2024-03-29T11:18:18+01:00
https://library.wur.nl/oai
oai:library.wur.nl:wurpubs/405271
2024-03-07
urn:nbn:nl:ui:32-405271
2024-03-07
urn:nbn:nl:ui:32-405271/mods
Bioreactor and substrate selection for solid-state cultivation of the malaria mosquito control agent Metarhizium anisopliae
van Breukelen
F.R.
31459793X
0000000392973565
aut
Haemers
S.
314621903
0000000392512777
aut
Wijffels
R.H.
124254241
0000-0001-7630-4295
0000000023679774
aut
Rinzema
A.
073303542
0000-0002-2702-5721
000000039313670X
aut
text
info:eu-repo/semantics/article
2011
10.1016/j.procbio.2010.11.023
79951508518
000288419900021
en
Metarhizium anisopliae spores are a promising alternative to chemical insecticides against malaria mosquitoes. In-house application resulting in infection of mosquitoes with the fungus can strongly contribute to reducing malaria transmission. For such application, fungal spores need to be produced in large quantities. Cultivation of the fungus on several solid substrates and in two bioreactor types for solid state fermentation was studied. Our experiments showed that M. anisopliae cannot withstand mixing; therefore aerated static packed beds are the most suitable reactor type. Based on spore yields (0.18 kg spores kg-1 substrate, 5.5 × 1014 spores m-3 reactor volume) and its favorable physical properties, hemp impregnated with a nutrient medium was selected for further research. Total nutrient concentrations between 250 and 313 kg m-3 medium gave the highest productivities; the maximum concentration imposed by water losses for evaporative cooling was estimated at 240 kg m-3. Combined autoclaving of nitrogen and carbon sources consistently gave lower average productivities. Pilot-scale fermentation demonstrated the feasibility of scale-up of the aerated packed bed with impregnated hemp, showing its potential for large scale production of M. anisopliae spores
Bio Process Engineering
VLAG
beauveria-bassiana
conidia
fermentation
fungi
growth
model
products
scale-up
sporulation
temperature
Bio Process Engineering
VLAG
Process Biochemistry
46
3
751
757
13595113
urn:nbn:nl:ui:32-405271/obj
2024-03-07
http://purl.org/eprint/accessRights/RestrictedAccess