2024-03-29T02:29:04+01:00
https://library.wur.nl/oai
oai:library.wur.nl:wurpubs/406626
2024-03-11
urn:nbn:nl:ui:32-406626
2024-03-11
urn:nbn:nl:ui:32-406626/mods
Synthesis of Peptide Amides using Sol-Gel Immobilized Alcalase in Batch and Continuous Reaction System
Corici
L.N.
aut
Frissen
A.E.
078252679
0000000396804187
aut
van Zoelen
D.J.
aut
Eggen
I.F.
aut
Peter
F.
aut
Davidescu
C.
aut
Boeriu
C.G.
314600051
0000-0003-4903-6621
0000000393659092
aut
text
info:eu-repo/semantics/article
2011
79955715090
en
Two commercial proteases from Bacillus licheniformis (Alcalase 2.4 L FG and Alcalase 2.5 L, Type DX) were screened for the production of Z-Ala-Phe-NH2 in batch reaction. Alcalase 2.4 L FG was the most efficient enzyme for the C-terminal amidation of Z-Ala-Phe-OMe using ammonium carbamate as ammonium source. Immobilization of protease has been achieved by the sol-gel method, using dimethyldimethoxysilane (DMDMOS) and tetramethoxysilane (TMOS) as precursors (unpublished results). In batch production, about 95% of Z-Ala-Phe-NH2 was obtained at 30°C after 24 hours of incubation. Reproducibility of different batches of commercial Alcalase 2.4 L FG preparations was also investigated by evaluating the amidation activity and the entrapment yields in the case of immobilization. A packed-bed reactor (0.68 cm ID, 15.0 cm long) was operated successfully for the continuous synthesis of peptide amides. The immobilized enzyme retained the initial activity over 10 cycles of repeated use in continuous reactor at ambient temperature. At 0.75 mL/min flow rate of the substrate mixture, the total conversion of Z-Ala-Phe-OMe was achieved after 5 hours of substrate recycling. The product contained about 90% peptide amide and 10% hydrolysis byproduct.
BBP Sustainable Chemistry & Technology
Packed-bed reactor
Peptide amide
Protease
Sol-gel immobilization
BBP Sustainable Chemistry & Technology
World Academy of Science, Engineering and Technology
5
4
794
799
20703740
urn:nbn:nl:ui:32-406626/obj
2024-03-11
http://purl.org/eprint/accessRights/RestrictedAccess