2026-06-09T20:22:51+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/357401 2026-03-31

urn:nbn:nl:ui:32-357401 2026-03-31 urn:nbn:nl:ui:32-357401/mods Identification of plant proteins in adulterated skimmed milk powder by high-performance liquid chromatography-mass spectrometry Luykx D.M.A.M. 172274338 0000000387573647 aut Cordewener J.H.G. 072809353 000000039570466X aut Ferranti P. aut Frankhuizen R. 073237485 000000039410729X aut Bremer M.G.E.G. 296113468 0000000391057151 aut Hooijerink H. aut America A.H.P. 125023022 0000-0002-0167-1194 0000000390072042 aut text info:eu-repo/semantics/article 2007 10.1016/j.chroma.2007.07.017 2-s2.0-34548040972 000249471900024 en The EU subsidises the use of skimmed-milk powder (SMP) in compound feeding stuffs. There are indications of falsified SMP content due to the addition of plant proteins. These proteins are not allowed in SMP and cannot be identified by the official reference method. Since soy and pea proteins are most likely to be added to SMP, manufactured SMP containing 1 and 5% of these plant proteins was used to develop a sensitive protein identification method based on mass spectrometry (MS). The method included a pre-fractionation step to enrich for plant proteins by using a borate buffer. A very fast perfusion liquid chromatography method including sensitive and selective intrinsic fluorescence detection was developed for monitoring and quantifying the efficiency of the pre-fractionation and screening for plant proteins. After tryptic digestion of the enriched fraction from manufactured adulterated SMP, numerous peptides originating from the major seed proteins of soy (glycinin, ß-conglycin) and pea (legumin, vicilin) could be identified by MS/MS analysis on a quadrupole time-of-flight MS instrument. RIKILT - Business Unit Safety & Health PRI Bioscience BU Microbiological & Chemical Food Analysis biomolecules capillary-electrophoresis cheese dairy-products electrospray-ionization fast atom bombardment pea perfusion chromatography reversed-phase soybean proteins RIKILT - Business Unit Veiligheid & Gezondheid PRI Bioscience BU Microbiologische & Chemische Voedselanalyse Journal of Chromatography. A, Including electrophoresis and other separation methods 1164 1-2 189 197 00219673 urn:nbn:nl:ui:32-357401/obj 2026-03-31 http://purl.org/eprint/accessRights/RestrictedAccess