Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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A new method for analysing socio-ecological patterns of vulnerability
Kok, M. ; Lüdeke, M. ; Lucas, P. ; Sterzel, T. ; Walther, C. ; Janssen, P. ; Sietz, D. ; Soysa, I. de - \ 2016
Regional Environmental Change 16 (2016)1. - ISSN 1436-3798 - p. 229 - 243.
This paper presents a method for the analysis of socio-ecological patterns of vulnerability of people being at risk of losing their livelihoods as a consequence of global environmental change. This method fills a gap in methodologies for vulnerability analysis by providing generalizations of the factors that shape vulnerability in specific socio-ecological systems and showing their spatial occurrence. The proposed method consists of four steps that include both quantitative and qualitative analyses. To start, the socio-ecological system exposed to global environmental changes that will be studied needs to be determined. This could, for example, be farmers in drylands, urban populations in coastal areas and forest-dependent people in the tropics. Next, the core dimensions that shape vulnerability in the socio-ecological system of interest need to be defined. Subsequently, a set of spatially explicit indicators that reflect these core dimensions is selected. Cluster analysis is used for grouping the indicator data. The clusters found, referred to as vulnerability profiles, describe different typical groupings of conditions and processes that create vulnerability in the socio-ecological system under study, and their spatial distribution is provided. Interpretation and verification of these profiles is the last step in the analysis. We illustrate the application of this method by analysing the patterns of vulnerability of (smallholder) farmers in drylands. We identify eight distinct vulnerability profiles in drylands that together provide a global overview of different processes taking place and sub-national detail of their distribution. By overlaying the spatial distribution of these profiles with specific outcome indicators such as conflict occurrence or migration, the method can also be used to understand these phenomena better. Analysis of vulnerability profiles will in a next step be used as a basis for identifying responses to reduce vulnerability, for example, to facilitate the transfer of best practices to reduce vulnerability between different places.
Farmers' vulnerability in African drylands, a quantitative and spatially-explicit typology based on clustering
Sietz, D. ; Ordonez, J.C. ; Kok, M. ; Janssen, P. ; Hilderink, H. ; Lüdeke, M. ; Walther, C. ; Sterzel, T. ; Dijk, H. van - \ 2014
Typologies of socio-ecological conditions; identifying relevant and valid patterns to support resilience building
Sietz, D. ; Lüdeke, M. ; Walther, C. ; Kok, M. ; Janssen, P. - \ 2014
One stop shop: backbones trees for important phytopathogenic genera: I (2014)
Hyde, K.D. ; Nilsson, R.H. ; Alias, S.A. ; Ariyawansa, H.A. ; Blair, J.E. ; Cai, L. ; Cock, A.W.A.M. de; Dissanayake, A.J. ; Glockling, S.L. ; Goonasekara, I.D. ; Gorczak, M. ; Hahn, M. ; Jayawardena, R.S. ; Kan, J.A.L. van; Laurence, M.H. ; Lévesque, C.A. ; Li, X. ; Liu, J.K. ; Maharachchikumbura, S.S.N. ; Manamgoda, D.S. ; Martin, F.N. ; McKenzie, E.H.C. ; McTaggart, A.R. ; Mortimer, P.E. ; Nair, P.V.R. ; Pawlowska, J. ; Rintoul, T.L. ; Shivas, R.G. ; Spies, C.F.J. ; Summerell, B.A. ; Taylor, P.W.J. ; Terhem, R.B. ; Udayanga, D. ; Vaghefi, N. ; Walther, G. ; Wilk, M. ; Wrzosek, M. ; Xu, J.C. ; Yan, J.Y. ; Zhou, N. - \ 2014
Fungal Diversity 67 (2014). - ISSN 1560-2745 - p. 21 - 125.
internal transcribed spacer - ribosomal dna-sequences - vegetative compatibility groups - plant-pathogenic fungi - citrus black spot - spored graminicolous colletotrichum - sporisorium-macalpinomyces complex - fragment-length-polymorphisms - botrytis-cinerea popu
Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry. They are also part of the natural ecosystem and may play a role in regulating plant numbers/density. Morphological identification and analysis of plant pathogenic fungi, while important, is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi. Molecular (DNA sequence) data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies, although hampered in part by non-standard laboratory practices and analytical methods. To facilitate current and future research, this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota, Basidiomycota, Mucormycotina (Fungi), and Oomycota, using recent molecular data, up-to-date names, and the latest taxonomic insights. Lineage-specific laboratory protocols together with advice on their application, as well as general observations, are also provided. We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge. Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort. Bipolaris, Botryosphaeriaceae, Botryosphaeria, Botrytis, Choanephora, Colletotrichum, Curvularia, Diaporthe, Diplodia, Dothiorella, Fusarium, Gilbertella, Lasiodiplodia, Mucor, Neofusicoccum, Pestalotiopsis, Phyllosticta, Phytophthora, Puccinia, Pyrenophora, Pythium, Rhizopus, Stagonosporopsis, Ustilago and Verticillium are dealt with in this paper.
Armed conflict distribution in global drylands through the lens of a typology of socio-ecological vulnerability
Sterzel, T. ; Lüdeke, M. ; Kok, M. ; Soysa, I. De; Walther, C. ; Sietz, D. ; Lucas, P. ; Janssen, P. - \ 2014
Regional Environmental Change 14 (2014)4. - ISSN 1436-3798 - p. 1419 - 1435.
climate-change - civil-war - violent conflict - african sahel - dataset - desertification - institutions - patterns - risk
Motivated by an inconclusive debate over implications of resource scarcity for violent conflict, and common reliance on national data and linear models, we investigate the relationship between socio-ecological vulnerability and armed conflict in global drylands on a subnational level. Our study emanates from a global typology of smallholder farmers’ vulnerability to environmental and socioeconomic stresses in drylands. This typology is composed of eight typical value combinations of variables indicating environmental scarcities, resource overuse, and poverty-related factors in a widely subnational spatial resolution. We investigate the relationships between the spatial distribution of these combinations, or vulnerability profiles, and geocoded armed conflicts, and find that conflicts are heterogeneously distributed according to these profiles. Four profiles distributed across low- and middle-income countries comprise all drylands conflicts. Comparing models for conflict incidence using logit regression and ROC (Receiver Operator Characteristic) analysis based on (1) the set of all seven indicators as independent variables and (2) a single, only vulnerability profile- based variable proves that the non-linear typology-based variable is the better explanans for conflict incidence. Inspection of the profiles’ value combinations makes this understandable: A systematic explanation of conflict incidence and absence across all degrees of natural resource endowments is only reached through varying importance of poverty and resource overuse depending on the level of endowment. These are non-linear interactions between the explaining variables. Conflict does not generally increase with resource scarcity or overuse. Comparison with conflict case studies showed both good agreement with our results and promise in expanding the set of indicators. Based on our findings and supporting literature we argue that part of the debate over implications of resource scarcity for violent conflict in drylands may be resolved by acknowledging and accounting for non-linear processes.
Observation and monitoring needs to support ecosystem-based management - preparing to serve the current of data coming upon us
Boois, I.J. de; Sagen, H. ; Walther, Y. - \ 2013
Reykjavik : ICES
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi
Schoch, C.L. ; Seifert, K.A. ; Huhndorf, S. ; Robert, V. ; Spouge, J.L. ; Levesque, C.A. ; Chen, W. ; Crous, P.W. ; Boekhout, T. ; Damm, U. ; Hoog, G.S. de; Eberhardt, U. ; Groenewald, J.Z. ; Groenewald, M. ; Hagen, F. ; Houbraken, J. ; Quaedvlieg, W. ; Stielow, B. ; Vu, T.D. ; Walther, G. - \ 2012
Proceedings of the National Academy of Sciences of the United States of America 109 (2012)16. - ISSN 0027-8424 - p. 6241 - 6246.
arbuscular mycorrhizal fungi - phylogenetic-relationships - basidiomycetous yeasts - intragenomic variation - ectomycorrhizal fungi - species recognition - sequence-analysis - rpb1 sequences - rdna - subunit
Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as the primary fungal barcode marker to the Consortium for the Barcode of Life, with the possibility that supplementary barcodes may be developed for particular narrowly circumscribed taxonomic groups.
Post-genomic characterization of metabolic pathways in Sulfolobus solfataricus
Walther, J. - \ 2012
University. Promotor(en): John van der Oost; Willem de Vos. - S.l. : s.n. - ISBN 9789461732033 - 162
sulfolobus solfataricus - biochemische omzettingen - koolstofpathways - transcriptomica - bioreactoren - biochemical pathways - carbon pathways - transcriptomics - bioreactors

The physiological functions and mode of actions of different biomolecules are of continuous interest and a prerequisite to fully understand and appreciate the potential of Archaea and their molecules. We chose to study Sulfolobus solfataricus for its stable (heat-resistant) enzymes and specific metabolic potential, the ease of cultivation of this organism, and the relative large amount of knowledge about this heat-loving acidophilic organism. We selected a systems approach to study the behaviour of this organism trying to make steps forward into the unknown, whenever possible trying to link exploration to exploitation. The cultivation of S.solfataricus is an essential element in all systems approaches that link genotype to phenotype. Hence, specific attention is given to the advanced culturing systems for this extremophile that have been used in all experimental studies described here (Chapters 3-6).

Systems analysis includes the integration of all available omics data and is increasingly used in the analysis of Archaea (Chapters 3 and 4). However, most attention has been given to archaeal transcriptome analysis and hence the most important literature on heat-loving Archaea is summarized (Chapter 2).

In the experimental chapters (Chapters 3-6) various systems approaches are applied to gain understanding of metabolic pathways in Sulfolobus. Chapter 3 describes the study of the central carbon pathways, consisting of the (non-) phosphorilated Entner-Douderoff (ED) pathway and the citric acid cycle. Different functional genomic approaches were applied on the model organism Sulfolobus solfataricus to study the response of growth on different carbon sources, D-Glucose vs. Tryptone and Yeast Extract. The complete transcriptome was studied using PCR-based microarrays. In addition the proteome was studied using 2D-electrophoresis map in combination with 13N- labelling technique to determine protein fluctuations. Despite the large difference in medium, very few significant differences on protein or RNA level were observed for the two conditions. Therefore regulation of these pathways does in all probability not occur through changes in protein abundance but presumably rather by direct changes in enzyme activity. This is unlike two thermophilic Euryarchaea: Thermococcus kodaaraensis (Kanai, Akerboom et al. 2007)and Pyrococcus furiosus (Schut, Brehm et al. 2003)where extensive regulation of glycolytic genes was observed in a similar situation.

Chapter 4describes the study of the degradation of D-arabinose through a similar approach as was described in chapter 3. S. solfataricus was grown on either D-arabinose or D-glucose and a comprehensive transcriptome and proteome study was carried out. The result of these studies was not only elucidation of the D-arabinose degradation route, but also a general prokaryotic pentose, hexaric acids and hydroxyproline degradation route, which supports the theory of metabolic pathway genesis by enzyme recruitment. Also this study predicted a cis-regulatory element to induce the arabinose degrading pathway when needed. The enzymes involved in the proposed pathway were cloned, expressed and their function was biochemically measured. This showed that using these enzymes, D-arabinose can be degraded to 2-oxogluterate, one of the metabolites that are part of the citric acid cycle.

Chapter 5reports on the effects of different oxygen concentrations on the behaviour of Sulfolobus solfataricus. The oxygen amount can be controlled relatively easily in a bioreactor, which is crucial for rapid and reproducible growth. Based on growth experiments in microcosms, different types of behaviour could be seen. At 35% (v/v gas phase) the cultures did not grow, indicating that S. solfa-taricus experiences a lethal dose of oxygen. At 26-32% growth was impaired, suggesting a moderate toxicity compared to the reference (21%). In the ranges 16-24% of oxygen, standard growth was observed, suggesting that S. solfataricus is comfortable in these oxygen ranges. For the lower amounts of oxygen (1.5-15%), the growth was comparable to the reference, but the respiratoryefficiency was increased. To get some more insight into this behaviour, we looked at the transcriptome. It showed differential expression of several genes, including genes encoding terminal oxidases, indicating that the organism adapts to lower oxygen concentrations by adapting its respiratory machinery.

Chapter 6 describes the zeaxanthin pathway in the Sulfolobus species. Zeaxanthin is a colorant and of vital importance for the function of the human eye. In this chapter the genes responsible for zeaxanthin production are presented. For this, DNA microarrays, bioinformatics as well as molecular genetics techniques were used. A crtx-like gene is operational in most of the known Sulfolobus species that is able to attach sugar-like molecules to zeaxanthin, which improves its solubility in water, which is very important in many food uses. We have cloned this crtx-like gene of S. solfataricus, S. shibatae, and S. acidocaldarius in a zeaxanthin overproducing E. coli strain. It has been demonstrated that the gene products of S. shibatae and S. acidocaldarius were responsible for attaching sugar-like molecules to zeaxanthin. The ctrx-like gene of S. solfataricus was not operating in E. coli. This is probably due to the fact that the gene is truncated. This chapter has further improved the understanding of archaeal carotenoid pathways and it has shown that the Sulfolobus species are able to modify zeaxanthin, although each species produces different zeaxanthin modifications.

Categorisation of typical vulnerability patterns in global drylands
Sietz, D. ; Lûdeke, M.K.B. ; Walther, C. - \ 2011
Global environmental change : human and policy dimensions 21 (2011)2. - ISSN 0959-3780 - p. 431 - 440.
water-use - rangeland degradation - multiple stressors - land degradation - rural poverty - south-africa - livelihoods - desertification - sustainability - mexico
Drylands display specific vulnerability-creating mechanisms which threaten ecosystems and human well-being. The upscaling of successful interventions to reduce vulnerability arises as an important, but challenging aim, since drylands are not homogenous. To support this aim, we present the first attempt to categorise dryland vulnerability at a global scale and sub-national resolution. The categorisation yields typical patterns of dryland vulnerability and their policy implications according to similarities among the socio-ecological systems. Based on a compilation of prevalent vulnerability-creating mechanisms, we quantitatively indicate the most important dimensions including poverty, water stress, soil degradation, natural agro-constraints and isolation. A cluster analysis reveals a set of seven typical vulnerability patterns showing distinct indicator combinations. These results are validated by case studies reflecting the cluster-specific mechanisms and their spatial distribution. Based on these patterns, we deduce thematic and spatial entry points for reducing dryland vulnerability. Our findings could contribute new insights into allocating the limited funds available for dryland development and support related monitoring efforts based on the manageable number of key indicators.
Hot Transcriptomics
Walther, J. ; Sierocinski, P. ; Oost, J. van der - \ 2010
Archaea : an international microbiological journal 2010 (2010). - ISSN 1472-3646 - 14 p.
archaeon sulfolobus-solfataricus - dna microarray analysis - turreted icosahedral virus - central carbohydrate-metabolism - heat-shock response - hyperthermophilic archaeon - pyrococcus-furiosus - chromosome-replication - thermococcus-kodakaraensis - methanococcus
DNA microarray technology allows for a quick and easy comparison of complete transcriptomes, resulting in improved molecular insight in fluctuations of gene expression. After emergence of the microarray technology about a decade ago, the technique has now matured and has become routine in many molecular biology laboratories. Numerous studies have been performed that have provided global transcription patterns of many organisms under a wide range of conditions. Initially, implementation of this high-throughput technology has lead to high expectations for ground breaking discoveries. Here an evaluation is performed of the insight that transcriptome analysis has brought about in the field of hyperthermophilic archaea. The examples that will be discussed have been selected on the basis of their impact, in terms of either biological insight or technological progress.
From closed-loop to sustainable suplly chains: the WEEE case
Quariguasi Frota Neto, J. ; Walther, G. ; Bloemhof, J.M. ; Nunen, J.A.E.E. van; Spengler, T. - \ 2010
International Journal of Production Research 48 (2010)15. - ISSN 0020-7543 - p. 4463 - 4481.
product recovery - decision-making - green - approximation - management - systems - curves - issues - set - aid
The primary objective of closed-loop supply chains (CLSC) is to improve the maximum economic benefit from end-of-use products. Nevertheless, the literature within this stream of research advocates that closing the loop also helps to mitigate the undesirable environmental footprint of supply chains. Therefore, closed-loop supply chains are assumed to be sustainable supply chains almost by definition. In this paper we analyse if and when this assumption holds. We illustrate our findings based on the Electric and Electronic Equipment (EEE) supply chain. For all phases of the supply chain, i.e. manufacturing, usage, transportation and end-of-life activities, we assess the magnitude of the environmental impacts, based on a single environmental metric, namely the Cumulative Energy Demand (CED). Given the environmental hot-spots in the Electric and Electronic Equipment supply chain, we propose useful extensions for existing CLSC optimisation models to ensure that closed-loop supply chains are at the same time sustainable supply chains
Multicompartment Nanoparticles Formed by a Heparin-Mimicking Block Terpolymer in Aqueous Solutions
Uchman, M. ; Stepanek, M. ; Prochazka, K. ; Mountrichas, G. ; Pispas, S. ; Voets, I.K. ; Walther, A. - \ 2009
Macromolecules 42 (2009)15. - ISSN 0024-9297 - p. 5605 - 5613.
atomic-force microscopy - abc triblock copolymer - onion-type micelles - fluorescence correlation spectroscopy - diblock copolymers - selective solvents - light-scattering - poly(2-vinylpyridine)-block-poly(ethylene oxide) - wormlike micelles - star terpolymers
A new amphiphilic block. terpolymer poly((sulfamate-carboxylate)isoprene)-block-polystyrene-block-poly(ethyl ene oxide), PISC230-PS52-PEO151, with a narrow molecular weight distribution (PDI = 1.05), was synthesized via the post. polymerization reaction of the anionically prepared precursor block terpolymer polyisoprene-block-polystyrene-block--poly(ethylene oxide) with chlorosulfonyl isocyanate. The formation and Structure of self-assemblies of the polyelectrolyte block terpolymer in dilute aqueous Solutions were studied by static and dynamic light scattering, atomic force and cryogenic transmission electron microscopy, fluorometry, and H-1 NMR spectroscopy. In acidic solutions, the terpolymers self-assemble into kinetically trapped multicompartment micelles, with the core consisting of discrete PS and PISC domains and PEO in the shell. If the solution pH is adjusted to the alkaline region, the multicompartment micelles undergo an irreversible transition to regular micelles, with a PS core and a mixed shell formed by PEO and PISC blocks.
A Methodology for Assessing Eco-Efficiency in Logistics Networks
Quariguasi Frota Neto, J. ; Walther, G. ; Bloemhof, J.M. ; Nunen, J.A.E.E. van; Spengler, T. - \ 2009
European Journal of Operational Research 193 (2009)3. - ISSN 0377-2217 - p. 670 - 682.
data envelopment analysis - approximation - management - curves - design
Recent literature on sustainable logistics networks points to two important questions: (i) How to spot the preferred solution(s) balancing environmental and business concerns? (ii) How to improve the understanding of the trade-offs between these two dimensions? We posit that a visual exploration of the efficient frontier and trade-offs between profitability and environmental impacts are particularly suitable to answer these two questions. The visual representation of the efficient frontier, however, presents two challenges. The first is to obtain a good approximation for such frontier without enumerating all extreme efficient solutions. The second is to obtain a good visual representation of the efficient frontier. We propose a two-phased heuristic to handle these two problems. The algorithm is designed for the multi-objective linear problem with three objectives: minimize costs, cumulative energy demand and waste in a reverse logistics network. We illustrate our approach by designing a complex recycling logistics network in Germany
Effect of O2 concentrations on Sulfolobus solfataricus P2
Simon, G. ; Walther, J. ; Zabeti, N. ; Combet-Blanc, Y. ; Auria, R. ; Oost, J. van der; Casalot, L. - \ 2009
FEMS Microbiology Letters 299 (2009)2. - ISSN 0378-1097 - p. 255 - 260.
superoxide-dismutase - thermus-thermophilus - cytochrome ba(3) - oxidase - oxygen - archaeon - stress - identification - metabolism - enzyme
Sulfolobus solfataricus P2 was grown aerobically at various O(2) concentrations. Based on growth parameters in microcosms, four types of behavior could be distinguished. At 35% O(2) (v/v; gas phase), the cultures did not grow, indicating a lethal dose of oxygen. For 26-32% O(2), the growth was significantly affected compared with the reference (21%), suggesting a moderate toxicity by O(2). For 16-24% O(2), standard growth was observed. For 1.5-15% O(2), growth was comparable with the reference, but the yield on O(2) indicated a more efficient use of oxygen. These results indicate that S. solfataricus P2 grows optimally in the range of 1.5-24% O(2), most likely by adjusting its energy-transducing machinery. To gain some insight into control of the respiratory system, transcriptomes of the strain cultivated at different O(2) concentrations, corresponding to each behavior (1.5%, 21% and 26%), were compared using a DNA microarray approach. It showed differential expression of several genes encoding terminal oxidases, indicating an adaptation of the strain's respiratory system in response to fluctuating oxygen concentrations
Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus
Ortmann, A.C. ; Walther, J. ; Brumfield, S.K. ; McInnerney, K. ; Brouns, S.J.J. ; Werken, H.J.G. van de; Bothner, B. ; Douglas, T. ; Oost, J. van der; Young, M.J. - \ 2008
Journal of Virology 82 (2008)10. - ISSN 0022-538X - p. 4874 - 4883.
gene-expression - electron microscopy - particle ssv1 - dna - protein - genome - host - replication - microarrays - chromosome
Microarray analysis of infection by Sulfolobus turreted icosahedral virus (STIV) revealed insights into the timing and extent of virus transcription, as well as differential regulation of host genes. Using a microarray containing genes from both the host and the virus, the infection cycle of STIV was studied. Following infection of Sulfolobus solfataricus strain 2-2-12 with STIV, transcription of virus genes was first detected at 8 h postinfection (p.i.), with a peak at 24 h p.i. Lysis of cells was first detected at 32 h p.i. There was little temporal control of the transcription of virus genes, although the three open reading frames on the noncoding strand were transcribed later in the infection process. During the infection, 177 host genes were determined to be differentially expressed, with 124 genes up-regulated and 53 genes down-regulated. The up-regulated genes were dominated by genes associated with DNA replication and repair and those of unknown function, while the down-regulated genes, mostly detected at 32 h p.i., were associated with energy production and metabolism. Examination of infected cells by transmission electron microscopy revealed alterations in cell ultrastructure consistent with the microarray analysis. The observed patterns of transcription suggest that up-regulated genes are likely used by the virus to reprogram the cell for virus replication, while the down-regulated genes reflect the imminent lysis of the cells.
Wireless Access to Sensor Populations: Potential applications for herd health control , eldery care and road transport
Lokhorst, C. ; Eijk, O.N.M. van; Frumento, E. ; Glisenti, Fulvio ; Goense, D. ; Hogewerf, P.H. ; Ipema, A.H. ; Mazzu, Marco ; Mol, R.M. de; Savio, Walther ; Wells, Hannah - \ 2008
In: Proceedings 3rd ECPLF conference 3-6 june 2007, Skiathos, Greece. - - p. 153 - 160.
WASP D6.2 Specified application requirements and prototype selection (with annex I_IV)
Lokhorst, C. ; Mol, R.M. de; Wells, Hannah ; Mazzu, Marco ; Frumento, F. ; Corongiu, A. ; Savio, Walther ; Ipema, A.H. ; Sorniotti, A. ; Gomez, L.D. - \ 2007
Brussel : Europese Unie - 45
wegen - verkeer - ouderen - volksgezondheid - rundvee - diergezondheid - draadloze sensornetwerken - zorg - roads - traffic - elderly - public health - cattle - animal health - wireless sensor networks - care
Assessing the societal aspects of wireless sensor networks
Mol, R.M. de; Mazzu, Marco ; Savio, Walther ; Wells, Hannah ; Glisenti, Fulvio ; Lokhorst, Kees - \ 2007
WASP Scenario choice: Deliverable D6.1, EU KP6 IST-034963
Mol, R.M. de; Eijk, O.N.M. van; Goense, D. ; Hogewerf, P.H. ; Ipema, A.H. ; Lokhorst, C. ; Frumento, E. ; Mazzu, Marco ; Wells, Hannah ; Savio, Walther ; Wrona, K. - \ 2007
Brussel : Information Science Technologies - 56
wegen - verkeer - ouderen - volksgezondheid - rundvee - diergezondheid - draadloze sensornetwerken - roads - traffic - elderly - public health - cattle - animal health - wireless sensor networks
The hyperthermophilic archaeon Sulfolobus : from exploration to exploitation
Brouns, S.J.J. ; Ettema, T.J.G. ; Stedman, K.M. ; Walther, J. ; Smidt, H. ; Snijders, A.P.L. ; Young, M. ; Bernander, R. ; Wright, P.C. ; Siebers, B. ; Oost, J. van der - \ 2006
In: Geothermal Biology and Geochemistry in Yellowstone National Park / Inskeep, W.P., McDermott., T.R., Bozeman : Montana State University Publications - ISBN 0963511416 - p. 261 - 276.
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