2026-06-09T16:52:59+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/379988 2026-03-31

urn:nbn:nl:ui:32-379988 2026-03-31 urn:nbn:nl:ui:32-379988/mods Untargeted LC-Q-TOF mass spectrometry method for the detection of adulterations in skimmed-milk powder Cordewener J.H.G. 072809353 000000039570466X aut Luykx D.M.A.M. 172274338 0000000387573647 aut Frankhuizen R. 073237485 000000039410729X aut Bremer M.G.E.G. 296113468 0000000391057151 aut Hooijerink H. aut America A.H.P. 125023022 0000-0002-0167-1194 0000000390072042 aut text info:eu-repo/semantics/article 2009 10.1002/jssc.200800568 2-s2.0-67649710064 000265793700013 en A nontargeted protein identification method was developed to screen for adulterations in skimmed-milk powder (SMP). There are indications of falsified SMP content due to the addition of plant proteins. To demonstrate the reliability and accuracy of the developed comparative LC-MS method using a quadrupole TOF MS instrument, adulterated SMP samples were prepared by the addition of protein isolates of soy and pea to skimmed-milk before pasteurisation and evaporation. The comparative LC-MS approach enabled unequivocal discrimination of those SMP samples containing soy and pea protein from nonadulterated SMP. To identify the source of (plant) proteins present in the adulterated SMP, data-dependent LC-MS/MS was used in combination with an include list of differential peptides. Numerous peptides originating from the major seed proteins of soy (glycinin, beta-conglycin) and pea (legumin, vicilin) could be identified in this way RIKILT - Business Unit Safety & Health PRI Bioscience BU Microbiological & Chemical Food Analysis dairy-products food proteins identification ms quantitative proteomic analysis RIKILT - Business Unit Veiligheid & Gezondheid PRI Bioscience BU Microbiologische & Chemische Voedselanalyse Journal of Separation Science 32 8 1216 1223 16159306 urn:nbn:nl:ui:32-379988/obj 2026-03-31 http://purl.org/eprint/accessRights/RestrictedAccess