Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Crystal structure of Brugia malayi venom allergen-like protein-1 (BmVAL-1), a vaccine candidate for lymphatic filariasis
Darwiche, Rabih ; Lugo, Fernanda ; Drurey, Claire ; Varossieau, Koen ; Smant, Geert ; Wilbers, Ruud H.P. ; Maizels, Rick M. ; Schneiter, Roger ; Asojo, Oluwatoyin A. - \ 2018
International Journal for Parasitology 48 (2018)5. - ISSN 0020-7519 - p. 371 - 378.
Brugia malayi is a causative agent of lymphatic filariasis, a major tropical disease. The infective L3 parasite stage releases immunomodulatory proteins including the venom allergen-like proteins (VALs), which are members of the SCP/TAPS (Sperm-coating protein/Tpx/antigen 5/pathogenesis related-1/Sc7) superfamily. BmVAL-1 is a major target of host immunity with >90% of infected B. malayi microfilaraemic cases being seropositive for antibodies to BmVAL-1. This study is part of ongoing efforts to characterize the structures and functions of important B. malayi proteins. Recombinant BmVAL-1 was produced using a plant expression system, crystallized and the structure was solved by molecular replacement and refined to 2.1 Å, revealing the characteristic alpha/beta/alpha sandwich topology of eukaryotic SCP/TAPS proteins. The protein has more than 45% loop regions and these flexible loops connect the helices and strands, which are longer than predicted based on other parasite SCP/TAPS protein structures. The large central cavity of BmVAL-1 is a prototypical CRISP cavity with two histidines required to bind divalent cations. The caveolin-binding motif (CBM) that mediates sterol binding in SCP/TAPS proteins is large and open in BmVAL-1 and is N-glycosylated. N-glycosylation of the CBM does not affect the ability of BmVAL-1 to bind sterol in vitro. BmVAL-1 complements the in vivo sterol export phenotype of yeast mutants lacking their endogenous SCP/TAPS proteins. The in vitro sterol-binding affinity of BmVAL-1 is comparable with Pry1, a yeast sterol transporting SCP/TAPS protein. Sterol binding of BmVAL-1 is dependent on divalent cations. BmVAL-1 also has a large open palmitate-binding cavity, which binds palmitate comparably to tablysin-15, a lipid-binding SCP/TAPS protein. The central cavity, CBM and palmitatebinding cavity of BmVAL-1 are interconnected within the monomer with channels that can serve as pathways for water molecules, cations and small molecules.
Países Baixos: pastoreo para a sociedade ou beneficio?
Pol-van Dasselaar, Agnes van den - \ 2017
Analysis of results of biomass forest inventory in northeastern amazon for development of REDD+ carbon project
Mello, L.N.C. ; Ribeiro Sales, Marcio ; Rosa, Luiz P. - \ 2016
Anais da Academia Brasileira de Ciencias 88 (2016)1. - ISSN 0001-3765 - p. 55 - 64.
Carbon forest - Climate change - Deforestation in Amazon - REDD+ projects

In Brazil, a significant reduction in deforestation rates occurred during the last decade. In spite of that fact, the average annual rates are still too high, approximately 400.000 ha/year (INPE/Prodes). The projects of emissions reduction through avoided deforestation (REED+) are an important tool to reduce deforestation rates in Brazil. Understanding the amazon forest structure, in terms of biomass stock is key to design avoided deforestation strategies. In this work, we analyze data results from aboveground biomass of 1,019.346,27 hectares in the state of Pará. It was collected data from 16,722 trees in 83 random independent plots. It was tested 4 allometric equations, for DBH > 10cm: Brown et al. (1989), Brown and Lugo (1999), Chambers et al. (2000), Higuchi et al. (1998). It revealed that the biggest carbon stock of above ground biomass is stocked on the interval at DBH between 30cm and 80cm. This biomass compartment stocks 75.70% of total biomass in Higuchi et al. (1998) equation, 75.56% of total biomass in Brown et al. (1989) equation, 78.83% of total biomass in Chambers et al. (2000) equation, and 73.22% in Brown and Lugo (1999) equation.

Learning by Practice, High-Pressure Student Ateliers
Timmermans, W. ; Cilliers, J. ; Garcia, A. ; Koolen, A. ; Woudstra, A. ; Dijk, T. van - \ 2014
In: The Design Charette: Ways to envision sustainable futures / Roggema, R., Dordrecht : Springer Science + Business Media - ISBN 9789400770317 - p. 279 - 293.
Two international and interdisciplinary high pressure student ateliers have been done and evaluated, one to explore possible future scenario’s for Strofylia (Greece) as requested by local and regional authorities and stakeholders, the other to put urban agriculture on the Lugo (Galicia, Spain) agenda. Aims of the ateliers have been to create a student learning environment in practice and make use of the expected conceptual flexibility and creativity of the students to come up with unexpected ideas. We have used interviews to evaluate the ateliers. The ateliers provide a rich learning by practice environment and offer new perspectives to practitioners and policy makers. Not much study has been carried out on high-pressure student ateliers in land use planning and landscape architecture. An outline is presented for a more thorough study of this type of ateliers to understand advantages and shortcomings of different atelier approaches.
Air scrubbing techniques for the reduction of gaseous emissions from livestock farms. Actual knowledge in the Netherlands and possibilities for implementation on Spanish farms.
Estellés, F. ; Melse, R.W. ; Ogink, N.W.M. ; Torres, A.G. - \ 2009
In The Netherlands, packed-bed biotrickling filters and acid scrubbers for removal of ammonia from exhaust air of animal houses are off-the-shelf techniques for ammonia removal. At the moment a new generation of so-called "multi-pollutant scrubbers" is being developed and tested that also removes odour and particulate matter from the air. Air cleaning systems are unusual in Spanish farms. The study of these techniques and their adaptation to Spanish farms, can be an interesting issue in order to achieve the national gas emission reduction commitments, and also to reduce social nuisances.
The way you do, it matters : a case study: farming economically in Galician dairy agroecosystems in the context of a cooperative
Dominguez Garcia, M.D. - \ 2007
University. Promotor(en): Jandouwe van der Ploeg, co-promotor(en): X.S. Fernández. - [S.l.] : S.n. - ISBN 9789085047964 - 194
rurale sociologie - plattelandsontwikkeling - melkveehouderij - sociale economie - sociale verandering - landgebruik - modernisering - stijl - milieueffect - agrarische bedrijfsvoering - coöperatieve landbouw - spanje - melkveehouderijsystemen - economische verandering - agro-ecosystemen - agro-ecologie - rural sociology - rural development - dairy farming - socioeconomics - social change - land use - modernization - style - environmental impact - farm management - cooperative farming - spain - dairy farming systems - economic change - agroecosystems - agroecology
This thesis aims to study the sustainability of agriculture in Galicia (Spain) in a new and integrative way. Therefore not only economic but also social and ecological data and interest are drawn into the analysis. Farming, undoubtedly, is an economic activity. However, in order to obtain and market the final product natural resources are used and transformed. When pollution and depletion or exhaustion of those resources takes place, the future of the activity is at stake. Furthermore, social relations play an important role. The use of labour, for instance, is based on economic deliberations but also regulated by social interests. Social and political factors are at stake at the institutional level as well. These factors determine not only the orientation of agricultural production but also its volume, and prices. Agriculture is a multi-dimensional activity. It is an ecological activity by its use of natural resources. It is a social activity as production and consumption are based on a specific organisation of social relations. And it is a cultural activity as agriculture roots in a way of living which is time- and place specific and characterized by locally and historically specific norms and values. In short, it is not only an activity that generates economic value. In order to study the sustainability of an agricultural system, it is therefore important to analyse its impact on different (social, economic and ecological) domains but also to look into their interrelation and, hence, interaction. Theoretically there are two possible ways of interaction: (1) the interaction between the different domains has a positive effect and strengthen the sustainability of the whole system (synergy), or (2) the different domains counteract each other and a positive effect in one domains works to the detriment of another domain (trade off effect). In this thesis an integrative approach was chosen which made use of the insights and methods of different disciplines: ecological economics, agro-ecology and sociology. On the basis of the integrative approach we analysed if and how the economic, ecological and social effects of an agricultural system vary depending on the chosen style of farming. In order to understand actual Galician agriculture it is necessary to know the past. The first part of the thesis describes the recent history of Galician agriculture. Since the Second World War the traditional peasant agro-ecosystem transformed into an industrial system; this period is also described as a process of de-structuring. It included the dismantling of the traditional agro-ecosystem in force until the middle of the 20th century; and the recessive dynamics derived from the most recent process of modernisation throughout the period 1960-2005. In this thesis, the traditional agroecosystem is defined as an articulated and joint management of cattle, crops and nature with a high degree of integration and interaction. It analyses of political and socio-demographic factors that have jeopardised the continuation of that model. The dismantling of the traditional agroecosystem is effective during the last process of modernisation after 1960. Its effects come to the fore quite clearly in the changes in land use during that period, demonstrated by the evolution of the Useful Agricultural Area and the Total Area, as well as by the reduction in the number of farms. The main features of this process of modernisation are the alignment of farm management strategies based on intensification, specialisation and the increasing dependency on external market inputs as regards both biological and, specially, mechanical technology. Income squeeze, reduction of labour and abandonment of productive natural resources are the main consequences of that process so far. Although the study of the farming dynamics at the regional level suggests homogeneity of management-strategies, the second part of the thesis -looking for heterogeneity- shows that the practice is actually heterogeneous. Heterogeneity in the practice or the different ways of doing are encountered by applying the Farming Styles approach and methodology. Styles of farming are analysed at the level of the farmer and his/her farm, within the context of a cooperative of services, Os Irmandinos, located in Ribadeo (Lugo, Galicia). In a following step we analysed the social, economic and ecological impact of every style by means of different indicators. In this way we wanted to find out if the way of doing matters in terms of sustainability. This is interesting not only in order to evaluate the farmers' response of the past, but also to see which of the styles might have promising results also for the future and the possibility to achieve a more sustainable agriculture and balanced rural development. Within the different styles of farming, the style characterized as 'Farming Economically' comes to the fore as an interesting point of departure for the construction of sustainability in a multi-dimensional way.

Transition of an abandoned Dutch agrosilvopastoral landscape to "new wilderness" by extensive grazing with free-ranging cattle and horses
Kuiters, A.T. - \ 2005
In: Silvopastoralism and sustainable land management. - Wallingford (UK) : CABI - ISBN 1845930010 - p. 148 - 149.
An assessment of the role of grazing in European habitats / Un análisis sobre el papel del pastoreo en los hábitats europeos
Bunce, R.G.H. ; Pérez-Soba, M. ; Elbersen, B.S. ; Wingerden, W.K.R.E. van - \ 2004
In: Silvopastoralism and sustainable management; book of abstracts / Silvopastoralismo y manejo sostenible; libro de actas. - Lugo (Spain) : S.n. - ISBN 8496351033 - p. 188 - 188.
Transition of an abandoned Dutch agro-silvopastoral landscape to "new wilderness" by extensive grazing with free-ranging cattle and horses / Transición de un paisaje holandés agro-silvopastoral abandonado a un espacio de pastoreo extensive y libre con bovino y equino
Kuiters, A.T. - \ 2004
In: Silvopastoralism and sustainable management; book of abstracts / Silvopastoralismo y manejo sostenible; libro de actas. - Lugo (Spain) : S.n. - ISBN 8496351033 - p. 84 - 84.
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