- D.W.B. Ducro-Steverink (1)
- V.J. Feron (4)
- W. Hazeleger (1)
- M.C.M. Jong de (1)
- A. Kidson (2)
- E.W. Kuijk (1)
- P. Langendijk (1)
- L.A.M.G. Leengoed van (1)
- C.J.A. Moorsel van (2)
- A. Nes van (1)
- T.A. Niewold (1)
- J.T. Oirschot van (1)
- B.A.J. Roelen (1)
- R. Roggeband (3)
- A.A.J.J.L. Rutten (4)
- E.J. Schoevers(older publications) (6)
- T.A.C. Schoevers (3)
- E. Schoevers (1)
- E.J. Schoevers (1)
- T. Tharasanit (1)
- J.H.M. Verheijden (2)
- J. Verheijden (1)
- A.P.M. Wolterbeek (4)
- C. Zijlstra (1)
Blastocyst morphology, actin cytoskeleton quality and chromosome content are correlated with embryo quality in the pig
Zijlstra, C. ; Kidson, A. ; Schoevers, E.J. ; Daemen, A.J.J.M. ; Tharasanit, T. ; Kuijk, E.W. ; Hazeleger, W. ; Ducro-Steverink, D.W.B. ; Colenbrander, B. ; Roelen, B.A.J. - \ 2008
Theriogenology 70 (2008)60. - ISSN 0093-691X - p. 923 - 935.
matured in-vitro - bovine embryos - oocyte maturation - sex-ratio - fertilization - vivo - aneuploidy - survival - system - cells
Embryo survival rates obtained after transfer of in vitro produced porcine blastocysts are very poor. This is probably related to poor quality of the embryos. The aim of the present study was to determine markers for good quality blastocysts. Therefore, we tried to link blastocyst morphology to several morphological and cell biological properties, and evaluated the survival of in vitro produced, morphologically classified, blastocysts following non-surgical transfer. In vitro and in vivo produced blastocysts were allocated to two groups (classes A and B) on the basis of morphological characteristics. The quality of their actin cytoskeleton, their total cell number, their ability to re-expand after cytochalasin-B treatment and the occurrence of numerical chromosome aberrations were studied and compared. In vivo produced blastocysts were used as a control. Our results indicate that the ability of blastocysts to re-expand after cytochalasin-B-induced actin depolymerization was positively correlated with the morphology of the blastocyst, and associated with the quality of the actin cytoskeleton. Chromosome analysis revealed that mosaicism is inherent to the in vitro production of porcine embryos, but also that in vivo produced blastocysts contained some non-diploid cells. In non-surgical embryo transfer experiments more recipients receiving class A blastocysts were pregnant on Day 20 than those receiving class B blastocysts. One recipient gave birth to six piglets from class A in vitro produced blastocysts, providing a verification of the enhanced viability of blastocysts that were scored as `good¿ on the basis of their morphology.
The effect of oviductal epithelial cell co-culture during in vitro maturation on sow oocyte morphology, fertilization and embryo development
Kidson, A. ; Schoevers, E. ; Langendijk, P. ; Verheijden, J. ; Colenbrander, B. ; Bevers, M. - \ 2003
Theriogenology 59 (2003)9. - ISSN 0093-691X - p. 1889 - 1903.
matured porcine oocytes - in-vitro - pig oocytes - perivitelline space - zona-pellucida - vivo - competence - polyspermy - sperm - glutathione
In vitro embryo production in the sow is challenged by poor cytoplasmic maturation, low sperm penetration and low normal fertilization, leading to the development of poor quality blastocysts containing a small number of nuclei. In prepubertal gilt oocytes, the presence of porcine oviductal epithelial cells (pOECs) during maturation increases cytoplasmic maturation and blastocyst development. These aspects, as well as blastocyst quality, may be improved when adult sow oocytes are matured with pOEC. Therefore, the effect of the presence of pOEC on sow oocyte morphology, fertilization and the progression of embryo development was evaluated. The pOEC were cultured in M199 for 18 h, then cultured in NCSU23 for 4 h before the oocytes were added. Oocytes from 2 to 6 mm follicles were matured in 500 ¿l NCSU23, with eCG and hCG, for 24 h, and then cultured with or without pOEC, in NCSU23 without hormones, for 18 h. In vitro fertilization took place in modified Tris-buffered medium, for 6 h, and the presumptive zygotes were then cultured for 162 h in NCSU23. Morphology of the IVM oocytes was compared to that of immature oocytes and in vivo matured MII oocytes from slaughtered sows in estrus. The in vitro matured oocytes had a greater diameter and a wider perivitelline space than the immature and in vivo matured MII oocytes (P
Pseudorabies virus is transmitted among vaccinated conventional pigs, but not among vaccinated SPF pigs
Nes, A. van; Jong, M.C.M. de; Schoevers, E.J. ; Oirschot, J.T. van; Verheijden, J.H.M. - \ 2001
Veterinary Microbiology 80 (2001). - ISSN 0378-1135 - p. 303 - 312.
Whereas the reproduction ratio (R) of pseudorabies virus (PRV) in vaccinated specific pathogen free (SPF) pigs without maternally derived antibodies under experimental conditions has repeatedly been shown to be significantly below 1, R in vaccinated conventional pigs in the field with maternally derived antibodies was significantly above 1. To exclude the difference in husbandry conditions as a cause for this discrepancy, we quantified and compared the transmission of PRV in both groups under identical experimental conditions. Whereas none of the SPF sentinel pigs became infected (R=0, significantly<1), all conventional sentinel pigs did become infected (R=2.5, significantly>1). Moreover, only one SPF pigs shed virus in saliva, the mean cumulative titre being almost a 100-fold less than in conventional pigs (17 pigs, P=0.003). In addition, the mean proliferation of peripheral blood lymphocytes in response to PRV antigens was significantly higher in SPF pigs than in conventional pigs at all points studied (P<0.0001). Moreover, the virus-neutralising antibody titre after vaccination was significantly higher in SPF pigs than in conventional pigs. We conclude that the discrepancy in transmission between vaccinated SPF pigs and vaccinated conventional pigs cannot be attributed to the experimental conditions.
|Effects of enrofloxacin on porcine phagocytic function
Schoevers, E.J. ; Leengoed, L.A.M.G. van; Verheijden, J.H.M. ; Niewold, T.A. - \ 1999
Antimicrobial Agents and Chemotherapy 43 (1999). - ISSN 0066-4804 - p. 2138 - 2143.
Influence of growth factors and medium composition on benzo[a]pyrene- and vitamin A-induced cell proliferation and differentiation in hamster tracheal epithelium in organ culture.
Wolterbeek, A.P.M. ; Ciotti, M.A.L.T. ; Schoevers, E.J. ; Roggeband, R. ; Baan, R.A. ; Feron, V.J. ; Rutten, A.A.J.J.L. - \ 1996
Toxicology in Vitro 10 (1996). - ISSN 0887-2333 - p. 359 - 369.
|A critical appraisal of intratracheal instillation of benzo[_]pyrene to Syrian golden hamers as a model in respiratory tract carcinogenesis.
Wolterbeek, A.P.M. ; Schoevers, E.J. ; Rutten, A.A.J.J.L. ; Feron, V.J. - \ 1995
Cancer Letters 89 (1995). - ISSN 0304-3835 - p. 107 - 116.
|Effect of vitamin A on benzo[a]pyrene induced DNA-adduct formation and cell proliferation in hamster tracheal epithelium.
Wolterbeek, A.P.M. ; Roggeband, R. ; Schoevers, E.J. ; Moorsel, C.J.A. van; Baan, R.A. ; Feron, V.J. ; Rutten, A.A.J.J.L. - \ 1994
Human and Experimental Toxicology 13 (1994). - ISSN 0960-3271 - p. 733 - 733.
Effect of vitamin A on B(a)P-DNA adduct formation and cell proliferation.
Wolterbeek, A.P.M. ; Roggeband, R. ; Schoevers, E.J. ; Moorsel, C.J.A. van; Ciotti, M.A.L.T. ; Baan, R.A. ; Feron, V.J. ; Rutten, A.A.J.J.L. - \ 1994
Toxicology Letters 74 (1994)suppl.1. - ISSN 0378-4274 - p. 95 - 95.
Proeven met eenige chemicaliën ter bestrijding van het wortelaaltje (Heterodera radicicola Greef)
Schoevers, T.A.C. - \ 1919
Wageningen : Veenman (Mededeelingen van de Landbouwhoogeschool en van de daaraan verbonden instituten dl. 15, [verh. 5])
Vreemde lichaampjes in zieke spinaziewortels
Schoevers, T.A.C. - \ 1919
Wageningen : Veenman (Mededeelingen van de Landbouwhoogeschool en van de daaraan verbonden instituten dl. 15, [verh. 4])
Proeven met eenige chemicaliën ter bestrijding van het wortelaaltje (Heterodera radicicola Greef) : voorloopige mededeeling
Schoevers, T.A.C. - \ 1917
Wageningen : Veenman (Mededeelingen van de Rijks Hoogere Land-, Tuin- en Boschbouwschool en van de daaraan verbonden instituten dl. 12, [verh. 2])