Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Zoonotic endocarditis in a man, The Netherlands
Sleutjens, Janneke ; Meijer, Dennie ; Meregalli, Paola G. ; Bakker, Leendert ; Wagenaar, Jaap A. ; Duim, Birgitta ; Zomer, Aldert - \ 2019
Emerging Infectious Diseases 25 (2019)1. - ISSN 1080-6040 - p. 180 - 182.

In 2017, endocarditis caused by Streptococcus equi subspecies zooepidemicus was diagnosed in a man in the Netherlands who had daily contact with horses. Whole-genome sequencing of isolates from the man and his horses confirmed the same clone, indicating horse-to-human transmission. Systematic reporting of all zoonotic cases would help with risk assessment.

Resistentie tegen trips en prei
Scholten, O.E. ; Burger-Meijer, K. ; Henken, G. ; Vosman, B. - \ 2018
Genotyping by sequencing to identify diagnostic regions in Fusarium oxysporum f. sp. cubense Tropical Race 4 and applications in disease epidemiology
Salacinas, Maricar ; Ordonez, N. ; Mendes, O. ; Schoen, C.D. ; Seidl, M.F. ; Meijer, H.J.G. ; Kema, G.H.J. - \ 2018
Global genotyping by sequencing.
Meijer, Harold - \ 2018
Bovine lactoferrin enhances TLR7-mediated responses in plasmacytoid dendritic cells in elderly women : Results from a nutritional intervention study with bovine lactoferrin, GOS and Vitamin D
Splunter, Marloes van; Perdijk, Olaf ; Fick-Brinkhof, Henriëtte ; Feitsma, Anouk L. ; Floris-Vollenbroek, Esther G. ; Meijer, Ben ; Brugman, Sylvia ; Savelkoul, Huub F.J. ; Hoffen, Els van; Neerven, R.J.J. van - \ 2018
Frontiers in Immunology 9 (2018). - ISSN 1664-3224
aging - bovine lactoferrin - GOS - inflammation - mDCs - pDCs - TLR stimulation - Vitamin D

During aging the immune system is dysregulated. Especially plasmacytoid dendritic cells (pDCs) and myeloid DCs (mDCs) have reduced Toll like receptor (TLR)-mediated responses resulting in increased susceptibility to infections. Consumption of bovine lactoferrin (bLF) has been shown to reduce infections with viruses. Galacto-oligosacharides (GOS) and Vitamin D are associated with reduced pro-inflammatory cytokine levels in serum, and increased TLR7/8 responses, respectively. A double-blind placebo-controlled nutritional intervention study in elderly women was performed, to investigate the potential of bLF, GOS, and Vitamin D to restore TLR responsiveness of pDCs and mDCs and to reduce inflammatory markers in serum. The nutritional intervention group (n = 15) received bLF for 3 weeks, followed by 3 weeks of bLF + GOS, and subsequently 3 weeks of bLF + GOS + Vitamin D. The placebo group (n = 15) received maltodextrin for 9 weeks. Every 3 weeks, blood was collected and TLR responses of pDCs and mDCs, and inflammation-related markers in serum were measured. After 3 weeks of bLF supplementation, increased TLR7/8 and TLR1/2 responses were observed in pDCs of the nutritional intervention group compared to the placebo group. When the effects of the entire nutritional intervention were investigated, increased TLR1/2 mediated responses in mDCs were observed, and in serum sVCAM tended to decrease. Finally, based on the RAND-36 questionnaire physical function tended to improve in the intervention group. Since especially TLR7-mediated responses in pDCs were enhanced after bLF supplementation compared to placebo, this suggests that bLF may contribute to antiviral responses mediated by pDC in elderly women.

Aged mice display altered numbers and phenotype of basophils, and bone marrow-derived basophil activation, with a limited role for aging-associated microbiota
Beek, Adriaan A. Van; Fransen, Floris ; Meijer, Ben ; Vos, Paul de; Knol, Edward F. ; Savelkoul, Huub F.J. - \ 2018
Immunity and Ageing 15 (2018)1.
Aging - Basophils - Bone marrow - Immunity - Microbiota - Spleen

Background: The influence of age on basophils is poorly understood, as well as the effect of aging-associated microbiota on basophils. Therefore, we studied the influence of aging and aging-associated microbiota on basophil frequency and phenotype, and differentiation from basophil precursors. Results: Basophils became more abundant in bone marrow (BM) and spleens of 19-month-old mice compared with 4-month-old mice. Aged basophils tended to express less CD200R3 and more CD123, both in BM and spleen. Differences in microbiota composition with aging were confirmed by 16S sequencing. Microbiota transfers from young and old mice to germ-free recipients revealed that CD11b tended to be lowered on splenic basophils by aging-associated microbiota. Furthermore, abundance of Alistipes, Oscillibacter, Bacteroidetes RC9 gut group, and S24-7 family positively correlated and CD123 expression, whereas Akkermansia abundance negatively correlated with basophils numbers. Subsequently, we purified FcϵRIα+CD11c-CD117- BM-derived basophils and found that those from aged mice expressed lower levels of CD11b upon stimulation. Higher frequencies of IL-4+ basophils were generated from basophil precursors of aged mice, which could be reproduced in basophils derived from germ-free recipients of aging-associated microbiota. Conclusions: Collectively, these results show the influence of aging on basophils. Furthermore, this study shows that aging-associated microbiota altered activation of BM-derived basophils in a similar fashion as observed in BM-derived basophils from aged mice.

QTL mapping of insect resistance components of Solanum galapagense
Vosman, Ben ; Kashaninia, Atiyeh ; van’t Westende, Wendy ; Meijer-Dekens, Fien ; Eekelen, Henriëtte van; Visser, Richard G.F. ; Vos, Ric C.H. de; Voorrips, Roeland E. - \ 2018
Theoretical and Applied Genetics (2018). - ISSN 0040-5752 - 11 p.

Key message: QTLs for insect resistance parameters, trichome type IV development, and more than 200 non-volatile metabolites, including 76 acyl sugars, all co-locate at the end of Chromosome 2 of Solanum galapagense. Abstract: Host plant resistance is gaining importance as more and more insecticides are being banned due to environmental concerns. In tomato, resistance towards insects is found in wild relatives and has been attributed to the presence of glandular trichomes and their specific phytochemical composition. In this paper, we describe the results from a large-scale QTL mapping of data from whitefly resistance tests, trichome phenotyping and a comprehensive metabolomics analysis in a recombinant inbred line population derived from a cross between the cultivated Solanum lycopersicum and the wild relative S. galapagense, which is resistant to a range of pest insects. One major QTL (Wf-1) was found to govern the resistance against two different whitefly species. This QTL co-localizes with QTLs for the presence of trichomes type IV and V, as well as all 76 acyl sugars detected and about 150 other non-volatile phytochemicals, including methyl esters of the flavonols myricetin and quercetin. Based on these results, we hypothesize that Wf-1 is regulating the formation of glandular trichome type IV on the leaf epidermis, enabling the production and accumulation of bioactive metabolites in this type of trichomes.

A SIX1 homolog in Fusarium oxysporum f.sp. Cubense tropical race 4 contributes to virulence towards Cavendish banana
Widinugraheni, S. ; Niño-Sánchez, J. ; Does, H.C. van der; Dam, P. van; García-Bastidas, F.A. ; Subandiyah, S. ; Meijer, H.J.G. ; Kistler, H.C. ; Kema, G.H.J. ; Rep, M. - \ 2018
PLoS One 13 (2018)10. - ISSN 1932-6203

The fungus Fusarium oxysporum f.sp. cubense (Focub) causes Fusarium wilt of banana. Focub strains are divided into races according to their host specificity, but which virulence factors underlie these interactions is currently unknown. In the F. oxysporum f.sp. lycopersici (Fol)-tomato system, small secreted fungal proteins, called Six proteins, were identified in the xylem sap of infected plants. The Fol Six1 protein contributes to virulence and has an avirulence function by activating the I-3 immune receptor of tomato. The Focub tropical race 4 (TR4) genome harbors three SIX1 homologs: SIX1a, b and c. In this study, the role of Focub-SIX1a in pathogenicity was evaluated since this homolog is present in not only TR4 but also in other races. A deletion mutant of the SIX1a gene from Focub TR4 strain II5 was generated (FocubΔSIX1a) and tested in planta. Mutants were found to be severely compromised in their virulence. Ectopic integration of the Focub-SIX1a gene in the FocubΔSIX1a strain restored virulence to wild type levels. We conclude that Focub-SIX1a is required for full virulence of Focub TR4 towards Cavendish banana.

Phytophthora infestans small phospholipase D-like proteins elicit plant cell death and promote virulence
Meijer, Harold J.G. ; Schoina, Charikleia ; Wang, Shutong ; Bouwmeester, Klaas ; Hua, Chenlei ; Govers, Francine - \ 2018
Molecular Plant Pathology (2018). - ISSN 1464-6722
calcium - late blight disease - oomycete - phospholipases - phospholipids - signal peptide

The successful invasion of host tissue by (hemi-)biotrophic plant pathogens is dependent on modifications of the host plasma membrane to facilitate the two-way transfer of proteins and other compounds. Haustorium formation and the establishment of extrahaustorial membranes are probably dependent on a variety of enzymes that modify membranes in a coordinated fashion. Phospholipases, enzymes that hydrolyse phospholipids, have been implicated as virulence factors in several pathogens. The oomycete Phytophthora infestans is a hemibiotrophic pathogen that causes potato late blight. It possesses different classes of phospholipase D (PLD) proteins, including small PLD-like proteins with and without signal peptide (sPLD-likes and PLD-likes, respectively). Here, we studied the role of sPLD-like-1, sPLD-like-12 and PLD-like-1 in the infection process. They are expressed in expanding lesions on potato leaves and during in vitro growth, with the highest transcript levels in germinating cysts. When expressed in planta in the presence of the silencing suppressor P19, all three elicited a local cell death response that was visible at the microscopic level as autofluorescence and strongly boosted in the presence of calcium. Moreover, inoculation of leaves expressing the small PLD-like genes resulted in increased lesion growth and greater numbers of sporangia, but this was abolished when mutated PLD-like genes were expressed with non-functional PLD catalytic motifs. These results show that the three small PLD-likes are catalytically active and suggest that their enzymatic activity is required for the promotion of virulence, possibly by executing membrane modifications to support the growth of P. infestans in the host.

A global genetic diversity analysis of Fusarium oxysporum f.sp. cubense : the Panama disease pathogen of banana
Ordóñez R., Nadia - \ 2018
Wageningen University. Promotor(en): Gert Kema, co-promotor(en): Michael Seidl; Harold Meijer. - Wageningen : Wageningen University - ISBN 9789463432986 - 156
Regionalisation in poultry developmen in Eastern Africa
Vernooij, Adriaan ; Masaki, Mackenzie N. ; Meijer-Willems, Daphne - \ 2018
Wageningen : Wageningen Livestock Research (Wageningen Livestock Research rapport 1121) - 47
This regional study of East Africa’s (EA) poultry sector was commissioned by the Food and Business Knowledge platform in 2017 with deference to the growing interest of Dutch companies in East Africa. The poultry sector in EA has been growing rapidly for the past 5 years driven by: rapid urbanization; the growth of the middle class in EA; rise in number of quick services restaurants in urban areas in EA; and growing need for animal protein. Of the four EA economies, Kenya’s poultry sector is the most mature. However, all the other three economies have in the past five years made significant strides towards developing and growing their own poultry sector.In order to justify the case for regional approach to poultry development in EA 3 key issues were addressed. First the availability and production of good quality feed at competitive prices. Governments in the region have made conscious policy changes to incentivize the import of raw materials and ingredients for feed into the region. In addition to this certain countries in EA such as Tanzania and Rwanda have made poultry specific strategies to catalyse growth of their poultry sectors. Whereas collective initiatives are in principal good for sectoral development at a regional level, history shows that there are the significant challenges to overcome. For instance, clarity on cross border trade in maize, oil seeds and oil seed cake is necessary for a sound regional approach to feed. The disparity in terms of available land for maize cultivation, the cost price and eventual market price for maize differs significantly per country in EA. Each county in EA has a competitive advantage that could complement the others. However, finding these complementarities and capitalising them for the greater good of the region will give rise to significant benefits for each EA member state. Secondly, the availability of DOCs across EA is a challenge. Kenya trades with Uganda and Uganda trades with Rwanda when it comes to DOCs. There is a shortage of DOCs in EA and there is a seasonality both in availability and price that makes it difficult for farmers to plan ahead and manage their costs.Lastly, access to markets, the East African Community (EAC) is both an economic and political block. Citizens of the member states are in principal allowed to work, do business and trade with one another freely. Though the EAC is the most advanced regional economic block in Africa it still faces certain challenges to implementing certain policies collectively. For instance in February of 2018 all EA member states agreed to remove VAT on all raw materials imported for feed manufacturing. Of the four member states, three have already tabled this policy in parliament and one has not. Free access to a market of approximately 140 million people will drive investments in the sector across the entire value chain. Such initiatives should be given priority and expedited in order to catalyse investments and growth in the EA poultry sector.Complimentary to the three issues above is knowledge and training at vocational and tertiary levels in EA. There is need for better more specific training and education in the poultry sector. A case in point is in EA veterinary doctors are in principal experts for all livestock and poultry is one amongst many of the courses they would take during their 4 to 6 years study. Upon completion most are not equipped to immediately enter into the poultry sector. Most farms and companies in the sector have been forced to develop on the job training programs that allow high performers to learn poultry specific knowledge. In addition to knowledge and training collective investment in regulation and enforcement of good biosecurity and animal health practices would go a long way to reduce and or manage disease outbreaks in the region. In the context of this study, it is observed that in EA a majority of poultry farmers are either small or medium scale farmers. Collective investments in feed, DOCs, animal health, knowledge transfer, capacity building, training and access to markets will greatly assist them become better farmers. In recognition of the increased significance of the poultry sector, various financing and financial institutions have become interested in poultry farmers and other poultry sector value chain actors.6 | Wageningen Livestock Research Report 1121These developments coupled with increasing demand for animal protein make the sector very attractive not only for local actors but also for the Dutch private sector.
Food Safety Issues Related to Uses of Insects for Feeds and Foods
Fels-Klerx, H.J. van der; Camenzuli, L. ; Belluco, S. ; Meijer, N. ; Ricci, A. - \ 2018
Comprehensive reviews in food science and food safety 17 (2018)5. - ISSN 1541-4337 - p. 1172 - 1183.
edible insects - feed - food - review - safety

Edible insects are expected to become an important nutrient source for animals and humans in the Western world in the near future. However, before insects can be put on the market, the safety of their use for feed and food is warranted. This literature study was prepared to provide an overview of the actual knowledge of possible food safety hazards, including chemical, microbiological, and allergenic agents and prions, to human and animal health upon the use of insects for food and feed, and to highlight data gaps and suggest the way forward. From the data available, heavy metals of concern are cadmium in black soldier fly and arsenic in yellow mealworm larvae. Investigated mycotoxins do not seem to accumulate. Residues of pesticides, veterinary drugs, and hormones, as well as dioxins and PCBs, are sometimes found in insects. Contamination of insects with pathogens to human health is a consequence of a combination of the substrates used and the farming and processing steps applied. Insects harbor a wide variety of microorganisms, and some human pathogenic bacteria may be present. In addition, insects may harbor and transmit parasites. There is no evidence so far insects may harbor pathogenic viruses or prions, but they may act as vectors. Insects and insect-derived products may have allergenic potential. In this review, evidence on some safety aspects is displayed, and data gaps are identified. Recommendations are given for future research to fill the most relevant data gaps.

Droogte belangrijk bij onderzoek maisrassen
Groten, Jos ; Weijers, Gerrit - \ 2018
Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice
Shao, Jingxia ; Haider, Imran ; Xiong, Lizhong ; Zhu, Xiaoyi ; Hussain, Rana Muhammad Fraz ; Övernäs, Elin ; Meijer, Annemarie H. ; Zhang, Gaisheng ; Wang, Mei ; Bouwmeester, Harro J. ; Ouwerkerk, Pieter B.F. - \ 2018
PLoS One 13 (2018)7. - ISSN 1932-6203

The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.

Oviposition preference of three lepidopteran species is not affected by previous aphid infestation in wild cabbage
Li, Yehua ; Meijer, Davy ; Dicke, Marcel ; Gols, Rieta - \ 2018
Entomologia Experimentalis et Applicata 166 (2018)5. - ISSN 0013-8703 - p. 402 - 411.
Brassica oleracea - Brevicoryne brassicae - host plant selection - insect herbivores - JA-SA crosstalk - Mamestra brassicae - Pieris brassicae - plant induction - Plutella xylostella - preference–performance hypothesis

Several studies have shown that pre-infestation with aphids can improve plant quality for herbivorous caterpillars. This effect is often explained by the negative crosstalk between specific plant defence, signal-transduction pathways induced by aphids and caterpillars, respectively. However, in these studies caterpillars are introduced on the plants by the researcher, whereas in nature, the adult mother often chooses the food plants for her offspring. According to the preference–performance hypothesis adult females should choose oviposition sites that result in optimal performance and survival of their offspring. In this study, we investigated whether three lepidopteran species–Pieris brassicae (L.) (Pieridae), Plutella xylostella L. (Plutellidae) and Mamestra brassicae L. (Noctuidae)–prefer aphid-infested over clean plants. Adult females of the three species was given the choice between wild cabbage (Brassica oleracea L., Brassicaceae) plants infested with aphids, Brevicoryne brassicae (L.) (Hemiptera: Aphididae) for 3, 7, or 14 days vs. non-infested clean plants. Pieris brassicae females was also given the choice between plants dually infested with B. brassicae aphids and P. xylostella caterpillars when the order of infestation was varied. For oviposition, adult females of all three species did not discriminate between aphid-infested and clean plants, irrespective of the duration of aphid infestation. Also, P. brassicae females did not discriminate between sets of dually infested plants, irrespective of the order of infestation. Several mechanisms are discussed that could explain this lack of preference.

QTL mapping in diploid potato by using selfed progenies of the cross S. tuberosum × S. chacoense
Meijer, D. ; Viquez-Zamora, M. ; Eck, H.J. van; Hutten, R.C.B. ; Su, Y. ; Rothengatter, R. ; Visser, R.G.F. ; Lindhout, W.H. ; Heusden, A.W. van - \ 2018
Euphytica 214 (2018)7. - ISSN 0014-2336
Diploid potato - Homozygosity - Inbreeding - Self-compatibility

Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level.

Insights in the epidemiology and diversity of Fusarium oxysporum f. sp. cubense, the causal agent of Panama disease in banana
Kema, G.H.J. ; Garcia Bastidas, F.A. ; Ordonez Roman, N.I. ; Salacinas, Maricar ; Seidl, M.F. ; Thomma, B.P.H.J. ; Meijer, H.J.G. - \ 2018
Gewasbescherming 48 (2018)4/5/6. - ISSN 0166-6495 - p. 123 - 123.
Targeted and random genetic modification of the black Sigatoka pathogen Pseudocercospora fijiensis by Agrobacterium tumefaciens-mediated transformation
Díaz-Trujillo, Caucasella ; Kobayashi, Adilson K. ; Souza, Manoel ; Chong, Pablo ; Meijer, Harold J.G. ; Arango Isaza, Rafael E. ; Kema, Gert H.J. - \ 2018
Journal of Microbiological Methods 148 (2018). - ISSN 0167-7012 - p. 127 - 137.
New geographical insights of the latest expansion of fusarium oxysporum f.Sp. Cubense tropical race 4 into the greater mekong subregion
Zheng, Si Jun ; García-Bastidas, Fernando A. ; Li, Xundong ; Zeng, Li ; Bai, Tingting ; Xu, Shengtao ; Yin, Kesuo ; Li, Hongxiang ; Fu, Gang ; Yu, Yanchun ; Yang, Liu ; Nguyen, Huy Chung ; Douangboupha, Bounneuang ; Khaing, Aye Aye ; Drenth, Andre ; Seidl, Michael F. ; Meijer, Harold J.G. ; Kema, Gert H.J. - \ 2018
Frontiers in Plant Science 9 (2018). - ISSN 1664-462X
China - Fusarium wilt - Laos - Myanmar - Phytogeography - Single nucleotide polymorphism (SNP) - The Greater Mekong Subregion (GMS) - Vietnam
Banana is the most popular and most exported fruit and also a major food crop for millions of people around the world. Despite its importance and the presence of serious disease threats, research into this crop is limited. One of those is Panama disease or Fusarium wilt. In the previous century Fusarium wilt wiped out the “Gros Michel” based banana industry in Central America. The epidemic was eventually quenched by planting “Cavendish” bananas. However, 50 years ago the disease recurred, but now on “Cavendish” bananas. Since then the disease has spread across South-East Asia, to the Middle-East and the Indian subcontinent and leaped into Africa. Here, we report the presence of Fusariumoxysporumf.sp. cubense Tropical Race 4 (Foc TR4) in “Cavendish” plantations in Laos, Myanmar, and Vietnam. A combination of classical morphology, DNA sequencing, and phenotyping assays revealed a very close relationship between the Foc TR4 strains in the entire Greater Mekong Subregion (GMS), which is increasingly prone to intensive banana production. Analyses of single-nucleotide polymorphisms enabled us to initiate a phylogeography of Foc TR4 across three geographical areas—GMS, Indian subcontinent, and the Middle East revealing three distinct Foc TR4 sub-lineages. Collectively, our data place these new incursions in a broader agroecological context and underscore the need for awareness campaigns and the implementation of validated quarantine measures to prevent further international dissemination of Foc TR4.
Efficiency of organic stream conversion by black soldier fly larvae: a review of the scientific literature
Bosch, G. ; Veenenbos, M.E. ; Zanten, H.H.E. van; Meijer, N.P. ; Fels-Klerx, H.J. van der; Loon, J.J.A. van - \ 2018
Journal of Insects as Food and Feed 4 (2018)supplement 1. - ISSN 2352-4588 - p. S44 - S44.
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