2013-05-18T08:49:42Zhttp://library.wur.nl/oai

oai:library.wur.nl:wurpubs/351322 2011-03-02

urn:nbn:nl:ui:32-351322 2011-03-02 urn:nbn:nl:ui:32-351322/mods A comparative study on the effect of algal and fish oil on viability and cell proliferation of Caco-2 cells Beelen, van V.A. aut Roeleveld J. aut Mooibroek H. aut Sijtsma L. aut Bino R.J. aut Bosch H.J. aut Rietjens I.M.C.M. aut Alink G.M. aut 070543097 075055570 071872353 073731374 072408057 067707130 text info:eu-repo/semantics/article 2007 en Polyunsaturated fatty acid (PUFA) rich micro-algal oil was tested in vitro and compared with fish oil for antiproliferative properties on cancer cells in vitro. Oils derived from Crypthecodinium cohnii, Schizochytrium sp. and Nitzschia laevis, three commercial algal oil capsules, and menhaden fish oil were used in cell viability and proliferation tests with human colon adenocarcinoma Caco-2 cells. With these tests no difference was found between algal oil and fish oil. The nonhydrolysed algal oils and fish oil showed a much lower toxic effect on cell viability, and cell proliferation in Caco-2 cells than the hydrolysed oils and the free fatty acids (FFAs). Eicosapentaenoic acid (EPA; C20:5n ¿ 3) and docosahexaenoic acid (DHA; C22:6n ¿ 3) were used as samples for comparison with the tested hydrolysed and nonhydrolysed oils. The hydrolysed samples showed comparative toxicity as the free fatty acids and no difference between algal and fish oil. Oxidative stress was shown to play a role in the antiproliferative properties of EPA and DHA, as ¿-tocopherol could partially reverse the EPA/DHA-induced effects. The results of the present study support a similar mode of action of algal oil and fish oil on cancer cells in vitro, in spite of their different PUFA content Biobased Products Laboratorium voor Plantenfysiologie Bioscience Sectie Toxicologie Food and Chemical Toxicology 45 5 716 724 URN:ISSN:0278-6915 urn:nbn:nl:ui:32-351322/obj 2010-01-14 qhttp://purl.org/eprint/accessRights/RestrictedAccess