Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Foreword
Boonekamp, Piet M. ; Pieterse, Corné M.J. ; Govers, Francine ; Cornelissen, Ben J.C. - \ 2019
European Journal of Plant Pathology 154 (2019)1. - ISSN 0929-1873 - p. 1 - 3.
Groen als integrerend kader : naar een vitale binnenstad van Doetinchem
Cate, Bram ten; Grashof-Bokdam, Carla ; Kruit, Jeroen ; Schoonderbeek, Jeroen ; Weggemans, Ruben ; Stuijt, Rob ; Duin, Duco ; Akker, Stefan van den; Brouwers, Chanine ; Dell’Oro, Matteo ; Lührmann, Jullian ; Neer, Sibylla ; Pieterse, Chantal - \ 2019
Wageningen : Wageningen University & Research, Wetenschapswinkel (Wageningen University & Research Wetenschapswinkel rapport 347) - ISBN 9789463435482 - 30
Johanna Westerdijk (1881–1961) – the impact of the grand lady of phytopathology in the Netherlands from 1917 to 2017
Boonekamp, Piet M. ; Pieterse, Corné M.J. ; Govers, Francine ; Cornelissen, Ben J.C. - \ 2019
European Journal of Plant Pathology 154 (2019)1. - ISSN 0929-1873 - p. 11 - 16.
One century ago, on February 10, 1917, Johanna Westerdijk delivered her inaugural speech at Utrecht University entitled “New directions in phytopathological research”. By doing so, she became the very first female professor in the Netherlands and set the stage for many female professors who followed her example. Besides her remarkable performance as a role model for women in science, Johanna Westerdijk was also a pioneer in the global science field of phytopathology, which rapidly emerged at the end of the 19th century and reached maturity during the course of her scientific career.
Comparative review on microbiota manipulation: lessons from fish, plants, livestock and human research
Brugman, S. ; Ikeda-Ohtsubo, W. ; Braber, S. ; Folkerts, G. ; Pieterse, C.M.J. ; Bakker, P.A.H.M. - \ 2018
Frontiers in Nutrition 5 (2018). - ISSN 2296-861X - 15 p.
During recent years the impact of microbial communities on the health of their host (being plants, fish, and terrestrial animals including humans) has received increasing attention. The microbiota provides the host with nutrients, induces host immune development and metabolism, and protects the host against invading pathogens (1–6). Through millions of years of co-evolution bacteria and hosts have developed intimate relationships. Microbial colonization shapes the host immune system that in turn can shape the microbial composition (7–9). However, with the large scale use of antibiotics in agriculture and human medicine over the last decades an increase of diseases associated with so-called dysbiosis has emerged. Dysbiosis refers to either a disturbed microbial composition (outgrowth of possible pathogenic species) or a disturbed interaction between bacteria and the host (10). Instead of using more antibiotics to treat dysbiosis there is a need to develop alternative strategies to combat disturbed microbial control. To this end, we can learn from nature itself. For example, the plant root (or “rhizosphere”) microbiome of sugar beet contains several bacterial species that suppress the fungal root pathogen Rhizoctonia solani, an economically important fungal pathogen of this crop (11). Likewise, commensal bacteria present on healthy human skin produce antimicrobial molecules that selectively kill skin pathogen Staphylococcus aureus. Interestingly, patients with atopic dermatitis (inflammation of the skin) lacked antimicrobial peptide secreting commensal skin bacteria (12). In this review, we will give an overview of microbial manipulation in fish, plants, and terrestrial animals including humans to uncover conserved mechanisms and learn how we might restore microbial balance increasing the resilience of the host species.
How can we define "optimal microbiota?": a comparative review of structure and functions of microbiota of animals, fish and plants in agriculture
Ikeda-Ohtsubo, W. ; Brugman, S. ; Warden, C.H. ; Rebel, Annemarie ; Folkerts, G. ; Pieterse, C.M.J. - \ 2018
Frontiers in Nutrition 5 (2018)113. - ISSN 2296-861X - 18 p.
All multicellular organisms benefit from their own microbiota, which play important roles in maintaining the host nutritional health and immunity. Recently, the number of studies on the microbiota of animals, fish, and plants of economic importance is rapidly expanding and there are increasing expectations that productivity and sustainability in agricultural management can be improved by microbiota manipulation. However, optimizing microbiota is still a challenging task because of the lack of knowledge on the dominant microorganisms or significant variations between microbiota, reflecting sampling biases, different agricultural management as well as breeding backgrounds. To offer a more generalized view on microbiota in agriculture, which can be used for defining criteria of “optimal microbiota” as the goal of manipulation, we summarize here current knowledge on microbiota on animals, fish, and plants with emphasis on bacterial community structure and metabolic functions, and how microbiota can be affected by domestication, conventional agricultural practices, and use of antimicrobial agents. Finally, we discuss future tasks for defining “optimal microbiota,” which can improve host growth, nutrition, and immunity and reduce the use of antimicrobial agents in agriculture.
Combining QTL mapping with transcriptome and metabolome profiling reveals a possible role for ABA signaling in resistance against the cabbage whitefly in cabbage
Broekgaarden, Colette ; Pelgrom, Koen T.B. ; Bucher, Johan ; Dam, Nicole M. van; Grosser, Katharine ; Pieterse, Corné M.J. ; Kaauwen, Martijn van; Steenhuis, Greet ; Voorrips, Roeland E. ; Vos, Martin de; Vosman, Ben ; Worrich, Anja ; Wees, Saskia C.M. van - \ 2018
PLoS ONE 13 (2018)11. - ISSN 1932-6203

Whiteflies are among the world's most significant agricultural pests and chemical insecticides are extensively used to reduce crop damage to acceptable levels. However, nearly all insecticides pose a threat to the environment and alternative control methods, such as breeding of crop varieties that are inherently insect-resistant, are needed. Previously, a strong source of plant-age dependent resistance to the cabbage whitefly (Aleyrodes proletella) has been identified in the modern white cabbage (Brassica oleracea var. capitata) variety Rivera. However, nothing is known about the molecular mechanisms or the genes involved in this resistance. In the present study, a multidisciplinary approach combining transcriptome and metabolome profiling with genetic mapping was used to identify the molecular players of whitefly resistance in cabbage. Transcriptome profiles of young (susceptible) and older (resistant) Rivera plants were analyzed using RNA sequencing. While many genes involved in general processes were differentially expressed between both ages, several defense-related processes were overrepresented in the transcriptome profile of older plants. Hormone measurements revealed that jasmonic acid (JA) levels decreased upon whitefly infestation at both plant ages. Interestingly, abscisic acid (ABA) levels showed contrasting effects in response to whitefly infestation: ABA levels were reduced in young plants but induced in older plants upon whitefly feeding. Auxin levels were significantly lower in older plants compared with young plants, independent of whitefly presence, while glucosinolate levels were higher. Additionally, whitefly performance was monitored in an F2 population derived from a cross between Rivera and the susceptible white cabbage variety Christmas Drumhead. Significant QTL intervals were mapped on chromosome 2 and 9 for oviposition rate and whitefly adult survival, respectively. Several genes that were higher expressed in older plants and located in the identified QTL intervals were orthologous to Arabidopsis genes that have been related to ABA signaling, suggesting a role for ABA in the regulation of resistance towards whiteflies. Our results show that combining different omics approaches is a useful strategy to identify candidate genes underlying insect resistance.

Genome-wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
Proietti, Silvia ; Caarls, Lotte ; Coolen, Silvia ; Pelt, Johan A. van; Wees, Saskia C.M. van; Pieterse, Corné M.J. - \ 2018
Plant, Cell & Environment 41 (2018)10. - ISSN 0140-7791 - p. 2342 - 2356.
Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA‐regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome‐wide association (GWA) study on natural genetic variation in Arabidopsis thaliana for the effect of SA and ABA on the JA pathway. We treated 349 Arabidopsis accessions with methyl JA (MeJA), or a combination of MeJA and either SA or ABA, after which expression of the JA‐responsive marker gene PLANT DEFENSIN1.2 (PDF1.2) was quantified as a readout for GWA analysis. Both hormones antagonized MeJA‐induced PDF1.2 in the majority of the accessions but with a large variation in magnitude. GWA mapping of the SA‐ and ABA‐affected PDF1.2 expression data revealed loci associated with crosstalk. GLYI4 (encoding a glyoxalase) and ARR11 (encoding an Arabidopsis response regulator involved in cytokinin signalling) were confirmed by T‐DNA insertion mutant analysis to affect SA–JA crosstalk and resistance against the necrotroph Botrytis cinerea. In addition, At1g16310 (encoding a cation efflux family protein) was confirmed to affect ABA–JA crosstalk and susceptibility to Mamestra brassicae herbivory. Collectively, this GWA study identified novel players in JA hormone crosstalk with potential roles in the regulation of pathogen or insect resistance.
Thrips advisor : Exploiting thrips-induced defences to combat pests on crops
Steenbergen, Merel ; Abd-El-Haliem, Ahmed ; Bleeker, Petra ; Dicke, Marcel ; Escobar-Bravo, Rocio ; Cheng, Gang ; Haring, Michel A. ; Kant, Merijn R. ; Kappers, Iris ; Klinkhamer, Peter G.L. ; Leiss, Kirsten A. ; Legarrea, Saioa ; Macel, Mirka ; Mouden, Sanae ; Pieterse, Corné M.J. ; Sarde, Sandeep J. ; Schuurink, Robert C. ; Vos, Martin De; Wees, Saskia C.M. Van; Broekgaarden, Colette - \ 2018
Journal of Experimental Botany 69 (2018)8. - ISSN 0022-0957 - p. 1837 - 1848.
Cell-content feeder - effectors - herbivorous insect - phytohormone signalling - plant defence - specialized metabolites - thrips - virus - volatiles

Plants have developed diverse defence mechanisms to ward off herbivorous pests. However, agriculture still faces estimated crop yield losses ranging from 25% to 40% annually. These losses arise not only because of direct feeding damage, but also because many pests serve as vectors of plant viruses. Herbivorous thrips (Thysanoptera) are important pests of vegetable and ornamental crops worldwide, and encompass virtually all general problems of pests: they are highly polyphagous, hard to control because of their complex lifestyle, and they are vectors of destructive viruses. Currently, control management of thrips mainly relies on the use of chemical pesticides. However, thrips rapidly develop resistance to these pesticides. With the rising demand for more sustainable, safer, and healthier food production systems, we urgently need to pinpoint the gaps in knowledge of plant defences against thrips to enable the future development of novel control methods. In this review, we summarize the current, rather scarce, knowledge of thrips-induced plant responses and the role of phytohormonal signalling and chemical defences in these responses. We describe concrete opportunities for breeding resistance against pests such as thrips as a prototype approach for next-generation resistance breeding.

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways
Sousa, Diana Z. ; Visser, Michael ; Gelder, Antonie H. Van; Boeren, Sjef ; Pieterse, Mervin M. ; Pinkse, Martijn W.H. ; Verhaert, Peter D.E.M. ; Vogt, Carsten ; Franke, Steffi ; Kümmel, Steffen ; Stams, Alfons J.M. - \ 2018
Nature Communications 9 (2018)1. - ISSN 2041-1723
Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.
Towards a framework to access, compare and develop monitoring and evaluation of climate change adaptation in Europe
Klostermann, J.E.M. ; Sandt, K. van de; Harley, M. ; Hilden, M. ; Leiter, T. ; Minnen, J. van; Pieterse, N. ; Bree, L. van - \ 2018
Mitigation and Adaptation Strategies for Global Change 23 (2018)2. - ISSN 1381-2386 - p. 187 - 209.
Adaptation is increasingly recognised as essential when dealing with the adverse impacts of climate change on societies, economies and the environment. However, there is insufficient information about the effectiveness of adaption policies, measures and actions. For this reason, the establishment of monitoring programmes is considered to be necessary. Such programmes can contribute to knowledge, learning and data to support adaptation governance. In the European Union (EU), member states are encouraged to develop National Adaptation Strategies (NASs). The NASs developed so far vary widely because of differing views, approaches and policies. A number of member states have progressed to monitoring and evaluating the implementation of their NAS. It is possible to identify key elements in these monitoring programmes that can inform the wider policy learning process. In this paper, four generic building blocks for creating a monitoring and evaluation programme are proposed: (1) definition of the system of interest, (2) selection of a set of indicators, (3) identification of the organisations responsible for monitoring and (4) definition of monitoring and evaluation procedures. The monitoring programmes for NAS in three member states—Finland, the UK and Germany—were analysed to show how these elements have been used in practice, taking into account their specific contexts. It is asserted that the provision of a common framework incorporating these elements will help other member states and organisations within them in setting up and improving their adaptation monitoring programmes.
Soortenrijkdom Noordzee
Bos, O.G. ; Gittenberger, A. ; Boois, I.J. de; Asch, M. van; Wal, J.T. van der; Cremer, J.S.M. ; Hoorn, B. van der; Pieterse, S. ; Bakker, P.A.J. - \ 2017
- 1 p.
Genetic architecture of plant stress resistance: multi-trait genome-wide association mapping
Thoen, H.P.M. ; Davila Olivas, N.H. ; Kloth, K.J. ; Coolen, Silvia ; Huang, P. ; Aarts, M.G.M. ; Molenaar, J.A. ; Bakker, J. ; Bouwmeester, H.J. ; Broekgaarden, C. ; Bucher, J. ; Busscher-Lange, J. ; Cheng, X. ; Dijk-Fradin, E.F. van; Jongsma, M.A. ; Julkowska, Magdalena M. ; Keurentjes, J.J.B. ; Ligterink, W. ; Pieterse, Corné M.J. ; Ruyter-Spira, C.P. ; Smant, G. ; Schaik, C.C. van; Wees, Saskia C.M. van; Visser, R.G.F. ; Voorrips, R.E. ; Vosman, B. ; Vreugdenhil, D. ; Warmerdam, S. ; Wiegers, G.L. ; Heerwaarden, J. van; Kruijer, W.T. ; Eeuwijk, F.A. van; Dicke, M. - \ 2017
New Phytologist 213 (2017)3. - ISSN 0028-646X - p. 1346 - 1362.
Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only. Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker-trait associations in genome-wide association (GWA) analyses using tailored multi-trait mixed models. Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses. Plants appear to deploy broad-spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below-ground vs above-ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.
Soortenlijst Nederlandse Noordzee
Bos, O.G. ; Gittenberger, A. ; Boois, I.J. de; Asch, M. van; Wal, J.T. van der; Cremer, J. ; Hoorn, B. van der; Pieterse, S. ; Bakker, P.A.J. - \ 2016
Wageningen : Wageningen Marine Research (Wageningen Marine Research rapport C125/16) - 108
waterorganismen - soorten - soortenrijkdom - biodiversiteit - mariene ecologie - noordzee - nederland - aquatic organisms - species - species richness - biodiversity - marine ecology - north sea - netherlands
In dit rapport is een soortenlijst opgesteld voor de Nederlandse Noordzee in opdracht van het ministerie van Economische Zaken. Er is behoefte aan een concrete lijst van inheemse en niet-inheemse Noordzeesoorten omdat het rijk direct en indirect werkt aan behoud en duurzaam gebruik van de van nature voorkomende biodiversiteit van het Nederlandse deel van de Noordzee, aan beleid over “Bouwen met Noordzeenatuur” en aan het volgen van niet-inheemse soorten (exoten) in de Noordzee.
Exploring the genetics underlying the responses to consecutive combinations of biotic stresses and drought in Arabidopsis thaliana
Huang, Pingping - \ 2016
Wageningen University. Promotor(en): Maarten Koornneef, co-promotor(en): Mark Aarts. - Wageningen : Wageningen University - ISBN 9789462578593 - 291
arabidopsis thaliana - genetic models - stress - stress response - drought - botrytis - pieris (lepidoptera) - genetics - gene expression - genetische modellen - stressreactie - droogte - genetica - genexpressie

Plants growing in natural environments are exposed to a broad range of biotic (pathogen attack, insect herbivory, etc.) and abiotic factors (drought, extreme temperatures, UV radiation, salinity, etc.) that are known to cause stress symptoms in many species (Pareek et al., 2010; Robert-Seilaniantz et al., 2010). Biotic and abiotic stress-inducing determinants often adversely impact plant growth and development, frequently leading to severe annual yield losses in agricultural production (Pierik et al., 2013; Pieterse et al., 2012; Stam et al., 2014). In the research endeavors described in this thesis, Arabidopsis thaliana was used as a model organism to study plant responses to different sequential combinations of biotic factors (infection with Botrytis or herbivory by Pieris) and drought. The main objective was to identify genes that contribute to tolerance to the aforementioned sequential stress combinations. Genome-wide association (GWA) mapping and RNA sequencing (RNA-seq) approaches were used to identify combinatorial stress responsive genes. A number of candidate genes to combinatorial stress responses were identified by GWA analysis and RNA-seq. The physiological function of some candidate genes in different stress conditions were characterized using T-DNA insertion mutants and gene expression study. However, the physiological function of many allelic variants in stress conditions remain to be discovered. The study highlights the importance of an array of genes, crucial to the underlying defense processes, as targets for breeding by allele mining, ultimately aimed at improvement of crop tolerance to frequent combinations of stress factors.

Transcriptome dynamics of Arabidopsis during sequential biotic and abiotic stresses
Coolen, Silvia ; Proietti, Silvia ; Hickman, Richard ; Davila Olivas, Nelson H. ; Huang, Pingping ; Verk, Marcel C. van; Pelt, Johan A. van; Wittenberg, Alexander H.J. ; Vos, Martin de; Prins, Marcel ; Loon, Joop J.A. van; Aarts, Mark G.M. ; Dicke, Marcel ; Pieterse, Corné M.J. ; Wees, Saskia C.M. van - \ 2016
The Plant Journal 86 (2016)3. - ISSN 0960-7412 - p. 249 - 267.
Arabidopsis thaliana - Botrytis cinerea - combinatorial plant stress - drought stress - gene regulatory network - Pieris rapae - plant hormones - RNA-Seq - transcript profiling - 016-3950

In nature, plants have to cope with a wide range of stress conditions that often occur simultaneously or in sequence. To investigate how plants cope with multi-stress conditions, we analyzed the dynamics of whole-transcriptome profiles of Arabidopsis thaliana exposed to six sequential double stresses inflicted by combinations of: (i) infection by the necrotrophic fungus Botrytis cinerea, (ii) herbivory by chewing larvae of Pieris rapae, and (iii) drought stress. Each of these stresses induced specific expression profiles over time, in which one-third of all differentially expressed genes was shared by at least two single stresses. Of these, 394 genes were differentially expressed during all three stress conditions, albeit often in opposite directions. When two stresses were applied in sequence, plants displayed transcriptome profiles that were very similar to the second stress, irrespective of the nature of the first stress. Nevertheless, significant first-stress signatures could be identified in the sequential stress profiles. Bioinformatic analysis of the dynamics of co-expressed gene clusters highlighted specific clusters and biological processes of which the timing of activation or repression was altered by a prior stress. The first-stress signatures in second stress transcriptional profiles were remarkably often related to responses to phytohormones, strengthening the notion that hormones are global modulators of interactions between different types of stress. Because prior stresses can affect the level of tolerance against a subsequent stress (e.g. prior herbivory strongly affected resistance to B. cinerea), the first-stress signatures can provide important leads for the identification of molecular players that are decisive in the interactions between stress response pathways.

Effect of prior drought and pathogen stress on Arabidopsis transcriptome changes to caterpillar herbivory
Davila Olivas, Nelson H. ; Coolen, Silvia ; Huang, Pingping ; Severing, Edouard ; Verk, Marcel C. van; Hickman, Richard ; Wittenberg, Alexander H.J. ; Vos, Martin de; Prins, Marcel ; Loon, Joop J.A. van; Aarts, Mark G.M. ; Wees, Saskia C.M. van; Pieterse, Corné M.J. ; Dicke, Marcel - \ 2016
New Phytologist 210 (2016)4. - ISSN 0028-646X - p. 1344 - 1356.
Abiotic stress - Botrytis cinerea - Combined stresses - Insect herbivory - Multiple stresses - Pieris rapae - RNAseq - Transcriptome - 016-3939

In nature, plants are exposed to biotic and abiotic stresses that often occur simultaneously. Therefore, plant responses to combinations of stresses are most representative of how plants respond to stresses. We used RNAseq to assess temporal changes in the transcriptome of Arabidopsis thaliana to herbivory by Pieris rapae caterpillars, either alone or in combination with prior exposure to drought or infection with the necrotrophic fungus Botrytis cinerea. Pre-exposure to drought stress or Botrytis infection resulted in a significantly different timing of the caterpillar-induced transcriptional changes. Additionally, the combination of drought and P. rapae induced an extensive downregulation of A. thaliana genes involved in defence against pathogens. Despite a more substantial growth reduction observed for plants exposed to drought plus P. rapae feeding compared with P. rapae feeding alone, this did not affect weight increase of this specialist caterpillar. Plants respond to combined stresses with phenotypic and transcriptional changes that differ from the single stress situation. The effect of a previous exposure to drought or B. cinerea infection on transcriptional changes to caterpillars is largely overridden by the stress imposed by caterpillars, indicating that plants shift their response to the most recent stress applied.

Het verleden van onze toekomst : kroniek van de Koninklijke Nederlandse Plantenziektekundige Vereniging
Horsten, Jacques ; Bakker, J. ; Helder, J. ; Boonekamp, P.M. ; Cornelissen, Ben ; Dicke, M. ; Lenteren, J.C. van; Oers, M.M. van; Pieterse, G. ; Rabbinge, R. ; Thomma, B.P.H.J. ; Zadoks, J.C. - \ 2016
Wageningen : KNPV - 431 p.
Unravelling the one-carbon metabolism of the acetogen Sporomusa strain An4 by genome and proteome analysis
Visser, M. ; Pieterse, M.M. ; Pinkse, M.W.H. ; Nijsse, B. ; Verhaert, P.D.E.M. ; Vos, W.M. de; Schaap, P.J. ; Stams, A.J.M. - \ 2016
Environmental Microbiology 18 (2016)9. - ISSN 1462-2912 - p. 2843 - 2855.
The Sporomusa genus comprises anaerobic spore-forming acetogenic bacteria that stain Gram-negative. Sporomusa species typically grow with one-carbon substrates and N-methylated compounds. In the degradation of these compounds methyltransferases are involved. In addition, Sporomusa species can grow autotrophically with H2 and CO2 , and use a variety of sugars for acetogenic growth. Here we describe a genome analysis of Sporomusa strain An4 and a proteome analysis of cells grown under five different conditions. Comparison of the genomes of Sporomusa strain An4 and Sporomusa ovata strain H1 indicated that An4 is a S. ovata strain. Proteome analysis showed a high abundance of several methyltransferases, predominantly trimethylamine methyltransferases, during growth with betaine, while trimethylamine is one of the main end products of betaine degradation. In methanol degradation methyltransferases are also involved. In methanol utilizing methanogens two methyltransferases catalyze methanol conversion, methyltransferase 1 composed of subunits MtaB and MtaC and methyltransferase 2, also called MtaA. The two methyltransferase 1 subunits MtaB and MtaC were highly abundant when strain An4 was grown with methanol. However, instead of MtaA a methyltetrahydrofolate methyltransferase was synthesized. We propose a novel methanol degradation pathway in Sporomusa strain An4 that uses a methyltetrahydrofolate methyltransferase instead of MtaA
Genome sequencing of one-carbon degrading acetogenic bacteria Sporomusa An4
Visser, M. ; Pieterse, M.M. ; Pinkse, M.W.H. ; Nijsse, B. ; Verhaert, P.D.E.M. ; Vos, W.M. de; Stams, A.J.M. - \ 2015
PRJEB8443 - ERP009521 - Sporomusa
The genus Sporomusa contains, up to now, only one sequenced genome, that of S. ovata. Sequencing more strains is essential in understanding their common and specific physiology. Moreover, Sporomusa species typically grow with one-carbon substrates, but there are differences in their ability to grow with for example carbon monoxide. By comparing the already sequenced genomes of the type strain S. ovata with close related strains we can assess which genes are responsible for the physiological differences.
Goed Bezig: Resultaten van de Sociale Activering Strategie in de Regio Noord-Veluwe
Honigh de Vlaming, R. ; Haveman-Nies, A. ; Pieterse, C.M.J. ; Ploeg, B. van der; Renes, R.J. - \ 2014
Tijdschrift voor Gezondheidswetenschappen 92 (2014)8. - ISSN 1388-7491 - p. 325 - 333.
De gemeenten in de Noord-Veluwe werken aan preventie van overgewicht en overmatig alcoholgebruik bij kinderen binnen het programma Goed Bezig. Onderdeel hiervan is de Sociale Activering Strategie welke als doel heeft burgers en ondernemers te stimuleren en activeren tot een gemeenschappelijke verantwoordelijkheid voor gezonde leefgewoonten van jongeren van 4-16 jaar. Met een proces- en effectevaluatie is het resultaat van de Sociale Activering Strategie op het aantal gerealiseerde initiatieven en het aantal deelnemers, de participatiebereidheid van ouders en het leefstijlgedrag van jongeren onderzocht. Voor de effectevaluatie is gebruik gemaakt van een quasi-experimentele studieopzet met een interventie- en controlegroep en een voor- en nameting. De studiepopulatie betrof ouders van kinderen in de leeftijd van 4-16 jaar (n=192). Voor de procesevaluatie zijn logboeken gebruikt. De Sociale Activering Strategie is erin geslaagd om met name jonge kinderen te laten deelnemen aan leefstijlactiviteiten, welke zeer positief werden gewaardeerd. Ondanks het feit dat het aantal volwassenen dat is bereikt en een actieve bijdrage heeft geleverd aan de activiteiten beperkt was, zijn succesvolle bewonersinitiatieven gerealiseerd. De positieve verandering in het beweeggedrag van jongeren van wie de ouders actief betrokken zijn geweest, wijst erop dat een groeiende participatiebereidheid van ouders kan leiden tot een positieve gedragsverandering bij jongeren na een aantal jaren.
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