Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Editorial: Identification and incidence of iris yellow spot virus, a new pathogen in onion and leek in Greece
Chatzivassiliou, E.K. ; Giavachtsia, V. ; Hassani-Mehraban, A. ; Hoedjes, K. ; Peters, D. - \ 2009
Plant Disease 93 (2009)7. - ISSN 0191-2917 - p. 761 - 761.
Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) is an emerging and serious pathogen affecting several Allium spp. worldwide (2). The virus causes straw-colored, chlorotic or necrotic lesions that coalesce, occasionally resulting in an extensive necrosis on onion (A. cepa L.) leaves. From February to June 2008, 530 onion and 439 leek (A. porum L.) leaf samples showing a variety of lesions were collected from different areas of Greece. All plants sampled were infested with Thrips tabaci Lindeman, the sole thrips species identified as the vector of this virus. Samples were analyzed by double-antibody sandwich (DAS)-ELISA using polyclonal antibodies against the N protein of IYSV (Laboratory of Virology, Wageningen Agricultural University). A higher percentage of onion samples than leek samples were infected, with IYSV detected in 36, 44, 23.7, 61.7, 10, 55, 15.3, and 9.4% of onion samples from the prefectures of Evros, Heraklion, Kavala, Magnissia, Pella, Rodopi, Thessaloniki, and Viotia, respectively, and in 5, 0, 0, 9.3, and 13% of leek samples from Evros, Heraklion, Magnissia, Pella, and Thessaloniki, respectively. No leek samples were tested from Kavala, Rodopi, and Viotia. Sap extracts from some positive samples were mechanically inoculated onto Nicotiana benthamiana leaves, and infected plants developed typical IYSV symptoms and were positive in DAS-ELISA, confirming transmission from the field samples. Viral RNA was extracted from ELISA-positive onion and leek samples and an ~800-bp amplicon was obtained from both hosts by reverse-transcription (RT)-PCR and N-gene primers derived from IYSV (IY1: 5'-CCCGAGGATCCATGGCTACCGTTAGGG-3' and IY2: 5'-CCCGAGGATCCAAATTAATTATATCTATCTTTCTTGG-3') (1). These amplicons were cloned and sequenced (GenBank Accession No. FJ785835) and nucleotide sequence comparisons showed a 98 to 99% identity with a Dutch isolate of IYSV (GenBank Accession No AF001387). The virus was transmitted among onion seedlings in the laboratory using a leek population of T. tabaci. Infected seedlings, as determined by DAS-ELISA, developed symptoms similar to those observed in the field samples. To our knowledge, this is the first report of IYSV in Greece; however, the virus seems already to be very well established.
Occurrence of Tomato spotted wilt virus in Stevia rebaudiana and Solanum tuberosum in Northern Greece
Chatzivassiliou, E.K. ; Peters, D. ; Lolas, P. - \ 2007
Plant Disease 91 (2007)9. - ISSN 0191-2917 - p. 1205 - 1205.
frankliniella-occidentalis
Tomato spotted wilt virus (TSWV) (genus Tospovirus, family Bunyaviridae) was first reported in Greece during 1972 (3) and currently is widespread in the central and northern part of the country infecting several cultivated and wild plant species (1,2). In June 2006, virus-like symptoms similar to those caused by TSWV were observed on sweet honey leaf (Stevia rebaudiana (Bertoni) Bertoni, [synonym Eupatorium rebaudianum Bertoni], family Asteraceae) plants in an experimental field in the prefecture of Kilkis and on potato (Solanum tuberosum, family Solanaceae) plants growing close to tobacco in the prefecture of Kozani in northern Greece. Diseased S. rebaudiana plants expressed chlorotic and necrotic rings and line patterns on systemically infected leaves and occasionally a general chlorosis or dwarfing of the plant. Potato plants expressed only necrotic rings or lesions and a mild plant dwarfing. Seven percent of the S. rebaudiana plants, but only 0.1% of the potato plants, were showing symptoms. TSWV was detected by ELISA using polyclonal antisera in all symptomatic plants of both species, but those plants tested negative for Potato Y virus (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Potato leafroll virus (PLRV); all viruses known to occur in these areas. Sap from symptomatic plants was used to mechanically inoculate Petunia × hybrida cv. Blue magic, Nicotiana tabacum cvs. Samsun NN and Basmas, N. benthamiana, N. glutinosa, N. rustica, Datura stramonium, and Physalis floridana. Sap from both S. rebaudiana and potato produced typical symptoms of TSWV on the diagnostic test plants; all Nicotiana species and cultivars, as well as D. stramonium and P. floridana, reacted with chlorotic and necrotic lesions on the inoculated leaves followed by systemic mosaic and mottling of the leaves. None of the isolates produced systemic infection on P. × hybrida. Adult thrips, identified as Thrips tabaci, were collected from infected plants of both hosts in the field and determined to be infected with TSWV by the petunia leaf disc method (4). Symptoms observed in the field on both hosts where reproduced in the laboratory after infestation of healthy plants with TSWV-infected individuals from a tobacco population of T. tabaci. To our knowledge, this is the first official report of a TSWV infection in S. rebaudiana. Potato is a known host for TSWV, however, to our knowledge, this is the first natural infection detected in Greece.
The role of weeds in the spread of Tomato spotted wilt virus by thrips tabaci (Thysanoptera: Thripidae) in tobacco crops
Chatzivassiliou, E.K. ; Peters, D. ; Katis, N.I. - \ 2007
Journal of Phytopathology 155 (2007)11-12. - ISSN 0931-1785 - p. 699 - 705.
western flower thrips - frankliniella-occidentalis thysanoptera - insect vector - host-range - transmission - tospovirus - epidemiology - populations - disease - hawaii
Oviposition of Thrips tabaci, larval development and their potential to acquire Tomato spotted wilt virus (TSWV) from infected Amaranthus retroflexus, Datura stramonium, Lactuca serriola, Solanum nigrum and Sonchus oleraceus plants and the ability of the adults to transmit this virus to these weeds and tobacco (Nicotiana tabacum cv. Basmas) were studied. When a T. tabaci female was given an oviposition period of 4¿days, an average of 21 larvae were produced on leaves of D. stramonium, 17.5 on S. nigrum, 16.3 on L. serriola and 14.3 on S. oleraceus. Significantly higher numbers of larvae were found on tobacco (29.5), and lower numbers on A. retroflexus (1.9). In a choice test, when females were placed onto leaves of these weed species and tobacco in a Petri dish, D. stramonium was preferred over tobacco. Equal numbers of larvae emerged on leaves of tobacco, of L. serriola and of S. nigrum. Oviposition was lower on A. retroflexus and S. oleraceus in this test. After the transfer of newborn 24¿h old larvae to leaf discs of tobacco or to one of the weeds 87, 84, 82, 71, 64 and 17% became pupa on tobacco, D. stramonium, L. serriola, S. nigrum, A. retroflexus or S. oleraceus respectively. After acquisition of virus by newborn-24¿h old larvae from L. serriola, D. stramonium, S. nigrum and A. retroflexus 69.5, 51.4, 32.6 and 22% of the adults became transmitters. No transmission could be recorded on S. oleraceus due to a high larval mortality. Males appeared to be more efficient transmitters than females. Tobacco was more susceptible to TSWV than petunia and the weed species, while among weeds, S. nigrum was the most and A. retroflexus the least susceptible species.
The efficiency by which thrips tabaci populations transmit tomato spotted wilt virus depends on their host preference and reproductive strategy
Chatzivassiliou, E.K. ; Peters, D. ; Katis, N.I. - \ 2002
Phytopathology 92 (2002). - ISSN 0031-949X - p. 603 - 609.
Weeds in greenhouses and tobacco fields are differentially infected by tomato spotted wilt virus and infested by its vector species
Chatzivassiliou, E.K. ; Bourbourakas, I. ; Drossos, E. ; Eleftherohorinos, I. ; Jenser, G. ; Peters, D. ; Katis, N.I. - \ 2001
Plant Disease 85 (2001)1. - ISSN 0191-2917 - p. 40 - 46.
Transmission of tomato spotted wilt tospovirus by Thrips tabaci populations originating from leek
Chatzivassiliou, E.K. ; Nagata, T. ; Katis, N.I. ; Peters, D. - \ 1999
Plant Pathology 48 (1999). - ISSN 0032-0862 - p. 700 - 706.
The transmission of tomato spotted wilt tospovirus (TSWV) by Thrips tabaci collected from leek was studied using the petunia local-lesion leaf-disc assay. After an acquisition-access period of 72 h given to newborn larvae up to 8 h old, the efficiency of transmission by adults was determined in three inoculation-access periods of 48 h. This efficiency varied for six T. tabaci populations from 0.7 to 11.6␒n experiments using the Greek TSWV isolate GR-04. Males were more efficient transmitters than females (19 out of 176 versus five out of 494). Frankliniella occidentalis transmitted the same virus with a higher efficiency (34.8Ž The transmission rate differed also among TSWV isolates, as shown in tests with four T. tabaci using two isolates. The virus was more efficiently acquired from infected leaf material of Datura stramonium than from that of Emilia sonchifolia. Plants of the latter species were more susceptible than Nicotiana tabacum in thrips transmission tests.
A closterovirus (family: Closteroviridae) isolated from tobacco crops in Northern Greece (Macedonia)
Chatzivassiliou, E.K. ; Katis, N.I. ; Tchomguia, M. ; Peters, D. - \ 1999
Plant Disease 83 (1999). - ISSN 0191-2917 - p. 198 - 198.
Transmission of Tomato Spotted Wilt Tospovirus by Thrips tabaci populations and determination of the median latent period
Chatzivassiliou, E.K. ; Katis, N.I. ; Peters, D. - \ 1999
In: VIIth International Plant Virus Epidemiology Symposium : Plant Virus Epidemiology : Current status and future prospects : 11 - 16 April 1999, Aguadulce (Almeria), Spain : book of abstracts. - [S.l.] : [s.n.], 1999 - p. 42 - 42.
Transmission of tomato spotted wilt virus (TSWV) by Thrips Tabaci grown on tobacco or non tobacco hosts.
Chatzivassiliou, E.K. ; Katis, E.K. ; Peters, D. - \ 1998
In: 4th International Symposium on Tospoviruses and Thrips in Floral and Vegetable crops, Wageningen, The Netherlands - p. 59 - 62.
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