Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Records 1 - 20 / 243

  • help
  • print

    Print search results

  • export

    Export search results

  • alert
    We will mail you new results for this query: q=Schothorst
Check title to add to marked list
Prolonged intake of hyperproteic casein-based diet promotes a molecular environment leading to liver triacylglycerol deposition and increases markers of hepatic damage in rats
Schothorst, E.M. van; Keijer, J. ; Diaz, R. ; Palou, A. ; Oliver, P. - \ 2018
Introduction: High protein (HP) diets have been associated to body weight loss and positive metabolic effects on obese subjects. However, controversy exists on the effects of long-term intake of these diets, as more recent reports point to health risk and higher mortality. Liver is a key organ involved in macronutrient handling, thus, we aimed to analyse the effects of HP diets on liver metabolism and health.
Methods: We performed a transcriptome analysis on liver of healthy adult male Wistar rats fed for 4 months with a casein-rich HP diet and analysed adiposity and molecular parameters related to metabolic syndrome and liver injury.
Results: Compared to rats on a control diet, HP-fed animals, that ingested 2.3 times higher amount of protein than controls, showed a lower cumulative food intake and lower body weight; although this lower body weight was not related to decreased adiposity. HP-fed animals presented lower serum cholesterol levels and were apparently healthy according to parameters related to metabolic syndrome: no differences were found in circulating non-esterified fatty acids or triaclyglicerols (TG) in comparison to controls. In liver, long-term intake of the casein-rich diet had an impact on metabolic pathways related with amino acid uptake/metabolism and lipid synthesis, indicative of higher TG deposition. Liver transcriptomic analysis also revealed up-regulation of immune-related genes and changes in expression of genes involved in acid-base maintenance and oxidative stress, pointing to alterations in the pH balance due to the high acid load of the diet, which has been linked to liver/health damage. In line with these transcriptomic changes, clear functional signs of unhealthy effects, such increased liver TG content and increased serum markers of hepatic injury/inflammation (aspartate transaminase, C-reactive protein and TNF-alpha) were observed. Moreover, chronic intake of the HP diet produced a dramatic increase of hepatic HSP90, a marker of liver injury.
Conclusion: A drastic and prolonged increase in diet protein intake, resulting in a high acid load, induces a hepatic transcriptome signature reflecting increased TG deposition and increased levels of markers of liver/health injury.
Non-invasive continuous real-time in vivo analysis of microbial hydrogen production shows adaptation to fermentable carbohydrates in mice
Fernández-Calleja, José M.S. ; Konstanti, Prokopis ; Swarts, Hans J.M. ; Bouwman, Lianne M.S. ; Garcia-Campayo, Vicenta ; Billecke, Nils ; Oosting, Annemarie ; Smidt, Hauke ; Keijer, Jaap ; Schothorst, Evert M. van - \ 2018
Scientific Reports 8 (2018)1. - ISSN 2045-2322 - 16 p.

Real time in vivo methods are needed to better understand the interplay between diet and the gastrointestinal microbiota. Therefore, a rodent indirect calorimetry system was equipped with hydrogen (H2) and methane (CH4) sensors. H2 production was readily detected in C57BL/6J mice and followed a circadian rhythm. H2 production was increased within 12 hours after first exposure to a lowly-digestible starch diet (LDD) compared to a highly-digestible starch diet (HDD). Marked differences were observed in the faecal microbiota of animals fed the LDD and HDD diets. H2 was identified as a key variable explaining the variation in microbial communities, with specific taxa (including Bacteroides and Parasutterella) correlating with H2 production upon LDD-feeding. CH4 production was undetectable which was in line with absence of CH4 producers in the gut. We conclude that real-time in vivo monitoring of gases provides a non-invasive time-resolved system to explore the interplay between nutrition and gut microbes in a mouse model, and demonstrates potential for translation to other animal models and human studies.

Identification of early transcriptome-based biomarkers related to lipid metabolism in peripheral blood mononuclear cells of rats nutritionally programmed for improved metabolic health
Konieczna, J. ; Sánchez, J. ; Schothorst, E.M. van; Torrens, J.M. ; Bunschoten, J.E. ; Palou, M. ; Pico, C. ; Keijer, J. ; Palou, A. - \ 2018
GSE119955 - Rattus norvegicus - PRJNA490821
Moderate maternal calorie restriction during lactation protects rat offspring against obesity development in adulthood, due to an improved ability to handle and store excess dietary fuel. We used this model to identify early transcriptome-based biomarkers of metabolic health using peripheral blood mononuclear cells (PBMCs), an easily accessible surrogate tissue, by focusing on molecular markers of lipid handling. Male and female offspring of control and 20 % calorierestricted lactating dams (CR) were studied. At weaning, a set of pups was killed, and PBMCs were isolated for whole-genome microarray analysis. The remaining pups were killed at 6 months of age. CR gave lower body weight, food intake and fat accumulation, and improved levels of insulin and leptin throughout life, particularly in females. Microarray analysis of weaned rat PBMCs identified 278 genes significantly differentially expressed between control and CR. Among lipid metabolism-related genes, expression of Cpt1a, Lipe and Star was increased and Fasn, Lrp1 and Rxrb decreased in CR versus control, with changes fully confirmed by qPCR. Among them, Cpt1a, Fasn and Star emerged as particularly interesting. Transcript levels of Cpt1a in PBMCs correlated with their levels in WAT and liver at both ages examined; Fasn expression levels in PBMCs at an early age correlated with their expression levels in WAT; and early changes in Star expression levels in PBMCs correlated with their expression levels in liver and were sustained in adulthood. These findings reveal the possibility of using transcript levels of lipid metabolism-related genes in PBMCs as early biomarkers of metabolic health status.
Skeletal muscle Nr4a1 hypomethylation and gene induction reduce insulin sensitivity in sedentary maternal high-fat offspring
Schothorst, E.M. van; Schumann, Sara ; Stelt, I. van der; Dartel, D.A.M. van; Klaus, Susanne - \ 2018
Mus musculus - GSE104029 - PRJNA408062
Maternal high-fat consumption has negative effects on the offspring’s obesity/diabetes susceptibility and we hypothesize that epigenetic modifications in the skeletal muscle are partly responsible for this phenotype. To detect genes affected by maternal nutrition, offspring of low-fat (LF) and high-fat (HF) diet fed dams (C57BL/6 mice) received LF diet upon weaning and were sacrificed at an age of 6 or 25 weeks. M. quadriceps gene expression was investigated by microarray analysis revealing upregulation of the nuclear receptor Nr4a1 by maternal HF feeding. This was accompanied by promoter hypomethylation of CpG‑1408 which correlated with higher Nr4a1 gene expression at both ages. Offspring voluntary exercise training normalized Nr4a1 methylation/expression and ameliorated the negative effects of maternal HF feeding on insulin sensitivity. Overall, Nr4a1 expression correlated with higher insulin levels during oral glucose tolerance test and could, therefore, be involved in the programming offspring’s diabetes susceptibility.
A constraint-based model analysis of enterocyte mitochondrial adaptation to dietary interventions of lipid type and lipid load
Sinha, Neeraj ; Suarez-Diez, Maria ; Hooiveld, Guido J.E.J. ; Keijer, Jaap ; Santos, Vitor Martin dos; Schothorst, Evert M. van - \ 2018
Frontiers in Physiology 9 (2018). - ISSN 1664-042X
Constraint-based metabolic model - Enterocytes - High fat diet - Mitochondria - Mitochondrial dynamics - Omega-3 lipids

Computational modeling of mitochondrial adaptability and flexibility in the small intestine upon different nutritional exposures will provide insights that will help to define healthy diet interventions. Therefore, a murine enterocyte-specific mitochondrial constraint-based metabolic model (named MT_mmuENT127) was constructed and used to simulate mitochondrial behavior under different dietary conditions, representing various levels and composition of nutrients absorbed by the enterocytes in mice, primarily focusing on metabolic pathways. Our simulations predicted that increasing the fraction of marine fatty acids in the diet, or increasing the dietary lipid/carbohydrate ratio resulted in (i) an increase in mitochondrial fatty acid beta oxidation, and (ii) changes in only a limited subset of mitochondrial reactions, which appeared to be independent of gene expression regulation. Moreover, transcript levels of mitochondrial proteins suggested unaltered fusion-fission dynamics by an increased lipid/carbohydrates ratio or by increased fractions of marine fatty acids. In conclusion, our enterocytic mitochondrial constraint-based model was shown to be a suitable platform to investigate effects of dietary interventions on mitochondrial adaptation and provided novel and deeper insights in mitochondrial metabolism and regulation.

Weinig verschil in verteerbaarheid
Star, Laura ; Kwakernaak, Cees ; Mens, Annemarie ; Krimpen, Marinus van - \ 2018
De Pluimveehouderij 2018 (2018)6. - ISSN 0166-8250 - p. 22 - 24.
De verteringscoëfficiënten van gangbare grondstoffen voor leghennen kunnen ook worden toegepast op biologische grondstoffen. In onderzoek vonden Schothorst Feed Research en Wageningen Livestock Research slechts kleine verschillen in de verteerbaarheid van fosfor, stikstof en omzetbare energie tussen biologische en gangbare mengvoergrondstoffen voor leghennen.
Insulin sensitivity linked skeletal muscle Nr4a1 DNA methylation is programmed by the maternal diet and modulated by voluntary exercise in mice
Kasch, Juliane ; Kanzleiter, Isabel ; Saussenthaler, Sophie ; Schürmann, Annette ; Keijer, Jaap ; Schothorst, Evert van; Klaus, Susanne ; Schumann, Sara - \ 2018
Journal of Nutritional Biochemistry 57 (2018). - ISSN 0955-2863 - p. 86 - 92.
Perinatal maternal high-fat consumption is known to increase the obesity and type 2 diabetes susceptibility and to impair exercise performance in the offspring. We hypothesize that epigenetic modifications in the skeletal muscle are partly responsible for this phenotype. To detect skeletal muscle genes affected by maternal nutrition, male offspring of low-fat (LF) and high-fat (HF) diet fed dams (BL6 mice) received LF diet upon weaning and were sacrificed at 6 or 25 weeks of age. Gene expression of Musculus quadriceps was investigated by microarray analysis revealing an up-regulation of the nuclear receptor Nr4a1 by maternal HF feeding. This was accompanied by promoter hypomethylation of CpG-1408 which correlated with increased Nr4a1 gene expression at both ages. Offspring voluntary exercise training (by supplying running wheels from 7 to 25 weeks of age) normalized Nr4a1 methylation and gene expression respectively, and ameliorated the negative effects of maternal HF feeding on insulin sensitivity. Overall, Nr4a1 gene expression in skeletal muscle correlated with higher insulin levels during an oral glucose tolerance test and could, therefore, be involved in programming type 2 diabetes susceptibility in offspring exposed to perinatal high fat diet.
Fosforverteringscoefficienten grondstoffen
Mens, A.J.W. ; Jonge, L.H. de; Wikselaar, P.G. van; Bikker, P. ; Krimpen, M.M. van - \ 2018
V-focus 15 (2018)1. - ISSN 1574-1575 - 4 p.
In een onderzoek naar de voederwaarde en diervoedergrondstoffen, uitgevoerd door Wageningen Livestock Research in samenwerkin met Schothorst Feed Research, zijn een grote verschillen in verteerbaarheid van nutriënten gevonden tussen biologisch gehouden varkens te verbeteren kan intrinsiek fytase uit tarwe en tarwegries een uitkomst bieden.
No Adverse Programming by Post-Weaning Dietary Fructose of Body Weight, Adiposity, Glucose Tolerance, or Metabolic Flexibility
Bouwman, Lianne M.S. ; Fernández-Calleja, José M.S. ; Swarts, Hans J.M. ; Stelt, Inge van der; Oosting, Annemarie ; Keijer, Jaap ; Schothorst, Evert M. van - \ 2018
Molecular Nutrition & Food Research 62 (2018)2. - ISSN 1613-4125
carbohydrates - indirect calorimetry - metabolic programming - metabolism - monosaccharides
Scope: Metabolic programming can occur not only in the perinatal period, but also post-weaning. This study aims to assess whether fructose, in comparison to glucose, in the post-weaning diet programs body weight, adiposity, glucose tolerance, metabolic flexibility, and health at adult age. Methods and results: Three-week-old male and female C57BL6/JRccHsd mice are given an intervention diet with 32 energy percent (en%) glucose or fructose for only 3 weeks. Next, all animals are switched to the same 40 en% high fat diet for 9 weeks. Neither body weight nor adiposity differs significantly between the animals fed with glucose or fructose diets at any point during the study in both sexes. Glucose tolerance in adulthood is not affected by the post-weaning diet, nor are activity, energy expenditure, and metabolic flexibility, as measured by indirect calorimetry. At the end of the study, only in females fasting serum insulin levels and HOMA-IR index are lower in post-weaning fructose versus glucose diet (p = 0.02), without differences in pancreatic β-cell mass. Conclusions: Our present findings indicate no adverse programming of body weight, adiposity, glucose tolerance, and metabolic flexibility by dietary (solid) fructose in comparison to glucose in the post-weaning diet in mice.
Effect of dietary lipid structure in early life improves metabolic function of adult adipose tissue in mice
Baars, Annemarie ; Schipper, Lidewij ; Mischke, M. ; Klein Hazebroek, Marlou ; Schothorst, E.M. van; Beek, Eline van der; Oosting, Annemarie - \ 2017
Journal of Developmental Origins of Health and Disease 8 (2017)Supplement 1. - ISSN 2040-1744 - p. S52 - S53.
Monosaccharides in post-weaning diet of young mice program body composition and feeding behaviour in adulthood
Fernández Calleja, J.M.S. ; Bouwman, L.M.S. ; Swarts, J.J.M. ; Oosting, Annemarie ; Keijer, J. ; Schothorst, E.M. van - \ 2017
Journal of Developmental Origins of Health and Disease 8 (2017)Supplement 1. - ISSN 2040-1744 - p. S369 - S369.
Metabolic homeostasis in the cold requires neither FGF21 nor UCP1
Keipert, Susanne ; Kutschke, Maria ; Ost, Mario ; Schwarzmayr, Thomas ; Schothorst, E.M. van; Lamp, Daniel ; Brachthäuser, Laura ; Graf Pannatier, Elisabeth ; Neff, Frauke ; Jastroch, Martin - \ 2017
Transcriptomic analysis reveals a strong down regulation of immune response pathways in ferret aortic perivascular adipose tissue by cold exposure: potential implications of cardiovascular health
Reynés, Bàrbara ; Schothorst, E.M. van; García-Ruiz, Estefanía ; Keijer, J. ; Palou, Andreu ; Oliver, Paula - \ 2017
No adverse post-weaning programming by fructose of body weight, adiposity, glucose tolerance and metabolic flexilibility
Bouwman, L.M.S. ; Fernández Calleja, J.M.S. ; Keijer, J. ; Oosting, A. ; Schothorst, E.M. van - \ 2017
Nutritional effects by beta-carotene in lung in males and females of control mice versus BCMO knockout mice
Schothorst, E.M. van; Helden, Y.G.J. ; Keijer, J. ; Bunschoten, J.E. ; Lintig, J. von; Lietz, G. - \ 2017
Mus musculus - GSE98845 - PRJNA386385
Molecular mechanisms triggered by high dietary beta-carotene (BC) intake in lung are largely unknown. We performed microarray gene expression analysis on lung tissue of BC supplemented beta-carotene 15,150-monooxygenase 1 knockout (Bcmo1-/-) mice, which are—like humans—able to accumulate BC. Our main observation was that the genes were regulated in an opposite direction in male and female Bcmo1-/- mice by BC. The steroid biosynthetic pathway was overrepresented in BC-supplemented male Bcmo1-/- mice. Testosterone levels were higher after BC supplementation only in Bcmo1-/- mice, which had, unlike wild-type (Bcmo1+/+) mice, large variations. We hypothesize that BC possibly affects hormone synthesis or metabolism. Since sex hormones influence lung cancer risk, these data might contribute to an explanation for the previously found increased lung cancer risk after BC supplementation (ATBC and CARET studies). Moreover, effects of BC may depend on the presence of frequent human BCMO1 polymorphisms, since these effects were not found in wild-type mice.
Nutritional effects by beta-carotene in liver in males and females of control wildtype mice versus BCMO knockout mice
Schothorst, E.M. van; Helden, Y.G.J. ; Keijer, J. ; Hessel, S. ; Bunschoten, J.E. ; Lintig, J. von; Lietz, G. - \ 2017
Mus musculus - GSE98846 - PRJNA386384
Molecular mechanisms triggered by high dietary beta-carotene (BC) intake in liver are largely unknown. We performed microarray gene expression analysis on liver tissue of BC supplemented beta-carotene 15,150-monooxygenase 1 knockout (Bcmo1-/-) mice, which are—like humans—able to accumulate BC. This was compared with litter mates being wild-type (Bcmo1+/+) mice, and we analysed both males and females, as we previously showed that in lung tissue we observed opposite gene regulation between males and females.
Nutritional effects by beta-carotene versus control in lung, inguinal white adipose tissue, and liver in males and females of control wildtype mice versus BCMO/BCO1 knockout mice
Schothorst, E.M. van - \ 2017
Mus musculus - GSE98847 - PRJNA386383
This SuperSeries is composed of the SubSeries listed below.
Identification of blood cell transcript levels of Promc and Agrp in the offspring of gestational calorie restricted rats as potential biomarkers of predisposition to impaired energy homeostasis control
Szostaczuk, N. ; Schothorst, E.M. van; Sánchez, J. ; Priego, T. ; Bekkenkamp-Grovenstein, M. ; Keijer, J. ; Palou, A. ; Picó, C. - \ 2017
- 2 p.
Monosaccharides in post-weaning diet of young mice program body composition and feeding behaviour in adulthood
Fernández Calleja, J.M.S. ; Bouwman, L.M.S. ; Swarts, J.J.M. ; Oosting, A. ; Keijer, J. ; Schothorst, E.M. van - \ 2017
- 2 p.
No adverse metabolic programming of dietary fructose versus glucose post-weaning by extensive basal and challenged phenotyping in mice
Schothorst, E.M. van; Bouwman, L.M.S. ; Fernández Calleja, J.M.S. ; Swarts, J.J.M. ; Oosting, A. ; Keijer, J. - \ 2017
Check title to add to marked list
<< previous | next >>

Show 20 50 100 records per page

 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.