Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Plant Cytokinesis : Terminology for Structures and Processes
Smertenko, Andrei ; Assaad, Farhah ; Baluška, František ; Bezanilla, Magdalena ; Buschmann, Henrik ; Drakakaki, Georgia ; Hauser, Marie Theres ; Janson, Marcel ; Mineyuki, Yoshinobu ; Moore, Ian ; Müller, Sabine ; Murata, Takashi ; Otegui, Marisa S. ; Panteris, Emmanuel ; Rasmussen, Carolyn ; Schmit, Anne Catherine ; Šamaj, Jozef ; Samuels, Lacey ; Staehelin, L.A. ; Damme, Daniel Van; Wasteneys, Geoffrey ; Žárský, Viktor - \ 2017
Trends in Cell Biology 27 (2017)12. - ISSN 0962-8924 - p. 885 - 894.
Cell plate - Cytokinesis - Division plane - Phragmoplast - Preprophase band

Plant cytokinesis is orchestrated by a specialized structure, the phragmoplast. The phragmoplast first occurred in representatives of Charophyte algae and then became the main division apparatus in land plants. Major cellular activities, including cytoskeletal dynamics, vesicle trafficking, membrane assembly, and cell wall biosynthesis, cooperate in the phragmoplast under the guidance of a complex signaling network. Furthermore, the phragmoplast combines plant-specific features with the conserved cytokinetic processes of animals, fungi, and protists. As such, the phragmoplast represents a useful system for understanding both plant cell dynamics and the evolution of cytokinesis. We recognize that future research and knowledge transfer into other fields would benefit from standardized terminology. Here, we propose such a lexicon of terminology for specific structures and processes associated with plant cytokinesis. A large number of phragmoplast proteins have been identified.Electron microscopy/tomography studies have produced nanoscale information about the architecture of phragmoplast and cell plate assembly stages in cryofixed cells.Novel components of the cortical division zone and cell plate fusion site have been discovered. This information lays a foundation for understanding how plant cells memorize the division plane throughout mitosis and how the cell plate is guided to its predetermined attachment site.MAP65 and plus end-directed kinesins contribute to the maintenance of the antiparallel overlap of phragmoplast microtubules. In addition, the MAP65-TRAPPII interaction plays a key role in cell plate assembly.Actin filaments align parallel to microtubules in the phragmoplast, while some microfilaments extend from cell plate margin to guide its expansion towards the fusion site.

Genome-wide analysis of the barley MAPK gene family and its expression patterns in relation to Puccinia hordei infection
Křenek, Pavel ; Niks, Rients E. ; Vels, Anton ; Vyplelová, Petra ; Šamaj, Jozef - \ 2015
Acta Physiologiae Plantarum 37 (2015)11. - ISSN 0137-5881 - p. 1 - 16.
Barley (Hordeumvulgare) - Barley leaf rust (Pucciniahordei) - Effector-triggered immunity (ETI) - Mitogen-activated protein kinase (MAPK) - qRT-PCR - Resistance

Mitogen-activated protein kinases (MAPKs) have been shown to act as key regulators of stress responses in model plant and crop species. So far, however, the MAPK family has not been systematically studied in barley. Herein, we identified 16 HvMAPKs (Hv—Hordeum vulgare) based on computational analysis of barley transcriptomics and genomics databases. HvMAPKs contain all canonical MAPK domains, except for HvMPK2, which lacks a MAPK domain signature. In addition, five HvMAPKs harbor TEY and ten HvMAPKs harbor TDY dual phosphorylation motif in the activation loop. Interestingly, HvMPK2 contains a MEY instead of TEY phosphorylation motif. We classified HvMAPKs into four major plant MAPK clades based on phylogeny reconstruction and anchored all HvMAPK genes to five out of seven barley chromosomes. Furthermore, we inoculated seedlings of susceptible barley line L94 and its isolines L94-Rph3 and L94-Rph7 with rust fungus Pucciniahordei and analyzed the expression of 16 HvMAPK genes using qRT-PCR at 1–4.5 days post inoculation. In total, six HvMAPK genes exhibited significantly altered expression by P. hordei infection. The expression of HvMPK5, HvMPK6, HvMPK7 and HvMPK12 (set one genes) was strongly induced especially during effector-triggered immunity (ETI), whereas the expression of HvMPK2 and HvMPK17 (set two genes) was specifically downregulated during ETI. Yet the expression of HvMPK8 was also specifically but weakly downregulated during ETI. Overall, the expression patterns suggest that set one genes positively regulate ETI in barley–P. hordei pathosystem, whereas set two genes negatively regulate ETI and/or programmed cell death in this pathosystem.

Endocytic accommodation of microbes in plants
Huisman, R. ; Ovchinnikova, E. ; Bisseling, T. ; Limpens, E.H.M. - \ 2012
In: Endocytosis in plants / Šamaj, J., Berlin Heidelberg : Springer Verlag - ISBN 9783642324635 - p. 271 - 295.
Plants host many different microbes within their cells. These endosymbiotic relationships are characterized by the formation of new specialized membrane compartments inside the plant cells in which the microbes live and where nutrients and signals are efficiently exchanged. Such symbiotic interfaces include arbuscules produced by arbuscular mycorrhiza (AM), organelle-like symbiosomes formed during the rhizobium-legume symbiosis, and haustoria produced by biotrophic fungi and oomycetes. The formation and maintenance of such new membrane compartments require a major reorganization of the host endomembrane system. In the last decade, much progress has been made in understanding how arbuscules, symbiosomes, and haustoria are formed. In this chapter, we will summarize the recent developments in each field, with a major focus on the AM and rhizobial endosymbiosis. It has become clear that rhizobia have co-opted a signalling pathway as well as a cellular mechanism to make the interface membrane compartment from the ancient and most successful AM symbiosis. Both AM symbiosis and rhizobium symbiosis depend on the secretion of lipo-chito-oligosaccharides that trigger a symbiotic signalling cascade, which is required for both arbuscule and symbiosome formation. In both interactions a shared specific exocytosis pathway is recruited to facilitate the formation of the symbiotic interface resulting in a membrane compartment with distinct protein composition. Given the structural similarity of haustoria to arbuscules, similar mechanisms are envisioned to be involved in the formation of a haustorium.
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