- S. Christensen-Dalsgaard (2)
- D.B. Diep (1)
- Jens Eiken (1)
- K. Fangel (3)
- J.U. Fangel (1)
- J.A. Frank (1)
- William G.T. Willats (1)
- A. Götsch (1)
- Jesper Harholt (1)
- T. Haughdahl (1)
- D. Herzke (3)
- A.A. Koelmans (3)
- M. Langset (3)
- T. Nøst (1)
- M. Oostindjer (1)
- P.B. Pope (1)
- Aafje Sierksma (1)
- C. Souza Da Silva (1)
- Jonatan U. Fangel (1)
- O.C.O. Umu (1)
- W.G.T. Willats (1)
Tracking polysaccharides through the brewing process
Fangel, Jonatan U. ; Eiken, Jens ; Sierksma, Aafje ; Schols, Henk A. ; Willats, William G.T. ; Harholt, Jesper - \ 2018
Carbohydrate Polymers 196 (2018). - ISSN 0144-8617 - p. 465 - 473.
Beer - Enzymes - Glycan arrays - Malt - Polysaccharides - Wort
Brewing is a highly complex stepwise process that starts with a mashing step during which starch is gelatinized and converted into oligo- and/or monosaccharides by enzymes and heat. The starch is mostly degraded and utilised during the fermentation process, but grains and hops both contain additional soluble and insoluble complex polysaccharides within their cell walls that persist and can have beneficial or detrimental effects on the brewing process. Previous studies have mostly been restricted to analysing the grain and/or malt prior to entering the brewing process, but here we track the fates of polysaccharides during the entire brewing process. To do this, we utilised a novel approach based on carbohydrate microarray technology. We demonstrate the successful application of this technology to brewing science and show how it can be utilised to obtain an unprecedented level of knowledge about the underlying molecular mechanisms at work.
Negligible Impact of Ingested Microplastics on Tissue Concentrations of Persistent Organic Pollutants in Northern Fulmars off Coastal Norway
Herzke, D. ; Anker-Nilssen, T. ; Haughdahl, T. ; Nøst, T. ; Götsch, A. ; Christensen-Dalsgaard, S. ; Langset, M. ; Fangel, K. ; Koelmans, A.A. - \ 2016
Environmental Science and Technology 50 (2016)4. - ISSN 0013-936X - p. 1924 - 1933.
The northern fulmar (Fulmarus glacialis) is defined as an indicator species of plastic pollution by the Oslo-Paris Convention for the North-East Atlantic, but few data exist for fulmars from Norway. Moreover, the relationship between uptake of plastic and pollutants in seabirds is poorly understood. We analyzed samples of fulmars from Norwegian waters and compared the POP concentrations in their liver and muscle tissue with the corresponding concentrations in the loads of ingested plastic in their stomachs, grouped as “no”, “medium” (0.01–0.21 g; 1–14 pieces of plastic), or “high” (0.11–0.59 g; 15–106 pieces of plastic). POP concentrations in the plastic did not differ significantly between the high and medium plastic ingestion group for sumPCBs, sumDDTs, and sumPBDEs. By combining correlations among POP concentrations, differences in tissue concentrations of POPs between plastic ingestion subgroups, fugacity calculations, and bioaccumulation modeling, we showed that plastic is more likely to act as a passive sampler than as a vector of POPs, thus reflecting the POP profiles of simultaneously ingested prey.
|Modeling the relationship between POPs in ingested plastic debris and in tissue of Norwegian Northern Fulmars
Herzke, D. ; Koelmans, A.A. ; Langset, M. ; Christensen-Dalsgaard, S. ; Fangel, K. - \ 2015
|Relationship between Persistent organic pollutant concentrations on ingested plastic debris and tissue concentrations in Norwegian Northern Fulmars
Herzke, D. ; Koelmans, A.A. ; Langset, M. ; Christensen, S. ; Fangel, K. ; Anker-Nilssen, T. - \ 2015
Resistant starch diet induces change in the swine microbiome and a predominance of beneficial bacterial populations
Umu, O.C.O. ; Frank, J.A. ; Fangel, J.U. ; Oostindjer, M. ; Souza Da Silva, C. ; Bolhuis, J.E. ; Bosch, G. ; Willats, W.G.T. ; Pope, P.B. ; Diep, D.B. - \ 2015
Microbiome 3 (2015). - ISSN 2049-2618
Background Dietary fibers contribute to health and physiology primarily via the fermentative actions of the host’s gut microbiome. Physicochemical properties such as solubility, fermentability, viscosity, and gel-forming ability differ among fiber types and are known to affect metabolism. However, few studies have focused on how they influence the gut microbiome and how these interactions influence host health. The aim of this study is to investigate how the gut microbiome of growing pigs responds to diets containing gel-forming alginate and fermentable resistant starch and to predict important interactions and functional changes within the microbiota. Results Nine growing pigs (3-month-old), divided into three groups, were fed with either a control, alginate-, or resistant starch-containing diet (CON, ALG, or RS), and fecal samples were collected over a 12-week period. SSU (small subunit) rDNA amplicon sequencing data was annotated to assess the gut microbiome, whereas comprehensive microarray polymer profiling (CoMPP) of digested material was employed to evaluate feed degradation. Gut microbiome structure variation was greatest in pigs fed with resistant starch, where notable changes included the decrease in alpha diversity and increase in relative abundance of Lachnospiraceae- and Ruminococcus-affiliated phylotypes. Imputed function was predicted to vary significantly in pigs fed with resistant starch and to a much lesser extent with alginate; however, the key pathways involving degradation of starch and other plant polysaccharides were predicted to be unaffected. The change in relative abundance levels of basal dietary components (plant cell wall polysaccharides and proteins) over time was also consistent irrespective of diet; however, correlations between the dietary components and phylotypes varied considerably in the different diets. Conclusions Resistant starch-containing diet exhibited the strongest structural variation compared to the alginate-containing diet. This variation gave rise to a microbiome that contains phylotypes affiliated with metabolically reputable taxonomic lineages. Despite the significant microbiome structural shifts that occurred from resistant starch-containing diet, functional redundancy is seemingly apparent with respect to the microbiome’s capacity to degrade starch and other dietary polysaccharides, one of the key stages in digestion.