Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Diversity and linkage disequilibrium analysis wihtin a selected set of cultivated tomatoes
Berloo, R. van; Zhu, A. ; Ursem, R.A. ; Verbakel, H. ; Gort, G. ; Eeuwijk, F.A. van - \ 2008
Theoretical and Applied Genetics 117 (2008). - ISSN 0040-5752 - p. 89 - 101.
fragment-length-polymorphism - lycopersicon-esculentum - genetic diversity - rapd markers - aflp markers - software - accessions - stability - inference - map
Within the Dutch genomics initiative the ¿Centre for Biosystems Genomics¿ (CBSG) a major research effort is directed at the identification and unraveling of processes and mechanisms affecting fruit quality in tomato. The basis of this fruit quality program was a diverse set of 94 cultivated tomato cultivars, representing a wide spectrum of phenotypes for quality related traits. This paper describes a diversity study performed on these cultivars, using information of 882 AFLP markers, of which 304 markers had a known map position. The AFLP markers were scored as much as possible in a co-dominant fashion. We investigated genome distribution and coverage for the mapped markers and conclude that it proved difficult to arrive at a dense and uniformly distributed coverage of the genome with markers. Mapped markers and unmapped markers were used to investigate population structure. A clear substructure was observed which seemed to coincide with a grouping based on fruit size. Finally, we studied amount and decay of linkage disequilibrium (LD) along the chromosomes. LD was observed over considerable (genetic) distances. We discuss the feasibility of marker-trait association studies and conclude that the amount of genetic variation in our set of cultivars is limited, but that there exists scope for association studies
A note on the measurement of genetic diversity within genebank accessions of lettuce (Lactuca sativa L.) using AFLP markers
Jansen, J. ; Verbakel, H. ; Peleman, J. ; Hintum, T.J.L. van - \ 2006
Theoretical and Applied Genetics 112 (2006)3. - ISSN 0040-5752 - p. 554 - 561.
collection - management - tools
This paper discusses a statistical approach for measuring genetic diversity within genebank accessions of a self-fertilising species. This approach is applied to lettuce (Lactuca sativa L.), using AFLP marker data on a set of 1,390 accessions, representing six different lettuce types. Knowledge of the within-accession genetic diversity is important for decisions about the way accessions have to be maintained by genebanks. It is argued that if the within-accession diversity is small, as can be expected for a self-fertilising species like L. sativa, the best approach is to sample as many accessions as possible with only two plants per accession and estimate the within-accession diversity by the proportion of accessions of which the individuals are different
An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum x L. pennellii F2 populations
Haanstra, J.P.W. ; Wye, C. ; Verbakel, H. ; Meijer-Dekens, F. ; Berg, P. van den; Odinot, P. ; Heusden, A.W. van; Tanksley, S. ; Lindhout, P. ; Peleman, J. - \ 1999
Theoretical and Applied Genetics 99 (1999)1. - ISSN 0040-5752 - p. 1 - 2.
The phytopathogenic fungus Cladosporium fulvum is not sensitive to the chitinase and 1,3-ß-glucanase defence proteins of its host, tomato.
Joosten, M.H.A.J. ; Verbakel, H.M. ; Nettekoven, M.E. ; Leeuwen, J. van; Vossen, R.T.M. van der; Wit, P.J.G.M. de - \ 1995
Physiological and Molecular Plant Pathology 46 (1995). - ISSN 0885-5765 - p. 45 - 59.
Structure and genomic organisation of the ipiB and ipiO gene clusters of Phytophthora infestans.
Pieterse, C.M.J. ; West, P. van; Verbakel, H.M. ; Brassé, P.W.H.M. ; Berg-Velthuis, G.C.M. van den; Govers, F. - \ 1994
Gene 138 (1994). - ISSN 0378-1119 - p. 67 - 77.
Increased expression of the calmodulin gene of the late blight fungus Phytophthora infestans during pathogenesis on potato.
Pieterse, C.M.J. ; Verbakel, H.M. ; Hoek Spaans, J. ; Davidse, L.C. ; Govers, F. - \ 1993
Molecular Plant-Microbe Interactions 6 (1993). - ISSN 0894-0282 - p. 164 - 172.
Cloning and expression of DNA encoding a ripening form a polypeptide having sulfhydryl oxidase activity
Maat, J. ; Musters, W. ; Stam, H. ; Schaap, P.J. ; Vondervoort, P.J.J. van de; Visser, J. ; Verbakel, J.M.A. - \ 1993
Octrooinummer: EP0565172, verleend: 1993.
The invention relates to recombinant DNA technology for the production of an enzyme having sulfhydryl oxidase ("SOX") activity. This SOX-enzyme can be used where the oxidation of free sulfhydryl groups (thio compounds) to the corresponding disulfides is desirable. SOX enzyme may be used for treatment of bakery products or for removal of off-flavour from milk or beer.
Cloning and expression of DNA encoding a ripening form of a polypeptide having rhamnogalacturonase activity
Musters, W. ; Stam, H. ; Suykerbuyk, M.E. ; Visser, J. ; Verbakel, J.M. - \ 1993
Octrooinummer: EP0570075, verleend: 1993-11-18.
The invention relates to isolation of an Aspergillus gene encoding rhamnogalacturonase (RG-ase) and the construction of recombinant Aspergillus strains with overexpression of RG-ase. These strains can be used for the commercial production of RG-ase. RG-ase is an important enzyme in processes requiring the degradation and/or modification of pectin or modification of pectin-containing vegetable or plant cell wall material. RG-ase may be used in various applications, including the processing of fruits and vegetables, in the extraction of components from vegetable material or for improving the functionality of pectin or pectin-containing vegetable material, food material or plant cell wall material.
Characterization of two putative pathogenicity genes of the fungal tomato pathogen Cladosporium fulvum.
Ackerveken, A.F.J.M. van den; Kan, J.A.L. van; Joosten, M.H.A.J. ; Muisers, J.M. ; Verbakel, H.M. ; Wit, P.J.G.M. de - \ 1993
Molecular Plant-Microbe Interactions 6 (1993). - ISSN 0894-0282 - p. 210 - 215.
Molecular-genetic studies on the avirulence gene avr9 of Cladosporium fulvum.
Ackerveken, A.F.J.M. van den; Kan, J.A.L. van; Verbakel, H.M. ; Wit, P.J.G.M. de - \ 1991
In: Abstract BRIDGE-Meeting The molecular basis of pathogenicity etc. between Cladosporium fulvum and tomato. Wageningen - p. 4 - 4.
Gene expression in Phytophthora infestans during pathogenesis on potato.
Pieterse, C.M.J. ; Bleker, T. ; Verbakel, H. ; Hoek Spaans, J. ; Govers, F.P.M. - \ 1991
In: Abstract EMBO Workshop Molecular biology of filamentous fungi, Berlin,Germany - p. 36 - 36.
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