Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Records 1 - 20 / 21

  • help
  • print

    Print search results

  • export

    Export search results

  • alert
    We will mail you new results for this query: q=Koenraad
Check title to add to marked list
How to us Nile Red, a selective fluorescent stain for microalgal neutral lipids
Alemán-Nava, Gibrán S. ; Cuellar-Bermudez, Sara P. ; Cuaresma, María ; Bosma, Rouke ; Muylaert, Koenraad ; Ritmann, Bruce E. ; Parra, Roberto - \ 2016
Journal of Microbiological Methods 128 (2016). - ISSN 0167-7012 - p. 74 - 79.
Biodiesel - Microalgae - Neutral lipids - Nile Red - Protocol

The use of Nile Red for rapid monitoring of the neutral lipid content in microalgae has gained interest over the last decade, since neutral lipids are feedstock for renewable transportation fuel. In this review, we discuss the main considerations needed to make an NR protocol reliable for staining neutral lipids in microalgae. Cell wall permeability must be enhanced by using stain carriers: DMSO (5% v/v to 25% v/v), glycerol (0.1 to 0.125 mg/mL), or EDTA (3.0 to 3.8 mg/mL). Temperatures between 30 and 40 °C facilitate the diffusion of NR through the cell wall without incurring excess quenching. Good NR-lipid interaction requires using a low NR/cell ratio; the NR concentration must be between 0.25 μg/mL and 2.0 μg/mL, and the cell concentration > 5 × 104 cells/mL. In order to have the maximum and stable NR fluorescence, it is necessary to scan the excitation/emission wavelengths for up to a 40-min of incubation time. We outline a five-step method to customize the Nile Red protocol to a specific strain: 1) Evaluate the strain's suitability by checking for the presence of neutral lipid, 2) Select of the best excitation/emission wavelength, 3) Optimization of incubation time, stain carrier, dye concentration, and temperature, 4) Prepare single-strain algal cultures with different lipid contents to calibrate NR fluorescence with neutral-lipid content, and 5) Correlate NR fluorescence intensity to neutral lipid content for the same strain. Once the protocol is customized, the NR method allows for rapid and reliable monitoring of neutral lipid content of a microalgae strain.

Procesregeling bestuursrecht. Uniformering van bestuursrecht door rechterlijke beleidsregels
Meulen, B.M.J. van der; Koenraad, L.M. - \ 2001
Trema 2001 (2001). - p. 369 - 373.
Epidemiological aspects of thermophilic Campylobacter in water-related environments: A review.
Koenraad, P.M.F.J. ; Rombouts, F.M. ; Notermans, S.H.W. - \ 1997
Water Environment Research 69 (1997). - ISSN 1061-4303 - p. 52 - 63.
Subtyping of Campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular techniques.
Koenraad, P.M.F.J. ; Ayling, R. ; Hazeleger, W.C. ; Rombouts, F.M. ; Newell, D.G. - \ 1996
In: Campylobacter, Helicobacter, and related organisms - p. 197 - 201.
Two-dimensional protein profiles and fatty-acid compositions in coccoid forms of Campylobacter jejuni.
Hazeleger, W.C. ; Janse, J.D. ; Koenraad, P.M.F.J. ; Beumer, P.R. ; Rombouts, F.M. ; Abee, T. - \ 1996
In: Campylobacter, Helicobacter, and related organisms - p. 119 - 122.
Short-term evidence of Campylobacter in a treatment plant and drain water of a connected poultry abattoir.
Koenraad, P.M.F.J. ; Jacobs-Reitsma, W.F. ; Beumer, R.R. ; Rombouts, F.M. - \ 1996
Water Environment Research 68 (1996). - ISSN 1061-4303 - p. 188 - 193.
Prevalence of Campylobacter in Dutch sewage purification plants
Koenraad, P.M.F.J. - \ 1995
Agricultural University. Promotor(en): F.M. Rombouts; S.H.W. Notermans. - S.l. : S.n. - ISBN 9789054854128 - 149
afvalverwerking - rioolwater - afvalwater - samenstelling - chemische eigenschappen - fysische eigenschappen - campylobacter - microbiologie - afvalwaterbehandeling - zuiveringsinstallaties - waste treatment - sewage - waste water - composition - chemical properties - physical properties - microbiology - waste water treatment - purification plants
<p><em>Campylobacter</em> bacteria are an important cause of bacterial gastro-enteritis in man. Although food of animal origin is the main source of human infection, a casecontrol study in the United States of America showed that 8% of all campylobacteriosis cases could be attributed to consumption of contaminated surface water. In this thesis the prevalence of <em>Campylobacter</em> in sewage purification plants was investigated in order to obtain more information on the survival of this pathogen in aquatic environments. A survey carried out on three municipal plants showed that sewage and surface waters are frequently contaminated with <em>Campylobacter.</em> The contamination of sewage was higher when meat-processing industries were present in the drainage area of these plants. Indeed, drain water of a poultry abattoir contained high numbers of <em>Campylobacter</em> . It is clear that other sources contribute far lower numbers to sewage. Furthermore, the aquatic <em>Campylobacter</em> isolates were more resistant to quinolones and to ampicillin when a meat-processing industry was draining its waste on the sewerage.<p>The purification process reduced the numbers of <em>Campylobacter</em> , but this pathogen was not eliminated completely. The prevalence and reduction were not correlated with enviromnental parameters, such as water temperature and oxygen pressure.<p>The phenomenon of the transformation of spiral <em>Campylobacter</em> cells to coccoid cells was also investigated. Determination of several physiological parameters indicated that the contribution of the nonculturable, coccoid <em>Campylobacter</em> cells is to infection routes probably negligible.<p>Considering the observed occurrence of <em>Campylobacter</em> in surface waters, in the context of the reported dose-response model for this pathogen, it can be concluded that the role of surface waters in the epidemiology of <em>Campylobacter</em> may be underestimated.
Methods for the detection of Campylobacter in sewage: evaluation of efficacy of enrichment and isolation media, applicability of Polymerase Chain Reaction and Latex Agglutination Assay.
Koenraad, P.M.F.J. ; Giesendorf, B.A.J. ; Henkens, M.H.C. ; Beumer, R.R. ; Quint, W.G.V. - \ 1995
Journal of Microbiological Methods 23 (1995). - ISSN 0167-7012 - p. 309 - 320.
The speciation and subtyping of campylobacter isolates from sewage plants and waste water from a connected poultry abattoir using molecular techniques.
Koenraad, P.M.F.J. ; Ayling, R. ; Hazeleger, W.C. ; Rombouts, F.M. ; Newell, D.G. - \ 1995
Epidemiology and Infection 115 (1995)3. - ISSN 0950-2688 - p. 485 - 494.
Antibiotic susceptibility of campylobacter isolates from sewage and poultry abattoir drain water.
Koenraad, P.M.F.J. ; Jacobs-Reitsma, W.F. ; Laan, T. van der; Beumer, R.R. ; Rombouts, F.M. - \ 1995
Epidemiology and Infection 115 (1995)3. - ISSN 0950-2688 - p. 475 - 483.
Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of Campylobacter jejuni.
Hazeleger, W.C. ; Janse, H.D. ; Koenraad, P.M.F.J. ; Beumer, R.R. ; Rombouts, F.M. ; Abee, T. - \ 1995
Applied and Environmental Microbiology 61 (1995). - ISSN 0099-2240 - p. 2713 - 2719.
Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of C. jejuni.
Hazeleger, W.C. ; Janse, J.D. ; Koenraad, P.M.F.J. ; Beumer, R.R. ; Rombouts, F.M. ; Abee, T. - \ 1995
In: Abstract 8th Int. workshop on Campylobacters, helicobacters and related organisms., Winchester, UK - p. 71 - 71.
Non-culturable coccoid forms of Campylobacter jejuni.
Hazeleger, W.C. ; Koenraad, P.M.F.J. ; Beumer, R.R. ; Abee, T. ; Rombouts, F.M. - \ 1994
In: Report WHO consultation Epidemiology and control of Campylobacteriosis in humans and animals Bilthoven : - p. 111 - 114.
Survival of Campylobacter in sewage plants.
Koenraad, P.M.F.J. ; Hazeleger, W.C. ; Laan, T. van der; Beumer, R.R. ; Rombouts, F.M. - \ 1994
In: Report WHO consultation Epidemiology and control of Campylobacteriosis in humans and animals Bilthoven : - p. 115 - 119.
Survey of Campylobacter spp. in sewage plants in The Netherlands.
Koenraad, P.M.F.J. ; Hazeleger, W.C. ; Laan, T. van der; Beumer, R.R. ; Rombouts, F.M. - \ 1994
Food Microbiology 11 (1994). - ISSN 0740-0020 - p. 65 - 73.
Thermophilic campylobacters in the waste water of a poultry slaughterhouse and in the influx and the efflux of a sewage farm.
Koenraad, P.M.F.J. ; Jacobs-Reitsma, W.F. ; Hazeleger, W.C. ; Beumer, R.R. ; Rombouts, F.M. - \ 1993
In: Abstract 7th Int. Workshop Campylobacter, Helicobacter and related organisms, Brussels (1993)
Investigation of the spiral and non-culturable coccoid form of Campylobacter jejuni.
Hazeleger, W.C. ; Koenraad, P.M.F.J. ; Abee, T. ; Beumer, R.R. ; Rombouts, F.M. - \ 1993
In: Abstract 7th Int. Workshop Campylobacter, Helicobacter and related organisms, Brussels - p. 39 - 39.
Survival of Campylobacter jejuni/coli on a model surface.
Koenraad, P.M.F.J. ; Beumer, R.R. ; Hazeleger, W.C. ; Rombouts, F.M. - \ 1992
In: Abstract 3rd World Congr. Foodborne infections and intoxications Vol. 2, Berlin - p. 1160 - 1160.
Campylobacter jejuni in sewage treatment systems.
Koenraad, P.M.F.J. ; Hazeleger, W.C. ; Beumer, R.R. ; Rombouts, F.M. - \ 1992
In: Proc. 3rd World Congr. Foodborne infections and intoxications Vol. 1, Berlin - p. 144 - 146.
Arbeidstijden op varkensbedrijven
Roelofs, P. ; Habroken, K. ; Koenraad, H. - \ 1991
Praktijkonderzoek varkenshouderij 5 (1991)4. - ISSN 1382-0346 - p. 2 - 5.
varkens - arbeidskunde - werkuren - pigs - work study - working hours
Op 15 varkensbedrijven zijn arbeidstijden verzameld. De varkenshouders hebben gedurende drie weken bijgehouden hoeveel tijd ze hebben besteed aan de meest voorkomende werkzaamheden. Deze waren van tevoren onderscheiden en omschreven. Uit de resultatenkunnen taaktijden worden afgeleid.
Check title to add to marked list
<< previous | next >>

Show 20 50 100 records per page

 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.