2026-06-09T20:23:10+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/392362 2026-03-31

urn:nbn:nl:ui:32-392362 2026-03-31 urn:nbn:nl:ui:32-392362/mods A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis Wang D. aut Griffitts J. aut Starker C. aut Fedorova E. 314606157 0000-0003-3953-1282 0000000390404924 aut Limpens E.H.M. 266277233 0000-0002-9668-4085 0000000396793633 aut Ivanov S.E. 314628193 0000000392589432 aut Bisseling T. 068470290 0000-0001-5494-8786 0000000038008658 aut Long S. aut text info:eu-repo/semantics/article 2010 10.1126/science.1184096 2-s2.0-77649212137 000274901100035 en The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1 EPS-1 Molecular Biology (MOB) define endoplasmic-reticulum fixation gene-expression medicago-truncatula membrane mutants plant root-nodules signal peptidase activity EPS-1 Molecular Biology (MOB) Science 327 5969 1126 1129 00368075 urn:nbn:nl:ui:32-392362/obj 2026-03-31 http://purl.org/eprint/accessRights/RestrictedAccess