Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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The badass and the asshole: violence and the positioned subjectivities of street youth in Mexico City
Gigengack, R.A. - \ 2014
In: Claiming the city: civil society mobilisation by the urban poor / Moksnes, H., Melin, M., Uppsala : Uppsala Centre for Sustainable Development - ISBN 9789198039153 - p. 172 - 184.
Effects of washing procedure, particle size and dilution on the distribution between non-washable, insoluble washable and soluble washable fractions in concentrate ingredients
Azarfar, A. ; Tamminga, S. ; Boer, H. - \ 2007
Journal of the Science of Food and Agriculture 87 (2007)13. - ISSN 0022-5142 - p. 2390 - 2398.
dairy-cows - protein - rumen - degradability - starch - energy - sacco - digestibility - degradation - feedstuffs
The effects of washing procedure, particle size and dilution on the distribution of non-washable (NWF), insoluble washable (ISWF) and soluble washable (SWF) fractions were studied. The effects of three washing procedures (Yang (Y), Melin (M) and in situ (IS)) on the size of NWF, ISWF and SWF in six concentrate ingredients (maize, barley, milo, peas, lupins and faba beans), ground at two different particle sizes, were compared. Method M was further developed (method SM) by reducing the dilution ratio; its effect on NWF, ISWF and SWF was compared. A new washing method was developed (method AA) which involved continuous washing of nylon bags in a centrifuge beaker; its effect on NWF, ISWF and SWF at different dilutions with water was compared with the IS, M, SM and Y methods. The effects of different dilutions on SWF and soluble true protein (STP) in six concentrate ingredients were studied. The effects of grain, washing method and particle size on the size of NWF and ISWF were significant, with significant interactions between grain and particle size, grain and washing method, particle size and washing method, but no interaction between grain type, washing method and particle size. In method Y the size of NWF was smaller than in the other methods. The results showed that, except in lupins, NWF in grains was significantly higher than in legume seeds. Increasing the particle size significantly increased NWF, whereas ISWF was decreased. The size of SWF in legume seeds was higher than in the grains. Increasing the dilution, increased STP in legume seeds, but not in grains.
Chemical characteristics and degradation of crude protein in the S-fraction of feed stuffs
Melin, M. ; Gierus, M. ; Vuuren, A.M. van; Meijer, G.A.L. - \ 2001
Lelystad : ID-Lelystad
Degradation of soluble crude protein in the rumen
Melin, M. ; Gierus, M. ; Vuuren, A.M. van; Meijer, G.A.L. - \ 2001
Journal of Dairy Science 84 (2001)suppl. 1. - ISSN 0022-0302 - p. 161 - 162.
Although CP in the soluble fraction lost during washing of nylon bags is often assumed to be fully and rapidly degraded in the rumen, its actual fate in the rumen is questioned. We developed a filtration procedure that mimics machine washing of nylon bags and enables a further characterization of this soluble fraction. The filtrate of 1-3 g of DM of ten feeds was collected in 100 ml of rinsing water. Feeds were two grass silages (GS1, 25% of DM, and GS2, 53% of DM), wet brewers grain silage (WBGS) and corn gluten feed silage (CGFS), 3 types of corn gluten feed (CGF1 to 3), soybean meal (SBM), lupine meal (LPM) and rape seed meal (RSM). DM and N losses of the filtration procedure and machine washing were compared in quadruplet samples. DM losses during filtration and from nylon bags ranged from 16% in WBGS to 46%in GS1 and were not different between procedures. N recovered in the filtrate ranged from 11.5% for SBM to 56.1% for GS1. The filtrate was centrifuged at 1500 g for 20 min. The pellet was analyzed for N in non soluble particles (NS). Soluble true proteins (TP) in the supernatant were precipitated with TCA (10% in the final volume), and the N in the remaining supernatant was defined as non-protein N (NPN). The NS fraction comprised 0% (GS1 and 2) to 87% (WBGS) of N in the filtrate. Significant amounts of TP were found in SBM (53%), LPM (30%) and RSM (27%). NPN ranged from 13% (WBGS) to 100% (GS1 and 2) of N in the filtrate. The degradation of the protein N (NS +TP) in the filtrate of 6 feeds was measured in rumen fluid using a modified Broderick method. After 4h of incubation the percentages of degraded protein were: 0% (WBGS and CGFS), 24% (CGF 2), 55% (RSM), 79% (LPM)and 80% (SBM). These results show that CP in the soluble fraction, as determined in the nylon bag technique, consists of different protein and non-protein fractions, that are not always rapidly and fully degraded in the rumen. The potential underestimation of the protein value of these feeds, calculated as ((NS + TP)/ N feed) x %undegraded4h,is2- 7 % for the concentrates, and 13 % and 26 % for CGFS and WBGS, respectively
Hormonema schizolunatum, a new species of dothideaceous black yeast from phyllosphere.
Middelhoven, W.J. ; Hoog, G.S. de - \ 1997
Antonie van Leeuwenhoek: : Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie 71 (1997)4. - ISSN 0003-6072 - p. 297 - 305.
Two black yeast isolates from plants from the Canary Islands (Spain) are described and illustrated. Absence of Woronin bodies at simple septal pores, local coralloid terminal hyphal cells, indeterminate thallus maturation, the presence of budding cells and local conversion to meristematic growth all indicate a relationship to the Dothideaceae (Dothideales, Ascomycota). Morphological properties were consistent with the genus Hormonema Lagerberg & Melin, as defined by presence of percurrent conidiogenous loci alongside undifferentiated hyphae, and results of PCR-ribotyping supported this classification. The isolates were judged to belong to a hitherto undescribed species, characterized in particular by curved conidia soon developing transverse septa. The physiological profile of this species is also described.
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