum chromosomes were transmitted from the BC1 triploid (LAA) plants to the BC2 progenies. The occurrence of diploid plants in the BC progenies of LA hybrids has opened the prospects of analytic breeding in lilies. In this approach, the selection of superior genotypes can be carried out at the diploid level and polyploid forms are synthesized from superior diploid parents. The advantages of analytic breeding are evident: (a) a maximum level of heterozygosity can be attained in the synthetic polyploids and (b) introgression can be achieved with a minimum of linkage drag. Based on GISH results the potential application of analytic breeding in lily allopolyploids has been discussed
Analysis of the meiosis in the F-1 hybrids of Longiflorum x Asiatic (LA) of lilies (Lilium) using genomic in situ hybridization
Zhou, S.J. ; Ramanna, M.S. ; Visser, R.G.F. ; Tuyl, J.M. van - \ 2008
Journal of Genetics and Genomics 35 (2008)11. - ISSN 1673-8527 - p. 687 - 695.
intergenomic recombination - interspecific hybrids - parental genomes - allium-roylei - bc2 progenies - lily hybrids - gish - chromosomes - fistulosum - identification
Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaceae are two important groups of modem lily cultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the F-1 hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC1 progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC1 progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in F-1 hybrids of Longiflorum, x Asiatic (LA) hybrids in Lilium. Therefore, the meiotic process of ten F-1 LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research. The results showed that: at metaphase I, the homoeologous chromosome pairing among different F-1 hybrids ranged from 2.0 to 11.4 bivalents formed by homoeologous chromosomes per pollen mother cell (PMC), and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all bivalents were disjoined and most univalents were divided. Both the disjoined bivalents (half-bivalents) and the divided univalents (sister chromatids) moved to the opposite poles, and then formed two groups of chromosomes; because the two resulting half-bivalents retained their axes in the cell undisturbed, many crossover types, including single crossovers, three strand double crossovers, four strand double crossovers, four strand triple crossovers, and four strand multiple crossovers between the non-sister chromatids in the tetrads of bivalents, were clearly inferred by analyzing the breakpoints on the disjoined bivalents. The present investigation not only explained the reason for sterility of the F-1 LA hybrids and the variation of their BC1 progenies, but also provided a new method to analyze crossover types in other F-1 interspecific hybrids as well.
Meiotic polyploidization with homoeologous recombination induced by caffeine treatment in interspecific lily hybrids
Lim, K.B. ; Barba Gonzalez, R. ; Shujun Zhou, ; Ramanna, M.S. ; Tuyl, J.M. van - \ 2005
Korean Journal of Genetics 27 (2005)3. - ISSN 0254-5934 - p. 219 - 226.
lilium hybrids - bc2 progenies - restitution - cytokinesis - gish
Caffeine solution was injected into the flower bud to recover F, fertility of the intersectional diploid Lilium species hybrid (2n=2x=24). 0.3% of caffeine solution was the most effective concentration to produce fertile 2n-gametes. The male and female gametes had a range of fertility following caffeine injection in relation to the different sizes of flower buds. The gametes from the treated OA-hybrids showed to be fertile. They were crossed both as male and female parents to the Asiatics, and produced 279 BC, progeny plants. Flowcytometric and chromosomal observation confirmed that all progenies were triploid (2n=3x=36), indicating that the 2n-gametes from the OA-hybrid were functional following caffeine treatment. There was no homoeologous recombination between parental species in the progeny derived from male gametes of the OA-hybrid. However, three plants originating from the female gametes showed homoeologous recombination between Oriental and Asiatic genomes. The functional female gametes were produced when treated at the range of 20-23mm and 34-37mm of flower bud length. Based on the chromosome constitution of the triploid BC1 progenies, it was concluded that FDR 2n-gamete was functional and homoeologous recombination had occurred during the origin of 2n gametes.
Intergenomic recombination in F1 lily hybrids (Lilium) and its significance for genetic variation in the BC1 progenies as revealed by GISH and FISH
Barba Gonzalez, R. ; Ramanna, M.S. ; Visser, R.G.F. ; Tuyl, J.M. van - \ 2005
Genome 48 (2005)5. - ISSN 0831-2796 - p. 884 - 894.
bc2 progenies - crosses - pollination - relevance - culture - probe - pcr
Intergenomic recombination was assessed in a BC1 population of Oriental (O) × Asiatic (A) lilies (Lilium) backcrossed to Asiatic parents. This population consisted of 38 plants generated from the 2n gametes from 2 genotypes (951502-1 and 952400-1) of the diploid F1, Oriental × Asiatic lilies (2n = 2x = 24) as parents. In the majority of BC1 plants, there was evidence that first division restitution, with and without crossovers, resulted in functional gametes. However, there were 5 BC1 plants in which 2n gametes originated from indeterminate meiotic restitution (IMR). Based on the number of recombinant chromosomes for a particular homoeologous pair, 3 types of plants were identified: (i) those with both the reciprocal product of a crossover (O/A, A/O, where O represents the centromere of the O genome and A the recombinant segment of Asiatic chromosome, and vice versa); (ii) those with 1 normal chromosome of the O genome and a recombinant chromosome (O, A/O); and (iii) those with 1 normal chromosome of the A genome and a recombinant chromosome (A, O/A). An important feature of A × OA backcross progeny is the occurrence of substitutions for the segment distal in the crossover wherever the recombinant chromosome O/A was present. In the case of IMR, the substitution occurred for both proximal and distal recombinant segments. The significance of these substitutions is that they offer the potential for the phenotypic expression of recessive genes in polyploids (i.e., nulliplex genotype)
Occurrence of SDR 2N-gametes in Lilium hybrids
Lim, K.B. ; Shen, T.M. ; Barba Gonzalez, R. ; Ramanna, M.S. ; Tuyl, J.M. van - \ 2004
Breeding Science 54 (2004)1. - ISSN 1344-7610 - p. 13 - 18.
interspecific hybrids - bc2 progenies - potato - mutants - desynapsis - fertility - crosses - pollen - gish
The mechanism of SDR 2n-pollen formation was analyzed in two intra-sectional diploid (2n = 2x = 24) Lilium hybrids (Enchantment x L. pumilum). Variable frequencies of 2n-pollen were found. Meiotic analysis indicated that the intra-sectional hybrids showed perfect chromosome pairing in most cases at metaphase I and normal anaphase I movement of pollen mother cells (PMCs), but produced 2n-pollen by second division restitution (SDR). A high bivalent formation (11.9II and 11.8II, respectively) at metaphase 1, irregular meiotic division such as unbalanced chromosome separation and chromatic fragmentation resulted yet in acceptable pollen fertility for cross-pollination. The hybrids were fertile, and when used as male parents, offspring could tie generated. The significance of the occurrence of 2n-pollen for the breeding of lilies was analyzed.
Use of 2n gametes for the production of sexual polyploids from sterile Oriental x Asiatic hybrids of lilies (Lilium)
Barba Gonzalez, R. ; Lokker, A.C. ; Lim Ki-Byung, ; Ramanna, M.S. ; Tuyl, J.M. van - \ 2004
Theoretical and Applied Genetics 109 (2004)6. - ISSN 0040-5752 - p. 1125 - 1132.
situ hybridization gish - interspecific hybrids - homoeologous recombination - aegilops-squarrosa - bc2 progenies - alstroemeria - crosses - culture - restitution - chromosomes
Sixteen Oriental and 12 Asiatic cultivars were crossed in 158 different combinations. A total of 708 F1 hybrids were obtained from 86 of the different combinations of 15 Oriental and 11 Asiatic cultivars. Because the Lilium cultivars (2n=2x=24) used for the production of these hybrids belong to two different taxonomic sections-Archelirion (O) and Sinomartagon (A), respectively - the F1 hybrids (OA) could be obtained only through embryo, embryo sac rescue, ovary slice or ovule culture. Most of the F1 hybrids were highly sterile (did not produce viable n gametes) due to the failure of chromosome pairing. However, in a few cases F1 plants were found that produced viable 2n pollen at variable frequencies. These 2n pollen grains were successfully used for the production of backcross progenies. Using genomic in situ hybridization we found intergenomic recombinant chromosomes in the sexual polyploid progenies. These results indicate that there are effective prospects for combining important horticultural traits from the two main groups of cultivars of lilies through sexual polyploidization
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