- Vicenta Garcia-Campayo (1)
- M.K.C. Hesselink (1)
- Hans J.M. Swarts (1)
- Jaap Keijer (1)
- Sander Kersten (1)
- Prokopis Konstanti (1)
- Evert M. Schothorst van (1)
- Lianne M.S. Bouwman (1)
- José M.S. Fernández-Calleja (1)
- Annemarie Oosting (1)
- S.H. Parekh (1)
- William Rock (1)
- Patrick Schrauwen (1)
- Hauke Smidt (1)
Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
Fernández-Calleja, José M.S. ; Konstanti, Prokopis ; Swarts, Hans J.M. ; Bouwman, Lianne M.S. ; Garcia-Campayo, Vicenta ; Billecke, Nils ; Oosting, Annemarie ; Smidt, Hauke ; Keijer, Jaap ; Schothorst, Evert M. van - \ 2018
Scientific Reports 8 (2018)1. - ISSN 2045-2322 - 16 p.
Real time in vivo methods are needed to better understand the interplay between diet and the gastrointestinal microbiota. Therefore, a rodent indirect calorimetry system was equipped with hydrogen (H2) and methane (CH4) sensors. H2 production was readily detected in C57BL/6J mice and followed a circadian rhythm. H2 production was increased within 12 hours after first exposure to a lowly-digestible starch diet (LDD) compared to a highly-digestible starch diet (HDD). Marked differences were observed in the faecal microbiota of animals fed the LDD and HDD diets. H2 was identified as a key variable explaining the variation in microbial communities, with specific taxa (including Bacteroides and Parasutterella) correlating with H2 production upon LDD-feeding. CH4 production was undetectable which was in line with absence of CH4 producers in the gut. We conclude that real-time in vivo monitoring of gases provides a non-invasive time-resolved system to explore the interplay between nutrition and gut microbes in a mouse model, and demonstrates potential for translation to other animal models and human studies.
Perilipin 5 mediated lipid droplet remodelling revealed by coherent Raman imaging
Billecke, Nils ; Bosma, Madeleen ; Rock, William ; Fleissner, Frederik ; Best, Gerrit ; Schrauwen, Patrick ; Kersten, Sander ; Bonn, Mischa ; Hesselink, M.K.C. ; Parekh, S.H. - \ 2015
Integrative Biology (United Kingdom) 7 (2015)4. - ISSN 1757-9694 - p. 467 - 476.
Accumulation of fat in muscle tissue as intramyocellular lipids (IMCLs) is closely related to the development of insulin resistance and subsequent type 2 diabetes. Most IMCLs organize into lipid droplets (LDs), the fates of which are regulated by lipid droplet coat proteins. Perilipin 5 (PLIN5) is an LD coating protein, which is strongly linked to lipid storage in muscle tissue. Here we employ a tandem in vitro/ex vivo approach and use chemical imaging by label-free, hyperspectral coherent Raman microscopy to quantify compositional changes in individual LDs upon PLIN5 overexpression. Our results directly show that PLIN5 overexpression in muscle alters individual LD composition and physiology, resulting in larger LDs with higher esterified acyl chain concentration, increased methylene content, and more saturated lipid species. These results suggest that lipotoxic protection afforded by natural PLIN5 upregulation in muscle involves molecular changes in lipid composition within LDs. This journal is