2026-06-10T01:03:28+02:00 https://library.wur.nl/oai

oai:library.wur.nl:wurpubs/416332 2026-03-31

urn:nbn:nl:ui:32-416332 2026-03-31 urn:nbn:nl:ui:32-416332/mods Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions Santos A.M. 408472251 aut Janssen M.G.J. 238521214 0000-0002-5754-6050 0000000394183233 aut Lamers P.P. 314626751 0000000392699949 aut Evers W.A.C. 386017239 0000000493348689 aut Wijffels R.H. 124254241 0000-0001-7630-4295 0000000023679774 aut text info:eu-repo/semantics/article 2012 10.1016/j.biortech.2011.10.084 2-s2.0-84655167176 000301155800080 en The effect of elevated pH and salt concentration on the growth of the freshwater microalga Neochloris oleoabundans was investigated. A study was conducted in 24-well plates on the design of a growth medium and subsequently applied in a photobioreactor. An artificial seawater medium with reduced Ca(2+) and PO(4)(3-) could prevent mineral precipitation at high pH levels. Growth was characterized in this new medium at pH8.1 and at pH10.0, with 420mM of total salts. Specific growth rates of 0.08h(-1) at pH8.1 and 0.04h(-1) at pH10.0 were obtained under controlled turbidostat cultivation. The effect of nitrogen starvation on lipid accumulation was also investigated. Fatty acids content increased not only with nitrogen limitation but also with a pH increase (up to 35% in the dry biomass). Fluorescence microscopy gave visual proof that N. oleoabundans accumulates oil bodies when growing in saline conditions at high pH. Bio Process Engineering VLAG biodiesel biofuels biomass carbon-dioxide economics feedstocks flue-gas metabolism photobioreactor Bio Process Engineering VLAG Bioresource Technology 104 593 599 09608524 urn:nbn:nl:ui:32-416332/obj 2026-03-31 http://purl.org/eprint/accessRights/RestrictedAccess