Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Records 1 - 20 / 609

  • help
  • print

    Print search results

  • export
    A maximum of 250 titles can be exported. Please, refine your queryYou can also select and export up to 30 titles via your marked list.
  • alert
    We will mail you new results for this query: q=Brand
Check title to add to marked list
Values and value conflicts in snack providing of Dutch, Polish, Indonesian and Italian mothers
Damen, Femke W.M. ; Hofstede, Gert Jan ; Steenbekkers, Bea L.P.A. ; Vitaglione, Paola ; Pellegrini, Nicoletta ; Fogliano, Vincenzo ; Luning, Pieternel A. - \ 2018
Food Research International (2018). - ISSN 0963-9969 - 8 p.
Child - Childhood obesity - Children's dietary behavior - Cross-cultural differences - Culture - Food choice - Mother - National culture

This study investigates which values play a role in the decision of mothers about snacks to offer to their young children with a focus on the value conflicts that might occur. The study explores whether national culture is reflected in mothers' values in snack choice for their young children and the related value conflicts. Semi-structured interviews with 67 mothers of 2–7 years old children divided over 4 national cultures (Dutch, Polish, Indonesian and Italian) were conducted. Questions were asked about their values and value conflicts when providing a snack to their young children. Four key themes could be distinguished to cluster the mentioned values. The health-related key theme includes all values that are associated with the healthiness of the product, the child-related key theme all values that connects to the child, the time-related key theme includes the value convenience and the product-related key theme includes all values that are associated with the product itself. Dutch and Polish mothers mostly valued health of the snack, whereas Indonesian and Italian mothers mostly valued the preference of their child. Data also shows specific prevalence between values and nationalities: convenience was very important for Dutch mothers, valuing organic food was typical for Polish mothers, religion played a role for Indonesian mothers, while Italian mothers placed more value on brand compared to the mothers of other cultures. In all cultures, the value conflicts mentioned were mainly related to health.

Cultivating change: How a Dutch university is working on a new agricultural revolution – to feed the world
Ende, Ernst van den; Marcelis, Leo ; Kema, Gert ; Zedde, Rick van de; Goot, Atze Jan van der - \ 2018
Demographic and Social-Cognitive Factors Associated with Weight Loss in Overweight, Pre-diabetic Participants of the PREVIEW Study
Hansen, Sylvia ; Huttunen-Lenz, Maija ; Sluik, Diewertje ; Brand-Miller, Jennie ; Drummen, Mathijs ; Fogelholm, Mikael ; Handjieva-Darlenska, Teodora ; Macdonald, Ian ; Martinez, Alfredo J. ; Larsen, Thomas Meinert ; Poppitt, Sally ; Raben, Anne ; Schlicht, Wolfgang - \ 2018
International Journal of Behavioral Medicine (2018). - ISSN 1070-5503 - 11 p.
Behavioral determination - Lifestyle intervention - Social-cognitive factors - Weight loss

Purpose: Weight loss has been demonstrated to be a successful strategy in diabetes prevention. Although weight loss is greatly influenced by dietary behaviors, social-cognitive factors play an important role in behavioral determination. This study aimed to identify demographic and social-cognitive factors (intention, self-efficacy, outcome expectancies, social support, and motivation with regard to dietary behavior and goal adjustment) associated with weight loss in overweight and obese participants from the PREVIEW study who had pre-diabetes. Method: Prospective correlational data from 1973 adult participants were analyzed. The participants completed psychological questionnaires that assessed social-cognitive variables with regard to dietary behavior. Stepwise multiple regression analyses were performed to identify baseline demographic and social-cognitive factors associated with weight loss. Results: Overall, being male, having a higher baseline BMI, having a higher income, perceiving fewer disadvantages of a healthy diet (outcome expectancies), experiencing less discouragement for healthy eating by family and friends (social support), and lower education were independently linked to greater weight loss. When evaluating females and males separately, education was no longer associated with weight loss. Conclusion: The results indicate that a supportive environment in which family members and friends avoid discouraging healthy eating, with the application of a strategy that uses specific behavior change techniques to emphasize the benefits of outcomes, i.e., the benefits of a healthy diet, may support weight loss efforts. Weight loss programs should therefore always address the social environment of persons who try to lose body weight because family members and friends can be important supporters in reaching a weight loss goal.

Beached bachelors: An extensive study on the largest recorded sperm whale Physeter macrocephalus mortality event in the North Sea
IJsseldijk, Lonneke L. ; Neer, Abbo van; Deaville, Rob ; Begeman, Lineke ; Bildt, Marco van de; Brand, Judith M.A. van den; Brownlow, Andrew ; Czeck, Richard ; Dabin, Willy ; Doeschate, Mariel ten; Herder, Vanessa ; Herr, Helena ; IJzer, Jooske ; Jauniaux, Thierry ; Jensen, Lasse Fast ; Jepson, Paul D. ; Jo, Wendy Karen ; Lakemeyer, Jan ; Lehnert, Kristina ; Leopold, Mardik F. ; Osterhaus, Albert ; Perkins, Matthew W. ; Piatkowski, Uwe ; Prenger-Berninghoff, Ellen ; Pund, Ralf ; Wohlsein, Peter ; Gröne, Andrea ; Siebert, Ursula - \ 2018
PLoS One 13 (2018)8. - ISSN 1932-6203

Between the 8th January and the 25th February 2016, the largest sperm whale Physeter macrocephalus mortality event ever recorded in the North Sea occurred with 30 sperm whales stranding in five countries within six weeks. All sperm whales were immature males. Groups were stratified by size, with the smaller animals stranding in the Netherlands, and the largest in England. The majority (n = 27) of the stranded animals were necropsied and/ or sampled, allowing for an international and comprehensive investigation into this mortality event. The animals were in fair to good nutritional condition and, aside from the pathologies caused by stranding, did not exhibit significant evidence of disease or trauma. Infectious agents were found, including various parasite species, several bacterial and fungal pathogens and a novel alphaherpesvirus. In nine of the sperm whales a variety of marine litter was found. However, none of these findings were considered to have been the primary cause of the stranding event. Potential anthropogenic and environmental factors that may have caused the sperm whales to enter the North Sea were assessed. Once sperm whales enter the North Sea and head south, the water becomes progressively shallower (<40 m), making this region a global hotspot for sperm whale strandings. We conclude that the reasons for sperm whales to enter the southern North Sea are the result of complex interactions of extrinsic environmental factors. As such, these large mortality events seldom have a single ultimate cause and it is only through multidisciplinary, collaborative approaches that potentially multifactorial large-scale stranding events can be effectively investigated.

Evaluating potential biomarkers of health and performance in veal calves
Marcato, Francesca ; Brand, Henry van den; Kemp, Bas ; Reenen, Kees van - \ 2018
Frontiers in Veterinary Science 5 (2018)JUN. - ISSN 2297-1769
Biomarkers - Challenges - Diseases - Health - Stress - Veal calves

Veal calves undergo many challenges in the early stages of their life. Such challenges, including mixing procedures and transportation of calves to the veal farm, may have a negative influence on growth rate, feed intake, metabolism, immunity and disease susceptibility of calves. As a consequence, many hematological, physiological, metabolic and immunological parameters of stressed calves might be altered on arrival at the veal farm. Some of these response variables might be useful as biomarkers of performance of calves at the veal farm as they might provide information about an ongoing disease process, or may predict future diseases. Biomarkers might be helpful to group and manage calves in different risk categories after arrival. By adopting treatment decisions and protocols on a risk-group or individual basis, it would be possible to improve animal health and reduce both disease incidence and antibiotic use. Moreover, the use of biomarkers might be an economically feasible approach as some of them do not need invasive techniques and others can be measured in blood already taken during routine checks. Previous literature mainly assessed the physiological responses of calves to transportation. However, information on the link between on-farm arrival data and future health and performance of veal calves is limited. This review, therefore, examined a wide range of papers and aimed to identify potential biomarkers of future health and performance.

Site-specific aftercare completion criteria for sustainable landfilling in the Netherlands : Geochemical modelling and sensitivity analysis
Dijkstra, Joris J. ; Zomeren, André van; Brand, Ellen ; Comans, Rob N.J. - \ 2018
Waste Management 75 (2018). - ISSN 0956-053X - p. 407 - 414.
Aftercare completion - Environmental protection criteria - Geochemical modelling - Risk assessment - Sensitivity analysis - Sustainable landfill management
A novel, regulatory accepted approach is developed that enables competent authorities to decide whether landfill aftercare can be reduced or terminated. Our previous paper (Brand et al., Waste Management 2016, 56, 255–261, https://doi.org//10.1016/j.wasman.2016.07.038) outlines the general approach, that consists of a 10-year treatment phase (e.g., aeration, leachate recirculation), in combination with site-specific Environmental Protection Criteria (EPC) for contaminant concentrations in the landfill leachate after treatment. The current paper presents the unique modelling approach by which the site-specific EPC are derived. The modelling approach is based on the use of mechanistic multi-surface geochemical models covering the main sorption processes in soils underneath the landfills, and is composed of widely-accepted surface complexation models in combination with published “generic” parameter sets. This approach enables the consideration of the main site-specific soil properties that influence the attenuation of emitted contaminants. In addition, the sensitivity of the EPC is shown for variation of the main physicochemical-assumptions and policy-based decisions. Site-specific soil properties have been found to substantially determine the EPC and include soil-pH, dissolved organic matter, and iron-(hydr)oxide content. Apart from the sorption capacity of the local soil, EPC also depend strongly on the assumed dilution with local groundwater in the saturated zone. An important policy-related decision that influences the calculated EPC is the assessment period during which the groundwater is protected. The transparent setup of the approach using geochemical modelling, the explicit consideration of site-specific properties and the achieved regulatory acceptance may also stimulate application to landfills in other countries.
Vuur in het heidelandschap, branden als beheermethode
Castrop, E. ; Roomer, R. ; Hoftijzer, M. ; Stoof, C.R. - \ 2018
Wageningen : Nature Today
Recent werd in Breda een stuk heide van 50 bij 40 meter afgebrand met als doel natuurbeheer, het doel was hier om de heide te verjongen. Dit klinkt gevaarlijk, maar deze gecontroleerde branden zijn niet te vergelijken met ongecontroleerde natuurbranden. Bij een beheerbrand wordt rekening gehouden met het weer voorafgaand aan de brand en het gebeurt alleen onder optimale omstandigheden.
Groei en productie van Corsicaanse den in Nederland
Jansen, J.J. ; Oosterbaan, A. ; Mohren, G.M.J. ; Ouden, J. den - \ 2018
Wageningen : Wageningen University & Research (FEM Groei en Productie rapport 2018-6) - 109
In the Netherlands, growth and yield research on Corsican pine was done from 1925 to 1990. This includes studies by Becking and by the Dorschkamp/IBN research institute. Together with the permanent sample plots from the timber prognosis system HOSP, all this comprises a dataset of 193 plots with 869 recordings. For the development of top height htop over age (t), Jansen & Hilde-brand’s model with asymptote and 3 additional parameters fitted best. As site index, top height at 50 year (h50) was chosen. The diameter development up to stand height of 7 m was best described with a Gompertz function based on htop, the density after refinements (NR), and h50. From a stand height of 7 m and up, the basal area increment (iG) was best described by a power function based on htop, t, and the stand density index of Hart (S %). For S % > 22.4 the basal area increment drops with increas-ing S %. The year of recording in relation with Brunchorstia dieback was also included in the model. The effect of thinning on diameter after thinning was modelled with a modified La Bastide-Faber model. With all models together, a stand projection model was constructed, which follows the meas-ured stand development reasonably well. The model was used to construct yield tables with seven site classes and six thinning intensities.
Objectively measured physical activity and sedentary time are associated with cardiometabolic risk factors in adults with prediabetes : The PREVIEW study
Swindell, Nils ; Mackintosh, Kelly ; Mcnarry, Melitta ; Stephens, Jeffrey W. ; Sluik, Diewertje ; Fogelholm, Mikael ; Drummen, Mathijs ; Macdonald, Ian ; Martinez, J.A. ; Handjieva-Darlenska, Teodora ; Poppitt, Sally D. ; Brand-Miller, Jennie ; Larsen, Thomas M. ; Raben, Anne ; Stratton, Gareth - \ 2018
Diabetes Care 41 (2018)3. - ISSN 0149-5992 - p. 562 - 569.
OBJECTIVE The aim of the present cross-sectional study was to examine the association among physical activity (PA), sedentary time (ST), and cardiometabolic risk in adults with prediabetes. RESEARCH DESIGN AND METHODS Participants (n = 2,326; 25-70 years old, 67% female) from eight countries, with a BMI >25 kg · m22 and impaired fasting glucose (5.6-6.9 mmol · L21) or impaired glucose tolerance (7.8-11.0 mmol · L21 at 2 h), participated. Seven-day accelerometry objectively assessed PA levels and ST. RESULTS Multiple linear regression revealed that moderate-To-vigorous PA (MVPA) was negatively associated withHOMAof insulin resistance (HOMA-IR) (standardizedb =20.078 [95% CI20.128,20.027]), waist circumference (WC) (b =20.177 [20.122,20.134]), fasting insulin (b = 20.115 [20.158, 20.072]), 2-h glucose (b = 20.069 [20.112, 20.025]), triglycerides (b = 20.091 [20.138, 20.044]), and CRP (b = 20.086 [20.127, 20.045]). ST was positively associated with HOMA-IR (b = 0.175 [0.114, 0.236]), WC (b = 0.215 [0.026, 0.131]), fasting insulin (b = 0.155 [0.092, 0.219]), triglycerides (b = 0.106 [0.052, 0.16]), CRP (b = 0.106 [0.39, 0.172]), systolic blood pressure (BP) (b = 0.078 [0.026, 0.131]), and diastolic BP (b = 0.106 [0.39, 20.172]). Associations reported between total PA (counts · min21), and all risk factors were comparable or stronger than for MVPA: HOMA-IR (b = 20.151 [20.194, 20.107]), WC (b = 20.179 [20.224, 20.134]), fasting insulin (b = 20.139 [20.183, 20.096]), 2-h glucose (b = 20.088 [20.131, 20.045]), triglycerides (b = 20.117 [20.162, 20.071]), and CRP (b = 20.104 [20.146, 20.062]). CONCLUSIONS In adults with prediabetes, objectively measured PA and ST were associated with cardiometabolic risk markers. Total PA was at least as strongly associated with cardiometabolic risk markers as MVPA, which may imply that the accumulation of total PA over the day is as important as achieving the intensity of MVPA.
Models, More Models, and Then a Lot More
Babur, O. ; Cleophas, L. ; Brand, M. van den; Tekinerdogan, B. ; Aksit, M. - \ 2018
In: Software Technologies: Applications and Foundations. - Cham : Springer International Publishing (Lecture notes in computer science ) - ISBN 9783319747293 - p. 129 - 135.
With increased adoption of Model-Driven Engineering, the number of related artefacts in use, such as models, metamodels and transformations, greatly increases. To confirm this, we present quantitative evidence from both academia — in terms of repositories and datasets — and industry — in terms of large domain-specific language ecosystems. To be able to tackle this dimension of scalability in MDE, we propose to treat the artefacts as data, and apply various techniques — ranging from information retrieval to machine learning — to analyse and manage those artefacts in a holistic, scalable and efficient way.
Making cocoa origin traceable : Fingerprints of chocolates using Flow Infusion - Electro Spray Ionization - Mass Spectrometry
Acierno, Valentina ; Alewijn, Martin ; Zomer, Paul ; Ruth, Saskia M. van - \ 2018
Food Control 85 (2018). - ISSN 0956-7135 - p. 245 - 252.
Chocolate - FI-ESI-MS - Fingerprint - Origin - Traceability
More and more attention is paid to sustainability in the cocoa production. Tools that assist in making sustainable cocoa traceable are therefore welcome. In the present study, the applicability of Flow Infusion-Electrospray Ionization- Mass Spectrometry (FI-ESI-MS) to assess the geographical origin of cocoa beans used to produce chocolate has been tested. Fifty-seven dark chocolates available on the Dutch market were investigated taking into account the geographical origin of the beans: they originated from Africa (15), Asia (11) and South America (31). The FI-ESI-MS generated non-volatile profiles, were subjected to ANOVA selection (21 masses per sample). Those were subsequently used as fingerprints and investigated using chemometric tools to elucidate information on raw material origin. Principal component analysis revealed a remarkable separation between African and Asian bean chocolates. No clear trend was observed for the chocolates manufactured from South American beans. The kNN results confirmed the possibility to separate African and Asian chocolates as well as these two continents together versus the South America. The inability to separate the three continents at the same time and the weak differentiation of South American samples from the others is linked to brand-related factors, such as the formulation and industrial processing. Although diminishing of the influence of the brand-related factors will improve separation, the current approach is an important step in the geographical assessment of the raw material and the traceability of cocoa in its supply chain.
Local amplification of highly pathogenic avian influenza H5N8 viruses in wild birds in the Netherlands, 2016 to 2017
Poen, Marjolein J. ; Bestebroer, Theo M. ; Vuong, Oanh ; Scheuer, Rachel D. ; Jeugd, Henk P. van der; Kleyheeg, Erik ; Eggink, Dirk ; Lexmond, Pascal ; Brand, Judith M.A. van den; Begeman, Lineke ; Vliet, Stefan van der; Müskens, Gerhard J.D.M. ; Majoor, Frank A. ; Koopmans, Marion P.G. ; Kuiken, Thijs ; Fouchier, Ron A.M. - \ 2018
EuroSurveillance 23 (2018)4. - ISSN 1025-496X
Introduction: Highly pathogenic avian influenza (HPAI) viruses of subtype H5N8 were re-introduced into the Netherlands by late 2016, after detections in southeast Asia and Russia. This second H5N8 wave resulted in a large number of outbreaks in poultry farms and the deaths of large numbers of wild birds in multiple European countries. Methods: Here we report on the detection of HPAI H5N8 virus in 57 wild birds of 12 species sampled during active (32/5,167) and passive (25/36) surveillance activities, i.e. in healthy and dead animals respectively, in the Netherlands between 8 November 2016 and 31 March 2017. Moreover, we further investigate the experimental approach of wild bird serology as a contributing tool in HPAI outbreak investigations. Results: In contrast to the first H5N8 wave, local virus amplification with associated wild bird mortality has occurred in the Netherlands in 2016/17, with evidence for occasional gene exchange with low pathogenic avian influenza (LPAI) viruses. Discussion: These apparent differences between outbreaks and the continuing detections of HPAI viruses in Europe are a cause of concern. With the current circulation of zoonotic HPAI and LPAI virus strains in Asia, increased understanding of the drivers responsible for the global spread of Asian poultry viruses via wild birds is needed.
Effects of dietary supplementation of organic minerals, fish oil and hydrolysed collagen on growth performance and tibia characteristics of broiler chickens
Guz, Bahadir ; Molenaar, R. ; Krimpen, M.M. van; Brand, H. van den; Jong, I.C. de; Kemp, B. - \ 2018
In: Abstract of the WIAS Science Day 2018. - - p. 27 - 27.
The sustainable seafood movement is a Governance concert, with the audience playing a key role
Barclay, Kate ; Miller, Alice - \ 2018
Sustainability 10 (2018)1. - ISSN 2071-1050
Corporate social responsibility - Ecolabels - Ethical consumption - Green marketing - Supply chain management - Sustainable seafood
Private standards, including ecolabels, have been posed as a governance solution for the global fisheries crisis. The conventional logic is that ecolabels meet consumer demand for certified "sustainable" seafood, with "good" players rewarded with price premiums or market share and "bad" players punished by reduced sales. Empirically, however, in the markets where ecolabeling has taken hold, retailers and brands-rather than consumers-are demanding sustainable sourcing, to build and protect their reputation. The aim of this paper is to devise a more accurate logic for understanding the sustainable seafood movement, using a qualitative literature review and reflection on our previous research. We find that replacing the consumer-driven logic with a retailer/brand-driven logic does not go far enough in making research into the sustainable seafood movement more useful. Governance is a "concert" and cannot be adequately explained through individual actor groups. We propose a new logic going beyond consumer- or retailer/brand-driven models, and call on researchers to build on the partial pictures given by studies on prices and willingness-to-pay, investigating more fully the motivations of actors in the sustainable seafood movement, and considering audience beyond the direct consumption of the product in question.
Sustainable intensification through rotations with grain legumes in Sub-Saharan Africa : A review
Franke, A.C. ; Brand, G.J. van den; Vanlauwe, B. ; Giller, K.E. - \ 2018
Agriculture, Ecosystems and Environment 261 (2018). - ISSN 0167-8809 - p. 172 - 185.
Biotic factors - Cereals - Nitrogen fixation - Phosphorus - Residual benefits - Smallholder farmers

We conducted a systematic review of literature on the residual effects of grain legumes in cereal-based systems of sub-Saharan Africa (SSA) to quantify the magnitude and variability of rotational effects, to explore the importance of environmental and management factors in determining variability and to evaluate the evidence of the different mechanisms that explain rotational effects. We retrieved 44 unique publications providing 199 observations comparing continuous cereal performance with that of a grain legume-cereal rotation. The overall mean yield increase of 0.49t grainha-1, equal to an increase of 41% of the continuous cereal yield, is highly significant, but the variability in residual effects is large. Effects were more pronounced in southern Africa, the highlands of East Africa and the Guinea savannah, and less in the humid forest/derived savannah of West Africa and the Sudano-Sahelian zone. Maize showed stronger yield responses after a legume than millet and sorghum. Agro-ecological zone and cereal type were however confounded. All grain legume types significantly improved cereal yields, with stronger residual effects observed after soybean and groundnut than after cowpea. Fertiliser N application to cereals reduces the residual effects of legumes, but the response at 60-120kgNha-1 still equalled 0.32tha-1 or 59% of the response when no N is applied. The sustained benefits with large N applications indicate the importance of non-N effects. While mechanisms for improved soil P availability after grain legumes have been studied in some detail, it remains uncertain how important these are in farmers' fields. Grain legumes are unlikely to have a major influence on the availability of nutrients other than N and P, or on soil pH. Beneficial impacts of grain legumes on soil organic matter content can occur if legumes contribute to a greater overall cropping productivity, but studies generally report no such impacts. Evidence of impacts of grain legumes on weeds is limited to striga. Studies on the impacts on nematode pressure in cereals are inconclusive, probably because legumes act as a host for some of the key nematode genera that harm maize. The impact on the pressure of other pests and diseases in cereals is probably important, but evidence on this from SSA is lacking. Future research on N2-fixation by grain legumes and residual N benefits should focus on explaining the wide variability observed among sites. There is a clear need for more detailed mechanistic studies to assess the occurrence and relevance of non-N effects of grain legumes, particularly in relation to common pests and diseases in cereals.

Authenticity and the Contradictions of the “Ecotourism Script” : Global Marketing and Local Politics in Ghana
Büscher, Bram ; Bremer, Renée van den; Fletcher, Robert ; Koot, Stasja - \ 2017
Critical Arts 31 (2017)4. - ISSN 0256-0046 - p. 37 - 52.
authenticity - development - ecotourism - Ghana - marketing - politics
Tourism in Ghana has been developing rapidly over the last decade. By marketing over a dozen “community ecotourism” sites, particularly around monkey and forest sanctuaries, Ghana hopes to attract travellers to spend money in the country and so aid local development and protect natural resources. This paper analyses this trend, outlining several contradictions in the country’s national branding of “authenticity” in ecotourism and how this takes local shape in the case of the Tafi-Atome monkey sanctuary in Eastern Ghana. We propose that actors on different levels in Ghana appear to market and brand ecotourism according to a “script” that directs and influences local ecotourism practices in ways that obscure these contradictions and thereby enable continuation of and belief in the script. We conclude that this “ecotourism script” is central to the promotion and implementation of ecotourism in general, and needed to maintain the belief that the activity is an important conservation and development panacea.
Dose-Dependent Increase of Triglyceride Levels in Human HepaRG Liver Cells Exposed to the Fungicide Cyproconazole
Peijnenburg, A.A.C.M. ; Rijkers, Deborah ; Stoopen, G.M. - \ 2017
Cyproconazole is a triazole antifungal which has been associated with
the development of hepatic steatosis, also referred to as fatty liver
disease, in rodents. An Adverse Outcome Pathway (AOP) for steatosis has
recently been proposed and one important Key Event (KE) in this AOP
concerns the accumulation of triglycerides. The present work is part of
a larger effort that aims to explore the usefulness of the human liver cell
line HepaRG as an in vitro system to address various KEs in the steatosis
AOP and to test foodborne chemicals and chemical mixtures for steatogenic
properties. In this study, HepaRG cells were exposed for 24 and
72 hours to increasing, non-cytotoxic, concentrations of cyproconazole.
Upon exposure, cells were lysed by sonication and extracted with a
mixture of iso-octane and ethylacetate (75:25) to specifically extract
triglycerides. For further analysis of the fatty acid composition of the
triglycerides, extracts were treated with sodium methoxide and BF3 to
generate fatty acid methyl esters (FAMEs). Triglycerides and FAMEs were
analysed using Gas Chromatography with Flame Ionization Detection
(GC-FID). The results indicated that cyproconazole treatment of HepaRG
cells results in a dose-dependent accumulation of triglycerides. The
effect was strongest after 72 hours of exposure. Accumulation was most
pronounced for C50, C52, and C54 triglycerides containing mainly C16
and C18 fatty acids. This outcome underlines the value of the HepaRG
cell line as a model system for studying toxicant-induced liver steatosis.
Acknowledgement: This work has been funded by the EU project EuroMix
(Grant Agreement 633172; www.euromixproject.eu) and the Dutch Ministry
of Economic Affairs.
Models, More Models, and Then A Lot More
Babur, O. ; Cleophas, L. ; Brand, M. van den; Tekinerdogan, B. ; Aksit, M. - \ 2017
- 2 p.
With increased adoption of Model-Driven Engineering, the number of related artefacts in use, such as models, greatly increase. To be able to tackle this dimension of scalability in MDE, we propose to treat the artefacts as data, and apply
various techniques ranging from information retrieval to machine learning to analyse and manage them in a scalable and efficient way.
A meta-analysis of effects of post-hatch food and water deprivation on development, performance and welfare of chickens
Jong, I.C. de; Riel, J.W. van; Bracke, M.B.M. ; Brand, H. van den - \ 2017
PLoS One 12 (2017)12. - ISSN 1932-6203 - 20 p.
Chicken - Body weight - Yolk Sac - Gastrointestinal tract - animal performance - poultry - jejunum - meta-analysis
A ‘meta-analysis’ was performed to determine effects of post-hatch food and water deprivation (PHFWD) on chicken development, performance and welfare (including health). Two types of meta-analysis were performed on peer-reviewed scientific publications: a quantitative ‘meta-analysis’ (MA) and a qualitative analysis (QA). Previously reported effects of PHFWD were quantified in the MA, for variables related to performance, mortality and relative yolk sac weight. The QA counted the number of studies reporting (non-)significant effects when five or more records were available in the data set (i.e. relative heart, liver and pancreas weight; plasma T3, T4 and glucose concentrations; relative duodenum, jejunum and ileum weight; duodenum, jejunum and ileum length; and villus height and crypt depth in duodenum, jejunum and ileum). MA results indicated that 24 hours of PHFWD (i.e. ≥12–36 hours) or more resulted in significantly lower body weights compared to early-fed chickens up to six weeks of age. Body weights and food intake were more reduced as durations of PHFWD (24, 48, 72, ≥84 hours) increased. Feed conversion rate increased in chickens up to 21 and 42 days of age after ≥84 hours PHFWD in comparison with chickens fed earlier. Total mortality at day 42 was higher in chickens after 48 hours PHFWD compared to early fed chickens or chickens after 24 hours PHFWD. First week mortality was higher in chickens after ≥84 hours PHFWD than in early fed chickens. The MA for relative yolk sac weight was inconclusive for PHFWD. The QA for plasma T3, T4 and glucose concentrations indicated mainly short-term decreases in T3 and glucose in PHFWD chickens compared to early fed chickens, and no effects of PHFWD on T4 concentrations. Relative weights of liver, pancreas and heart were lower after PHFWD, but only in the first week of life. A retarded development of gut segments (duodenum, jejunum and ileum) was found in the first week of life, measured as shorter, lower relative weight, and lower villus height and crypt depth. It is concluded that 48 hours (≥36–60 hours) PHFWD leads to lower body weights and higher total mortality in chickens up to six weeks of age, the latter suggesting compromised chicken welfare, but effects of PHFWD on organ development and physiological status appear to be mainly short-term.
“The influence of incubation upon subsequent broiler performance”
Brand, Henry van den - \ 2017
Check title to add to marked list
<< previous | next >>

Show 20 50 100 records per page

 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.