Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

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    We will mail you new results for this query: keywords==honey-bee workers
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Miscellaneous standard methods for Apis mellifera research
Human, H. ; Brodschneider, R. ; Dietemann, V. ; Dively, G. ; Ellis, J.D. ; Forsgren, E. ; Fries, I. ; Hatjina, F. ; Hu, F.L. ; Jaffe, R. ; Jensen, A.B. ; Kohler, A. ; Magyar, J.P. ; Ouml;zkyrym, A. ; Pirk, C.W.W. ; Rose, R. ; Strauss, U. ; Tanner, G. ; Tarpy, D.R. ; Steen, J.J.M. van der; Vaudo, A. ; Vejsnaes, F. ; Wilde, J. de; Williams, G.R. ; Zheng, H.Q. - \ 2013
Journal of Apicultural Research 52 (2013)4. - ISSN 0021-8839
honey-bee workers - greatheadii var. davyana - low-temperature narcosis - varroa-jacobsoni oud - carbon-dioxide - nosema-ceranae - developmental stages - nutritional content - drone congregation - natural conditions
A variety of methods are used in honey bee research and differ depending on the level at which the research is conducted. On an individual level, the handling of individual honey bees, including the queen, larvae and pupae are required. There are different methods for the immobilising, killing and storing as well as determining individual weight of bees. The precise timing of developmental stages is also an important aspect of sampling individuals for experiments. In order to investigate and manipulate functional processes in honey bees, e. g. memory formation and retrieval and gene expression, microinjection is often used. A method that is used by both researchers and beekeepers is the marking of queens that serves not only to help to locate her during her life, but also enables the dating of queens. Creating multiple queen colonies allows the beekeeper to maintain spare queens, increase brood production or ask questions related to reproduction. On colony level, very useful techniques are the measurement of intra hive mortality using dead bee traps, weighing of full hives, collecting pollen and nectar, and digital monitoring of brood development via location recognition. At the population level, estimation of population density is essential to evaluate the health status and using beelines help to locate wild colonies. These methods, described in this paper, are especially valuable when investigating the effects of pesticide applications, environmental pollution and diseases on colony survival.
Standard methods for maintaining adult Apis mellifera in cages under in vitro laboratory conditions
Williams, G.R. ; Alaux, C. ; Costa, C. ; Csaki, C. ; Steen, J.J.M. van der - \ 2013
Journal of Apicultural Research 52 (2013)1. - ISSN 0021-8839
honey-bee workers - division-of-labor - nosema-ceranae microsporidia - proboscis extension response - juvenile-hormone - carbon-dioxide - paenibacillus-larvae - gamma-irradiation - pollen - colony
Adult honey bees are maintained in vitro in laboratory cages for a variety of purposes. For example, researchers may wish to perform experiments on honey bees caged individually or in groups to study aspects of parasitology, toxicology, or physiology under highly controlled conditions, or they may cage whole frames to obtain freshly emerged workers of known age cohorts. Regardless of purpose, researchers must manage a number of variables, ranging from selection of study subjects (e.g. honey bee subspecies) to experimental environment (e.g. temperature and relative humidity). Although decisions made by researchers may not necessarily jeopardize the scientific rigour of an experiment, they may profoundly affect results, and may make comparisons with similar, but independent, studies difficult. Focusing primarily on workers, we provide recommendations for maintaining adults under in vitro laboratory conditions, whilst acknowledging gaps in our understanding that require further attention. We specifically describe how to properly obtain honey bees, and how to choose appropriate cages, incubator conditions, and food to obtain biologically relevant and comparable experimental results. Additionally, we provide broad recommendations for experimental design and statistical analyses of data that arises from experiments using caged honey bees. The ultimate goal of this, and of all COLOSS BEEBOOK papers, is not to stifle science with restrictions, but rather to provide researchers with the appropriate tools to generate comparable data that will build upon our current understanding of honey bees
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