Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

  • help
  • print

    Print search results

  • export

    Export search results

  • alert
    We will mail you new results for this query: q=Horecka
Check title to add to marked list
HEx : A heterologous expression platform for the discovery of fungal natural products
Harvey, Colin J.B. ; Tang, Mancheng ; Schlecht, Ulrich ; Horecka, Joe ; Fischer, Curt R. ; Lin, Hsiao Ching ; Li, Jian ; Naughton, Brian ; Cherry, James ; Miranda, Molly ; Li, Yong Fuga ; Chu, Angela M. ; Hennessy, James R. ; Vandova, Gergana A. ; Inglis, Diane ; Aiyar, Raeka S. ; Steinmetz, Lars M. ; Davis, Ronald W. ; Medema, Marnix H. ; Sattely, Elizabeth ; Khosla, Chaitan ; Onge, Robert P.S. ; Tang, Yi ; Hillenmeyer, Maureen E. - \ 2018
Science Advances 4 (2018)4. - ISSN 2375-2548
For decades, fungi have been a source of U.S. Food and Drug Administration-approved natural products such as penicillin, cyclosporine, and the statins. Recent breakthroughs in DNA sequencing suggest that millions of fungal species exist on Earth, with each genome encoding pathways capable of generating as many as dozens of natural products. However, the majority of encoded molecules are difficult or impossible to access because the organisms are uncultivable or the genes are transcriptionally silent. To overcome this bottleneck in natural product discovery, we developed the HEx (Heterologous EXpression) synthetic biology platform for rapid, scalable expression of fungal biosynthetic genes and their encoded metabolites in Saccharomyces cerevisiae. We applied this platform to 41 fungal biosynthetic gene clusters from diverse fungal species from around the world, 22 of which produced detectable compounds. These included novel compounds with unexpected biosynthetic origins, particularly from poorly studied species. This result establishes the HEx platform for rapid discovery of natural products from any fungal species, even those that are uncultivable, and opens the door to discovery of the next generation of natural products.
Check title to add to marked list

Show 20 50 100 records per page

 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.