Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Prospects for harnessing biocide resistance for bioremediation and detoxification
Atashgahi, Siavash ; Sánchez-Andrea, Irene ; Heipieper, Hermann J. ; Meer, Jan R. van der; Stams, Alfons J.M. ; Smidt, Hauke - \ 2018
Science 360 (2018)6390. - ISSN 0036-8075 - p. 743 - 746.
Prokaryotes in natural environments respond rapidly to high concentrations of chemicals and physical stresses. Exposure to anthropogenic toxic substances—such as oil, chlorinated solvents, or antibiotics—favors the evolution of resistant phenotypes, some of which can use contaminants as an exclusive carbon source or as electron donors and acceptors. Microorganisms similarly adapt to extreme pH, metal, or osmotic stress. The metabolic plasticity of prokaryotes can thus be harnessed for bioremediation and can be exploited in a variety of ways, ranging from stimulated natural attenuation to bioaugmentation and from wastewater treatment to habitat restoration.
Energetics and surface properties of Pseudomonas putida DOT-T1E in a two-phase fermentation system with 1-decanol as second phase
Neumann, G. ; Cornelissen, S. ; Breukelen, F.R. van; Hunger, S. ; Lippold, H. ; Loffhagen, N. ; Wick, L.Y. ; Heipieper, H.J. - \ 2006
Applied and Environmental Microbiology 72 (2006)6. - ISSN 0099-2240 - p. 4232 - 4238.
gram-negative bacteria - escherichia-coli - solvent-tolerant - organic-solvents - membrane-vesicles - aeruginosa pao1 - energy-charge - fatty-acids - a-band - growth
The solvent-tolerant strain Pseudomonas putida DOT-TIE was grown in batch fermentations in a 5-liter bioreactor in the presence and absence of 10% (vol/vol) of the organic solvent 1-decanol. The growth behavior and cellular energetics, such as the cellular ATP content and the energy charge, as well as the cell surface hydrophobicity and charge, were measured in cells growing in the presence and absence of 1-decanol. Although the cells growing in the presence of 1-decanol showed an about 10% reduced growth rate and a 48% reduced growth yield, no significant differences were measured either in the ATP and potassium contents or in the energy charge, indicating that the cells adapted completely at the levels of membrane permeability and energetics. Although the bacteria needed additional energy for adaptation to the presence of the solvent, they were able to maintain or activate electron transport phosphorylation, allowing homeostasis of the ATP level and energy charge in the presence of the solvent, at the price of a reduced growth yield. On the other hand, significantly enhanced cell hydrophobicities and more negative cell surface charges were observed in cells grown in the presence of 1-decanol. Both reactions occurred within about 10 min after the addition of the solvent and were significantly different after killing of the cells with toxic concentrations of HgCl2. This adaptation of the surface properties of the bacterium to the presence of solvents seems to be very similar to previously observed reactions on the level of lipopolysaccharides, with which bacteria adapt to environmental stresses, such as heat shock, antibiotics, or low oxygen content. The results give clear physiological indications that the process with P. putida DOT-TIE as the biocatalyst and 1-decanol as the solvent is a stable system for two-phase biotransformations that will allow the production of fine chemicals in economically sound amounts.
Regiospecific effect of 1-octanol on cis-trans isomerization of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12
Heipieper, H.J. ; Waard, P. de; Meer, P. van der; Killian, J.A. ; Isken, S. ; Bont, J.A.M. de; Eggink, G. ; Wolf, F.A. de - \ 2001
Applied Microbiology and Biotechnology 57 (2001). - ISSN 0175-7598 - p. 541 - 547.
The solvent-tolerant bacterium Pseudomonas putida S12, which adapts its membrane lipids to the presence of toxic solvents by a cis to trans isomerization of unsaturated fatty acids, was used to study possible in vivo regiospecificity of the isomerase. Cells were supplemented with linoleic acid (C18:2(9-cis,(12-cis), a fatty acid that cannot be synthesized by this bacterium, but which was incorporated into membrane lipids up to an amount of 15% of total fatty acids. After addition of 1-octanol, which was used as an activator of the cis-trans isomerase, the linoleic acid was converted into the (9-trans,(12-cis isomer, while the (9-cis,(12-trans and (9-trans,(12-trans isomers were not synthesized. Thus, for the first time, regiospecific in vivo formation of novel, mixed cis/trans isomers of dienoic fatty acid chains was observed. The maximal conversion (27-36% of the chains) was obtained at 0.03-0.04% (v/v) octanol, after 2 h. The observed regiospecificity of the enzyme, which is located in the periplasmic space, could be due to penetration of the enzyme to a specific depth in the membrane as well as to specific molecular recognition of the substrate molecules
Ethanol tolerance and membrane fatty acid adaptation in adh multiple and null mutants of Kluyveromyces lactis
Heipieper, H.J. ; Isken, S. ; Saliola, M. - \ 2000
Research in Microbiology 151 (2000). - ISSN 0923-2508 - p. 777 - 784.
Methane oxidation by Dutch grassland and peat soil microflora.
Heipieper, H.J. ; Bont, J.A.M. de - \ 1997
Chemosphere 35 (1997). - ISSN 0045-6535 - p. 3025 - 3037.
Mannitol, a novel compatible solute in Pseudomonas putida S12
Kets, E.P.W. ; Galinski, E.A. ; Wit, M. de; Bont, J.A.M. de; Heipieper, H.J. - \ 1996
Journal of Bacteriology 178 (1996). - ISSN 0021-9193 - p. 6665 - 6670.
Physiological response of Pseudomonas putida S12 subjected to reduced water activity.
Kets, E.P.W. ; Bont, J.A.M. de; Heipieper, H.J. - \ 1996
FEMS Microbiology Letters 139 (1996). - ISSN 0378-1097 - p. 133 - 137.
The effect of osmotic stress, given as decreased water activity (aw), on growth and the accumulation of potassium and the compatible solute betaine by Pseudomonas putida S12 was investigated. Reduced aw was imposed by addition of sodium chloride, sucrose, glycerol or polyethylene glycol to the growth medium. Accumulation of potassium and betaine was established when sodium chloride and sucrose were used to cause osmotic stress. No accumulation of these solutes was found in the presence of glycerol. Addition of polyethylene glycol to the medium strongly decreased the growth rate in comparison with the other osmolytes tested at the corresponding aw. Although polyethylene glycol did decrease the aw, neither potassium nor betaine was accumulated by the cells.
Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12.
Kets, E.P.W. ; Galinski, E.A. ; Wit, M. de; Bont, J.A.M. de; Heipieper, H.J. - \ 1996
Journal of Bacteriology 178 (1996). - ISSN 0021-9193 - p. 6665 - 6670.
Trans-ungesättigte Fettsäuren bei Bakterien - Vorkommen, Synthese und Bedeutung.
Keweloh, H. ; Loffeld, B. ; Heipieper, H.J. - \ 1996
BioSpektrum 2 (1996). - ISSN 0947-0867 - p. 18 - 25.
Trans unsaturated fatty acids in bacteria.
Keweloh, H. ; Heipieper, H.J. - \ 1996
Lipids 31 (1996). - ISSN 0024-4201 - p. 129 - 137.
Effect of environmental factors on the trans/cis ratio of unsaturated fatty acids in Pseudomonas putida S12.
Heipieper, H.J. ; Meulenbeld, G. ; Oirschot, Q. van; Bont, J.A.M. de - \ 1996
Applied and Environmental Microbiology 62 (1996). - ISSN 0099-2240 - p. 2773 - 2777.
Adaptation mechanisms of bacteria to the toxic effects on their membranes by organic pollutants.
Weber, F.J. ; Heipieper, H.J. ; Sikkema, J. ; Isken, S. ; Bont, J.A.M. de - \ 1996
In: UIB-GBF-CSIC-TUB Symp. on Biodegradation of Organic Pollutants. Mallorca - p. 71 - 71.
Bacteria growing in the presence of a second phase of toxic organic solvents.
Isken, S. ; Heipieper, H.J. ; Weber, F.J. ; Sikkema, J. ; Bont, J.A.M. de - \ 1996
In: Abstract 1st Int. Congr. on Extremophiles, Estoril, Portugal - p. 62 - 62.
Effect of environmental stress on the cis/trans isomerization of fatty acids in Pseudomonas putida S12.
Heipieper, H.J. ; Bont, J.A.M. de - \ 1996
In: Abstract NBC 6th Congr. Amsterdam, The Netherlands (1996) PF-13
The integrated grassland project: aims, coherence and site description.
Dasselaar, A. van; Oenema, O. ; Segers, R. ; Leffelaar, P.A. ; Kengen, S.W.M. ; Stams, A.J.M. ; Heipieper, H.J. ; Bont, J.A.M. de - \ 1995
In: Abstract Soc. General Microbiology (UK), Golden Jubilee Meeting. Bath, UK (1995) 64 (P14)
The cis/trans isomerisation of unsaturated fatty acids in Pseudomonas putida: An indicator for the toxicity of organic compounds.
Heipieper, H.J. ; Loffeld, B. ; Keweloh, H. ; Bont, J.A.M. de - \ 1995
In: DECHEMA-Jahrestagung. Wiesbaden, Germany. Abstract book Band 2 - p. 237 - 238.
The cis/trans isomerisation of unsaturated fatty acids in Pseudomonas putida S12: an indicator for environmental stress due to organic compounds.
Heipieper, H.J. ; Loffeld, B. ; Keweloh, H. ; Bont, J.A.M. de - \ 1995
Chemosphere 30 (1995). - ISSN 0045-6535 - p. 1041 - 1051.
The cis/trans isomerisation of unsaturated fatty acids in Pseudomonas putida: use as an indicator for toxicity of organic compounds.
Heipieper, H.J. ; Bont, J.A.M. de - \ 1995
BioSpektrum (1995). - ISSN 0947-0867 - p. 66 - PA069.
The integrated grassland project: methane consumption by indigenous grassland microflora.
Heipieper, H.J. ; Bont, J.A.M. de - \ 1995
In: Abstract Soc. General Microbiology (UK), Golden Jubilee Meeting. Bath, UK (1995) 66 (P17)
The integrated CH4 grassland project: methane consumption by indigenous grassland microflora.
Heipieper, H.J. ; Bont, J.A.M. de - \ 1995
In: Proc. Conf. on Climate change research: evaluation and policy implications, S. Zwerver et al. (eds.). Elsevier Sci. Publ., Amsterdam - p. 581 - 584.
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