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The long and winding road leading to the successful introgression of downy mildew resistance into onion
Scholten, O.E. ; Heusden, A.W. van; Khrustaleva, L.I. ; Burger, K. ; Mank, R. ; Antonise, R. ; Harrewijn, J. ; Haecke, W. van; Oost, E.H. ; Peters, R.J. ; Kik, C. - \ 2007
Euphytica 156 (2007)3. - ISSN 0014-2336 - p. 345 - 353.
allium cepa - ziekteresistentie - meeldauw - onderzoek - marker assisted breeding - resistentieveredeling - disease resistance - mildews - research - resistance breeding - allium-cepa l - roylei stearn - peronospora-destructor - subgenus rhizirideum - interspecific cross - linkage - genes - nomenclature - polymorphism - inheritance
Downy mildew resistance originating from Allium roylei Stearn provides a complete resistance to onions and is based on one, dominant gene. Since A. roylei can successfully be hybridized with onion (A. cepa L.), a breeding scheme aimed at the introgression of this gene was initiated ca. 20 years ago. Several setbacks in this programme were encountered, firstly the identified molecular marker linked to the downy mildew resistance locus became increasingly difficult to use and finally lost its discriminating power and secondly the final step, making homozygous introgression lines (ILs), turned out to be more difficult then was hoped. GISH analysis showed that the chromosomal region harbouring the resistance locus was the only remaining piece of A. roylei in the nuclear background of onion and it also confirmed that this region was located on the distal end of chromosome 3. It was hypothesized that some factor present in the remaining A. roylei region was lethal when homozygously present in an onion genetic background. The identification of an individual with a smaller and more distally located introgression fragment and homozygous ILs in its progeny validated this hypothesis. With the help of these nearly isogenic lines four AFLP® markers closely linked to the resistance gene were identified, which can be used for marker-aided selection. The introduction of downy mildew resistance caused by Peronospora destructor into onion is a significant step forward in the development of environmentally-friendly onion cultivars.
Downy mildew resistance originating from Allium roylei Stearn provides a complete resistance to onions and is based on one, dominant gene. Since A. roylei can successfully be hybridized with onion (A. cepa L.), a breeding scheme aimed at the introgression of this gene was initiated ca. 20 years ago. Several setbacks in this programme were encountered, firstly the identified molecular marker linked to the downy mildew resistance locus became increasingly difficult to use and finally lost its discriminating power and secondly the final step, making homozygous introgression lines (ILs), turned out to be more difficult then was hoped. GISH analysis showed that the chromosomal region harbouring the resistance locus was the only remaining piece of A. roylei in the nuclear background of onion and it also confirmed that this region was located on the distal end of chromosome 3. It was hypothesized that some factor present in the remaining A. roylei region was lethal when homozygously present in an onion genetic background. The identification of an individual with a smaller and more distally located introgression fragment and homozygous ILs in its progeny validated this hypothesis. With the help of these nearly isogenic lines four AFLP (R) markers closely linked to the resistance gene were identified, which can be used for marker-aided selection. The introduction of downy mildew resistance caused by Peronospora destructor into onion is a significant step forward in the development of environmentally-friendly onion cultivars.
|European Phenology Network: Nature's Calendar on the move
Vliet, A.J.H. van; Braun, P. ; Brügger, R. ; Bruns, E. ; Clevers, J.G.P.W. ; Estreguil, C. ; Flechsig, M. ; Groot, R.S. de; Grutters, M. ; Harrewijn, J. ; Jeanneret, F. ; Martens, P. ; Menne, B. ; Menzel, A. ; Sparks, T. - \ 2003
Wageningen : Wageningen University - 64 p.
|Natural Terpenoids as Messengers. A multicisciplinary study of their production, biological functions and practical applications
Harrewijn, P. ; Oosten, A.M. van; Piron, P.G.M. - \ 2001
Dordrecht : Kluwer Academic Publishers - ISBN 0792368916 - 452
terpenen - semiochemicals - biosynthese - plantenfysiologie - gezondheid - terpenoids - biosynthesis - plant physiology - health
|CGA 293'343 effects on Myzus persicae: electrical penetration graph studies and effect on non-persistent virus transmission
Harrewijn, P. ; Kogel, W.J. de; Piron, P.G.M. - \ 1998
In: Proceedings of the 1998 Brighton Conference Pests and Diseases (2) - p. 813 - 818.
|Evolution of vascular feeding in aphids: an electrophysiological study
Harrewijn, P. ; Piron, P.G.M. ; Ponsen, M.B. - \ 1998
Entomologia Experimentalis et Applicata 9 (1998). - ISSN 0013-8703 - p. 29 - 34.
Control review of air-borne tulip breaking virus and lily symptomless virus in Lilium in the Netherlands
Asjes, C.J. ; Blom-Barnhoorn, G.J. ; Piron, P.G.M. ; Harrewijn, P. ; Oosten, A.M. van - \ 1998
Acta Horticulturae 432 (1998). - ISSN 0567-7572 - p. 290 - 297.
In this study on the air-borne viruses in Lilium, mainly lily symptomless virus (LSV) and tulip breaking virus (TBV), various factors involved in the efficiency of control are dealt with. The incidence of LSV was reduced very substantially from the old 100␛ate. TBV occurs at fairly low rates. Lily virus X (LVX) and cucumber mosaic virus (CMV) occurred at low level in the last decade. The symptoms of TBV to induce the roguing of diseased plants became less important, if the ELISA-testing of bulbs before bulk propagation by the scaling of bulbs and by tissue culture procedures was effectively applied. The reduction in bulb yield and the relative reduced quality of cut flowers indicate the need for virus control. The virus spread as affected by the elimination of virus source plants strongly became dependent on the ELISA-testing. The speed of spread was generally experienced to be differentially rapid over the season, while a range of c. 25 aphid species were able to transmit LSV and TBV. The chemical control of spread is efficiently done by the routine spraying of mineral oil plus pyrethroid. Terpenoids were not effective. Some effect was observed from a pheromone, plant oil, and -pinene polymer emulsion. Deltamethrin and zetamethrin sprays were most effective. The non-chemical control by the polymer web coverage of crops proved impractical under field conditions. The factors in the chain of control which have a weak impact, e.g., delayed elimination of TBV-diseased plants in the field if the ELISA-bulb testing prior to planting is routinely unapplicable in varieties of L. longiflorium and Oriental hybrids, or strong impact, e.g., testing of bulbs by ELISA of a large part of the assortiment of cultivars, chemical control of virus spread, and rapid propagation procedures, on the efficiency to improve the health situation in the lily culture were indicated.
Distinct feeding behavior between sexes of Frankliniella occidentalis results in higher scar production and lower tospovirus transmission by females.
Wetering, F. van de; Hulshof, J. ; Posthuma, K. ; Harrewijn, P. ; Goldbach, R. ; Peters, D. - \ 1998
Entomologia Experimentalis et Applicata 88 (1998). - ISSN 0013-8703 - p. 9 - 15.
Feeding behavior and scar production of male and female F. occidentalis (Pergande) (Thysanoptera: Thripidae) were studied in relation to transmission of tomato spotted wilt tospovirus (TSWV). Electrical penetration graph (EPG) analysis showed that females feed more frequently and intensively than males. The feeding intensity, reflected by silvery scar production and studied by an image analysis system, demonstrated that females induced more numerous scars than males. At the same time, males transmitted TSWV with a higher efficiency than females, indicating that TSWV transmission and scar production are not positively correlated. Furthermore, males produced significantly more local lesions of TSWV than females. These quantitative differences in scar production and transmission of TSWV can be explained by the lower mobility and higher consumption rate of females. The influence of the sex-ratio on crop damage and virus transmission, and thus to the spread of TSWV, is emphasized.
Pymetrozine, a fast-acting and selective inhibitor of aphid feeding. In-situ studies with electronic monitoring of feeding behaviour
Harrewijn, P. ; Kayser, H. - \ 1997
Pesticide science : a journal of international research and technology on crop protection and pest control 49 (1997). - ISSN 0031-613X - p. 130 - 140.
|The effects of natural terpenoids on behaviour and host plant acceptance of aphids
Harrewijn, R. ; Piron, P.G.M. ; Heuvel, J.F.J.M. van den - \ 1996
In: Book of Abstracts ]SCE 13th Annual Meeting, Praha: 56-57
|Electrically recorded probing behaviour of thrips species on optimal and suboptimal hosts
Harrewijn, P. ; Piron, P.G.M. ; Mollema, C. - \ 1996
Entomologia Experimentalis et Applicata 80 (1996). - ISSN 0013-8703 - p. 43 - 46.
Electrical recording of plant penetration by western flower thrips.
Harrewijn, P. ; Tjallingii, W.F. ; Mollema, C. - \ 1996
Entomologia Experimentalis et Applicata 79 (1996). - ISSN 0013-8703 - p. 345 - 353.
Plant penetration by western flower thrips (Frankliniella occidentalis (Pergande)) was analysed with the electrical penetration graph technique (EPG, DC-system). Thrips attached to a gold wire were included in an electrical circuit to record EPGs when penetrating the plant tissues with their stylets. Three basic EPG waveforms have been distinguished, correlated with stylet penetration into cells, salivation, and ingestion, respectively. The main difference with EPGs of Homoptera is the occurrence of continued separate penetrations that are not necessarily followed by ingestion. Insertion of the stylets causes strong voltage fluctuations in the EPG. We could confirm earlier evidence that penetration of cells and subsequent ingestion of (part of) the protoplast takes less than 20 seconds. Repeated short penetrations can be followed by a continuous feeding pattern during which the stylets are not withdrawn. The same sequence of waveforms is produced on other plant parts such as fruits or pollen grains. The specific waveforms are mainly caused by electromotive force (emf). The emf component was recorded with high resolution and the correlation of waveform details with activities of the cibarial muscle system is discussed.
|Kunststofvlies: alternatieve bescherming kostbare pootgoed
Piron, P.G.M. ; Harrewijn, P. - \ 1995
Aardappelwereld 49 (1995)3. - ISSN 0169-653X - p. 23 - 24.
teelt - cultuurmethoden - insecten - plantenziekten - plantenziekteverwekkende schimmels - plantenplagen - gewasbescherming - plantenvirussen - kunststoffilm - pootaardappelen - cultivation - cultural methods - insects - plant diseases - plant pathogenic fungi - plant pests - plant protection - plant viruses - plastic film - seed potatoes
|Evolution of plant volatile production in insect-plant relationships
Harrewijn, P. ; Minks, A.K. ; Mollema, C. - \ 1995
Chemoecology 516 (1995). - ISSN 0937-7409 - p. 55 - 73.
|Elektronische Registration von Wirtspflanzakzeptationsbenehmen von saugenden Insekten, insbesondere Aphiden und Thripsen. Eidg
Harrewijn, P. - \ 1995
|Resistance of plants (including wild plants) to aphids and thrips
Harrewijn, P. - \ 1995
|Epidemiology of virus transmission by aphids
Harrewijn, P. - \ 1995
|How to make Electrical Penetration Graphs of thrips. Quantification of probing in virus transmission studies
Harrewijn, P. - \ 1995
|Bestrijding van trips met de nadruk op de problemen in de preiteelt
Harrewijn, P. - \ 1995
|Levenswijze en bestrijdingsmogelijkheden van trips, met bijzondere aandacht voor het probleem trips in prei
Harrewijn, P. - \ 1995
In: Het preibedrijf naar 2010. Brochure van DLV Vollegrondsgroenteteelt - p. 78 - 80.
|Plants are better protected after exposure to infested conspecifics
Bruin, J. ; Groot, A.T. ; Sabelis, M.W. ; Dicke, M. - \ 1992
In: Proc.8th Int.Symp.Insect Plant Relationships / Menken, S.B.J., Visser, J.H., Harrewijn, P., Wageningen : - p. 357 - 358.