Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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A collaborative evaluation of LC-MS/MS based methods for BMAA analysis : Soluble bound BMAA found to be an important fraction
Faassen, Els ; Antoniou, Maria G. ; Beekman-Lukassen, Wendy ; Blahova, Lucie ; Chernova, Ekaterina ; Christophoridis, Christophoros ; Combes, Audrey ; Edwards, Christine ; Fastner, Jutta ; Harmsen, Joop ; Hiskia, Anastasia ; Ilag, Leopold L. ; Kaloudis, Triantafyllos ; Lopicic, Srdjan ; Lurling, Miguel ; Mazur-Marzec, Hanna ; Meriluoto, Jussi ; Porojan, Cristina ; Viner-Mozzini, Yehudit ; Zguna, Nadezda - \ 2016
Marine Drugs 14 (2016)3. - ISSN 1660-3397
6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) - Cycad - Daphnia magna - Hydrophilic interaction liquid chromatography (HILIC) - Internal standard - Liquid chromatography-tandem mass spectrometry (LC-MS/MS) - N-(2-aminoethyl) glycine (AEG) - Phytoplankton - Seafood - α,γ-diaminobutyric acid (DAB) - β-N-methylamino-L-alanine (BMAA)

Exposure to β-N-methylamino-L-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D3BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D3BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (

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