2024-03-28T23:10:13+01:00
https://library.wur.nl/oai
oai:library.wur.nl:wurpubs/417240
2024-03-07
urn:nbn:nl:ui:32-417240
2024-03-07
urn:nbn:nl:ui:32-417240/mods
Characterization of Translocation of Silver Nanoparticles and Effects on Whole-Genome Gene Expression Using an In Vitro Intestinal Epithelium Coculture Model
Bouwmeester
H.
240158806
0000-0001-9318-4272
000000039712700X
aut
Poortman
J.H.
314617876
0000000393771615
aut
Peters
R.J.B.
075101912
0000-0002-3948-5012
0000000396746263
aut
Wijma
E.
314608060
0000000394383008
aut
Kramer
E.H.M.
314616128
aut
Makama
S.
aut
Puspitaninganindita
K.
aut
Marvin
H.J.P.
074306405
0000000393772482
aut
Peijnenburg
A.A.C.M.
073901180
0000000389959535
aut
Hendriksen
P.J.M.
151603545
0000-0002-0951-3270
0000000393723701
aut
text
info:eu-repo/semantics/article
2011
10.1021/nn2007145
80051532158
000290826800081
en
Applications of nanoparticles in the food sector are eminent. Silver nanoparticles are among the most frequently used, making consumer exposure to silver nanoparticles inevitable. Information about uptake through the intestines and possible toxic effects of silver nanoparticles is therefore very important but still lacking. In the present study, we used an in vitro model for the human intestinal epithelium consisting of Caco-2 and M-cells to study the passage of silver nanoparticles and their ionic equivalents and to assess their effects on whole-genome mRNA expression. This in vitro intestine model was exposed to four sizes of silver nanoparticles for 4 h. Exposure to silver ions was included as a control since 6-17% of the silver nanoparticles were found to be dissociated into silver ions. The amount of silver ions that passed the Caco-2 cell barrier was equal for the silver ion and nanoparticle exposures. The nanoparticles induced clear changes in gene expression in a range of stress responses including oxidative stress, endoplasmatic stress response, and apoptosis. The gene expression response to silver nanoparticles, however, was very similar to that of AgNO(3). Therefore, the observed effects of the silver nanoparticles are likely exerted by the silver ions that are released from the nanoparticles.
RIKILT - R&C Contaminanten
Rikilt B&T Novel Foods en Agroketens
Rikilt B&T Toxicologie en Effectanalyse
caco-2 cells
cytotoxicity
m-cells
mechanism
nano-silver
nanotechnologies
release
stress
toxicity
transport
RIKILT - R&C Contaminanten
Rikilt B&T Novel Foods en Agroketens
Rikilt B&T Toxicologie en Effectanalyse
ACS Nano
5
5
13
4091
4103
19360851
urn:nbn:nl:ui:32-417240/obj
2024-03-07
http://purl.org/eprint/accessRights/RestrictedAccess