Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Analysis and modeling of enhanced green fluorescent protein diffusivity in whey protein gels
Luo, Qi ; Sewalt, Erik ; Borst, Jan Willem ; Westphal, Adrie H. ; Boom, Remko M. ; Janssen, Anja E.M. - \ 2019
Food Research International 120 (2019). - ISSN 0963-9969 - p. 449 - 455.
Diffusion - FCS - Gastric digestion - GFP - Modeling - Pepsin - Whey protein gel

During gastric digestion, hydrolysis of proteins by pepsin contributes largely to the breakdown of protein-rich food. We hypothesized that the effect of pepsin is limited by its diffusivity, which is co-determined by the food structure and the local pH in the food during digestion. To investigate the principle mechanism of enzyme diffusion in food matrices, we used enhanced green fluorescent protein (EGFP) as probe to study the diffusivity of proteins in whey protein isolate gels, using fluorescence correlation spectroscopy (FCS). Gels made with different ionic strength showed distinctive elastic moduli but did not show differences in diffusivity of EGFP. Some models for diffusion in hydrogels yield good description of the obtained data, and can approximate the enzyme diffusion in diverse food matrices. However, the enzyme pepsin is more complicated than the probe EGFP, to yield more accurate predictions, electrostatic and enzyme-substrate interaction also need to be considered.

Duurzaam en gezond voedsel op basis van onderzoek aan minuscule druppels in emulsies
Berton-Carabin, Claire ; Schroder, Anja ; Sewalt, Jos - \ 2018
Constant flux microfiltration with sieves with uniform pores
Drijer, I. ; Laar, T.X. van de; Fadhilatunnur, H. ; Tzompa Sosa, D.A. ; Sewalt, J.J.W. ; Valenberg, H.J.F. van; Schroen, C.G.P.H. - \ 2016
Continuous purification of galacto-oligosaccharide mixtures by using cascaded membrane filtration
Patil, Nirmal V. ; Schotel, Thom ; Rodríguez Gómez, Carol V. ; Aguirre Montesdeoca, Victor ; Sewalt, Jos J.W. ; Janssen, Anja E.M. ; Boom, Remko M. - \ 2016
Journal of Chemical Technology and Biotechnology 91 (2016)5. - ISSN 0268-2575 - p. 1478 - 1484.
Continuous purification - Downstream processing - Filtration - GOS - Membrane cascade - Spiral wound membrane

BACKGROUND: Membrane filtration has the potential for purification of oligosaccharide mixtures. However, single stage performance is limited by the relatively low yield and purity that can be achieved. This paper explores the potential of a three-stage pilot-scale spiral wound membrane filtration cascade for the removal of monosaccharides and disaccharides from a galacto-oligosaccharide mixture. RESULTS: Two three-stage cascaded configurations with GH membranes were tested, one ideal-like configuration (cascade 1) and one adapted configuration (cascade 2). Cascade 1 performed well with regard to oligosaccharide yield (e.g. yield 84%, purity 0.46), while cascade 2 performed better in terms of oligosaccharide purity (e.g. yield 62%, purity 0.58). This might be compared with conventional single stage concentration which resulted in an oligosaccharide yield of 56% with a purity of 0.53. In addition these cascade configurations were explored using different membranes (GE and GK membranes) in each stage. CONCLUSION: The yield and purity in the cascaded systems were higher than the yield and purity of a single stage system with volume reduction. The yield and purity can be further enhanced by using an inhomogeneous cascade with different membranes in each stage.

Separation of an inulin mixture using cascaded nanofiltration
Patil, N.V. ; Feng, Xiaoxia ; Sewalt, J.J.W. ; Boom, R.M. ; Janssen, A.E.M. - \ 2015
Separation and Purification Technology 146 (2015). - ISSN 1383-5866 - p. 261 - 267.
Continuous purification - Downstream processing - Filtration - Inulin - Membrane cascade - Spiral wound membrane

This paper examines the use of a pilot-scale spiral wound nanofiltration cascade with regard to separation of mono- and disaccharides from a mixture of inulin of different polymer sizes. The choices of the membrane and operational conditions were based on single stage experiments. Two 3-stage cascade configurations with GE membranes were tested, a counter-current product recycle cascade (configuration 1) and an adapted cascade (configuration 2). The results show that the performance of the cascaded systems is significantly improved as compared to conventional single stage concentration and diafiltration. The yield increased from 77% in a conventional single stage system, to 89% in a single stage diafiltration system, to 99% in the cascaded systems. The yield is improved without any loss in purity. The purity of the inulin mixture was in the range of 94-96%.

Business cases Duurzame Greenport Westland-Oostland : managementsamenvatting
Ruijs, M.N.A. ; Sewalt, K. ; Berg, G.J. van den; Janssen, E. ; Poot, E.H. - \ 2014
Bleiswijk : Wageningen UR Glastuinbouw (Rapport / Wageningen UR Glastuinbouw 1318) - 22
landbouwregio's - regionaal beleid - glastuinbouw - kennisoverdracht - warmteuitwisseling - geothermische energie - afvalwaterbehandeling - integraal waterbeheer - economische modellen - energiegebruik - onderzoeksprojecten - agricultural regions - regional policy - greenhouse horticulture - knowledge transfer - heat exchange - geothermal energy - waste water treatment - integrated water management - economic models - energy consumption - research projects
Het programma Taskforce Duurzame Greenport Westland-Oostland heeft als doel het aanjagen van regionale duurzaamheidsprojecten in de Greenport Westland-Oostland. In het project Kennisoverdracht zijn zeven van dergelijke projecten als business case beschreven, die raakvlak hebben met de milieuthema’s energie of water.
Allow us to trigger your curiosity!
Kassa, S.B. ; Dijke, K.C. van; Kulrattanarak, T. ; Rossier Miranda, F.J. ; Rosso, M. ; Sawalha, H.I.M. ; Steegmans, M.L.J. ; Zwan, E.A. van der; Schroën, C.G.P.H. ; Sewalt, J.J.W. ; Sman, R.G.M. van der; Boom, R.M. - \ 2008
Enzymatic synthesis of food additives
Padt, A. van der; Boon, F. ; Heinsman, N.W.J.T. ; Sewalt, J. ; Riet, K. van 't - \ 2000
In: Trends in Food Engineering / Lozano, J.E., Lancaster, Basel : Technomic (Food Preservation Technology Series ) - ISBN 9781566769914 - p. 299 - 309.
Potential effect of the membrane potting on the long-term performance during hydrolysis of edible oil
Sewalt, J.J.W. ; Padt, A. van der; Riet, K. van 't - \ 2000
Journal of Membrane Science 164 (2000). - ISSN 0376-7388 - p. 79 - 88.
The nature of fatty acid modifies the equilibrium position in the esterification catalyzed by lipase
Flores, M.V. ; Sewalt, J.J.W. ; Janssen, A.E.M. ; Padt, A. van der - \ 2000
Biotechnology and Bioengineering 67 (2000)3. - ISSN 0006-3592 - p. 364 - 371.
The equilibrium position in lipase mediated esterification of various fatty acids and butanol was studied. The influence of the chain length and the presence of unsaturations in the fatty acids on the equilibrium position was measured and predicted. To predict equilibrium position the program TREP extended (TREPEX) based on the UNIFAC group contribution method was used. Using an equilibrium constant of 35, calculated on the basis of thermodynamic activities, the equilibrium position between butanol and saturated and/or unsaturated fatty acids with different chain lengths can be predicted. The ester mole fraction at equilibrium increases with the fatty acid chain length, and for fatty acids with the same carbon number, the highest values are found for unsaturated fatty acids. For reaction systems containing two saturated fatty acids, a slightly higher mole fraction is obtained for the fatty acid with the higher chain length, while for mixtures consisting of saturated and unsaturated fatty acids, the mole fractions of the unsaturated esters are lower than those of the saturated ones, regardless the chain length of the fatty acid. These experimental results are in good agreement with the calculations with TREPEX
Membrane bioreactor design to force equilibrium towards a favourable product yield.
Padt, A. van der; Sewalt, J.J.W. ; Riet, K. van 't - \ 1996
In: Engineering of/with lipases / Malcata, F.X., Dordrecht : Kluwer Academic Publishers - p. 473 - 481.
Lipase membrane bioreactors to produce pure triglycerides.
Padt, A. van der; Sewalt, J.J.W. ; Riet, K. van 't - \ 1993
In: Abstracts 6th Int. Congr. Engineering and Food, Chiba Japan - p. 192 - 192.
Two-phase membrane bioreactor.
Padt, A. van der; Schroën, C.G.P.H. ; Sewalt, J. ; Riet, K. van 't - \ 1993
In: Posterdag NMG, Membraantechnologie 7 - p. 45 - 45.
On-line water removal during enzymatic triacylglycerol synthesis by means of pervaporation.
Padt, A. van der; Sewalt, J.J.W. ; Riet, K. van 't - \ 1993
Journal of Membrane Science 80 (1993). - ISSN 0376-7388 - p. 199 - 208.
Acylglycerol equilibrium in a two-phase system.
Padt, A. van der; Janssen, A.E.M. ; Sewalt, J.J.W. ; Riet, K. van 't - \ 1993
Biocatalysis 7 (1993). - ISSN 0886-4454 - p. 267 - 277.
Enzymatic synthesis of monoglycerides in a membrane bioreactor with an in-line adsorption column.
Padt, A. van der; Keurentjes, J.T.F. ; Sewalt, J.J.W. ; Dam, E.M. van; Dorp, L.J. van; Riet, K. van 't - \ 1992
Journal of the American Oil Chemists Society 69 (1992). - ISSN 0003-021X - p. 748 - 754.
Candida rugosa lipase stability during acylglycerol synthesis.
Padt, A. van der; Sewalt, J.J.W. ; Agoston, S.M.I. ; Riet, K. van 't - \ 1992
Enzyme and Microbial Technology 14 (1992). - ISSN 0141-0229 - p. 805 - 812.
Synthesis of triacylglycerols. The crucial role of water activity control.
Padt, A. van der; Sewalt, J.J.W. ; Riet, K. van 't - \ 1992
In: Biocatalysis in non-conventional media / Tramper, J., Elsevier Sci. Publ - p. 557 - 562.
Scaling-up of lipase-catalyzed hydrolysis of triglycerides in a membrane bioreactor .
Sewalt, J.J.W. ; Padt, A. van der; Riet, K. van 't - \ 1990
Membraan Technologie 5 (1990). - ISSN 0923-2052 - p. 48 - 48.
Enzymatic acylglycerol synthesis in a membrane reactor.
Padt, A. van der; Edema, M.J. ; Sewalt, J.J.W. ; Riet, K. van 't - \ 1990
Journal of the American Oil Chemists Society 67 (1990). - ISSN 0003-021X - p. 347 - 352.
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