Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Modeling nutrients in Lake Dianchi (China) and its watershed
Li, Xiaolin ; Janssen, Annette B.G. ; Klein, Jeroen J.M. de; Kroeze, Carolien ; Strokal, Maryna ; Ma, Lin ; Zheng, Yi - \ 2019
Agricultural Water Management 212 (2019). - ISSN 0378-3774 - p. 48 - 59.
Critical loading - MARINA nutrient model - Mining - PCLake ecosystem model - River export of nutrients

Lake Dianchi suffered from severe eutrophication for decades. Past efforts to reduce the eutrophication were not very effective. The objective of this study is to improve our understanding of nitrogen (N) and phosphorus (P) loadings and to analyze to what extent they exceed critical nutrient loadings of Lake Dianchi. To this end, we applied the nutrient MARINA model and the ecosystem model PCLake. Results show that river export of dissolved N and P was high in 2012. About 6 209 ton of total dissolved N (TDN) was exported to the lake (i.e. 23.6 kg ha−1), of which more than two-thirds in the form of dissolved inorganic N. For total dissolved P, this export was about 413 ton (i.e. 1.6 kg ha−1), of which 75% dissolved inorganic P. Urban sewage is a major source of nutrients in rivers in the northern sub-basins. In southern sub-basins, agriculture is an important source of both N and P, while P mining and processing is a major source of dissolved inorganic P. Nutrient inputs to the lake are particularly high from urbanization sub-basins draining into the northern part of the lake (Caohai). Critical nutrient loadings for the northern part of the lake (Caohai) are 0.34 mg P m−2d−1 (3.06 mg N m−2d−1) and for the southern part (Waihai) 0.38 mg P m−2d−1 (3.42 mg N m−2d−1). Actual loadings exceed the critical nutrient loadings by 82 times and 17 times of Caohai and Waihai, respectively. Our study illustrates how linking MARINA with PCLake helped to quantify the causes of lake eutrophication and to identify critical loadings for N and P in the lake. Our study can assist local authorities to formulate management options to reduce nutrient pollution in Lake Dianchi in the future.

Improving the applicability and transparency of land use change modelling : The iCLUE model
Verweij, P. ; Cormont, A. ; Kok, K. ; Eupen, M. van; Janssen, S. ; Roller, J. te; Winter, W. de; Pérez-Soba, M. ; Staritsky, I.G. - \ 2018
Environmental Modelling & Software 108 (2018). - ISSN 1364-8152 - p. 81 - 90.
Land use change - Model - Separation of concerns - Transparency - Usability - User centered design

Human use of land increasingly alters the structure and the functioning of the environment. To ex-ante understand and anticipate these changes there is an increased need for readily available and operational land use change models. One of these models is CLUE, which has been used in many studies all over the world. These studies brought forward operational hurdles, that hamper model application. The overall objective of this paper is to present a new version of the CLUE model, iCLUE, that helps to overcome these hurdles. We describe the technical redevelopment, conceptual innovations, several applications and success factors and critical reflections. iCLUE minimizes manual error-prone actions, enhances ease-of-use, speeds up the operational modelling process and provides data visualisations to empower users to analyse and interpret results.

Data from: Insect pollination is at least as important for marketable crop yield as plant quality in a seed crop
Fijen, T.P.M. ; Scheper, J.A. ; Boom, Timo M. ; Janssen, N. ; Raemakers, Ivo ; Kleijn, D. - \ 2018
crop pollination - crop yield - agricultural management - functional groups - species richness - visitation rate - agro-ecology - structural equation modeling - Allium porrum
The sustainability of agriculture can be improved by integrating management of ecosystem services, such as insect pollination, into farming practices. However, large‐scale adoption of ecosystem services‐based practices in agriculture is lacking, possibly because growers undervalue the benefits of ecosystem services compared to those of conventional management practices. Here we show that, under representative real‐world conditions, pollination and plant quality made similar contributions to marketable seed yield of hybrid leek (Allium porrum). Relative to the median, a 25% improvement of plant quality and pollination increased crop value by an estimated $18 007 and $17 174 ha−1 respectively. Across five crop lines, bumblebees delivered most pollination services, while other wild pollinator groups made less frequent but nevertheless substantial contributions. Honeybees actively managed for pollination services did not make significant contributions. Our results show that wild pollinators are an undervalued agricultural input and managing for enhancing pollinators makes sense economically in high‐revenue insect‐pollinated cropping systems.
Insect pollination is at least as important for marketable crop yield as plant quality in a seed crop
Fijen, Thijs P.M. ; Scheper, Jeroen A. ; Boom, Timo M. ; Janssen, Nicole ; Raemakers, Ivo ; Kleijn, David - \ 2018
Ecology Letters (2018). - ISSN 1461-023X
The sustainability of agriculture can be improved by integrating management of ecosystem services, such as insect pollination, into farming practices. However, large‐scale adoption of ecosystem services‐based practices in agriculture is lacking, possibly because growers undervalue the benefits of ecosystem services compared to those of conventional management practices. Here we show that, under representative real‐world conditions, pollination and plant quality made similar contributions to marketable seed yield of hybrid leek (Allium porrum). Relative to the median, a 25% improvement of plant quality and pollination increased crop value by an estimated $18 007 and $17 174 ha−1 respectively. Across five crop lines, bumblebees delivered most pollination services, while other wild pollinator groups made less frequent but nevertheless substantial contributions. Honeybees actively managed for pollination services did not make significant contributions. Our results show that wild pollinators are an undervalued agricultural input and managing for enhancing pollinators makes sense economically in high‐revenue insect‐pollinated cropping systems.
Scenariostudie natuurperspectief Grevelingenmeer
Tangelder, Marijn ; Wijsman, Jeroen ; Janssen, John ; Nolte, Arno ; Walles, Brenda ; Ysebaert, Tom - \ 2018
Yerseke : Wageningen Marine Research (Wageningen Marine Research rapport C021/18) - 164
The combined and interactive effects of zinc, temperature, and phosphorus on the structure and functioning of a freshwater community
Perre, Dimitri Van de; Roessink, Ivo ; Janssen, Colin R. ; Smolders, Erik ; Laender, Frederik De; Brink, Paul J. van den; Schamphelaere, Karel A.C. De - \ 2018
Environmental Toxicology and Chemistry 37 (2018)9. - ISSN 0730-7268 - p. 2413 - 2427.
Climate change - Community-level effects - Ecotoxicology - Freshwater toxicology - Metal toxicity - Plankton - Zinc

Ecotoxicological studies mainly consist of single-species experiments evaluating the effects of a single stressor. However, under natural conditions aquatic communities are exposed to a mixture of stressors. The present study aimed to identify how the toxicity of zinc (Zn) is affected by increased temperature and increased phosphorus (P) supply and how these interactions vary among species, functional groups, and community structure and function. Aquatic microcosms were subjected to 3 Zn concentrations (background, no Zn added, and 75 and 300 μg Zn/L), 2 temperatures (16–19 and 21–24 °C), and 2 different P additions (low, 0.02, and high, 0.4 mg P L−1 wk−1) for 5 wk using a full factorial design. During the study, consistent interactions between Zn and temperature were only rarely found at the species level (4%), but were frequently found at the functional group level (36%), for community structure (100%) and for community function (100%; such as dissolved organic carbon concentrations and total chlorophyll). The majority of the Zn × temperature interactions were observed at 300 μg Zn/L and generally indicated a smaller effect of Zn at higher temperature. Furthermore, no clear indication was found that high P addition by itself significantly affected the overall effects of Zn on the community at any level of organization. Interestingly, though, 90% of all the Zn × temperature interactions observed at the species, group, and community composition level were found under high P addition. Collectively, the results of our study with the model chemical Zn suggest that temperature and phosphorus loading to freshwater systems should be accounted for in risk assessment, because these factors may modify the effects of chemicals on the structure and functioning of aquatic communities, especially at higher levels of biological organization. Environ Toxicol Chem 2018;37:2413–2427.

Microalgae: from Bio-based Curiosity Towards a Bulk Feedstock
Lam, G.P. 't; Vermuë, M.H. ; Janssen, M. ; Barbosa, M.J. ; Wijffels, R.H. ; Eppink, M.H.M. ; Berg, C. van den - \ 2018
In: Intensification of Biobased Processes / Górak, Andrzej, Stankiewicz, Andrzej, Royal Society of Chemistry (RSC Green Chemistry ) - ISBN 9781782628552 - p. 289 - 302.

This chapter provides a comprehensive overview of the recent developments in microalgae cultivation and downstream processing with a focus on microalgae biorefinery. Microalgae are promising feedstocks for the production of a variety of bulk compounds in the near future. Current process designs typically consist of microalgae cultivation, harvesting, cell disruption and finally a product extraction/fractionation step. For every unit operation, the latest insights are discussed. From this overview, the current state-of-development and future directions towards large scale bulk-chemical production from microalgae are discussed.

Risk assessment of microplastics in the ocean: modelling approach and first conclusions
Everaert, G. ; Cauwenberghe, L. van; Rijcke, M. De; Koelmans, A.A. ; Mees, J. ; Vandegehuchte, Martijn L. ; Janssen, C.R. - \ 2018
Environmental Pollution (2018). - ISSN 0269-7491 - 9 p.
We performed an environmental risk assessment for microplastics (<5 mm) in the marine environment by estimating the order of magnitude of the past, present and future concentrations based on global plastic production data. In 2100, from 9.6 to 48.8 particles m−3 are predicted to float around in the ocean, which is a 50-fold increase compared to the present-day concentrations. From a meta-analysis with effect data available in literature, we derived a safe concentration of 6650 buoyant particles m−3 below which adverse effects are not likely to occur. Our risk assessment (excluding the potential role of microplastics as chemical vectors) suggests that on average, no direct effects of free-floating microplastics in the marine environment are to be expected up to the year 2100. Yet, even today, the safe concentration can be exceeded in sites that are heavily polluted with buoyant microplastics. In the marine benthic compartment between 32 and 144 particles kg−1 dry sediment are predicted to be present in the beach deposition zone. Despite the scarcity of effect data, we expect adverse ecological effects along the coast as of the second half of the 21st century. From then ambient concentrations will start to outrange the safe concentration of sedimented microplastics (i.e. 540 particles kg−1 sediment). Additional ecotoxicological research in which marine species are chronically exposed to realistic environmental microplastic concentration series are urgently needed to verify our findings.
Pre-treatment and digestion of plant proteins - The quinoa case
Opazo Navarrete, Mauricio - \ 2018
University. Promotor(en): Remko Boom, co-promotor(en): Anja Janssen. - Wageningen : Wageningen University - ISBN 9789463437837 - 184
Efficacy of oral compared with intramuscular Vitamin B-12 supplementation after Roux-en-Y gastric bypass : A randomized controlled trial
Schijns, Wendy ; Homan, Jens ; Meer, Leah van der; Janssen, Ignace M. ; Laarhoven, Cees J. van; Berends, Frits J. ; Aarts, Edo O. - \ 2018
American Journal of Clinical Nutrition 108 (2018)1. - ISSN 0002-9165 - p. 6 - 12.
cobalamin - deficiencies - Roux-en-Y gastric bypass - RYGB - supplementation - Vitamin B-12

Background After Roux-en-Y gastric bypass (RYGB), patients often develop a vitamin B-12 deficiency. Objective Our objective was to investigate whether oral supplementation increases and normalizes low vitamin B-12 concentrations (vitamin B-12 > 200 pmol/L) in RYGB patients as compared to intramuscular injections. Design A randomized controlled trial in RYGB patients with subnormal serum B-12 concentrations was performed. One group (IM B-12) received bimonthly intramuscular hydroxocobalamin injections (2000 μg as loading dose and 1000 μg at follow-up) for 6 mo. The second group (oral B-12) received daily doses of oral methylcobalamin (1000 μg). Serum vitamin B-12 was determined at baseline (T0) and at 2 (T1), 4 (T2), and 6 mo (T3) after start of treatment. Concentrations of the secondary markers methylmalonic acid (MMA) and homocysteine (Hcy) were measured at T0 and T3. Results Fifty patients were included and randomized, 27 in IM B-12 and 23 in oral B-12. The median vitamin B-12 concentration at T0 was 175 pmol/L (range: 114-196 pmol/L) for IM B-12 and 167 pmol/L (range: 129-199 pmol/L) for oral B-12. Vitamin B-12 normalized in all individuals, and there was no significant difference in vitamin B-12 between the two groups. MMA and Hcy concentrations decreased significantly after 6 mo within each group (P < 0.001 and P < 0.001 for MMA and P = 0.03 and P = 0.045 for Hcy, respectively). There was no significant difference between the groups at 6 mo for both MMA and Hcy (P = 0.53 and P = 0.79). Conclusion The efficacy of oral vitamin B-12 supplementation was similar to that of hydroxocobalamin injections in the present study. Oral supplementation can be used as an alternative to hydroxocobalamin injections to treat RYGB patients with low values of serum vitamin B-12. This trial was registered at clinicaltrials.gov as NCT02270749.

Altered neural responsivity to food cues in relation to food preferences, but not appetite-related hormone concentrations after RYGB-surgery
Zoon, Harriët F.A. ; Bruijn, Suzanne E.M. de; Smeets, Paul A.M. ; Graaf, Cees de; Janssen, Ignace M.C. ; Schijns, Wendy ; Aarts, Edo O. ; Jager, Gerry ; Boesveldt, Sanne - \ 2018
Behavioural Brain Research 353 (2018). - ISSN 0166-4328 - p. 194 - 202.
Endocannabinoid - Energy-density - fMRI - Food cues - Ghrelin - Obesity - Olfactory - Reward - Roux-en-Y gastric bypass surgery - Visual

Background: After Roux-en-Y gastric bypass (RYGB) surgery, patients report a shift in food preferences away from high-energy foods. Objective: We aimed to elucidate the potential mechanisms underlying this shift in food preferences by assessing changes in neural responses to food pictures and odors before and after RYGB. Additionally, we investigated whether altered neural responsivity was associated with changes in plasma endocannabinoid and ghrelin concentrations. Design: 19 RYGB patients (4 men; age 41 ± 10 years; BMI 41 ± 1 kg/m2 before; BMI 36 ± 1 kg/m2 after) participated in this study. Before and two months after RYGB surgery, they rated their food preferences using the Macronutrient and Taste Preference Ranking Task and BOLD fMRI responses towards pictures and odors of high-, and low-energy foods and non-food items were measured. Blood samples were taken to determine plasma endocannabinoid and ghrelin concentrations pre- and post-surgery. Results: Patients demonstrated a shift in food preferences away from high-fat/sweet and towards low-energy/savory food products, which correlated with decreased superior parietal lobule responsivity to high-energy food odor and a reduced difference in precuneus responsivity to high-energy versus low-energy food pictures. In the anteroventral prefrontal cortex (superior frontal gyrus) the difference in deactivation towards high-energy versus non-food odors reduced. The precuneus was less deactivated in response to all cues. Plasma concentrations of anandamide were higher after surgery, while plasma concentrations of other endocannabinoids and ghrelin did not change. Alterations in appetite-related hormone concentrations did not correlate with changes in neural responsivity. Conclusions: RYGB leads to changed responsivity of the frontoparietal control network that orchestrates top-down control to high-energy food compared to low-energy food and non-food cues, rather than in reward related brain regions, in a satiated state. Together with correlations with the shift in food preference from high- to low-energy foods this indicates a possible role in new food preference formation.

Thought for urban food planning : The Oosterwold (NL) experiment to normalise agriculture in urban planning
Jansma, J.E. ; Janssen-Jansen, L. ; Valk, A.J.J. van der; Wertheim-Heck, S.C.O. - \ 2018
In: Proceedings of Annual AESOP congress 10-14 July 2018, Gothenburg - p. 10 - 13.
Proceedings of Annual AESOP congress 10-14 Juli 2018, Gothenburg
AgroDataCube: A Big Open Data collection for Agri-Food Applications
Janssen, H. ; Janssen, S.J.C. ; Knapen, M.J.R. ; Meijninger, W.M.L. ; Randen, Y. van; Riviere, I.J. la; Roerink, G.J. - \ 2018
AgroDataCube provides a large collection of both open data and derived data for use in agri-food applications. Open data has been collected from, among others, the Dutch government, Rijkswaterstaat, KNMI and Wageningen University and Research. Interoperability and re-usability create added value to the data. The AgroDataCube aims at building on common agro-semantic standards and stimulates the use of open source data and to exchange open knowledge across the agri-food chain.
Altered neural inhibition responses to food cues after Roux-en-Y Gastric Bypass
Zoon, H.F.A. ; Bruijn, S.E.M. de; Jager, G. ; Smeets, P.A.M. ; Graaf, C. de; Janssen, I.M.C. ; Schijns, W. ; Deden, L. ; Boesveldt, S. - \ 2018
Biological Psychology 137 (2018). - ISSN 0301-0511 - p. 34 - 41.
Bariatric surgery - fMRI - Food preferences - go/no-go - Impulsivity - Inhibitory control - Weight-Loss

Background: Roux-en-Y gastric bypass (RYGB) surgery is a highly effective weight-loss intervention that often reduces preference and intake of high-energy foods. Research into the neural mechanisms behind this shift has mainly focused on reward processing of food cues. However, the ability to successfully control food intake and thereby weight-loss also depends on inhibitory control capacity. We investigated whether RYGB leads to alterations in neural inhibitory control in response to food cues. Methods: A food-specific go/no-go task with pictures of high-energy (desserts) and low-energy foods (vegetables), was used to assess neural inhibition responses before and after RYGB with functional magnetic resonance imaging. Data from 18 morbidly obese patients (15 females; age 41 ± 11 years; BMI 42 ± 4 kg/m2 before; BMI 36 ± 4 kg/m2 after) were analysed. Pre- and post-RYGB BOLD fMRI responses were compared for response inhibition towards high- and low-energy foods. Participants were tested in a satiated state. Results: Response inhibition to high-energy foods was associated with increased activation of the right lateral prefrontal cortex (PFC), right medial PFC, dorsolateral PFC, right middle cingulate cortex and the right inferior frontal operculum (involved in inhibitory control), after compared to before surgery. Response inhibition to low-energy foods elicited diminished post- compared to pre-surgery responses in the left superior temporal pole, right parahippocampal gyrus and right hypothalamus (involved in metabolic control). Conclusion: Neural changes indicate improved response inhibition towards high-energy food cues, altered influence of metabolic control during response inhibition towards low-energy food cues and a more positive attitude to both high-energy and low-energy food after RYGB. Alterations in neural circuits involved in inhibitory control, satiety signalling and reward processing may contribute to effective weight-loss after RYGB.

Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genes
Daas, Martinus J.A. ; Nijsse, Bart ; Weijer, Antonius H.P. van de; Groenendaal, Bart W.A.J. ; Janssen, Fons ; Oost, John van der; Kranenburg, Richard van - \ 2018
BMC Biotechnology 18 (2018)1. - ISSN 1472-6750
CBP - Cellulase - Geobacillus - Metagenome - β-Xylosidase

Background: Consolidated bioprocessing (CBP) is a cost-effective approach for the conversion of lignocellulosic biomass to biofuels and biochemicals. The enzymatic conversion of cellulose to glucose requires the synergistic action of three types of enzymes: exoglucanases, endoglucanases and β-glucosidases. The thermophilic, hemicellulolytic Geobacillus thermodenitrificans T12 was shown to harbor desired features for CBP, although it lacks the desired endo and exoglucanases required for the conversion of cellulose. Here, we report the expression of both endoglucanase and exoglucanase encoding genes by G. thermodenitrificans T12, in an initial attempt to express cellulolytic enzymes that complement the enzymatic machinery of this strain. Results: A metagenome screen was performed on 73 G. thermodenitrificans strains using HMM profiles of all known CAZy families that contain endo and/or exoglucanases. Two putative endoglucanases, GE39 and GE40, belonging to glucoside hydrolase family 5 (GH5) were isolated and expressed in both E. coli and G. thermodenitrificans T12. Structure modeling of GE39 revealed a folding similar to a GH5 exo-1,3-β-glucanase from S. cerevisiae. However, we determined GE39 to be a β-xylosidase having pronounced activity towards p-nitrophenyl-β-d-xylopyranoside. Structure modelling of GE40 revealed its protein architecture to be similar to a GH5 endoglucanase from B. halodurans, and its endoglucanase activity was confirmed by enzymatic activity against 2-hydroxyethylcellulose, carboxymethylcellulose and barley β-glucan. Additionally, we introduced expression constructs into T12 containing Geobacillus sp. 70PC53 endoglucanase gene celA and both endoglucanase genes (M1 and M2) from Geobacillus sp. WSUCF1. Finally, we introduced expression constructs into T12 containing the C. thermocellum exoglucanases celK and celS genes and the endoglucanase celC gene. Conclusions: We identified a novel G. thermodenitrificans β-xylosidase (GE39) and a novel endoglucanase (GE40) using a metagenome screen based on multiple HMM profiles. We successfully expressed both genes in E. coli and functionally expressed the GE40 endoglucanase in G. thermodenitrificans T12. Additionally, the heterologous production of active CelK, a C. thermocellum derived exoglucanase, and CelA, a Geobacillus derived endoglucanase, was demonstrated with strain T12. The native hemicellulolytic activity and the heterologous cellulolytic activity described in this research provide a good basis for the further development of G. thermodenitrificans T12 as a host for consolidated bioprocessing.

Klimaatverandering en kwelders: verdrinken of verjongen?
Vader, Lotte ; Kuiters, A.T. - \ 2018
In: Buigen of barsten / Schaminée, Joop, Janssen, John, Zeist : KNNV uitgeverij (Vegetatiekundige monografieën 7) - ISBN 9789050116602 - p. 96 - 123.
Revisiting the enzymatic kinetics of pepsin using isothermal titration calorimetry
Luo, Q. ; Chen, Dongxin ; Boom, R.M. ; Janssen, A.E.M. - \ 2018
Food Chemistry 268 (2018). - ISSN 0308-8146 - p. 94 - 100.
Pepsin is the first protease that food proteins encounter in the digestive tract. However, most of the previous studies on the enzymatic kinetics of pepsin were based on the hydrolysis of small synthetic peptides, due to the limitations in methodology and the complexity of protein substrate. To better understand the role of pepsin in protein digestion, we used isothermal titration calorimetry to study the enzymatic kinetics of pepsin with bovine serum albumin as the substrate. We found that pepsin has a higher catalytic rate at lower pH, while its affinity to substrate is lower. At the same pH, pepsin has lower activity and affinity at higher ionic strengths. We found contrasting kinetic parameters for pepsin-catalyzed hydrolysis of bovine serum albumin and of small synthetic peptides. Time-dependent kinetics also showed that pepsin has lower efficiency towards intermediate peptides during hydrolysis.
In vitro gastric digestion of protein-based structured food : An engineering approach
Luo, Qi - \ 2018
University. Promotor(en): Remko Boom, co-promotor(en): Anja Janssen. - Wageningen : Wageningen University - ISBN 9789463437707 - 174
Regulation of angiopoietin-like 4 and lipoprotein lipase in human adipose tissue
Dijk, Wieneke ; Schutte, Sophie ; Aarts, Edo O. ; Janssen, Ignace M.C. ; Afman, Lydia ; Kersten, Sander - \ 2018
Journal of Clinical Lipidology 12 (2018)3. - ISSN 1933-2874 - p. 773 - 783.
ANGPTL4 - Human adipose tissue - Lipid metabolism - LPL - Triglycerides
Background: Elevated plasma triglycerides are increasingly viewed as a causal risk factor for coronary artery disease. One protein that raises plasma triglyceride levels and that has emerged as a modulator of coronary artery disease risk is angiopoietin-like 4 (ANGPTL4). ANGPTL4 raises plasma triglyceride levels by inhibiting lipoprotein lipase (LPL), the enzyme that catalyzes the hydrolysis of circulating triglycerides on the capillary endothelium. Objective: The objective of the present study was to assess the association between ANGPTL4 and LPL in human adipose tissue, and to examine the influence of nutritional status on ANGPTL4 expression. Methods: We determined ANGPTL4 and LPL mRNA and protein levels in different adipose tissue depots in a large number of severely obese patients who underwent bariatric surgery. Furthermore, in 72 abdominally obese subjects, we measured ANGPTL4 and LPL mRNA levels in subcutaneous adipose tissue in the fasted and postprandial state. Results: ANGPTL4 mRNA levels were highest in subcutaneous adipose tissue, whereas LPL mRNA levels were highest in mesenteric adipose tissue. ANGPTL4 and LPL mRNA levels were strongly positively correlated in the omental and subcutaneous adipose tissue depots. In contrast, ANGPTL4 and LPL protein levels were negatively correlated in subcutaneous adipose tissue, suggesting a suppressive effect of ANGPTL4 on LPL protein abundance in subcutaneous adipose tissue. ANGPTL4 mRNA levels were 38% higher in the fasted compared to the postprandial state. Conclusion: Our data provide valuable insights into the relationship between ANGPTL4 and LPL in human adipose tissue, as well as the physiological function and regulation of ANGPTL4 in humans.
Lipid metabolism in Nannochloropsis gaditana during nitrogen starvation
Janssen, Jorijn H. - \ 2018
University. Promotor(en): Rene Wijffels, co-promotor(en): Maria Barbosa. - Wageningen : Wageningen University - ISBN 9789463437691 - 164
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