Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins
Mantzouki, E. ; Campbell, J. ; Loon, E. van; Visser, P. ; Konstantinou, I. ; Antoniou, M. ; Giuliani, G. ; Machado-Vieira, D. ; Gurjão de Oliveira, A. ; Maronić, D.Š. ; Stević, F. ; Pfeiffer, T.Ž. ; Vucelić, I.B. ; Žutinić, P. ; Udovič, M.G. ; Plenković-Moraj, A. ; Tsiarta, N. ; Bláha, L. ; Geriš, R. ; Fránková, M. ; Christoffersen, K.S. ; Warming, T.P. ; Feldmann, T. ; Laas, A. ; Panksep, K. ; Tuvikene, L. ; Kangro, K. ; Häggqvist, K. ; Salmi, P. ; Arvola, L. ; Fastner, J. ; Straile, D. ; Rothhaupt, K.O. ; Fonvielle, J. ; Grossart, H.P. ; Avagianos, C. ; Kaloudis, T. ; Triantis, T. ; Zervou, S.K. ; Hiskia, A. ; Gkelis, S. ; Panou, M. ; McCarthy, V. ; Senerpont Domis, L.N. de; Seelen, L. ; Verstijnen, Y. ; Lürling, M. ; Maliaka, V. ; Faassen, E.J. - \ 2018
Scientific Data 5 (2018). - ISSN 2052-4463 - 13 p.
Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment.
Mitigating GHG emissions from EU agriculture : what difference does the policy make?
Feldmann, T. ; Perez Dominguez, I. ; Witzke, H.P. ; Oudendag, D.A. - \ 2012
- 13 p.
Selenium speciation in different organs of African catfish (Clarias gariepinus) enriched through a selenium-enriched garlic based diet
Pedrero, Z. ; Murillo, S. ; Camara, C. ; Schram, E. ; Luten, J.B. ; Feldmann, I. ; Jakubowski, N. ; Madrid, Y. - \ 2011
Journal of Analytical Atomic Spectrometry 26 (2011)1. - ISSN 0267-9477 - p. 116 - 125.
hplc-icp-ms - enzymatic probe sonication - isotope-dilution analysis - plasma-mass spectrometry - food-chain selenium - se-speciation - sample preparation - brassica-juncea - human health - brazil nuts
Speciation of Se in fish is needed to elucidate the metabolism of this element in living organisms in the marine environment. In this paper, selenium concentration and its species distribution in several organs and tissues (liver, gills, kidney, muscle and gastrointestinal tract) of African catfish fed with a selenium-enriched garlic based diet was studied. The intention of this paper is focused on both the investigation of selenium distribution in the soluble protein fraction and the detection of selenoaminoacids. Thus, two different procedures have been developed. In the first procedure, screening of selenium in proteins in the Tris-buffer soluble fraction of different tissues was carried out by size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) and by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and electroblotting onto membranes. For the amino acid analysis, several sample treatments for Se-species extraction, based on enzymatic hydrolysis, were compared. The best results were obtained for incubation at controlled temperature. Application of several sample treatments in conjunction with different chromatographic techniques (reverse phase, anion exchange and ion exchange/size exclusion) was crucial to unambiguous Se-species identification. In Se-enriched African catfish a noticeable increase in the content of selenium in different organs was observed, except for the liver, where the Se level remained unaltered. The kidney was the Se-target organ in animals fed with enriched Se food. Selenomethionine (SeMet) was the main Se species identified in fillet extracts, whereas the presence of selenocysteine (SeCys) was detected in the liver and both SeMet and SeCys were present in the kidney.
Screening of selenium containing proteins in the Tris-buffer soluble fraction of African catfish (Clarias gariepinus) fillets by laser ablation-ICP-MS after SDS-PAGE and electroblotting onto membranes
Pedrero, Z. ; Madrid, Y. ; Camara, C. ; Schram, E. ; Luten, J.B. ; Feldmann, I. ; Waentig, L. ; Hayen, H. ; Jakubowski, N. - \ 2009
Journal of Analytical Atomic Spectrometry 24 (2009)6. - ISSN 0267-9477 - p. 775 - 784.
plasma-mass spectrometry - 2-dimensional gel-electrophoresis - liquid-chromatography - isotope-dilution - cell disruption - size-exclusion - rich yeast - speciation - identification - tissues
The anti-carcinogenic properties of selenium against certain types of cancer when present in organic forms justify the increasing interest in development of selenium fortified food. In this particular study, African catfish (Clarias gariepinus) were fed with a Se-enriched diet in order to enhance the selenium concentration in the fish fillet up to 0.85 ± 0.06 mg Se kg-1 (0.26 ± 0.02 mg Se kg-1 in control). Selenium distribution in proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was studied for better understanding of the metabolism of this element in the organism. Soluble proteins (Tris buffer) were investigated by conventional application of a glass homogenizer in comparison to application of ultrasound probe sonication, and the latter was convincing due to higher protein extraction efficiency and the ease and speed of application. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was successfully applied for screening of selenium in proteins in fillets of African catfish separated by one-dimensional SDS-PAGE and after electroblotting onto nitrocellulose membranes, with a total Se amount below 0.4 ng loaded into a well. Selenium was detected in more than 11 protein spots. In order to improve the protein separation of one-dimensional SDS-PAGE, pre-fractionation of the Tris buffer soluble protein extract by size exclusion was carried out. Tryptic digestion of the Se-containing bands was performed for protein identification by nano-HPLC coupled to electrospray-MS/MS. A database search revealed several proteins which are located in muscle tissue. However, the very low Se concentration circumvents the detection of selenium containing amino acids in the tryptic peptides by electrospray-MS/MS
The determination of ecological quality in shallow lakes - a tested system (EcoFrame) for implementation of the European Water Framework Directive
Moss, B. ; Stephen, D. ; Alvarez, C. ; Becares, E. ; Bunt, W. van de; Collings, S.E. ; Donk, E. van; Eyto, E. de; Feldmann, T. ; Fernandez-Alaez, C. ; Fernandez-Alaez, M. ; Franken, R.J.M. ; Carcia-Criado, F. ; Gross, E.M. ; Gyllstrom, M. ; Hansson, L. ; Irvine, K. ; Jarvalt, A. ; Jenssen, J.P. ; Jeppesen, E. ; Kairesalo, T. ; Kornijow, R. ; Krause, T. ; Kunnap, H. ; Laas, A. ; Lill, E. ; Lorens, B. ; Luup, H. ; Miracle, M. ; Noges, P. ; Noges, T. ; Nykannen, M. ; Ott, I. ; Peczula, W. ; Peeters, E.T.H.M. ; Phillips, G. ; Romo, S. ; Russell, V. ; Salujoe, J. ; Scheffer, M. ; Siewertsen, K. ; Smal, H. ; Tesch, C. ; Timm, H. ; Tuvikene, L. ; Tonno, I. ; Virro, T. ; Vicente, E. ; Wilson, D. - \ 2003
Aquatic conservation: marine and freshwater ecosystems 13 (2003)6. - ISSN 1052-7613 - p. 507 - 549.
phosphorus transfer-functions - trophic structure - temperate lakes - danish lakes - top-down - land-use - zooplankton - management - phytoplankton - conservation
1. The European Water Framework Directive requires the determination of ecological status in European fresh and saline waters. This is to be through the establishment of a typology of surface water bodies, the determination of reference (high status) conditions in each element (ecotype) of the typology and of lower grades of status (good, moderate, poor and bad) for each ecotype. It then requires classification of the status of the water bodies and their restoration to at least 'good status' in a specified period. 2. Though there are many methods for assessing water quality, none has the scope of that defined in the Directive. The provisions of the Directive require a wide range of variables to be measured and give only general guidance as to how systems of classification should be established. This raises issues of comparability across States and of the costs of making the determinations. 3. Using expert workshops and subsequent field testing, a practicable pan-European typology and classification system has been developed for shallow lakes, which can easily be extended to all lakes. It is parsimonious in its choice of determinands, but based on current limnological understanding and therefore as cost-effective as possible. 4. A core typology is described, which can be expanded easily in particular States to meet local conditions. The core includes 48 ecotypes across the entire European climate gradient and incorporates climate, lake area, geology of the catchment and conductivity. 5. The classification system is founded on a liberal interpretation of Annexes in the Directive and uses variables that are inexpensive to measure and ecologically relevant. The need for taxonomic expertise is minimized. 6. The scheme has been through eight iterations, two of which were tested in the field on tranches of 66 lakes. The final version, Version 8, is offered for operational testing and further refinement by statutory authorities. Copyright (C) 2003 John Wiley Sons, Ltd.
Two dimensional gel analysis of midgut proteins of Anopheles stephensi lines with different susceptibility to Plasmodium falciparum infection
Prevot, G.I. ; Laurent-Winter, C. ; Feldmann, A.M. ; Rodhain, F. ; Bourgouin, C. - \ 1998
Insect Molecular Biology 7 (1998)4. - ISSN 0962-1075 - p. 375 - 383.
Little is known about the composition of the mosquito midgut which plays a central role in the development and subsequent transmission of malaria parasites. As a first step towards the characterization of mosquito midgut molecules involved in the transmission of malaria parasites, we analysed two-dimensional gel electrophoresis patterns of the midgut proteins of sugar-fed and blood-fed Anopheles stephensi lines of different susceptibility to P. falciparum infection. Two lines fully susceptible and one line (Pb3-9A) of reduced susceptibility were used. In the refractory line ookinetes do develop but are only inefficiently transformed into oocysts (Feldmann and Ponnudurai, 1989). The protein profiles of midguts from all sugar-fed mosquito lines were similar. However, after blood feeding, the midgut of the fully susceptible lines contained proteins not found in the midgut of line Pb3-9A. Twenty-nine such proteins were detected and are candidates for involvement in the interaction between the mosquito midgut and P. falciparum.
Genetics of refractoriness to Plasmodium falciparum in the mosquito Anopheles stephensi
Feldmann, A.M. ; Gemert, G.J. van; Vegte-Bolmer, M.C. van de; Jansen, R.C. - \ 1998
Medical and Veterinary Entomology 12 (1998). - ISSN 0269-283X - p. 302 - 312.
Spodoptera exigua multicapsid nucleopolyhedrovirus genetics.
Vlak, J.M. ; Heldens, J.G.M. ; Muñoz, D.M. ; Broer, R. ; Feldmann, A.M. ; Liu, Y. ; Strien, E.A. van; Zuidema, D. - \ 1996
In: Abstract 29th Annual Meeting Society for Invertebrate Pathology, Cordoba, Spain - p. 88 - 88.
Spodoptera exigua multicapsid nucleopolyhedrovirus deletion mutants generated in cell culture lack virulence in vivo.
Heldens, J.G.M. ; Strien, E.A. van; Leisy, D.J. ; Feldmann, A.M. ; Muñoz, D. ; Kulcsar, P. ; Zuidema, D. ; Goldbach, R.W. ; Vlak, J.M. - \ 1996
Journal of General Virology 77 (1996). - ISSN 0022-1317 - p. 3127 - 3134.
Binding of the CrylVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins
Feldmann, F. ; Dullemans, A. ; Waalwijk, C. - \ 1995
Applied and Environmental Microbiology 61 (1995). - ISSN 0099-2240 - p. 2601 - 2605.
A physical and genetic map of the Spodoptera exigua nuclear polyhedrosis virus genome.
Heldens, J.G.M. ; Strien, E.A. van; Feldmann, A.M. ; Mans, R.M.W. ; Zuidema, D. ; Vlak, J.M. - \ 1994
In: 6th Int. Coll. Invertebrate pathology and microbial control, Montpellier, France - p. 221 - 221.
Epicuticular wax and eceriferum mutants.
Lemieux, B. ; Koornneef, M. ; Feldmann, K.A. - \ 1994
In: Arabidopsis / Meyerowitz, E.M., Somerville, C.R., Cold Spring Harbor Laboratory Press - p. 1031 - 1047.
Structural organization of a 17 kb fragment of Spodoptera exigua nuclear polyhedrosis virus.
Strien, E.A. van; Gerrits, H. ; Kulcsar, P. ; Feldmann, A.M. ; Zuidema, D. ; Vlak, J.M. - \ 1993
In: Abstract 12th Ann. Meeting Am. Soc. Virology, Davis, Ca, USA (1993) 48. Ook: Abstr. PhD Summerschool Molecular diversity in pest and disease organisms, WAU - p. 34 - 34.
Eradication of Glossina palpalis palpalis (Robineau-Desvoidy) (Diptera: Glossinidae) from agropastoral land in central Nigeria by means of the sterile insect technique.
Olandunmade, M.A. ; Feldmann, U. ; Takken, W. ; Tenabe, S.O. ; Hamann, H.J. ; Onah, J. ; Dengwat, L. ; Vloed, A.M.V. van der; Gingrich, R.E. - \ 1990
In: Sterile insect technique for tsetse control and eradication Vienna : Int. Atomic Energy Agency - p. 5 - 23.
Karyotype displacement in a laboratory population of the two-spotted spider mite Tetranychus urticae (Koch): Experiments and simulations
Feldmann, A.M. ; Sabelis, M.W. - \ 1981
Genetica 55 (1981). - ISSN 0016-6707 - p. 93 - 110.
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