Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Nutrimetabolomics: An Integrative Action for Metabolomic Analyses in Human Nutritional Studies
Ulaszewska, Marynka M. ; Weinert, Christoph H. ; Trimigno, Alessia ; Portmann, Reto ; Andres Lacueva, Cristina ; Badertscher, René ; Brennan, Lorraine ; Brunius, Carl ; Bub, Achim ; Capozzi, Francesco ; Cialiè Rosso, Marta ; Cordero, Chiara E. ; Daniel, Hannelore ; Durand, Stéphanie ; Egert, Bjoern ; Ferrario, Paola G. ; Feskens, Edith J.M. ; Franceschi, Pietro ; Garcia-Aloy, Mar ; Giacomoni, Franck ; Giesbertz, Pieter ; González-Domínguez, Raúl ; Hanhineva, Kati ; Hemeryck, Lieselot Y. ; Kopka, Joachim ; Kulling, Sabine E. ; Llorach, Rafael ; Manach, Claudine ; Mattivi, Fulvio ; Migné, Carole ; Münger, Linda H. ; Ott, Beate ; Picone, Gianfranco ; Pimentel, Grégory ; Pujos-Guillot, Estelle ; Riccadonna, Samantha ; Rist, Manuela J. ; Rombouts, Caroline ; Rubert, Josep ; Skurk, Thomas ; Sri Harsha, Pedapati S.C. ; Meulebroek, Lieven Van; Vanhaecke, Lynn ; Vázquez-Fresno, Rosa ; Wishart, David ; Vergères, Guy - \ 2018
Molecular Nutrition & Food Research 63 (2018)1. - ISSN 1613-4125
GC–MS - LC–MS - metabolomics - NMR - nutrition
The life sciences are currently being transformed by an unprecedented wave of developments in molecular analysis, which include important advances in instrumental analysis as well as biocomputing. In light of the central role played by metabolism in nutrition, metabolomics is rapidly being established as a key analytical tool in human nutritional studies. Consequently, an increasing number of nutritionists integrate metabolomics into their study designs. Within this dynamic landscape, the potential of nutritional metabolomics (nutrimetabolomics) to be translated into a science, which can impact on health policies, still needs to be realized. A key element to reach this goal is the ability of the research community to join, to collectively make the best use of the potential offered by nutritional metabolomics. This article, therefore, provides a methodological description of nutritional metabolomics that reflects on the state-of-the-art techniques used in the laboratories of the Food Biomarker Alliance (funded by the European Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL)) as well as points of reflections to harmonize this field. It is not intended to be exhaustive but rather to present a pragmatic guidance on metabolomic methodologies, providing readers with useful “tips and tricks” along the analytical workflow.
Quantitative NMR assessment of polysaccharides in complex food matrices
Velzen, E.J.J. van; Dauwan, S. ; Roo, N. de; Grün, C.H. ; Westphal, Y. ; Duynhoven, J.P.M. van - \ 2015
In: Magnetic Resonance in Food Science: Defining food by magnetic resonance / Capozzi, F., Laghi, L., Belton, P.S., Royal Society of Chemistry - ISBN 9781782620310 - p. 39 - 48.
Polysaccharides are a critical component of many food stuffs owing to their stable rheological properties. Food polysaccharides show great structural diversity. These characteristics, together with strong matrix interactions, complicate the quantitative assessment of polysaccharides in complex product formulations. Most current analytical approaches rely on qualitative identification of polysaccharides and quantification based on monosaccharide analysis. NMR for example has typically been applied for qualitative purposes but recently progress has been made in semi-quantification. However employing this technique for the absolute quantitative assessment polysaccharides is less straightforward. This is primarily due to broadened and overlapping lineshapes, which compromise NMR signal integration. In this chapter the authors describe the hybrid NMR approach that they have developed in response to this problem for quantifying polysaccharides in food products. They demonstrate that through combining information on identified polysaccharides and monosaccharide composition it is possible to achieve absolute quantification of polysaccharides in full product formulations in the w/w% range with 10% precision.
19F Labeled polyion micelles as diffusional nanoprobes
Kort, D.W. de; Hoeben, F.J.M. ; Janssen, H.M. ; Bourouina, N. ; Kleijn, J.M. ; Duynhoven, J.P.M. van; As, H. van - \ 2014
In: Magnetic Resonance in Food Science: Defining Food by Magnetic Resonance / Capozzi, F., Laghi, L., Belton, P.S., RSC Publishing - ISBN 9781782622741 - p. 109 - 119.
In modern food manufacturing, there is a strong drive to find alternative formulations in which ingredients are replaced by alternatives derived from a sustainable source. However structure-function relationships remain poorly understood. In order to better understand these relationships, new measurement methods are required to quantify structural properties. At the sub-micron level, powerful microscopy does not allow for reliable quantification of structural features and microscopy methods are invasive and require careful image analysis in order to quantify structural features. For the sub-micron structural characterization of biopolymer hydrogels quantitative nanoprobe diffusometry is emerging as a powerful method to complement knowledge obtained by microscopy. In this study, the authors have used PFG NMR diffusometry to measure diffusion of labeled 19F-C3Ms in a heterogeneous gel model system. In this model system, multi-modal diffusion of dendrimer nanoparticles was observed. They assess the merits of 19F NMR diffusometry for characterization of heterogeneous gels, and compare this to the results obtained by 1H NMR diffusometry.
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