2024-03-28T23:10:13+01:00 https://library.wur.nl/oai
oai:library.wur.nl:wurpubs/417240 2024-03-07
urn:nbn:nl:ui:32-417240 2024-03-07 urn:nbn:nl:ui:32-417240/mods Characterization of Translocation of Silver Nanoparticles and Effects on Whole-Genome Gene Expression Using an In Vitro Intestinal Epithelium Coculture Model Bouwmeester H. 240158806 0000-0001-9318-4272 000000039712700X aut Poortman J.H. 314617876 0000000393771615 aut Peters R.J.B. 075101912 0000-0002-3948-5012 0000000396746263 aut Wijma E. 314608060 0000000394383008 aut Kramer E.H.M. 314616128 aut Makama S. aut Puspitaninganindita K. aut Marvin H.J.P. 074306405 0000000393772482 aut Peijnenburg A.A.C.M. 073901180 0000000389959535 aut Hendriksen P.J.M. 151603545 0000-0002-0951-3270 0000000393723701 aut text info:eu-repo/semantics/article 2011 10.1021/nn2007145 80051532158 000290826800081 en Applications of nanoparticles in the food sector are eminent. Silver nanoparticles are among the most frequently used, making consumer exposure to silver nanoparticles inevitable. Information about uptake through the intestines and possible toxic effects of silver nanoparticles is therefore very important but still lacking. In the present study, we used an in vitro model for the human intestinal epithelium consisting of Caco-2 and M-cells to study the passage of silver nanoparticles and their ionic equivalents and to assess their effects on whole-genome mRNA expression. This in vitro intestine model was exposed to four sizes of silver nanoparticles for 4 h. Exposure to silver ions was included as a control since 6-17% of the silver nanoparticles were found to be dissociated into silver ions. The amount of silver ions that passed the Caco-2 cell barrier was equal for the silver ion and nanoparticle exposures. The nanoparticles induced clear changes in gene expression in a range of stress responses including oxidative stress, endoplasmatic stress response, and apoptosis. The gene expression response to silver nanoparticles, however, was very similar to that of AgNO(3). Therefore, the observed effects of the silver nanoparticles are likely exerted by the silver ions that are released from the nanoparticles. RIKILT - R&C Contaminanten Rikilt B&T Novel Foods en Agroketens Rikilt B&T Toxicologie en Effectanalyse caco-2 cells cytotoxicity m-cells mechanism nano-silver nanotechnologies release stress toxicity transport RIKILT - R&C Contaminanten Rikilt B&T Novel Foods en Agroketens Rikilt B&T Toxicologie en Effectanalyse ACS Nano 5 5 13 4091 4103 19360851 urn:nbn:nl:ui:32-417240/obj 2024-03-07 http://purl.org/eprint/accessRights/RestrictedAccess