Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Records 1 - 11 / 11

  • help
  • print

    Print search results

  • export

    Export search results

  • alert
    We will mail you new results for this query: q=Vanhaecke
Check title to add to marked list
Nutrimetabolomics: An Integrative Action for Metabolomic Analyses in Human Nutritional Studies
Ulaszewska, Marynka M. ; Weinert, Christoph H. ; Trimigno, Alessia ; Portmann, Reto ; Andres Lacueva, Cristina ; Badertscher, René ; Brennan, Lorraine ; Brunius, Carl ; Bub, Achim ; Capozzi, Francesco ; Cialiè Rosso, Marta ; Cordero, Chiara E. ; Daniel, Hannelore ; Durand, Stéphanie ; Egert, Bjoern ; Ferrario, Paola G. ; Feskens, Edith J.M. ; Franceschi, Pietro ; Garcia-Aloy, Mar ; Giacomoni, Franck ; Giesbertz, Pieter ; González-Domínguez, Raúl ; Hanhineva, Kati ; Hemeryck, Lieselot Y. ; Kopka, Joachim ; Kulling, Sabine E. ; Llorach, Rafael ; Manach, Claudine ; Mattivi, Fulvio ; Migné, Carole ; Münger, Linda H. ; Ott, Beate ; Picone, Gianfranco ; Pimentel, Grégory ; Pujos-Guillot, Estelle ; Riccadonna, Samantha ; Rist, Manuela J. ; Rombouts, Caroline ; Rubert, Josep ; Skurk, Thomas ; Sri Harsha, Pedapati S.C. ; Meulebroek, Lieven Van; Vanhaecke, Lynn ; Vázquez-Fresno, Rosa ; Wishart, David ; Vergères, Guy - \ 2018
Molecular Nutrition & Food Research 63 (2018)1. - ISSN 1613-4125
GC–MS - LC–MS - metabolomics - NMR - nutrition
The life sciences are currently being transformed by an unprecedented wave of developments in molecular analysis, which include important advances in instrumental analysis as well as biocomputing. In light of the central role played by metabolism in nutrition, metabolomics is rapidly being established as a key analytical tool in human nutritional studies. Consequently, an increasing number of nutritionists integrate metabolomics into their study designs. Within this dynamic landscape, the potential of nutritional metabolomics (nutrimetabolomics) to be translated into a science, which can impact on health policies, still needs to be realized. A key element to reach this goal is the ability of the research community to join, to collectively make the best use of the potential offered by nutritional metabolomics. This article, therefore, provides a methodological description of nutritional metabolomics that reflects on the state-of-the-art techniques used in the laboratories of the Food Biomarker Alliance (funded by the European Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL)) as well as points of reflections to harmonize this field. It is not intended to be exhaustive but rather to present a pragmatic guidance on metabolomic methodologies, providing readers with useful “tips and tricks” along the analytical workflow.
Toward a new european threshold to discriminate illegally administered from naturally occurring thiouracil in livestock
Wauters, Jella ; Vanden Bussche, Julie ; Bizec, Bruno Le ; Kiebooms, J.A.L. ; Dervilly-Pinel, Gaud ; Prevost, Stéphanie ; Wozniak, Barbara ; Sterk, S.S. ; Grønningen, Dag ; Kennedy, D.G. ; Russell, Sandra ; Delahaut, Philippe ; Vanhaecke, Lynn - \ 2015
Journal of Agricultural and Food Chemistry 63 (2015)5. - ISSN 0021-8561 - p. 1339 - 1346.
endogenous - European - LC-MS/MS - thiouracil - threshold - urine

Thiouracil is a thyrostat inhibiting the thyroid function, resulting in fraudulent weight gain if applied in the fattening of livestock. The latter abuse is strictly forbidden and monitored in the European Union. Recently, endogenous sources of thiouracil were identified after frequently monitoring low-level thiouracil positive urine samples and a "recommend concentration" (RC) of 10 ∼g/L was suggested by the EURL to facilitate decision-making. However, the systematic occurrence of urine samples exceeding the RC led to demands for international surveys defining an epidemiologic threshold. Therefore, six European member states (France, Poland, The Netherlands, United Kingdom, Norway, and Belgium) have shared their official thiouracil data (2010-2012) collected from bovines, porcines, and small livestock with 95 and 99% percentiles of 8.1 and 18.2 ∼g/L for bovines (n = 3894); 7.4 and 13.5 ∼g/L for porcines (n = 654); and 7.4 ∼g/L (95% only) for small livestock (n = 85), respectively. Bovine percentiles decreased with the animal age (nonadults had significantly higher levels for bovines), and higher levels were observed in male bovines compared to female bovines.

Fecal microbiota are altered and concentration of volatile fatty acids decreased in dogs with inflammatory bowel disease
Xu, J. ; Verbrugghe, A. ; Lourenço, M. ; Liu, D. ; Daminet, S. ; Eeckhaut, V. ; Immerseel, F. van; Vanhaecke, L. ; Wiele, T. van der; Niu, Y.F. ; Bosch, G. ; Hesta, M. - \ 2014
Incubation of selected fermentable fibres with feline faecal inoculum: correlations between in vitro fermentation characteristics and end products
Rochus, K. ; Bosch, G. ; Vanhaecke, L. ; Velde, H. van de; Depauw, S. ; Xu, J. ; Fievez, V. ; Wiele, T. van der; Hendriks, W.H. ; Janssens, G.P.J. ; Hesta, M. - \ 2013
Archives of Animal Nutrition 67 (2013)5. - ISSN 1745-039X - p. 416 - 431.
gas-production kinetics - chain fatty-acids - dietary fiber - domestic cat - human gut - substrate - feces - food - progression - propionate
This study aimed to evaluate correlations between fermentation characteristics and end products of selected fermentable fibres (three types of fructans, citrus pectin, guar gum), incubated with faecal inocula from donor cats fed two diets, differing in fibre and protein sources and concentrations. Cumulative gas production was measured over 72 h, fermentation end products were analysed at 4, 8, 12, 24, 48 and 72 h post-incubation, and quantification of lactobacilli, bifidobacteria and bacteroides in fermentation liquids were performed at 4 and 48 h of incubation. Partial Pearson correlations, corrected for inoculum, were calculated to assess the interdependency of the fermentation characteristics of the soluble fibre substrates. Butyric and valeric acid concentrations increased with higher fermentation rates, whereas acetic acid declined. Concentrations of butyric acid (highest in fructans) and propionic acid were inversely correlated with protein fermentation end products at several time points, whereas concentrations of acetic acid (highest in citrus pectin) were positively correlated with these products at most time points. Remarkably, a lack of clear relationship between the counts of bacterial groups and their typically associated products after 4 h of incubation was observed. Data from this experiment suggest that differences in fibre fermentation rate in feline faecal inocula coincide with typical changes in the profile of bacterial fermentation products. The observed higher concentrations of propionic and butyric acid as a result of fibre fermentation could possibly have beneficial effects on intestinal health, and may be confounded with a concurrent decrease in the production of putrefactive compounds. In conclusion, supplementing guar gum or fructans to a feline diet might be more advantageous compared with citrus pectin. However, in vivo research is warranted to confirm these conclusions in domestic cats.
Understanding the effect of carbon status on stem diameter variations
Swaef, T. de; Driever, S.M. ; Meulebroek, L. van; Vanhaecke, L. ; Marcelis, L.F.M. ; Steppe, K. - \ 2013
Annals of Botany 111 (2013)1. - ISSN 0305-7364 - p. 31 - 46.
daily trunk shrinkage - fruit-growth - tomato leaves - matter production - transport model - sugar-transport - sap flow - plant - water - phloem
Background Carbon assimilation and leaf-to-fruit sugar transport are, along with plant water status, the driving mechanisms for fruit growth. An integrated comprehension of the plant water and carbon relationships is therefore essential to better understand water and dry matter accumulation. Variations in stem diameter result from an integrated response to plant water and carbon status and are as such a valuable source of information. Methods A mechanistic water flow and storage model was used to relate variations in stem diameter to phloem sugar loading and sugar concentration dynamics in tomato. The simulation results were compared with an independent model, simulating phloem sucrose loading at the leaf level based on photosynthesis and sugar metabolism kinetics and enabled a mechanistic interpretation of the ‘one common assimilate pool’ concept for tomato. Key Results Combining stem diameter variation measurements and mechanistic modelling allowed us to distinguish instantaneous dynamics in the plant water relations and gradual variations in plant carbon status. Additionally, the model combined with stem diameter measurements enabled prediction of dynamic variables which are difficult to measure in a continuous and non-destructive way, such as xylem water potential and phloem hydrostatic potential. Finally, dynamics in phloem sugar loading and sugar concentration were distilled from stem diameter variations. Conclusions Stem diameter variations, when used in mechanistic models, have great potential to continuously monitor and interpret plant water and carbon relations under natural growing conditions
Thyreostatic drugs, stability in bovine and porcine urine
Bussche, J.V. ; Sterk, S.S. ; Brabander, H.F. de; Blokland, M.H. ; Deceuninck, Y. ; Bizec, B. le; Vanhaecke, L. - \ 2012
Analytical and Bioanalytical Chemistry 403 (2012)10. - ISSN 1618-2642 - p. 2973 - 2982.
mass-spectrometry
Thyreostatic drugs, illegally administrated to livestock for fattening purposes, are banned in the European Union since 1981. For monitoring their illegal use, sensitive and specific analytical methods are required. In this context, the knowledge of the stability in a matrix is of primary importance. This study aimed at evaluating the effects of preservation, number of freeze-thaw cycles, and matrix-related variables on the stability of thyreostatic drugs in the urine of livestock. Finally, the developed conservation approach was applied on incurred urine samples, which displayed traces of the thyreostat thiouracil below the recommended concentration of 10 mu g L-1. The stability study confirmed the negative influence of preservation (8 h) at room temperature and at -70 A degrees C, decreases in concentration of more than 78.0% were observed for all thyreostats, except for 1-methyl-2-mercaptoimidazole and 2-mercaptobenzimidazole. Additionally, investigation of matrix-related variables indicated significant impacts of the presence of copper (p = 0.001) and the pH (p = 0.002). Next, an optimised pre-treatment (pH 1 and 0.1 M ethylenediaminetetraacetic acid disodium salt dehydrate) significantly differing from the original conservation approach (p <0.05) was developed, which proved capable of delaying the decrease in concentration and improved the detection in time for both spiked as well as incurred urine samples. In the future, it seems highly advisable to apply the developed pre-treatment on incurred urines upon sampling, before thyreostat analysis. Additionally, it is recommendable to limit preservation of urine samples at room temperature, but also in the freezer prior to thyreostat analysis.
A review of analytical strategies for the detection of endogenous' steroid abuse in food production
Scarth, J.P. ; Kay, J. ; Teale, P. ; Akre, C. ; Bizec, B. le; Brabander, H.F. de; Vanhaecke, L. ; Ginkel, L.A. van; Points, J. - \ 2012
Drug Testing and Analysis 4 (2012). - ISSN 1942-7603 - p. 40 - 49.
tandem mass-spectrometry - performance liquid-chromatography - carbon-isotope analysis - meat-producing animals - gas-chromatography - anabolic-steroids - residue analysis - doping control - veal calves - pattern-recognition
Detection of the abuse of synthetic steroids in food production is nowadays relatively straightforward using modern techniques such as gas or liquid chromatography coupled to mass spectrometry (GC-MS/MS or LC-MS/MS, respectively). However, proving the abuse of endogenous (or naturally occurring) steroids is more difficult. Despite these difficulties, significant progress in this area has recently been made and a number of methods are now available. The aim of the current review was to systematically review the available analytical approaches, which include threshold concentrations, qualitative marker metabolites, intact steroid esters, gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS), longitudinal testing and omics biomarker profiling. The advantages/disadvantages of these methods are considered in detail, but the choice of which to adopt is dictated by a number of practical, political, and economic factors, which vary in different parts of the world. These include the steroid/species combination requiring analysis, the matrix tested, whether samples are collected from live or slaughtered animals, available analytical instrumentation, sample throughput/cost, and the relevant legal/regulatory frameworks. Furthermore, these approaches could be combined in a range of different parallel and/or sequential screening/confirmatory testing streams, with the final choice being determined by the aforementioned considerations. Despite these advances, more work is required to refine the different techniques and to respond to the ever increasing list of compounds classified as endogenous. At this advanced stage, however, it is now more important than ever for scientists and regulators from across the world to communicate and collaborate in order to harmonize and streamline research efforts. (c) 2012 HFL Sport Science (LGC Ltd) and (c) Her Majesty the Queen in Right of Canada.
European Analytical Criteria: Past, Present and Future
Vanhaecke, L. ; Gowik, P. ; Bizec, B. Le; Ginkel, L.A. van; Bichon, E. ; Blokland, M.H. ; Brabander, H.F. de - \ 2011
Journal of AOAC International 94 (2011)2. - ISSN 1060-3271 - p. 360 - 372.
ratio mass-spectrometry - carbon-isotope analysis - performance liquid-chromatography - meat-producing animals - veterinary drugs - urinary steroids - residue analysis - bovine urine - cattle urine - lc-ms
In this paper, the past, present, and (possible) future of the European analytical criteria for residues are described. The elaboration of the revision of Commission Decision 93/256/EC was a long process starting in 1996 and ending with the formation of a European Commission (EC) working group in 1998. This working group took account of developments in scientific and technical knowledge at that time and produced a draft version of the revision within 6 months. The revision, finally published in 2002 (2002/657/EC), includes new ideas on the identification of analytes and the criteria for performance assessment as well as validation procedures. Currently (2009), the evolution in analytical equipment and progress in scientific research, accompanied by recent European regulatory changes, demands an update or revision of the 2002/657/EC.
A new method to determine the energy saving night temperature for vegetative growth of Phalaenopsis
Pollet, B. ; Kromwijk, J.A.M. ; Vanhaecke, L. ; Dambre, P. ; Labeke, M.C. ; Marcelis, L.F.M. ; Steppe, K. - \ 2011
Annals of Applied Biology 158 (2011)3. - ISSN 0003-4746 - p. 331 - 345.
crassulacean acid metabolism - chlorophyll fluorescence parameters - phosphoenolpyruvate carboxylase - ananas-comosus - clusia-minor - photosynthetic efficiency - day/night temperature - cam photosynthesis - co2 concentration - heat tolerance
Knowledge of the energy saving night temperature (i.e. a relatively cool night temperature without affecting photosynthetic activity and physiology) and a better understanding of low night temperature effects on the photosynthetic physiology of Phalaenopsis would improve their production in terms of greenhouse temperature control and energy use. Therefore, Phalaenopsis‘Hercules’ was subjected to day temperatures of 27.5°C and night temperatures of 27.0°C, 24.2°C, 21.2°C, 18.3°C, 15.3°C or 12.3°C in a growth chamber. A new tool for the determination of the energy saving night temperature range was developed based on temperature response curves of leaf net CO2 exchange, chlorophyll fluorescence, organic acid content and carbohydrate concentrations. The newly developed method was validated during a complete vegetative cultivation in a greenhouse environment with eight Phalaenopsis hybrids (i.e. ‘Boston’, ‘Bristol’, ‘Chalk Dust', ‘Fire Fly’, ‘Lennestadt’, ‘Liverpool’, ‘Precious’, ‘Vivaldi’) and day/night temperature set points of 28/28°C, 29/23°C and 29/17°C. Temperature response curves revealed an overall energy saving night temperature range for nocturnal CO2 uptake, carbohydrate metabolism, organic acid accumulation and photosystem II (PSII) photochemistry of 17.1°C to 19.9°C for Phalaenopsis‘Hercules’. At the lower end of this energy saving night temperature range, a high malate-to-citrate ratio switched towards a low ratio and this transition seemed to alleviate effects of night chilling induced photoinhibition. At night temperatures of 24°C or higher, the degradation of starch, glucose and fructose indicated an increased respiratory CO2 production. During the greenhouse validation experiment, the differences between the eight Phalaenopsis hybrids with regard to their response to the warm day/cool night temperature regimes were remarkably large. In general, the day/night temperature of 29/17°C led to a significantly lower biomass accumulation and less leaves which were in addition shorter, narrower and smaller in size as compared to the day/night temperature regimes of 28/28°C and 29/23°C. During week 25 of the cultivation period, plants matured and flower initiation steeply increased for all hybrids and in each day/night temperature regime. Before week 25, early spiking was only sufficiently suppressed in the 29/23°C and 29/17°C temperature regimes for three hybrids (‘Boston’, ‘Bristol’ and ‘Lennestadt’) but not in the other five hybrids. Although a considerable biochemical flexibility was demonstrated for Phalaenopsis‘Hercules’, inhibition of flowering after exposure to a combination of warm days and cool nights appeared to be largely hybrid dependent
Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response
Peters, S.J.A.C. ; Vanhaecke, T. ; Papeleu, P. ; Rogiers, V. ; Haagsman, H.P. ; Norren, K. van - \ 2010
Cell and Tissue Research 340 (2010)3. - ISSN 0302-766X - p. 451 - 457.
adult human hepatocytes - long-term culture - reference distributions - hepatoma-cells - collagen-gel - large cohort - in-vitro - inflammation - albumin - serum
Three different primary rat hepatocyte culture methods were compared for their ability to allow the secretion of fibrinogen and albumin under basal and IL-6- stimulated conditions. These culture methods comprised the co-culture of hepatocytes with rat liver epithelial cells (CCRLEC), a collagen type I sandwich culture (SW) and a conventional primary hepatocyte monolayer culture (ML). Basal albumin secretion was most stable over time in SW. Fibrinogen secretion was induced by IL-6 in all cell culture models. Compared with ML, CC-RLEC showed an almost three-fold higher fibrinogen secretion under both control and IL-6-stimulated conditions. Induction of fibrinogen release by IL-6 was lowest in SW. Albumin secretion was decreased after IL-6 stimulation in both ML and CC-RLEC. Thus, cells growing under the various primary hepatocyte cell culture techniques react differently to IL-6 stimulation with regard to acute-phase protein secretion. CC-RLEC is the preferred method for studying cytokine-mediated induction of acute-phase proteins, because of the pronounced stimulation of fibrinogen secretion upon IL-6 exposure under these conditions.
In vitro evaluation of fiber and protein fermentation substrates in cats
Rochus, K. ; Janssens, G.P.J. ; Bosch, G. ; Hendriks, W.H. ; Vanhaecke, L. ; Hesta, M. - \ 2010
In: The Waltham International Nutritional Sciences Symposium; Pet Nutrition- Art or Science?, Cambridge, UK, 16 - 19 September, 2010. - Cambridge, UK : Waltham Centre for Pet Nutrition - p. 48 - 48.
Check title to add to marked list

Show 20 50 100 records per page

 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.