Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Quiescent center initiation in the Arabidopsis lateral root primordia is dependent on the SCARECROW transcription factor
Goh, Tatsuaki ; Toyokura, Koichi ; Wells, Darren M. ; Swarup, Kamal ; Yamamoto, Mayuko ; Mimura, Tetsuro ; Weijers, Dolf ; Fukaki, Hidehiro ; Laplaze, Laurent ; Bennett, Malcolm J. ; Guyomarc’h, Soazig - \ 2016
Development 143 (2016)18. - ISSN 0950-1991 - p. 3363 - 3371.
3D live imaging - Arabidopsis thaliana - Lateral root organogenesis - Quiescent center - SCARECROW

Lateral root formation is an important determinant of root system architecture. In Arabidopsis, lateral roots originate from pericycle cells, which undergo a program of morphogenesis to generate a new lateral root meristem. Despite its importance for root meristem organization, the onset of quiescent center (QC) formation during lateral root morphogenesis remains unclear. Here, we used live 3D confocal imaging to monitor cell organization and identity acquisition during lateral root development. Our dynamic observations revealed an early morphogenesis phase and a late meristem formation phase as proposed in the bi-phasic growth model. Establishment of lateral root QCs coincided with this developmental phase transition. QC precursor cells originated from the outer layer of stage II lateral root primordia, within which the SCARECROW (SCR) transcription factor was specifically expressed. Disrupting SCR function abolished periclinal divisions in this lateral root primordia cell layer and perturbed the formation of QC precursor cells. We conclude that de novo QC establishment in lateral root primordia operates via SCR-mediated formative cell division and coincides with the developmental phase transition.

Plant embryogenesis requires AUX/LAX-mediated auxin influx
Robert, H.S. ; Grunewald, W. ; Cannoot, B. ; Soriano, M. ; Swarup, R. ; Weijers, D. ; Bennett, M. ; Boutilier, K.A. ; Friml, J. - \ 2015
Development 142 (2015). - ISSN 0950-1991 - p. 702 - 711.
arabidopsis root apex - apical-basal axis - tobacco cells - permease aux1 - gene activity - transport - embryo - monopteros - proteins - carrier
The plant hormone auxin and its directional transport are known to play a crucial role in defining the embryonic axis and subsequent development of the body plan. Although the role of PIN auxin efflux transporters has been clearly assigned during embryonic shoot and root specification, the role of the auxin influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here, we used chemical and genetic tools on Brassica napus microspore-derived embryos and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and LAX2 are required for both shoot and root pole formation, in concert with PIN efflux carriers. Furthermore, we uncovered a positive-feedback loop betweenMONOPTEROS(ARF5)- dependent auxin signalling and auxin transport. ThisMONOPTEROSdependent transcriptional regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4) carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip. These results indicate that auxin-dependent cell specification during embryo development requires balanced auxin transport involving both influx and efflux mechanisms, and that this transport is maintained by a positive transcriptional feedback on auxin signalling.
Cytokinins act directly on lateral root founder cells to inhibit root initiation
Laplaze, L. ; Benkova, E. ; Casimiro, I. ; Maes, L. ; Vanneste, S. ; Swarup, R. ; Weijers, D. ; Calvo, V. ; Parizot, B. ; Herrera-Rodriguez, M.B. ; Offringa, R. ; Graham, N. ; Doumas, P. ; Friml, J. ; Bogusz, D. ; Beeckman, T. ; Bennett, M. - \ 2007
The Plant Cell 19 (2007)12. - ISSN 1040-4651 - p. 3889 - 3900.
arabidopsis-thaliana - response regulators - tobacco plants - pea roots - auxin - shoot - gene - expression - pericycle - meristem
In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.
Apical/basal polarity : why plant cells do not stand on their heads
Friml, J. ; Benfey, P. ; Benková, E. ; Bennett, M. ; Berleth, T. ; Geldner, N. ; Grebe, M. ; Hejátko, J. ; Jürgens, G. ; Laux, T. ; Lindsey, K. ; Lukowitz, W. ; Luschnig, C. ; Offringa, R. ; Scheres, B.J.G. ; Swarup, R. ; Torres-Ruiz, R. ; Weijers, D. ; Zazímalová, E. - \ 2006
Trends in Plant Science 11 (2006)1. - ISSN 1360-1385 - p. 12 - 14.
dependent auxin gradients - arabidopsis - efflux
Natural allelic variation identifies new genes in the Arabidopsis circadian system
Swarup, K. ; Alonso-Blanco, C. ; Lynn, J.R. ; Michaels, S.D. ; Amasino, R.M. ; Koornneef, M. ; Millar, A.J. - \ 1999
The Plant Journal 20 (1999). - ISSN 0960-7412 - p. 67 - 78.
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