Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Validation of biomarkers of food intake-critical assessment of candidate biomarkers
Dragsted, Lars O. ; Gao, Qinfeng ; Scalbert, Augustin ; Vergères, Guy ; Kolehmainen, Marjukka ; Manach, Claudine ; Brennan, Lorraine ; Afman, L.A. ; Wishart, David S. ; Lacueva, Cristina Andres ; Garcia-Aloy, Mar ; Verhagen, H. ; Feskens, E.J.M. ; Feskens, E.J.M. ; Praticò, Giulia - \ 2018
Genes & Nutrition 13 (2018). - ISSN 1555-8932
Biomarkers of food intake (BFIs) are a promising tool for limiting misclassification in nutrition research where more subjective dietary assessment instruments are used. They may also be used to assess compliance to dietary guidelines or to a dietary intervention. Biomarkers therefore hold promise for direct and objective measurement of food intake. However, the number of comprehensively validated biomarkers of food intake is limited to just a few. Many new candidate biomarkers emerge from metabolic profiling studies and from advances in food chemistry. Furthermore, candidate food intake biomarkers may also be identified based on extensive literature reviews such as described in the guidelines for Biomarker of Food Intake Reviews (BFIRev). To systematically and critically assess the validity of candidate biomarkers of food intake, it is necessary to outline and streamline an optimal and reproducible validation process. A consensus-based procedure was used to provide and evaluate a set of the most important criteria for systematic validation of BFIs. As a result, a validation procedure was developed including eight criteria, plausibility, dose-response, time-response, robustness, reliability, stability, analytical performance, and inter-laboratory reproducibility. The validation has a dual purpose: (1) to estimate the current level of validation of candidate biomarkers of food intake based on an objective and systematic approach and (2) to pinpoint which additional studies are needed to provide full validation of each candidate biomarker of food intake. This position paper on biomarker of food intake validation outlines the second step of the BFIRev procedure but may also be used as such for validation of new candidate biomarkers identified, e.g., in food metabolomic studies.
Guidelines for Biomarker of Food Intake Reviews (BFIRev) : How to conduct an extensive literature search for biomarker of food intake discovery
Praticò, Giulia ; Gao, Qian ; Scalbert, Augustin ; Vergères, Guy ; Kolehmainen, Marjukka ; Manach, Claudine ; Brennan, Lorraine ; Pedapati, Sri Harsha ; Afman, Lydia A. ; Wishart, David S. ; Vázquez-Fresno, Rosa ; Lacueva, Cristina Andres ; Garcia-Aloy, Mar ; Verhagen, Hans ; Feskens, Edith J.M. ; Dragsted, Lars O. - \ 2018
Genes & Nutrition 13 (2018)1. - ISSN 1555-8932
Biomarkers - Food exposure markers - Literature search methodology - Metabolomics - Systematic review
Identification of new biomarkers of food and nutrient intake has developed fast over the past two decades and could potentially provide important new tools for compliance monitoring and dietary intake assessment in nutrition and health science. In recent years, metabolomics has played an important role in identifying a large number of putative biomarkers of food intake (BFIs). However, the large body of scientific literature on potential BFIs outside the metabolomics area should also be taken into account. In particular, we believe that extensive literature reviews should be conducted and that the quality of all suggested biomarkers should be systematically evaluated. In order to cover the literature on BFIs in the most appropriate and consistent manner, there is a need for appropriate guidelines on this topic. These guidelines should build upon guidelines in related areas of science while targeting the special needs of biomarker methodology. This document provides a guideline for conducting an extensive literature search on BFIs, which will provide the basis to systematically validate BFIs. This procedure will help to prioritize future work on the identification of new potential biomarkers and on validating these as well as other biomarker candidates, thereby providing better tools for future studies in nutrition and health.
A scheme for a flexible classification of dietary and health biomarkers
Gao, Qian ; Praticò, G. ; Scalbert, A. ; Vergères, Guy ; Kolehmainen, M. ; Manach, Claudine ; Brennan, L. ; Afman, L.A. ; Wishart, D.S. ; Andres-Lacueva, Cristina ; Garcia-Aloy, M. ; Verhagen, H. ; Feskens, E.J.M. ; Dragsted, L.O. - \ 2017
Genes & Nutrition 12 (2017). - ISSN 1555-8932
Biomarkers are an efficient means to examine intakes or exposures and their biological effects and to assess system susceptibility. Aided by novel profiling technologies, the biomarker research field is undergoing rapid development and new putative biomarkers are continuously emerging in the scientific literature. However, the existing concepts for classification of biomarkers in the dietary and health area may be ambiguous, leading to uncertainty about their application. In order to better understand the potential of biomarkers and to communicate their use and application, it is imperative to have a solid scheme for biomarker classification that will provide a well-defined ontology for the field. In this manuscript, we provide an improved scheme for biomarker classification based on their intended use rather than the technology or outcomes (six subclasses are suggested: food compound intake biomarkers (FCIBs), food or food component intake biomarkers (FIBs), dietary pattern biomarkers (DPBs), food compound status biomarkers (FCSBs), effect biomarkers, physiological or health state biomarkers). The application of this scheme is described in detail for the dietary and health area and is compared with previous biomarker classification for this field of research.
Dietary and health biomarkers-time for an update
Dragsted, L.O. ; Gao Qizian, ; Praticò, G. ; Manach, Claudine ; Wishart, D.S. ; Scalbert, A. ; Feskens, E.J.M. - \ 2017
Genes & Nutrition 12 (2017)24. - ISSN 1555-8932 - 7 p.
In the dietary and health research area, biomarkers are extensively used for multiple purposes. These include biomarkers of dietary intake and nutrient status, biomarkers used to measure the biological effects of specific dietary components, and biomarkers to assess the effects of diet on health. The implementation of biomarkers in nutritional research will be important to improve measurements of dietary intake, exposure to specific dietary components, and of compliance to dietary interventions. Biomarkers could also help with improved characterization of nutritional status in study volunteers and to provide much mechanistic insight into the effects of food components and diets. Although hundreds of papers in nutrition are published annually, there is no current ontology for the area, no generally accepted classification terminology for biomarkers in nutrition and health, no systematic validation scheme for these biomarker classes, and no recent systematic review of all proposed biomarkers for food intake. While advanced databases exist for the human and food metabolomes, additional tools are needed to curate and evaluate current data on dietary and health biomarkers. The Food Biomarkers Alliance (FoodBAll) under the Joint Programming Initiative—A Healthy Diet for a Healthy Life (JPI-HDHL)—is aimed at meeting some of these challenges, identifying new dietary biomarkers, and producing new databases and review papers on biomarkers for nutritional research. This current paper outlines the needs and serves as an introduction to this thematic issue of Genes & Nutrition on dietary and health biomarkers.
Host-related factors explaining interindividual variability of carotenoid bioavailability and tissue concentrations in humans
Bohn, Torsten ; Desmarchelier, Charles ; Dragsted, Lars O. ; Nielsen, Charlotte S. ; Stahl, Wilhelm ; Rühl, Ralph ; Keijer, Jaap ; Borel, Patrick - \ 2017
Molecular Nutrition & Food Research 61 (2017)6. - ISSN 1613-4125
Absorption - Biodistribution - Genetic polymorphisms - Intestine - Macula lutea
Carotenoid dietary intake and their endogenous levels have been associated with a decreased risk of several chronic diseases. There are indications that carotenoid bioavailability depends, in addition to the food matrix, on host factors. These include diseases (e.g. colitis), life-style habits (e.g. smoking), gender and age, as well as genetic variations including single nucleotide polymorphisms that govern carotenoid metabolism. These are expected to explain interindividual differences that contribute to carotenoid uptake, distribution, metabolism and excretion, and therefore possibly also their association with disease risk. For instance, digestion enzymes fostering micellization (PNLIP, CES), expression of uptake/efflux transporters (SR-BI, CD36, NPC1L1), cleavage enzymes (BCO1/2), intracellular transporters (FABP2), secretion into chylomicrons (APOB, MTTP), carotenoid metabolism in the blood and liver (LPL, APO C/E, LDLR), and distribution to target tissues such as adipose tissue or macula (GSTP1, StARD3) depend on the activity of these proteins. In addition, human microbiota, e.g. via altering bile-acid concentrations, may play a role in carotenoid bioavailability. In order to comprehend individual, variable responses to these compounds, an improved knowledge on intra-/interindividual factors determining carotenoid bioavailability, including tissue distribution, is required. Here, we highlight the current knowledge on factors that may explain such intra-/interindividual differences.
First successful reduction of clinical allergenicity of food by genetic modification: Mal d 1 silenced apples cause fewer allergy symptoms than the wild-type cultivar
Dubois, A.E.J. ; Pagliarani, G. ; Brouwer, R.M. ; Kollen, B.J. ; Dragsted, L.O. ; Eriksen, F.D. ; Callesen, O. ; Gilissen, L.J.W.J. ; Krens, F.A. ; Visser, R.G.F. ; Smulders, M.J.M. ; Vlieg-Boerstra, B.J. ; Flokstra-de Blok, B.J. ; Weg, W.E. van de - \ 2015
Allergy 70 (2015)11. - ISSN 0105-4538 - p. 1406 - 1412.
Background Genetic modification of allergenic foods such as apple has the potential to reduce their clinical allergenicity, but this has never been studied by oral challenges in allergic individuals. Methods We performed oral food challenges in 21 apple-allergic individuals with Elstar apples which had undergone gene silencing of the major allergen of apple, Mal d 1, by RNA interference. Downregulation of Mal d 1 gene expression in the apples was verified by qRT-PCR. Clinical responses to the genetically modified apples were compared to those seen with the wild-type Elstar using a visual analogue scale (VAS). Results Gene silencing produced two genetically modified apple lines expressing Mal d 1.02 and other Mal d 1 gene mRNA levels which were extensively downregulated, that is only 0.1–16.4% (e-DR1) and 0.2–9.9% (e-DR2) of those of the wild-type Elstar, respectively. Challenges with these downregulated apple lines produced significantly less intense maximal symptoms to the first dose (Vmax1) than with Elstar (Vmax1 Elstar 3.0 mm vs 0.0 mm for e-DR1, P = 0.017 and 0.0 mm for e-DR2, P = 0.043), as well as significantly less intense mean symptoms per dose (meanV/d) than with Elstar (meanV/d Elstar 2.2 mm vs 0.2 mm for e-DR1, P = 0.017 and 0.0 mm for e-DR2, P = 0.043). Only one subject (5%) remained symptom-free when challenged with the Elstar apple, whereas 43% did so with e-DR1 and 63% with e-DR2. Conclusion These data show that mRNA silencing of Mal d 1 results in a marked reduction of Mal d 1 gene expression in the fruit and reduction of symptoms when these apples are ingested by allergic subjects. Approximately half of the subjects developed no symptoms whatsoever, and virtually all subjects wished to consume the apple again in the future.
The role of red processed meat in colorectal cancer development a perspective
Oostindjer, M. ; Alexander, J. ; Amdam, G.V. ; Andersen, G. ; Bryan, N.S. ; Chen, D. ; Corpet, D.E. ; Smet, S. de; Dragsted, L.O. ; Haug, A. ; Karlsson, A.H. ; Kleter, G.A. ; Kok, E.J. ; Kulseng, B. ; Milkowski, A.L. ; Martin, R.J. ; Pajari, A.M. ; Paulsen, J.E. ; Pickova, J. ; Rudi, K. ; Sodring, M. ; Weed, D.L. ; Egelandsdal, B. - \ 2014
Meat Science 97 (2014)4. - ISSN 0309-1740 - p. 583 - 596.
mucin-depleted foci - aberrant crypt foci - endothelium-dependent vasodilation - familial adenomatous polyposis - colonic epithelial-cells - n-3 fatty-acids - dna-damage - intestinal tumorigenesis - microtubule stability - potential mechanisms
This paper is based on a workshop held in Oslo, Norway in November 2013, in which experts discussed how to reach consensus on the healthiness of red and processed meat. Recent nutritional recommendations include reducing intake of red and processed meat to reduce cancer risk, in particular colorectal cancer (CRC). Epidemiological and mechanistic data on associations between red and processed meat intake and CRC are inconsistent and underlying mechanisms are unclear. There is a need for further studies on differences between white and red meat, between processed and whole red meat and between different types of processed meats, as potential health risks may not be the same for all products. Better biomarkers of meat intake and of cancer occurrence and updated food composition databases are required for future studies. Modifying meat composition via animal feeding and breeding, improving meat processing by alternative methods such as adding phytochemicals and improving our diets in general are strategies that need to be followed up.
Challenges of molecular nutrition research 6: the nutritional phenotype database to store, share and evaluate nutritional systems biology studies
Ommen, B. van; Bouwman, J.H. ; Dragsted, L.O. ; Drevon, C.A. ; Elliott, R. ; Groot, P.J. de; Kaput, J. ; Mathers, J.C. ; Müller, M.R. ; Pepping, F. ; Saito, J. ; Scalbert, A. ; Radonjic, M. ; Rocca-Serra, P. ; Travis, A. ; Wopereis, S. ; Evelo, C. - \ 2010
Genes & Nutrition 5 (2010)3. - ISSN 1555-8932 - p. 189 - 203.
gene-expression - metabolic phenotypes - association - framework - services - network - complex - health - diet
The challenge of modern nutrition and health research is to identify food-based strategies promoting life-long optimal health and well-being. This research is complex because it exploits a multitude of bioactive compounds acting on an extensive network of interacting processes. Whereas nutrition research can profit enormously from the revolution in ‘omics’ technologies, it has discipline-specific requirements for analytical and bioinformatic procedures. In addition to measurements of the parameters of interest (measures of health), extensive description of the subjects of study and foods or diets consumed is central for describing the nutritional phenotype. We propose and pursue an infrastructural activity of constructing the “Nutritional Phenotype database” (dbNP). When fully developed, dbNP will be a research and collaboration tool and a publicly available data and knowledge repository. Creation and implementation of the dbNP will maximize benefits to the research community by enabling integration and interrogation of data from multiple studies, from different research groups, different countries and different-omics levels. The dbNP is designed to facilitate storage of biologically relevant, pre-processed-omics data, as well as study descriptive and study participant phenotype data. It is also important to enable the combination of this information at different levels (e.g. to facilitate linkage of data describing participant phenotype, genotype and food intake with information on study design and-omics measurements, and to combine all of this with existing knowledge). The biological information stored in the database (i.e. genetics, transcriptomics, proteomics, biomarkers, metabolomics, functional assays, food intake and food composition) is tailored to nutrition research and embedded in an environment of standard procedures and protocols, annotations, modular data-basing, networking and integrated bioinformatics. The dbNP is an evolving enterprise, which is only sustainable if it is accepted and adopted by the wider nutrition and health research community as an open source, pre-competitive and publicly available resource where many partners both can contribute and profit from its developments. We introduce the Nutrigenomics Organisation (NuGO, http://www.nugo.org) as a membership association responsible for establishing and curating the dbNP. Within NuGO, all efforts related to dbNP (i.e. usage, coordination, integration, facilitation and maintenance) will be directed towards a sustainable and federated infrastructure
Bioactive compounds: Definition and assessment of activity
Biesalski, H.K. ; Dragsted, L.O. ; Elmadfa, I. ; Grossklaus, R. ; Müller, M.R. ; Schrenk, D. ; Walter, P. ; Weber, P. - \ 2009
Nutrition 25 (2009)11-12. - ISSN 0899-9007 - p. 1202 - 1205.
risk factor - disease
Biomarkers and their role in evaluating efficacy and safety were the topic of the 23rd Hohenheim Consensus Meeting at the University of Hohenheim in Stuttgart. Scientists who had published and reviewed scientific and regulatory papers on the topic were invited, among them basic researchers, toxicologists, clinicians, and nutritionists. The participants were presented with 11 questions (in bold font), which were discussed and answered (in italic font) at the workshop, with the aim of summarizing the current state of knowledge on the subject. The explicatory text accompanying the short answers was produced and agreed on after the conference and was backed up by corresponding references
Development of hypo-allergenic apples: silencing of the major allergen Mal d 1 gene in 'Elstar' apple and the effect of grafting
Krath, B.N. ; Eriksen, F.D. ; Pedersen, B.H. ; Gilissen, L.J.W.J. ; Weg, W.E. van de; Dragsted, L.O. - \ 2009
Journal of Horticultural Science and Biotechnology 84 (2009)6 Isafruit Suppl. - ISSN 1462-0316 - p. 52 - 57.
controlled food challenge - birch pollen allergen - malus-domestica - double-blind - expression - protein - cloning - bet-v-1 - fruit
Many people who are allergic to birch pollen are also allergic to apple fruit, due to cross- allergenicity. Since apples are the most extensively consumed fruit in Europe, it is highly relevant to develop a hypo-allergenic apple. Apples with significantly reduced levels of the allergen, Mal d 1, may allow many apple allergics to eat them without an allergic reaction. We are currently collaborating to develop a hypo-allergenic apple within the European Integrated Research Project, ISAFRUIT (www.isafruit.org). Hypo-allergenic apple plants (Malus × domestica Borkh., ‘Elstar’) with decreased levels of Mal d 1 mRNA were produced by RNA interference (RNAi) technology. Ten genetically modified (GM) apple lines were selected. In vitro plantlets were first transferred to a greenhouse, then grafted onto wild-type M.9 rootstock to promote the development of fruit-producing trees. Levels of Mal d 1 gene silencing were measured repeatedly by quantitative real-time PCR. Compared to leaf samples from wild-type ‘Elstar’, two GM lines showed modest levels of gene silencing (up to 250-fold), whereas the other eight GM lines were significantly silenced (up to10,000-fold) in Mal d 1 gene expression. These levels of silencing were unaffected by grafting, and have been stable over more than 3 years, and throughout all developmental stages.
Bioactive compounds: Safety and efficacy (Consensus Meeting - Part II)
Biesalski, H.K. ; Dragsted, L.O. ; Elmadfa, I. ; Grossklaus, R. ; Müller, M.R. ; Schrenk, D. ; Walter, P. ; Weber, P. - \ 2009
Nutrition 25 (2009)11-12. - ISSN 0899-9007 - p. 1206 - 1211.
single-nucleotide polymorphisms - human genome - receptors - sequence - disease - diet
The efficacy and safety of bioactive compounds depend on a few known and unknown parameters. What is a physiologic dose and how can that dose be defined in cases of bioactive compounds with a poor knowledge of supply and distribution? What safety sets are needed? How can individual aspects such as polymorphisms or differences in absorption be considered? A group of experts tried to answer these and related questions during the 23rd Hohenheim Consensus Meeting at the University of Hohenheim in Stuttgart. (C) 2009 Elsevier Inc. All rights reserved.
ß-Carotene does not change markers of enzymatic and nonenzymatic antioxidant activity in human blood
Castenmiller, J.J.M. ; Lauridsen, S.T. ; Dragsted, L.O. ; Hof, K.H. van 't; Linssen, J.P.H. ; West, C.E. - \ 1999
The Journal of Nutrition 129 (1999). - ISSN 0022-3166 - p. 2162 - 2169.
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