2026-06-09T15:58:59+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/360573 2026-03-31

urn:nbn:nl:ui:32-360573 2026-03-31 urn:nbn:nl:ui:32-360573/mods Comprehensive two-dimensional liquid chromatography with ultraviolet, evaporative light scattering and mass spectrometric detection of triacylglycerols in corn oil van der Klift E.J.C. 314603069 0000000394508472 aut Vivó-Truyols G. aut Claassen F.W. 31462158X 0000000390446219 aut van Holthoon F.L. 26627773X 0000000394124241 aut van Beek T.A. 070558221 0000-0002-9843-7096 0000000083822771 aut text info:eu-repo/semantics/article 2008 10.1016/j.chroma.2007.11.039 2-s2.0-37549023071 000252477400006 en An improved comprehensive two-dimensional (LC × LC) HPLC system for the analysis of triacylglycerols was developed. In the first-dimension, a Ag(I)-coated cation exchanger (250 mm × 2.1 mm, 5 ¿m) was employed with a gradient from 100% MeOH to 6% MeCN in MeOH at 20 ¿L/min. Using a 10-way valve with two switching loops, 1 min sections of the first-dimension were introduced in the second-dimension consisting of a 30 mm × 4.6 mm C18 (1.8 ¿m) column with an isocratic mobile phase of methanol¿methyl tert-butyl ether (70:30) at 3.0 mL/min. As the second-dimension solvent was stronger than the first-dimension solvent, focusing in the second-dimension took place, leading to better separations than in previously reported analyses in which hexane was the main constituent of the first-dimension eluent. Compounds differing by 2 in their partition number were baseline separated in the second-dimension. Detection took place by UV at 210 nm, evaporative light scattering and (+)-atmospheric pressure chemical ionisation-MS with the latter giving the best results. Corn oil was investigated and 44 compounds could be detected: 34 triacylglycerols (TAGs), 8 oxygenated TAGs, and 2 TAGs containing a trans double bond. Data manipulation allowed the construction of contour plots and the automated calculation of the first- and second-dimension retention times and peak areas. Quantitative results are compared with a fatty acid methyl ester analysis, and with literature data. VLAG Organic Chemistry argentation chromatography column fats gas-chromatography lipids matrix performance plant oils reversed-phase separation VLAG Organische Chemie Journal of Chromatography. A, Including electrophoresis and other separation methods 1178 1-2 43 55 00219673 urn:nbn:nl:ui:32-360573/obj 2026-03-31 http://purl.org/eprint/accessRights/RestrictedAccess