Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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An improved vitrification protocol for equine immature oocytes, resulting in a first live foal
Ortiz-Escribano, N. ; Bogado Pascottini, O. ; Woelders, H. ; Vandenberghe, L. ; Schauwer, C. De; Govaere, J. ; Abbeel, E. Van den; Vullers, T. ; Ververs, C. ; Roels, K. ; De Velde, M. Van; Soom, A. van; Smits, K. - \ 2018
Equine Veterinary Journal 50 (2018)3. - ISSN 0425-1644 - p. 391 - 397.
Cumulus cells - Horse - Oocytes - Vitrification
Background: The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. Objectives: The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. Study design: Experimental in vitro and in vivo trials. Methods: Immature equine oocytes were vitrified after a short exposure to high concentrations of cryoprotective agents (CPAs), or a long exposure to lower concentrations of CPAs. In Experiment 1, the maturation of oocytes surrounded by multiple layers of cumulus cells (CC oocytes) and oocytes surrounded by only corona radiata (CR oocytes) was investigated. In Experiment 2, spindle configuration was determined for CR oocytes vitrified using the two vitrification protocols. In Experiment 3, further embryonic development was studied after fertilisation and culture. Embryo transfer was performed in a standard manner. Results: Similar nuclear maturation rates were observed for CR oocytes vitrified using the long exposure and nonvitrified controls. Furthermore, a lower maturation rate was obtained for CC oocytes vitrified with the short exposure compared to control CR oocytes (P = 0.001). Both vitrification protocols resulted in significantly higher rates of aberrant spindle configuration than the control groups (P<0.05). Blastocyst development only occurred in CR oocytes vitrified using the short vitrification protocol, and even though blastocyst rates were significantly lower than in the control group (P<0.001), transfer of five embryos resulted in one healthy foal. Main limitations: The relatively low number of equine oocytes and embryo transfer procedures performed. Conclusions: For vitrification of immature equine oocytes, the use of 1) CR oocytes, 2) a high concentration of CPAs, and 3) a short exposure time may be key factors for maintaining developmental competence.
VIP Praktijknetwerk Netinnovatie Zuid
Marlen, B. van; Rink, G.J. ; Vandenberghe, C. - \ 2016
IJmuiden : IMARES (Rapport / IMARES C147/14) - 77 p.
visserij - duurzaamheid (sustainability) - pleuronectiformes - netten - noordzee - fisheries - sustainability - nets - north sea
Het VIP project Praktijknetwerk Netinnovatie Zuid beoogde een bijdrage te leveren aan een meer duurzame kottervisserij op platvis op zuidelijke bestekken in de Noordzee door het ontwikkelen van nieuwe netten met een lager brandstofverbruik, en minder netslijtage, bijvangsten en discards.
Specificity of anti-pru av 1 antibodies for the detection of pru av 1 isoallergens
Verschuren, M.C.M. ; Bastiaan-Net, S. ; Uden, N.M.E. van; Vandenberghe, K. ; Foetisch, K. ; Scheurer, S. ; Wichers, H.J. ; Noij, T. - \ 2015
VIP project: Passieve Visserij Ontwikkeling
Marlen, B. van; Vandenberghe, C. ; Craeynest, N. ; Korving, A. ; Cramer, R. ; Reker, E. - \ 2011
IJmuiden : IMARES Wageningen UR (Rapport / IMARES Wageningen UR C117/11)
visserij - vistuig - vismethoden - alternatieve methoden - netten - fisheries - fishing gear - fishing methods - alternative methods - nets
De introductie van passieve vistechnieken vormt een onderdeel van duurzame visserij ontwikkeling. Drie vaartuigen (KW-2, SCH-61 en IJM-27) beoefenen al geruime tijd passieve technieken. In het kader van dit project werd kennisoverdracht gedaan, vooral vanuit ILVO-Vlaanderen (Visserij Oostende, België), waar recent ervaring is opgedaan met schakels en potten, maar ook vanuit Denemarken, Frankrijk en Groot Brittannië. Na deze fase zijn de drie schippers gaan werken met verschillende passieve technieken (staand want, jiggen en potten) en hebben hiermee praktijkervaring opgedaan. Vervolgens werden vangsten en bijvangsten bemonsterd door onderzoekers van het ILVO en zijn er een tijd lang vangstgegevens bijgehouden door de schippers en opgeleverd aan IMARES en statistisch bewerkt. Een economische analyse laat zien, dat investeringen binnen redelijke korte tijd (1-3 jaren) zijn terug te verdienen. Hoewel de aanlandingen vergeleken met de boomkor relatief laag zijn, zijn de discards ook veel geringer. Met het lage brandstofverbruik zouden passieve vistuigen voor een beperkt deel van de vloot een alternatief kunnen zijn.
Catch comparison of pulse trawls vessels and a tickler chain beam trawler
Marlen, B. van; Wiegerinck, J.A.M. ; Os-Koomen, E. van; Barneveld, E. van; Bol, R.A. ; Groeneveld, K. ; Nijman, R.R. ; Buyvoets, E. ; Vandenberghe, C. ; Vanhalst, K. - \ 2011
IJmuiden : IMARES (Report / IMARES Wageningen UR C122b/11) - 67
pulsvisserij - boomkorvisserij - visvangsten - vismethoden - discards - nederland - pulse trawling - beam trawling - fish catches - fishing methods - netherlands
Comparative fishing trials were conducted in May 2011 (week 19) on commercial beam trawlers fishing with conventional tickler chain beam trawls (on MFV GO4), pulse wings made by HFK-Engineering of Baarn, the Netherlands (MFV TX36), and pulse trawls produced by the DELMECO-Group of Goes, the Netherlands (version used on MFV TX68). The three vessels fished side-by-side as much as possible. Landings and discards of these vessels were monitored. Special emphasis was given on cod and whiting, that were dissected to study possible spinal damage. Result for TX36 and TX68 are expressed in terms of percentages of GO4. The pulse characteristics were as follows: TX36: voltage 45 V0 to peak, pulse frequency: 45 Hz, pulse duration 380 μs; electric power on single gear: 7.0 kW; TX68: voltage 50 V0 to peak, pulse frequency: 50 Hz, pulse duration 220 μs; electric power on single gear: 8.5 kW. The fuel consumption recorded over the whole week was considerably lower for the pulse trawls, i.e. on TX36 (40%) and on TX68 (54%), than for the tickler chain beam trawls used on the GO4. The net earnings (taken as gross earnings minus fuel costs) for the TX36 were almost twice as large at 186%, and for the TX68 also considerably higher at 155%. The vessels with pulse trawls caught fewer (65-69%) target species, but also less (30-50%) immature and non-target fish ('discards'), and benthic species (48-73%) than the vessel with tickler chains on these fishing grounds and in this period. The pulse gears caught fewer (19-42%) kg per hour cod than the tickler chain beam trawls, but the catches of cod on all three vessels were very small. For plaice and dab these differences were statistically proven, for brill, turbot and cod this was not the case. There was no marked difference between both pulse trawl vessels in total landings. The TX68 caught less marketable sole, but not significantly less undersized sole than the GO4. The TX36 caught less undersized sole, but here the difference in marketable fish was not significant. Catches of brill and turbot were so small that no statistically substantiated conclusion could be drawn. Only for undersized turbot the TX36 caught less. For whiting we found a demonstrable reduction in both marketable and undersized fish in both pulse fishing vessels. The TX36 caught less whiting in number per hour. The CPUEs found from the auction data and the sampled hauls correlated reasonably well for the most abundant species, such as plaice and sole. However, for less abundant species the results did not match very well, and care should be taken to increase the sampling rate in future comparative fishing studies. Spinal fracture in cod occurred under pulse stimulation but to a limited extent in both marketable and undersized fish. There is an indication that this happens slightly more on TX68 (11%) than on TX36 (7%). Whiting hardly seems to suffer any damage.
Vangstvergelijking tussen pulskorschepen en een conventioneel boomkor-vaartuig
Marlen, B. van; Wiegerinck, J.A.M. ; Os-Koomen, E. van; Barneveld, E. van; Bol, R.A. ; Groeneveld, K. ; Nijman, R.R. ; Buyvoets, E. ; Vandenberghe, C. ; Vanhalst, K. - \ 2011
IJmuiden : IMARES (Rapport / IMARES Wageningen UR C122a/11) - 65
pulsvisserij - zeevisserij - boomkorvisserij - vismethoden - visvangsten - pulse trawling - marine fisheries - beam trawling - fishing methods - fish catches
In mei 2011 (week 19) werd een vergelijkende visserij uitgevoerd aan boord van de kotters GO4 (wekkerboomkorren), TX36 (HFK pulswings), en de toenmalige versie van DELMECO pulskorren gebruikt op de TX68. De schepen visten zoveel mogelijk giek-aan-giek in hetzelfde gebied. Tevens werd aandacht besteed aan mogelijk ruggengraatletsel van kabeljauw en wijting. De pulskarakteristieken waren als volgt: TX36: voltage 45 V0 tot piek, pulsfrequentie: 45 Hz, pulsduur 380 µs; elektrisch vermogen op één tuig: 7.0 kW; TX68: voltage 50 V0 tot piek, pulsfrequentie: 50 Hz, pulsduur 220 µs; elektrisch vermogen op één tuig: 8.5 kW. Het brandstofverbruik over de gehele visweek was aanzienlijk minder voor de TX36 (40%) en de TX68 (54%), dan de GO4. Voor de netto besomming (= bruto besomming – gasoliekosten) vonden we zelfs bijna een verdubbeling in efficiëntie voor de TX36 (186%), en een ruime toename voor de TX68 (155%). De schepen met pulstuigen vingen minder (65-69%) van de belangrijkste doelsoorten dan het schip met wekkerkorren op dit bestek en in deze periode, maar ook minder (30-50%) ondermaatse en niet-vermarktbare soorten vis (EN: ‘discards’) en minder bodemdieren (48-73%). De pulstuigen bleken minder (19-42%) maatse kabeljauw in kg/u te vangen dan de wekkerkor, maar de vangsten van deze soort waren voor alle schepen gering. Voor schol en schar waren de verschillen statistisch aantoonbaar, voor griet, tarbot wijting en kabeljauw niet. De pulskorschepen onderling lieten geen duidelijke verschillen in totale aanlanding zien. De TX68 bleek minder maatse tong te vangen, maar niet duidelijk minder ondermaatse tong dan de GO4. Bij de TX36 was het andersom: hier werd minder ondermaatse tong gevangen, maar het verschil in maatse vis was niet duidelijk. De vangsten van griet en tarbot waren zo gering, dat doorgaans geen statistisch onderbouwde conclusie kon worden getrokken. Alleen van ondermaatse tarbot ving de TX36 significant minder. Voor wijting vonden we wel een aanwijsbare vermindering van zowel maatse als ondermaatse vis voor de pulskorschepen. In aantal/u bleek de TX36 ook minder te vangen. Er was een redelijke overeenkomst tussen het vangstsucces (vangst per eenheid van inspanning of CPUE) berekend uit de afslaggegevens en het vangstsucces berekend uit de bemonsterde trekken voor soorten die talrijk voorkomen, zoals schol en tong. Voor minder voorkomende soorten, was de correlatie minder sterk. Dit zou voorkomen kunnen worden door meer en grotere monsters te nemen in toekomstige vangstvergelijkingen. Kabeljauw (ondermaatse en maatse vis) ondervond in de pulstuigen in beperkte mate breuk van de ruggengraat. Er is een indicatie, dat dit voor de TX68 iets meer (11%) optrad dan voor de TX36 (7%). Wijting leek nauwelijks schade te ondervinden.
Ontwikkeling van de HydroRig
Marlen, B. van; Vandenberghe, C. ; Duren, L.A. ; Kleermaeker, G.H. ; Urk, R. van - \ 2011
IJmuiden : IMARES (Rapport / Wageningen IMARES C133/11) - 69
vistuig - visserij - visserijbeheer - vismethoden - pleuronectiformes - nederland - fishing gear - fisheries - fishery management - fishing methods - netherlands
De HydroRig is een alternatief vistuig voor de vangst van platvis ter vervanging van de wekkerstimulering in de boomkorvisserij. De noodzaak voor alternatieven komt voort uit ecosysteem kritiek op de boomkor met wekkers en de sterk oplopende brandstofkosten. In Nederland werden er al proeven gedaan aan een vistuig waarbij de boom is vervangen door een vleugel om de stroming op de bodem te beïnvloeden. Het idee van beïnvloeding van de stroming om vangst te verbeteren kwam oorspronkelijk uit de VS in een toepassing op een schelpdierkor. Door middel van bolkappen in het vistuig worden bodemdieren omhoog gedreven om beschikbaar te komen voor vangst. Dit rapport beschrijft de ontwikkeling vanaf 2008 in het VIP project HydroRig een geeft de stadia, van experimenten op zee met een aangepast vleugelprofiel, stromingsberekeningen en proeven in het laboratorium (water-grond goot) van DELTARES te Delft aan zgn. ‘bolkappen’, en toepassing hiervan op zee in verschillende configuraties met vangstmonitoring en onderwaterobservaties op de FD-281. Aan het eind van het project werden door middel van modelproeven in de ‘flume tank’ van IFREMER te Boulogne, Frankrijk nieuwe ontwerpen gemaakt voor een net met de onderpees dichter bij de boom. Gemiddeld werd bij vergelijking met het zusterschip FD-283, ook vissend op schol met een traditioneel boomkortuig en 100 mm maaswijdte, met de HydroRig (FD-281) ca. 21% brandstof bespaard, maar daartegenover stond een ca. 32% lagere besomming. Het vissen met de HydroRig in het commerciële bedrijf stagneert momenteel door de lage scholprijzen, die een belemmering vormen tot verder experimenteren. Toch wordt aanbevolen de proeven met bolkappen en nieuwe netontwerpen te vervolgen en te pogen de visnamigheid op schol te verbeteren, omdat de HydroRig veel minder benthos bijvangt.
Nurse plants, tree saplings and grazing pressure: changes in facilitation along a biotic environmental gradient
Smit, C. ; Vandenberghe, C. ; Ouden, J. den; Muller-Scharer, H. - \ 2007
Oecologia 152 (2007)2. - ISSN 0029-8549 - p. 265 - 273.
positive interactions - wooded pastures - associational resistance - arid environments - communities - competition - stress - regeneration - metaanalysis - survival
Current conceptual models predict that an increase in stress shifts interactions between plants from competitive to facilitative; hence, facilitation is expected to gain in ecological importance with increasing stress. Little is known about how facilitative interactions between plants change with increasing biotic stress, such as that incurred by consumer pressure or herbivory (i.e. disturbance sensu Grime). In grazed ecosystems, the presence of unpalatable plants is reported to protect tree saplings against cattle grazing and enhance tree establishment. In accordance with current conceptual facilitation-stress models, we hypothesised a positive relationship between facilitation and grazing pressure. We tested this hypothesis in a field experiment in which tree saplings of four different species (deciduous Fagus sylvatica, Acer pseudoplatanus and coniferous Abies alba, Picea abies) were planted either inside or outside of the canopy of the spiny nurse shrub Rosa rubiginosa in enclosures differing in grazing pressure (low and high) and in exclosures. During one grazing season we followed the survival of the different tree saplings and the level of browsing on these; we also estimated browsing damage to the nurse shrubs. Shrub damage was highest at the higher grazing pressure. Correspondingly, browsing increased and survival decreased in saplings located inside the canopy of the shrubs at the high grazing pressure compared to the low grazing pressure. Saplings of both deciduous species showed a higher survival than the evergreens, while sapling browsing did not differ between species. The relative facilitation of sapling browsing and sapling survival ¿ i.e. the difference between saplings inside and outside the shrub canopy ¿ decreased at high grazing pressure as the facilitative species became less protective. Interestingly, these findings do not agree with current conceptual facilitation-stress models predicting increasing facilitation with abiotic stress. We used our results to design a conceptual model of facilitation along a biotic environmental gradient. Empirical studies are needed to test the applicability of this model. In conclusion, we suggest that current conceptual facilitation models should at least consider the possibility of decreasing facilitation at high levels of stress
Onderwijsvisitatierapport Bedrijfswetenschappen: Faculteit Bedrijf, Bestuur en Technologie, medeauteur QANU (Quality Assurance Netherlands Universities)
Edelman Bos, J.B.M. ; Dopper, J.G. ; Omta, S.W.F. ; Sluis, H. ; Vandenberghe, R. ; Zwaan, A. - \ 2007
Utrecht : Quality Assurance Netherlands Universities (QANU) - 79
onderwijs - onderwijsbeleid - hoger onderwijs - managementonderwijs - bedrijfswetenschap - leerplan - universiteiten - kwaliteitscontroles - lerarenopleidingen - evaluatie - prestatieniveau - monitoring - nederland - education - educational policy - higher education - management education - management science - curriculum - universities - quality controls - educational courses - evaluation - performance - netherlands
Dit rapport is onderdeel van de kwaliteitsbeoordeling van universitaire bachelor- en master-opleidingen in Nederland. Het doel van het rapport is om een betrouwbaar beeld te geven van de resultaten van de voor beoordeling voorgelegde opleidingen, alsmede een terugkoppeling te geven naar de interne kwaliteitszorg van de betrokken organisaties en als basis te dienen voor de accreditatie van de betrokken opleidingen door de Nederlands-Vlaamse Accreditatie Organisatie (NVAO).
Ecological anachronisms in the recruitment of temperate light-demanding tree species in wooded pastures
Bakker, E.S. ; Olff, H. ; Vandenberghe, C. ; Maeyer, K. de; Smit, R. ; Gleichman, J.M. - \ 2004
Journal of Applied Ecology 41 (2004)3. - ISSN 0021-8901 - p. 571 - 582.
plant spinescence - diversity - dynamics - forest - oak - regeneration - herbivory - availability - facilitation - competition
1. Light-demanding trees and thorny shrubs in temperate plant communities may reflect adaptations to now-extinct large grazers, such as aurochs and tarpans, rendering these adaptations ecological anachronisms. 2. We explored the ecological functions of plant traits of Quercus robur and Prunus spinosa in areas grazed by cattle and horses, the domesticated descendants of aurochs and tarpans. Specifically, we tested the hypothesis that grazing induces a shifting mosaic of grassland, shrub thickets and woodlands through the key process of associational resistance: the protection of palatable young trees by thorny shrubs. 3. An exclosure experiment with transplanted Q. robur seedlings revealed that Q. robur grew best in grassland exclosures and on the edge of thorny shrub thickets, which may be viewed as an optimal balance between sufficient protection from large herbivores and sufficient light availability. 4. A cross-site comparison of four floodplain woodlands in north-western Europe showed that Q. robur can regenerate in the presence of large herbivores through spatial association with P. spinosa. However, we found that expansion of P. spinosa shrubs and Q. robur coincided with periods of low rabbit abundance and not with livestock density. From this, it appears that the process of associational resistance does not work with rabbits. 5. Synthesis and applications. With extensive grazing by large (domesticated) grazers in temperate floodplains, a shifting mosaic of grassland, shrubs and trees may develop that has high conservation value. Palatable, light-demanding Q. robur seedlings can successfully regenerate in spiny P. spinosa shrubs through associational resistance. This process does not offer protection from abundant small herbivores, such as rabbits, that can inhibit the recruitment of shrubs and trees in this mosaic vegetation. In floodplain meadows frequent flooding may be an efficient way to reduce rabbit populations, with dry conditions in summer and wet in winter. When floodplain meadows are combined with adjacent higher grounds, large herbivores can escape the floods through migration.
Process-based modelling of fluvial system response to rapid climate change: 2. application to the River Maas (The Netherlands) during the Last Glacial-Interglacial Transition
Bogaart, P.W. ; Balen, R.T. van; Kasse, C. ; Vandenberghe, J. - \ 2003
Quaternary Science Reviews 22 (2003)20. - ISSN 0277-3791 - p. 2097 - 2110.
holocene transition - lower reach - meuse maas - ice-core - southern netherlands - europe - reconstruction - stratigraphy - landscape - incision
A comprehensive process-based numerical model of catchment hydrology and alluvial channel dynamics is applied to the evolution of the river Maas during the Last Glacial-Interglacial Transition. Palaeo-climatological reconstructions based on a number of climatic and environmental proxies are combined with atmospheric circulation model predictions to yield continuous time series for temperature, precipitation and vegetation cover for the period of 14-9 C-14 kyr BP. These climatic data are used as input for the numerical model. Predictions are made for discharge statistics, hillslope erosion potential, river channel sediment transport, channel pattern and incision potential. These predictions are compared with reconstructed fluvial dynamics of the Maas during this time period. We find that the major fluvial morphodynamical events can be explained by our model as a result of climate change induced affects. However, relatively high precipitation amounts during GS-1 must be applied in order to correctly predict floodplain wide incision during this period. We further show by means of a number of sensitivity analyses that the uncertainty in the adopted climate reconstructions do not have a large impact on modelled morphodynamics. (C) 2003 Elsevier Science Ltd. All rights reserved.
Process-based modelling of fluvial system response to rapid climate change: 1. model formulation and generic applications
Bogaart, P.W. ; Balen, R.T. van; Kasse, C. ; Vandenberghe, J. - \ 2003
Quaternary Science Reviews 22 (2003)20. - ISSN 0277-3791 - p. 2077 - 2095.
north-atlantic - ice core - river - erosion - evolution - maas - similar-to-14.7 - netherlands - landscape - holocene
A comprehensive model strategy is presented which enables the prediction of catchment hydrology and the dynamics of sediment transport within the alluvial river systems draining these catchments. The model is driven by AGCM-based weather predictions, generalised by using a stochastic weather generator, and by palaeo-climate and palaeo-environment reconstructions. The model consists of a lumped hydrological rainfall-runoff model, calibrated against modern daily discharge data and the AGCM control experiment. combined with simple modules for hillslope erosion, river channel geometry, sediment transport and fluvial planform type. We apply the model to a conceptualised climatic cycle, and investigate the response to brief climatic events. Model predictions are discussed and compared to reconstructed river behaviour. (C) 2003 Elsevier Ltd. All rights reserved.
Process based modelling of the response of the River Maas (The Netherlands) to the rapid climate changes of the Last Glacial-Interglacial Transition
Bogaart, P.W. ; Kasse, C. ; Balen, R.T. van; Vandenberghe, J. - \ 2003
Discussion: oil seepage or fossil podzol? An early oligocene oil seepage at the southern rim of the North See Basin, near Leuven (Belgium) by E.D. van Riessen & N. Vandenberghe, Geologie en Mijnbouw 74: 301-312 (1996)
Buurman, P. ; Jongmans, A.G. ; Kasse, C. ; Lagen, B. van - \ 1999
Netherlands journal of geosciences 77 (1999). - ISSN 0016-7746 - p. 93 - 98.
podzolen - fossiele gronden - belgië - altruïsme - holoceen - podzols - fossil soils - belgium - altruism - holocene
Reactie op het artikel van ED van Riessen en N. Vandenberghe in Geologie en mijnbouw (1996), p 301 ev.
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