2026-06-10T02:25:39+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/405639 2026-03-31 edurep openaire public

urn:nbn:nl:ui:32-405639 2026-03-31 urn:nbn:nl:ui:32-405639/mods Development and validation of real-time PCR screening methods for detection of cry1a.105 and cry2ab2 genes in genetically modified organisms Dinon A.Z. aut Prins T.W. 314507485 0000000389558504 aut van Dijk J.P. 252654056 0000-0001-7731-2457 0000000392689126 aut Arisi C.M. aut Scholtens-Toma I.M.J. 314602135 0000000392212993 aut Kok E.J. 11529449X 0000-0003-1472-6710 0000000394169028 aut text info:eu-repo/semantics/article 2011 10.1007/s00216-011-4875-9 2-s2.0-79955565637 000289792400023 en Primers and probes were developed for the element-specific detection of cry1A.105 and cry2Ab2 genes, based on their DNA sequence as present in GM maize MON89034. Cry genes are present in many genetically modified (GM) plants and they are important targets for developing GMO element-specific detection methods. Element-specific methods can be of use to screen for the presence of GMOs in food and feed supply chains. Moreover, a combination of GMO elements may indicate the potential presence of unapproved GMOs (UGMs). Primer-probe combinations were evaluated in terms of specificity, efficiency and limit of detection. Except for specificity, the complete experiment was performed in 9 PCR runs, on 9 different days and by testing 8 DNA concentrations. The results showed a high specificity and efficiency for cry1A.105 and cry2Ab2 detection. The limit of detection was between 0.05 and 0.01 ng DNA per PCR reaction for both assays. These data confirm the applicability of these new primer-probe combinations for element detection that can contribute to the screening for GM and UGM crops in food and feed samples. RIKILT - Business Unit Safety & Health Rikilt B&T Novel Foods en Agroketens bacillus-thuringiensis crystal proteins fed diets glyphosate-tolerant gmos identification performance reference corn RIKILT - Business Unit Veiligheid & Gezondheid Rikilt B&T Novel Foods en Agroketens Analytical and Bioanalytical Chemistry 400 5 1433 1442 16182642 urn:nbn:nl:ui:32-405639/obj 2026-03-31 http://purl.org/eprint/accessRights/OpenAccess