Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients
Goh, L.Y.H. ; Kam, Y.W. ; Metz, S.W.H. ; Hobson-Peters, J. ; Prow, N.A. ; McCarthy, S. ; Smith, D.W. ; Pijlman, G.P. ; Ng, L.F.P. ; Hall, R.A. - \ 2015
Journal of Virological Methods 222 (2015). - ISSN 0166-0934 - p. 55 - 61.
west-nile-virus - linked-immunosorbent-assay - valley encephalitis-virus - monoclonal-antibodies - diagnostic-accuracy - universal detection - reunion island - insect cells - ns1 protein - pcr assay
Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.
Development and validation of a rapid multiplex ELISA for pyrrolizidine alkaloids and their N-oxides in honey and feed
Oplatowska, M. ; Elliott, C.T. ; Huet, A.C. ; McCarthy, M. ; Mulder, P.P.J. ; Holst, C. von; Delahaut, P. ; Egmond, H.P. van; Campbell, K. - \ 2014
Analytical and Bioanalytical Chemistry 406 (2014)3. - ISSN 1618-2642 - p. 757 - 770.
linked-immunosorbent-assay - dietary-supplements - enzyme-immunoassay - mass-spectrometry - plants - food - toxicity - invitro - pollen - system
Pyrrolizidine alkaloids (PAs) are a group of plant secondary metabolites with carcinogenic and hepatotoxic properties. When PA-producing plants contaminate crops, toxins can be transferred through the food chain and cause illness in humans and animals, most notably hepatic veno-occlusive disease. Honey has been identified as a direct risk of human exposure. The European Food Safety Authority has recently identified four groups of PAs that are of particular importance for food and feed: senecionine-type, lycopsamine-type, heliotrine-type and monocrotaline-type. Liquid or gas chromatography methods are currently used to detect PAs but there are no rapid screening assays available commercially. Therefore, the aim of this study was to develop a rapid multiplex ELISA test for the representatives of three groups of alkaloids (senecionine, lycopsamine and heliotrine types) that would be used as a risk-management tool for the screening of these toxic compounds in food and feed. The method was validated for honey and feed matrices and was demonstrated to have a detection capability less than 25 µg/kg for jacobine, lycopsamine, heliotrine and senecionine. The zinc reduction step introduced to the extraction procedure allows for the additional detection of the presence of N-oxides of PAs. This first multiplex immunoassay for PA detection with N-oxide reduction can be used for the simultaneous screening of 21 samples for >12 PA analytes. Honey samples (n¿=¿146) from various origins were analysed for PA determination. Six samples were determined to contain measurable PAs >25 µg/kg by ELISA which correlated to >10 µg/kg by LC-MS/MS.
Evaluation of a Commercial ELISA for Detection of Ruminant Processed Animal Proteins in Non-Ruminant Processed Animal Proteins
Bremer, M.G.E.G. ; Margry, R.J.C.F. ; Vaessen, J.C.H. ; Doremalen, A.M.H. van; Palen, J.G.P. van der; Kaathoven, R.G.C. van; Kemmers-Voncken, A.E.M. ; Raamsdonk, L.W.D. van - \ 2013
Journal of AOAC International 96 (2013)3. - ISSN 1060-3271 - p. 552 - 559.
plant sterilization conditions - shrimp litopenaeus-vannamei - linked-immunosorbent-assay - bone meals - classical microscopy - rendered meat - by-products - feed - pcr - identification
Due to a growing aquaculture industry, demand for high-quality proteins for aquatic feeds is increasing. Non-ruminant processed animal proteins (PAPs) have shown great potential for this purpose. Safe reintroduction of non-ruminant PAPs in aqua feed requires methods that can discriminate ruminant and non-ruminant PAPs at contamination levels at or below 2%. Because the official European Union method lacks species specificity, the performance of MELISA-TEK™ Ruminant, a commercial immunoassay, combined with the MELISA-TEK High Sensitivity Sample Extraction kit was evaluated. Various non-ruminant PAPs spiked with ruminant PAPs (processed at 133, 137, 141, and 145°C) were analyzed. Results showed an overall specificity of 99%, indicating no cross-reaction with non-ruminant PAPs. The sensitivity of the assay strongly depended on both processing temperature and proportion of muscle fibers of the ruminant PAPs. Overall sensitivity of samples with 1 and 2% ruminant PAPs was 92 and 100%, respectively. For ruminant PAPs processed at 133 and 137°C, the sensitivity was 100% for both 1 and 2% ruminant spikes. Overall accuracies were 96 and 99% for 1 and 2% ruminant spikes, respectively. In conclusion, the MELISA-TEK Ruminant assay showed satisfactory results, which makes it a suitable candidate method to enable safe reintroduction of non-ruminant PAPs in aqua feed.
Colour-encoded paramagnetic microbead-based direct inhibition triplex flow cytometric immunoassay for ochratoxin A, fumonisins and zearalenone in cereals and cereal-based feed
Peters, J. ; Thomas, D. ; Boers, E.A.M. ; Rijk, T.C. de; Berthiller, F. ; Haasnoot, W. ; Nielen, M.W.F. - \ 2013
Analytical and Bioanalytical Chemistry 405 (2013)24. - ISSN 1618-2642 - p. 7783 - 7794.
linked-immunosorbent-assay - surface-plasmon resonance - mycotoxin analysis - natural occurrence - food - b-1 - products - maize - corn - regulations
A combined (triplex) immunoassay for the simultaneous detection of three mycotoxins in grains was developed with superparamagnetic colour-encoded microbeads, in combination with two bead-dedicated flow cytometers. Monoclonal antibodies were coupled to the beads, and the amounts of bound mycotoxins were inversely related to the amounts of bound fluorescent labelled mycotoxins (inhibition immunoassay format). The selected monoclonal antibodies were tested for their target mycotoxins and for cross-reactivity with relevant metabolites and masked mycotoxins. In the triplex format, low levels of cross-interactions between the assays occurred at irrelevant high levels only. All three assays were influenced by the sample matrix of cereal extracts to some extent, and matrix-matched calibrations are recommended for quantitative screening purposes. In a preliminary in-house validation, the triplex assay was found to be reproducible, sensitive and sufficiently accurate for the quantitative screening at ML level. The triplex assay was critically compared to liquid chromatography–tandem mass spectrometry using reference materials and fortified blank material. Results for the quantification of ochratoxin A and zearalenone were in good agreement. However, the fumonisin assay was, due to overestimation, only suitable for qualitative judgements. Both flow cytometer platforms (Luminex 100 and FLEXMAP 3D) performed similar with respect to sensitivity with the advantages of a higher sample throughput and response range of the FLEXMAP 3D and lower cost of the Luminex 100.
Screening methods and recent developments in the detection of anticoccidials
Huet, A.C. ; Bienenmann-Ploum, M.E. ; Vincent, U. ; Delahaut, P. - \ 2013
Analytical and Bioanalytical Chemistry 405 (2013)24. - ISSN 1618-2642 - p. 7733 - 7751.
tandem mass-spectrometry - performance liquid-chromatography - linked-immunosorbent-assay - time-resolved fluoroimmunoassay - fluorescence polarization immunoassay - uv spectrophotometric detection - antibody-based immunoassay - feed additive nicarbazin - chain v
This article presents a review of the current trends in the analysis of coccidiostats in various matrices, focusing principally on screening and rapid methods. Coccidiosis is an infectious disease having a high negative impact on the animal industry. Drugs are therefore necessary to prevent and/or to combat this disease. However, it is also of crucial importance that these veterinary drugs do not enter the human food chain. European legislation has therefore established the boundaries for the use of coccidiosats and has also addressed the unavoidable problem of cross-contamination of the feed, mainly caused by the use of the same production lines. Consequently there is a need for analytical methods and/or analytical strategies for the monitoring and control of the residues of anticoccidials, both in feed and in the resulting matrices for human consumption. In the frame of the European collaborative project CONffIDENCE, such attempts to establish the required analytical tools were made, which required beforehand a review of the state of the art in this domain. Aiming at this objective, in this review we consider themost interesting publications since 2000. In essence, both a rapid approach with mainly immunoassays and chromatographic methods were developed. To date, the obstacle to routine use of the first approach has been its inability to detect more than two compounds simultaneously, but recent developments in flow cytometry have made it possible to detect six coccidiostats at once. On the other hand, an increasingly popular approach for detecting multiple coccidiostats simultaneously is liquid chromatography coupledwith tandemmass spectrometry. There remains a need to adapt these analytical methods to legislative requirements.
Developments in mycotoxin analysis: an update for 2011-2012
Shephard, G.S. ; Berthiller, F. ; Burdaspal, P. ; Crews, C. ; Jonker, M.A. ; Krska, R. ; MacDonald, S. ; Malone, R.J. ; Maragos, C. ; Sabino, M. ; Solfrizzo, M. ; Egmond, H.P. van; Whitaker, T.B. - \ 2013
World Mycotoxin Journal 6 (2013)1. - ISSN 1875-0710 - p. 3 - 30.
performance liquid-chromatography - tandem mass-spectrometry - solid-phase extraction - immunoaffinity column cleanup - surface-plasmon resonance - aflatoxin m-1 contamination - linked-immunosorbent-assay - thin-layer-chromatography - lateral flow immunoassay - di
This review highlights developments in mycotoxin analysis and sampling over a period between mid-2011 and mid-2012. It covers the major mycotoxins aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxin, patulin, trichothecenes, and zearalenone. A section on mycotoxins in botanicals and spices is also included. Methods for mycotoxin determination continue to be developed using a wide range of analytical systems ranging from rapid immunochemical-based methods to the latest advances in tandem mass spectrometry. This review follows the format of previous reviews in this series (i.e. sections on individual mycotoxins), but due to the rapid spread and developments in the field of multimycotoxin methods by LC-MS/MS, a separate section has been devoted to advances in this area of research.
A bead-based suspension array for the serological detection of Trichinella in pigs
Wal, F.J. van der; Achterberg, R.P. ; Kant, A. ; Maassen, C.B.M. - \ 2013
The Veterinary Journal 196 (2013)3. - ISSN 1090-0233 - p. 439 - 444.
linked-immunosorbent-assay - multiplexed luminex assay - nonstructural proteins - antibodies - trichinosis - virus - spiralis - animals - serodiagnosis - immunoassay
The feasibility of using bead-based suspension arrays to detect serological evidence of Trichinella in pigs was assessed. Trichinella spiralis excretory–secretory antigen was covalently coupled to paramagnetic beads and used to bind serum antibodies, which were subsequently detected using anti-swine antibody. The assay was evaluated by testing pig sera from farms where trichinellosis was endemic and comparing the results with those obtained using two commercially available ELISAs. With cut-offs established by receiver operating characteristic (ROC) analysis, digestion-negative sera from a Trichinella-free population of pigs were deemed seronegative. When anti-swine antibody was replaced with protein A/G, higher test sensitivity (94% vs. 88%) at similar test specificity (95%), was achieved. The potential use of this assay in species other than swine was also demonstrated by testing human sera.
European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus
Kortekaas, J.A. ; Kant, J. ; Vloet, R.P.M. ; Cêtre-Sossah, C. ; Marianneau, P. ; Lacote, S. ; Banyard, A.C. ; Jeffries, C. ; Eiden, M. ; Groschup, M. ; Jäckel, S. ; Hevia, E. ; Brun, A. - \ 2013
Journal of Virological Methods 187 (2013)1. - ISSN 0166-0934 - p. 177 - 181.
linked-immunosorbent-assay - reverse transcription-pcr - nucleocapsid protein - domestic ruminants - capture elisa - igg antibody - validation - sandwich - humans - sheep
A ring trial was organized to evaluate Rift Valley fever virus (RVFV) ELISAs by European laboratories. A total of five ELISAs, two of which specific for IgM antibodies, were evaluated by six participants. Sera were derived from cattle or sheep and originated from either a RVFV endemic area, a RVFV-free area or from experimental infection studies. Cohen's kappa analysis showed higher than 90% agreement of two commercially available ELISAs with the virus neutralization test, suggesting that primary screening as well as serological confirmation using these ELISAs is feasible. More extensive validations with sera of known IgM status are, however, required to determine agreement between IgM ELISAs.
Improved functional immobilization of llama single-domain antibody fragments to polystyrene surfaces using small peptides
Harmsen, M.M. ; Fijten, H.P.D. - \ 2012
Journal of Immunoassay and Immunochemistry 33 (2012)3. - ISSN 1532-1819 - p. 234 - 251.
linked-immunosorbent-assay - escherichia-coli - passive-immunization - mouth-disease - in-vitro - microarrays - strategies - bivalent - pigs - hydrophobins
We studied the effect of different fusion domains on the functional immobilization of three llama single-domain antibody fragments (VHHs) after passive adsorption to polystyrene in enzyme-linked immunosorbent assays (ELISA). Three VHHs produced without any fusion domain were efficiently adsorbed to polystyrene, which, however, resulted in inefficient antigen binding. Functional VHH immobilization was improved by VHH fusion to a consecutive myc-His6-tag and was even more improved by fusion to the llama antibody long hinge region containing an additional His6-tag (LHc-His6). The partial dimerization of VHH-LHc-His6 fusion proteins through LHc-mediated disulfide-bond formation was not essential for their improved functional immobilization. VHH fusions to specific polystyrene binding peptides, hydrophobins, or other, unrelated VHH domains were less suitable for increasing functional VHH immobilization because of reduced microbial expression levels. Thus, VHH-LHc-His6 fusion proteins are most suited for functional passive adsorption in ELISA.
Bead-based suspension array for simultaneous detection of antibodies against the Rift Valley fever virus nucleocapsid and Gn glycoprotein
Wal, F.J. van der; Achterberg, R.P. ; Boer, S.M. de; Boshra, H. ; Brun, A. ; Maassen, C.B.M. ; Kortekaas, J.A. - \ 2012
Journal of Virological Methods 183 (2012)2. - ISSN 0166-0934 - p. 99 - 105.
linked-immunosorbent-assay - domestic ruminants - indirect elisa - igm antibodies - capture elisa - nss protein - n-protein - humans - validation - sandwich
A multiplex bead-based suspension array was developed that can be used for the simultaneous detection of antibodies against the surface glycoprotein Gn and the nucleocapsid protein N of Rift Valley fever virus (RVFV) in various animal species. The N protein and the purified ectodomain of the Gn protein were covalently linked to paramagnetic Luminex beads. The performance of the resulting multiplex immunoassay was evaluated by testing a comprehensive and well-characterized panel of sera from sheep, cattle and humans. The suitability of this multiplex immunoassay to differentiate infected from vaccinated animals (DIVA) was investigated by testing sera from lambs vaccinated with a paramyxovirus vaccine vector expressing the RVFV surface glycoproteins Gn and Gc. The results suggest that the bead-based suspension array can be used as a DIVA assay to accompany several recently developed experimental vaccines that are based on RVFV glycoproteins, and are devoid of the N protein.
Consensus based reporting standards for diagnostic test accuracy studies for paratuberculosis in ruminants.
Gardner, I.A. ; Nielsen, S.S. ; Whittington, R.J. ; Collins, M.T. ; Bakker, D. ; Harris, B. ; Sreevatsan, S. ; Lombard, J.E. ; Sweeney, R. ; Smith, D.R. ; Gavalchin, J. ; Eda, S. - \ 2011
Preventive Veterinary Medicine 101 (2011)1-2. - ISSN 0167-5877 - p. 18 - 34.
avium subsp-paratuberculosis - linked-immunosorbent-assay - pooled fecal culture - real-time pcr - mycobacterium-avium - johnes-disease - dairy-cattle - ovine paratuberculosis - bovine-paratuberculosis - serological tests
The Standards for Reporting of Diagnostic Accuracy (STARD) statement ( was developed to encourage complete and transparent reporting of key elements of test accuracy studies in human medicine. The statement was motivated by widespread evidence of bias in test accuracy studies and the finding that incomplete or absent reporting of items in the STARD checklist was associated with overly optimistic estimates of test performance characteristics. Although STARD principles apply broadly, specific guidelines do not exist to account for unique considerations in livestock studies such as herd tests, potential use of experimental challenge studies, a more diverse group of testing purposes and sampling designs, and the widespread lack of an ante-mortem reference standard with high sensitivity and specificity. The objective of the present study was to develop a modified version of STARD relevant to paratuberculosis (Johne's disease) in ruminants. Examples and elaborations for each of the 25 items were developed by a panel of experts using a consensus-based approach to explain the items and underlying concepts. The new guidelines, termed STRADAS-paraTB (Standards for Reporting of Animal Diagnostic Accuracy Studies for paratuberculosis), should facilitate improved quality of reporting of the design, conduct and results of paratuberculosis test accuracy studies which were identified as "poor" in a review published in 2008 in Veterinary Microbiology
Differentiation of Foot-and-Mouth Disease-Infected pigs from Vaccinated Pigs Using Antibody-Detecting Sandwich ELISA
Chen, T.H. ; Lee, F. ; Lin, Y.L. ; Dekker, A. ; Chung, W.B. ; Pan, C.H. ; Jong, M.H. ; Huang, C.C. ; Lee, M.C. ; Tsai, H.J. - \ 2011
Journal of Veterinary Medical Science 73 (2011)8. - ISSN 0916-7250 - p. 977 - 984.
nonstructural polyprotein 3abc - linked-immunosorbent-assay - monoclonal-antibody - virus-infection - proteins - antigen - cattle - recombinant - eradication - baculovirus
The presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for rapid detection of the foot-and-mouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC nonstructural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. The diagnostic sensitivity of the assay was 98.4%, and the diagnostic specificity was 100% for naïve and vaccinated pigs; the detection ability of the assay was comparable those of the PrioCHECK and UBI kits. There was 97.5, 93.4 and 66.6% agreement between the results obtained from our ELISA and those obtained from the PrioCHECK, UBI and CHEKIT kits, respectively. The kappa statistics were 0.95, 0.87 and 0.37, respectively. Moreover, antibodies for nonstructural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2 and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assay's specificity
Imaging surface plasmon resonance for multiplex microassay sensing of mycotoxins
Dorokhin, D. ; Haasnoot, W. ; Franssen, M.C.R. ; Zuilhof, H. ; Nielen, M.W.F. - \ 2011
Analytical and Bioanalytical Chemistry 400 (2011)9. - ISSN 1618-2642 - p. 3005 - 3011.
linked-immunosorbent-assay - fluorescence polarization immunoassay - tandem mass-spectrometry - monoclonal-antibodies - liquid-chromatography - gas-chromatography - rapid detection - deoxynivalenol - wheat - toxin
A prototype imaging surface plasmon resonance-based multiplex microimmunoassay for mycotoxins is described. A microarray of mycotoxin–protein conjugates was fabricated using a continuous flow microspotter device. A competitive inhibition immunoassay format was developed for the simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN), using a single sensor chip. Initial in-house validation showed limits of detection of 21 and 17 ng/mL for DON and 16 and 10 ng/mL for ZEN in extracts, which corresponds to 84 and 68 µg/kg for DON and 64 and 40 µg/kg for ZEN in maize and wheat samples, respectively. Finally, the results were critically compared with data obtained from liquid chromatography-mass spectrometry confirmatory analysis method and found to be in good agreement. The described multiplex immunoassay for the rapid screening of several mycotoxins meets European Union regulatory limits and represents a robust platform for mycotoxin analysis in food and feed samples
Single Laboratory Validation of a Surface Plasmon Resonance Biosensor Screening method for Paralytic Shellfish Poisoning Toxins
Campbell, K. ; Haughey, S.A. ; Top, H.J. van den; Egmond, H.J. van; Vilarino, N. ; Botana, L.M. ; Elliott, C.T. - \ 2010
Analytical Chemistry 82 (2010)7. - ISSN 0003-2700 - p. 2977 - 2988.
linked-immunosorbent-assay - prechromatographic oxidation - quantitative-determination - fluorescence detection - liquid-chromatography - enzyme-immunoassay - saxitoxin - neosaxitoxin - antibodies
A research element of the European Union (EU) sixth Framework project BioCop focused on the development of a surface plasmon resonance (SPR) biosensor assay for the detection of paralytic shellfish poisoning (PSP) toxins in shellfish as an alternative to the increasingly ethically unacceptable mouse bioassay. A biosensor assay was developed using both a saxitoxin binding protein and chip surface in tandem with a highly efficient simple extraction procedure. The present report describes the single laboratory validation of this immunological screening method, for this complex group of toxins with differing toxicities, according to the European Decision 2002/657/EC in conjunction with IUPAC and AOAC single laboratory validation guidelines. The different performance characteristics (detection capability CC beta, specificity/selectivity, repeatability, reproducibility, stability, and applicability) were determined in relation to the EU regulatory limit of 800 mu g of saxitoxin equivalents (STX eq) per kg of shellfish meat. The detection capability CC beta was calculated to be 120 mu g/kg. Intra-assay repeatability was found to be between 2.5 and 12.3% and interassay reproducibility was between 6.1 and 15.2% for different shellfish matrices. Natural samples were also evaluated and the resultant data displayed overall agreements of 96 and 92% with that of the existing AOAC approved methods of mouse bioassay (MBA) and high performance liquid chromatography (HPLC), respectively.
Developments in mycotoxin analysis: an update for 2008-2009
Shephard, G.S. ; Berthiller, F. ; Dorner, J. ; Krska, R. ; Lombaert, G.A. ; Malone, B. ; Maragos, C. ; Sabino, M. ; Solfrizzo, M. ; Trucksess, M.W. ; Egmond, H.P. van; Whitaker, T.B. - \ 2010
World Mycotoxin Journal 3 (2010)1. - ISSN 1875-0710 - p. 3 - 23.
performance liquid-chromatography - linked-immunosorbent-assay - tandem mass-spectrometry - corn-based food - immunoaffinity column cleanup - fluorescence detection method - single-laboratory validation - near-infrared spectroscopy - resorcylic acid lactones - afl
This review highlights developments in mycotoxin analysis and sampling over a period between mid-2008 and mid-2009. It covers the major mycotoxins: aflatoxins, alternaria toxins, cyclopiazonic acid, fumonisins, ochratoxin, patulin, trichothecenes and zearalenone. Developments in mycotoxin analysis continue, with emphasis on novel immunological methods and further description of LC-MS and LC-MS/MS, particularly as multimycotoxin applications for different ranges of mycotoxins. Although falling outside the main emphasis of the review, some aspects of natural occurrence have been mentioned, especially if linked to novel method developments.
Immunity to Campylobacter: its role in risk assessment and epidemiology
Havelaar, A.H. ; Pelt, W. van; Ang, C.W. ; Wagenaar, J.A. ; Putten, J.P.M. van; Gross, U. ; Newell, D.G. - \ 2009
Critical Reviews in Microbiology 35 (2009)1. - ISSN 1040-841X - p. 1 - 22.
guillain-barre-syndrome - intestinal epithelial-cells - human-antibody response - reactive arthritis patients - inflammatory-bowel-disease - linked-immunosorbent-assay - outer-membrane proteins - western-blot-analysis - jejuni infection - dose-response
Acquired immunity is an important factor in the epidemiology of campylobacteriosis in the developing world, apparently limiting symptomatic infection to children of less than two years. However, also in developed countries the highest incidence is observed in children under five years and the majority of Campylobacter infections are asymptomatic, which may be related to the effects of immunity and/or the ingested doses. Not accounting for immunity in epidemiological studies may lead to biased results due to the misclassification of Campylobacter-exposed but apparently healthy persons as unexposed. In risk assessment studies, health risks may be overestimated when immunity is neglected.
Label-Free and Multiplex Detection of Antibiotic Residues in Milk Using Imaging surface Plasmon Resonance-Based immunosensor
Rebe, S. ; Bremer, M.G.E.G. ; Haasnoot, W. ; Norde, W. - \ 2009
Analytical Chemistry 81 (2009)18. - ISSN 0003-2700 - p. 7743 - 7749.
linked-immunosorbent-assay - beta-lactam antibiotics - biosensor immunoassay - food-products - drug residues - fluoroquinolones - aminoglycosides - antibody - muscle - tests
Monitoring of antimicrobial drug residues in foods relies greatly on the availability of adequate analytical techniques. Currently, there is a need for a high-throughput screening method with a broad-spectrum detection range. This paper describes the development of a microarray biosensor, based on an imaging surface plasmon resonance (iSPR) platform, for quantitative and simultaneous immunodetection of different antibiotic residues in milk. Model compounds from four major antibiotic families: aminoglycosides (Neomycin, Gentamicin, Kanamycin, and Streptomycin), sulfonamides (Sulfamethazine), fenicols (Chloramphenicol), and fluoroquinolones (Enrofloxacin) were detected using a single sensor chip. By multiplexing seven immunoassays in a competitive format, we were able to measure all the target compounds at parts per billion (ppb) levels in buffer and in 10×-diluted milk. The assays for Neomycin, Kanamycin, Streptomycin, Enrofloxacin, and Sulfamethazine were sensitive enough for milk control at maximum residue levels as established in the European Union. The overall performance of the biosensor was determined to be comparable to that of conventional four-channel surface plasmon resonance (SPR)-based biosensors, in terms of assay sensitivity and robustness. Combining the advantages of a SPR sensor and a microarray, utilization of the biosensor described here offers a promising alternative to the existing methods and is highly relevant for multianalyte food profiling.
Seroprevalence of antibodies against pestiviruses in small ruminants in the Netherlands
Orsel, K. ; Antonis, A.F.G. ; Oosterloo, J.C. ; Vellema, P. ; Meer, F.J.U.M. van der - \ 2009
Tijdschrift voor Diergeneeskunde 134 (2009)9. - ISSN 0040-7453 - p. 380 - 384.
antilichamen - peste-des-petits-ruminants virus - seroprevalentie - antibodies - seroprevalence - border disease virus - linked-immunosorbent-assay - bovine viral diarrhea - sheep - cattle - transmission - goats - herpesvirus-1 - infection - bvdv
In this study, a serological survey was performed to determine the prevalence of pestivirus (bovine viral diarrhea virus (BVDV) and border disease virus (BDV)) infected small ruminants herds in the Netherlands. After random selection of sheep farms, a sample size was determined to detect a 5% herd prevalence. 13 out of 29 farms were tested seropositive using an ELISA which detects antibodies directed against the non structural protein 3 (NS3) of pestiviruses. This resulted in a seroprevalence for the Netherlands of 45% [0.36; 0.54]. The within farm prevalence ranged from 4 till 65%. Using a virus neutralization assay, specific anti-BDV antibodies could be detected on two farms, while on one other farm anti-BVDV antibodies were present. On four farms antibodies to both viruses could be detected, on three of these farms antibodies against both viruses were equally present. At five farms that tested positive in the NS3-ELISA we were unable to detect pestivirus neutralizing antibodies in all sera using the VN test. This resulted in an estimated prevalence using the VN for the Netherlands of 28% [0.20; 0.60]. An additional survey in sera from dairy goats revealed that 34 out of 126 farms were serological positive resulting in a seroprevalence of 27% [0.23; 0.31], with a herd prevalence of 32% ranging from 1-100%.
Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey
Posthuma-Trumpie, G.A. ; Korf, J. ; Amerongen, A. van - \ 2009
Analytical and Bioanalytical Chemistry 393 (2009)2. - ISSN 1618-2642 - p. 569 - 582.
converting phosphor technology - competitive immunochromatographic assay - immunoglobulin-m antibodies - linked-immunosorbent-assay - dipstick dye immunoassay - dry reagent immunoassay - computer image-analysis - fungal alpha-amylase - step strip test - rapid dete
Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for 'strengths, weaknesses, opportunities, threats'). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included 'immunochromatography', 'sol particle immunoassay', 'lateral flow immunoassay' and 'dipstick assay'.
Development of a competitive lateral flow immunoassay for progesterone: influence of coating conjugates and buffer components
Posthuma-Trumpie, G.A. ; Korf, J. ; Amerongen, A. van - \ 2008
Analytical and Bioanalytical Chemistry 392 (2008)6. - ISSN 1618-2642 - p. 1215 - 1223.
linked-immunosorbent-assay - mass-spectrometry - milk - samples - plasma - elisa - water
Several aspects of the development of competitive lateral flow immunoassays (LFIAs) are described. The quantitation of progesterone is taken as an example. The LFIA format consisted of a nitrocellulose membrane spotted with various progesterone conjugates as the test line. A mixture of primary antibody and secondary antibody adsorbed to colloidal carbon was used for signal generation. A digital scanner and dedicated software were used to quantitate the response. A reappraisal of the checkerboard titration, often used in the optimisation of immunoassays, is discussed. Surprisingly, the highest sensitivity of the LFIA format (IC50 of 0.6 µg L¿1 progesterone in buffer) was achieved by using a high coating concentration of the analyte¿protein conjugate and a high dilution of the antibody solution. Immediate addition of all reagents in LFIA was superior to premixing the components and allowing prereaction. Of several blocking agents tested bovine serum albumin was superior in performance, whereas the combination of ovalbumin and progesterone substantially influenced test results.
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