- A. Bossers (3)
- J.C. Espinosa (1)
- C. Fast (3)
- I. Gerhauser (1)
- W. Goldmann (2)
- L.G.M. Gorris (1)
- M.H. Groschup (2)
- Martin H. Groschup (1)
- V. Haist (1)
- N. Hunter (1)
- J.G. Jacobs (1)
- J.P.M. Langeveld (2)
- J. Langeveld (1)
- I. Lantier (1)
- F. Lantier (1)
- Nadeem Muhammad (1)
- M. Nadeem (1)
- P. Papasavva-Stylianou (1)
- K. Rohn (1)
- C. Rossignol (1)
- I. Spitzbarth (1)
- Kerstin Tauscher (2)
- B. Tauscher (1)
- K. Tauscher (1)
Protecting effect of PrP codons M142 and K222 in goats orally challenged with bovine spongiform encephalopathy prions
Fast, C. ; Goldmann, W. ; Berthon, P. ; Tauscher, Kerstin ; Andréoletti, O. ; Lantier, I. ; Rossignol, C. ; Bossers, A. ; Jacobs, J.G. ; Hunter, N. ; Groschup, Martin H. ; Lantier, F. ; Langeveld, J.P.M. - \ 2017
Veterinary Research 48 (2017)1. - ISSN 0928-4249
Breeding towards genetic resistance to prion disease is effective in eliminating scrapie. In sheep, classical forms of scrapie have been eradicated almost completely in several countries by breeding programs using a prion protein (PrP) gene (PRNP) amino acid polymorphism. For goats, field and experimental studies have provided evidence for several amino acid polymorphisms that are associated with resistance to scrapie, but only limited data are available concerning the susceptibility of caprine PRNP genotypes to BSE. In this study, goat kids representing five PRNP genotypes based on three polymorphisms (M142, Q211 and K222 and the wild type I142, R211 and Q222) were orally challenged with bovine or goat BSE. Wild type goats were killed with clinical signs between 24-28 months post inoculation (mpi) to both challenges, and goats with genotype R/Q211 succumbed between 29-36 mpi. I/M142 goats developed clinical signs at 44-45 mpi and M/M142 goats remained healthy until euthanasia at 48 mpi. None of the Q/K222 goats showed definite clinical signs. Taken together the highest attack ratios were seen in wild type and R/Q211 goats, and the lowest in I/M142, M/M142 and Q/K222. In all genotype groups, one or more goats remained healthy within the incubation period in both challenges and without detectable PrP deposition in the tissues. Our data show that both the K222 and M142 polymorphisms lengthen the incubation period significantly compared to wild type animals, but only K222 was associated with a significant increase in resistance to BSE infection after oral exposure to both BSE sources.
Immunolabelling of non-phosphorylated neurofilament indicates damage of spinal cord axons in TSE-infected goats
Nadeem, M. ; Spitzbarth, I. ; Haist, V. ; Rohn, K. ; Tauscher, K. ; Rohn, K. ; Bossers, A. ; Langeveld, J. ; Papasavva-Stylianou, P. ; Groschup, M.H. ; Baumgärtner, W. ; Gerhauser, I. ; Fast, C. - \ 2016
Veterinary Record 178 (2016)6. - ISSN 0042-4900 - p. 141 - 141.
Effect of Q211 and K222 PRNP polymorphic variants in the susceptibility of goats to oral infections with Goat Bovine Spongiform Encephalopathy
Aguilar-Calvo, Patricia ; Fast, C. ; Tauscher, Kerstin ; Espinosa, J.C. ; Groschup, M.H. ; Muhammad, Nadeem ; Goldmann, W. ; Langeveld, J.P.M. ; Bossers, A. ; Andreoletti, O. - \ 2015
The Journal of Infectious Diseases 212 (2015)4. - ISSN 0022-1899 - p. 664 - 672.
Background. The prion protein-encoding gene (PRNP) is one of the major determinants for scrapie occurrence in sheep and goats. However, its effect on bovine spongiform encephalopathy (BSE) transmission to goats is not clear.
Methods. Goats harboring wild-type, R/Q211 or Q/K222 PRNP genotypes were orally inoculated with a goat-BSE isolate to assess their relative susceptibility to BSE infection. Goats were killed at different time points during the incubation period and after the onset of clinical signs, and their brains as well as several peripheral tissues were analyzed for the accumulation of pathological prion protein (PrPSc) and prion infectivity by mouse bioassay.
Results. R/Q211 goats displayed delayed clinical signs compared with wild-type goats. Deposits of PrPSc were detected only in brain, whereas infectivity was present in peripheral tissues too. In contrast, none of the Q/K222 goats showed any evidence of clinical prion disease. No PrPSc accumulation was observed in their brains or peripheral tissues, but very low infectivity was detected in some tissues very long after inoculation (44–45 months).
Conclusions. These results demonstrate that transmission of goat BSE is genotype dependent, and they highlight the pivotal protective effect of the K222 PRNP variant in the oral susceptibility of goats to BSE.
|Quality and safety aspects of novel minimal processing techniques
Gorris, L.G.M. ; Tauscher, B. - \ 1999
In: Processing foods : quality optimization and process assessment / Oliviera, F.A.R., Oliviera, J.C., Boca Raton : CRC Press