Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Differences in infectivity and pathogenicity of two Plantago asiatica mosaic virus isolates in lilies
Tanaka, Masashi ; Verbeek, Martin ; Takehara, Miki ; Pham, Khanh ; Lemmers, Miriam ; Slootweg, Casper ; Arie, Tsutomu ; Komatsu, Ken - \ 2018
European Journal of Plant Pathology (2018). - ISSN 0929-1873
Environmental effects - Infectivity - Necrosis - Ornamental lily - Pathogenicity - Plantago asiatica mosaic virus

Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus in the family Alphaflexiviridae and has been isolated from a variety of host plants. In particular, PlAMV isolates from ornamental lilies (Lilium spp.) cause necrotic symptoms in these plants, which significantly reduces their commercial value. However, it has not been clear whether PlAMV isolates from other host plants differ in their infectivity and/or pathogenicity to ornamental lilies, and whether growth conditions affect infectivity and pathogenicity. In this study, we inoculated an edible lily species (Lilium leichtlinii) and seven varieties of ornamental lilies with two PlAMV isolates, an isolate from ornamental lily (PlAMV-OL) and an isolate from edible lily (PlAMV-Li1). We found that PlAMV-OL showed higher infection rates and exhibited necrotic symptoms more frequently in lilies than PlAMV-Li1. Moreover, we observed higher infection rates of PlAMV-OL in open field than in greenhouse, and higher rates of necrotic symptoms in autumn test than in spring test, suggesting that growth conditions and season affect infectivity and pathogenicity of PlAMV in lilies. Our study would provide important information for estimating the risk of necrotic disease caused by PlAMV, as well as for cultivation management preventing the occurrence of the disease.

Factsheet Betekenis van zorgboerderijen voor verschillende doelgroepen
Hassink, J. ; Bruin, Simone de; Verbeek, Hilde ; Buist, Yvette - \ 2018
Wageningen : Wageningen University & Research - 7 p.
well-being - care - greening - urban areas - health - education - cooperation - social care farms
The G-protein γ subunit of Phytophthora infestans is involved in sporangial development
Hoogen, Johan van den; Verbeek-de Kruif, Natalie ; Govers, Francine - \ 2018
Fungal Genetics and Biology 116 (2018). - ISSN 1087-1845 - p. 73 - 82.
G-protein subunit - GPCR - Oomycetes - Phytophthora - Signaling

The oomycete Phytophthora infestans is a notorious plant pathogen with potato and tomato as its primary hosts. Previous research showed that the heterotrimeric G-protein subunits Gα and Gβ have a role in zoospore motility and virulence, and sporangial development, respectively. Here, we present analyses of the gene encoding a Gγ subunit in P. infestans, Pigpg1. The overall similarity of PiGPG1 with non-oomycete Gγ subunits is low, with only the most conserved amino acids maintained, but similarity with its homologs in other oomycetes is high. Pigpg1 is expressed in all life stages and shows a similar expression profile as the gene encoding the Gβ subunit, Pigpb1. To elucidate its function, transformants were generated in which Pigpg1 is silenced or overexpressed and their phenotypes were analyzed. Pigpg1-silenced lines produce less sporangia, which are malformed. Altogether, the results show that PiGPG1 is crucial for proper sporangia development and zoosporogenesis. PiGPG1 is a functional Gγ, and likely forms a dimer with PiGPB1 that mediates signaling.

Plantenvirussen in het vizier
Stijger, I. ; Verbeek, M. - \ 2018
The Perspective of the Instruments : Mediating Collectivity
Boer, Bas de; Molder, Hedwig Te; Verbeek, Peter Paul - \ 2018
Foundations of Science (2018). - ISSN 1233-1821 - p. 1 - 17.
Actor-Network Theory - Postphenomenology - Scientific instruments - Scientific Perspectivism - Technological mediation - Thing knowledge
Numerous studies in the fields of Science and Technology Studies (STS) and philosophy of technology have repeatedly stressed that scientific practices are collective practices that crucially depend on the presence of scientific technologies. Postphenomenology is one of the movements that aims to draw philosophical conclusions from these observations through an analysis of human–technology interactions in scientific practice. Two other attempts that try to integrate these insights into philosophy of science are Ronald Giere’s Scientific Perspectivism (2006) and Davis Baird’s Thing Knowledge (2004). In this paper, these two approaches will be critically discussed from the perspective of postphenomenology. We will argue that Giere and Baird problematically assume that scientific instruments (a) have a determined function, and (b) that all human members of a scientific collective have immediate access to this function. However, these assumptions also allow them to offer a clear answer to the question how scientists can collectively relate to scientific phenomena. Such an answer is not yet (explicitly) formulated within the postphenomenological perspective. By adding a postphenomenological touch to the semiotic approach in Actor-Network Theory, we offer an account of how different individual human–technology relations are integrated into larger scientific collectives. We do so by showing that scientific instruments not only help constitute scientific phenomena, but also the intersubjectivity within such collectives.
Cerebral tryptophan metabolism and outcome of tuberculous meningitis : An observational cohort study
Laarhoven, Arjan van; Dian, Sofiati ; Aguirre-Gamboa, Raúl ; Avila-Pacheco, Julian ; Ricaño-Ponce, Isis ; Ruesen, Carolien ; Annisa, Jessi ; Koeken, Valerie A.C.M. ; Chaidir, Lidya ; Li, Yang ; Achmad, Tri Hanggono ; Joosten, Leo A.B. ; Notebaart, Richard A. ; Ruslami, Rovina ; Netea, Mihai G. ; Verbeek, Marcel M. ; Alisjahbana, Bachti ; Kumar, Vinod ; Clish, Clary B. ; Ganiem, A.R. ; Crevel, Reinout van - \ 2018
The Lancet Infectious Diseases 18 (2018)5. - ISSN 1473-3099 - p. 526 - 535.
Background: Immunopathology contributes to the high mortality of tuberculous meningitis, but the biological pathways involved are mostly unknown. We aimed to compare cerebrospinal fluid (CSF) and serum metabolomes of patients with tuberculous meningitis with that of controls without tuberculous meningitis, and assess the link between metabolite concentrations and mortality. Methods: In this observational cohort study at the Hasan Sadikin Hospital (Bandung, Indonesia) we measured 425 metabolites using liquid chromatography-mass spectrometry in CSF and serum from 33 HIV-negative Indonesian patients with confirmed or probable tuberculous meningitis and 22 control participants with complete clinical data between March 12, 2009, and Oct 27, 2013. Associations of metabolite concentrations with survival were validated in a second cohort of 101 patients from the same centre. Genome-wide single nucleotide polymorphism typing was used to identify tryptophan quantitative trait loci, which were used for survival analysis in a third cohort of 285 patients. Findings: Concentrations of 250 (70%) of 351 metabolites detected in CSF were higher in patients with tuberculous meningitis than in controls, especially in those who died during follow-up. Only five (1%) of the 390 metobolites detected in serum differed between patients with tuberculous meningitis and controls. CSF tryptophan concentrations showed a pattern different from most other CSF metabolites; concentrations were lower in patients who survived compared with patients who died (9-times) and to controls (31-times). The association of low CSF tryptophan with patient survival was confirmed in the validation cohort (hazard ratio 0·73; 95% CI 0·64-0·83; p<0·0001; per each halving). 11 genetic loci predictive for CSF tryptophan concentrations in tuberculous meningitis were identified (p<0·00001). These quantitative trait loci predicted survival in a third cohort of 285 HIV-negative patients in a prognostic index including age and sex, also after correction for possible confounders (p=0·0083). Interpretation: Cerebral tryptophan metabolism, which is known to affect Mycobacterium tuberculosis growth and CNS inflammation, is important for the outcome of tuberculous meningitis. CSF tryptophan concentrations in tuberculous meningitis are under strong genetic influence, probably contributing to the variable outcomes of tuberculous meningitis. Interventions targeting tryptophan metabolism could improve outcomes of tuberculous meningitis. Funding: Royal Dutch Academy of Arts and Sciences; Netherlands Foundation for Scientific Research; Radboud University; National Academy of Sciences; Ministry of Research, Technology, and Higher Education, Indonesia; European Research Council; and PEER-Health.
Torradovirus, an enigmatic plant virus genus.
Verbeek, Martin - \ 2017
12th European Foundation for Plant Pathology & 10th French Society for Plant Pathology
Verbeek, Martin - \ 2017
The Royal Netherlands Society for Plant Pathology (External organisation)
Verbeek, Martin - \ 2017
European Foundation for Plant Pathology (External organisation)
Verbeek, Martin - \ 2017
Onderzoek naar relatie TVX en bodem
Verbeek, Martin ; Stijger, Ineke - \ 2017

Het is al enkele malen geroepen: TVX is een hele grote uitdaging voor de tulp. Het fonds Innovatie Tulp investeert in onderzoek naar dit lastige virus. Martin Verbeek en Ineke Stijger van Wageningen University & Research richten zich op de rol van de bodem in de virusoverdracht.

Variation in the susceptibility of okra (Abelmoschus esculentus L. Moench) genotypes to okra mosaic virus and Podagrica species under field conditions
Asare-Bediako, Elvis ; Agyarko, Faustina ; Verbeek, M. ; Taah, Kingsley J. ; Asare, Aaron ; Agyei, Frimpong K. ; Sarfo, Justice ; Jojo Eghan, Moses ; Combey, Rofella - \ 2017
Journal of Plant Breeding and Crop Science 9 (2017)6. - ISSN 2006-9758 - p. 79 - 89.
A total of 21 okra (Abelmoschus escuentus L. Moench) genotypes were screened for their reactions against okra mosaic disease (OMD) and flea beetles (Podagrica species) infestations in field trials which were conducted from May to October, 2015 (wet season) and November 2015 to March 2016 (dryseason), in order to identify sources of resistance and or tolerance. The trials were laid out in a randomised complete block design (RCBD) with four replications. Field resistance in the genotypes was assessed at 2, 6 and 10 weeks after planting using a 0 to 5 visual scale based on disease symptoms(where 1 denotes no symptom and 5, very severe symptom). Enzyme linked immunosorbent assay (ELISA) was performed to detect the presence of Okra mosaic virus (OkMV) in the okra genotypes.Populations of the flea beetle (Podagrica spp.), the vector of OkMV, and the associated leaf and fruit damage were also assessed. All the okra genotypes exhibited a varying range of disease symptoms and the flea beetle infestations, and lacked immunity. Genotypes GH2052, GH2063, GH2026, GH3760,GH5302, GH5332, GH5793, GH6105 and UCCC6 exhibited mild symptoms of OMD, and were less susceptible to flea beetle infestation and associated leaf damage during both seasons. Using ELISA, OkMV was detected in all the 21 genotypes. The mean number of fruits per plant and the mean fruit yield (t ha-1) differed significantly (P<0.05) among the okra genotypes. Genotype GH5332 had the highest fruit yield of 11.88 t ha-1 followed by genotype GH6105 (9.34 t ha-1). Percentage fruit damage due to the flea beetle infestation differed significantly among the okra genotypes, ranging between 43.7 and 91.2% and from 47 to 84% in both trials respectively.
Diseases of Lily
Chastagner, G.A. ; Tuyl, J.M. van; Verbeek, M. ; Miller, William ; Westerdahl, Becky B. - \ 2017
In: Handbook of Florists' Crops Diseases, Handbook of Plant Disease Management / McGovern, R.J., Elmer, W.H., Springer International Publishing (Handbook of Plant Disease Management ) - ISBN 9783319323749 - 61 p.
The impact of the viruses' transmission strategies
Verbeek, M. ; Stijger, I. ; Vlugt, R.A.A. van der - \ 2017
Prophyta 2017 (2017). - ISSN 0921-5506
New diagnostic and rapid ‘nextgeneration sequencing’ methods facilitate the
discovery of new plant viruses. However, virus detection or elucidation of the
sequence of its dna/rna is no guarantee for understanding its epidemiology.
In order to develop effective control measures in field crops or in greenhouses,
knowledge of how viruses move from one plant to another is essential.
Grote impact verbod neonicotinoïden
Verbeek, M. ; Vlugt, R.A.A. van der; Raaijmakers, Elma ; Tijink, Frank - \ 2017
Boerderij/Akkerbouw 103 (2017)6. - ISSN 0169-0116 - p. A22 - A24.
Dringend alternatieven nodig voor de beheersing van vergelingsziekte in de suikerbietenteelt. De ziekte kan de opbrengst ernstig schaden.
Wanneer weten we wat we eten?
Candel, Jeroen - \ 2017
Toetsontwikkeling en systeemaanpak PSTVd in Dahlia
Leeuwen, P.J. van; Trompert, J.P.T. ; Verbeek, M. ; Lemmers, M.E.C. ; Meekes, E.T.M. - \ 2017
Lisse : Wageningen University & Research - 26 p.
Ziekteverwekkers plantenvirussen en viroïden vragen continu om aandacht
Stijger, I. ; Verbeek, M. - \ 2017
Onder Glas 14 (2017)1. - p. 58 - 59.
Plantenvirussen zijn zeer kleine ziekteverwekkers. Eigenlijk zijn het kleine pakketjes met genetische informatie, een stukje RNA of DNA met een eiwitmantel eromheen. Viroïden zijn nog kleiner, omdat ze geen eiwitmantel bezitten en alleen uit RNA bestaan. Virussen en viroïden kunnen zich alleen vermenigvuldigen in een cel van hun gastheer en daarbij zetten ze zijn huishouding naar hun hand.
ICTV virus taxonomy profile : Ophioviridae
García, María Laura ; Bó, Elena Dal; Graça, John V. da; Gago-Zachert, Selma ; Hammond, John ; Moreno, Pedro ; Natsuaki, Tomohide ; Pallás, Vicente ; Navarro, Jose A. ; Reyes, Carina A. ; Luna, Gabriel Robles ; Sasaya, Takahide ; Tzanetakis, Ioannis E. ; Vaira, Anna María ; Verbeek, Martin ; Lefkowitz, Elliot J. ; Davison, Andrew J. ; Siddell, Stuart G. ; Simmonds, Peter ; Adams, Michael J. ; Smith, Donald B. ; Orton, Richard J. ; Sanfaçon, Hélène - \ 2017
Journal of General Virology 98 (2017)6. - ISSN 0022-1317 - p. 1161 - 1162.
Blueberry mosaic associated virus - Citrus psorosis virus - ICTV - Lettuce ring necrosis virus - Mirafiori lettuce big-vein virus - Ophioviridae - Taxonomy
The Ophioviridae is a family of filamentous plant viruses, with single-stranded negative, and possibly ambisense, RNA genomes of 11.3-12.5 kb divided into 3-4 segments, each encapsidated separately. Virions are naked filamentous nucleocapsids, forming kinked circles of at least two different contour lengths. The sole genus, Ophiovirus, includes seven species. Four ophioviruses are soil-transmitted and their natural hosts include trees, shrubs, vegetables and bulbous or corm-forming ornamentals, both monocots and dicots. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Ophioviridae, which is available at
Aphid transmission of Lettuce necrotic leaf curl virus, a member of atentative new subgroup within the genus Torradovirus
Verbeek, M. ; Dullemans, A.M. ; Vlugt, R.A.A. van der - \ 2017
Virus Research 241 (2017). - ISSN 0168-1702 - p. 125 - 130.
Lettuce necrotic leaf curl virus (LNLCV) was described as the first non-tomato-infecting member of the genus Torradovirus. Until today, the virus was found only in The Netherlands in two different areas in open field crops of lettuce. In 2015, LNLCV was accepted by the ICTV as a new member of the genus Torradovirus. The tomato-infecting (TI) torradoviruses Tomato torrado virus (ToTV), Tomato marchitez virus (ToMarV) and Tomato chocolàte virus (ToChV) are transmitted by at least three whitefly species in a semi-persistent and stylet-borne manner. As LNLCV was transmitted in open fields in The Netherlands, where whiteflies are present only in low incidence, transmission studies were set up to identify the natural vector of LNLCV. Whitefly species which survive Dutch open field conditions during summer, as well as lettuce colonizing aphid species, were tested for their ability to transmit LNLCV. Lengths of acquisition and inoculation periods were chosen in accordance with the conditions for TI torradoviruses. Transmission experiments involving whiteflies were never successful. Transmission with aphids was only successful in case of the lettuce-currant aphid, Nasonovia ribisnigri. Localization of LNLCV virions in N. ribisnigri with a nested RT-PCR indicated the stylets as possible retention sites. The willow-carrot aphid Cavariella aegopodii did not transmit LNLCV in our transmission experiment but the virus could be detected in the stylets of this aphid, leaving C. aegopodii as a possible vector for LNLCV.
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