2026-06-10T01:03:25+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/407301 2026-03-31

urn:nbn:nl:ui:32-407301 2026-03-31 urn:nbn:nl:ui:32-407301/mods Comparison of two GM maize varieties with a near-isogenic non-GM variety using transcriptomics, proteomics and metabolomics Barros E. aut Lezar S. aut Anttonen M.J. aut van Dijk J.P. 252654056 0000-0001-7731-2457 0000000392689126 aut Rohlig R.M. aut Kok E.J. 11529449X 0000-0003-1472-6710 0000000394169028 aut Engel K.H. aut text info:eu-repo/semantics/article 2010 10.1111/j.1467-7652.2009.00487.x 2-s2.0-77955390164 000276407700003 en P>The aim of this study was to evaluate the use of four nontargeted analytical methodologies in the detection of unintended effects that could be derived during genetic manipulation of crops. Three profiling technologies were used to compare the transcriptome, proteome and metabolome of two transgenic maize lines with the respective control line. By comparing the profiles of the two transgenic lines grown in the same location over three growing seasons, we could determine the extent of environmental variation, while the comparison with the control maize line allowed the investigation of effects caused by a difference in genotype. The effect of growing conditions as an additional environmental effect was also evaluated by comparing the Bt-maize line with the control line from plants grown in three different locations in one growing season. The environment was shown to play an important effect in the protein, gene expression and metabolite levels of the maize samples tested where 5 proteins, 65 genes and 15 metabolites were found to be differentially expressed. A distinct separation between the three growing seasons was also found for all the samples grown in one location. Together, these environmental factors caused more variation in the different transcript/protein/metabolite profiles than the different genotypes. Rikilt B&T Novel Foods en Agroketens food gene-expression h-1-nmr spectroscopy hybridization microarrays mutants nmr plants safety assessment tool Rikilt B&T Novel Foods en Agroketens Plant Biotechnology Journal 8 4 436 451 14677644 urn:nbn:nl:ui:32-407301/obj 2026-03-31 http://purl.org/eprint/accessRights/RestrictedAccess