Record nummer 2070639
Titel Validation report of a real-time PCR assay for the identification of Clavibacter michiganensis subsp. michiganensis (Cmm) isolates
toon extra info.
Adriaan Vermunt and Floor den Otter
Auteur(s) Vermunt, A. ; Otter, F. den
Uitgever [Delfgauw] : Groen Agro Control
Jaar van uitgave 2010
Pagina's 1 online resource (PDF, 14 pages) illustrations
Online full text
Trefwoorden (cab) clavibacter michiganensis subsp. michiganensis / real time pcr / polymerase-kettingreactie / tomaten / solanum lycopersicum / plantenziektebestrijding / moleculaire detectie / plantenziekten
Rubrieken Landbouwkundige bacteriologie (algemeen) / Biologische technieken
Publicatie type Boek
Taal Engels
Toelichting (Engels) This reports presents complete validation data related to recently developed primer-probe combinations for the identification of Clavibacter michiganensis subsp. michiganensis (Cmm) isolates with the use of a real-time PCR assay. The primers and probes were developed on two loci: on the intergenic spacer between the 16S and 23S ribosomal RNA genes (IGS) and on the pathogenity-1 gene (Pat-1), which is located on the plasmid pCM2. The validated method is qualitative and consists of a DNA extraction from pure colony material followed by a duplex real-time PCR assay based on the primer-probe combinations for IGS and Pat-1. The method is very specific, because only the 20 tested pathogenic Cmm isolates were positive for both primer-probe combinations. Negative results were obtained from 12 Cmm look-alikes, 4 Cm isolates and 9 other tomato pathogens. Tests on repeatability, reproducibility and robustness of this assay performed well.
Reacties
Er zijn nog geen reacties. U kunt de eerste schrijven!
Schrijf een reactie

To support researchers to publish their research Open Access, deals have been negotiated with various publishers. Depending on the deal, a discount is provided for the author on the Article Processing Charges that need to be paid by the author to publish an article Open Access. A discount of 100% means that (after approval) the author does not have to pay Article Processing Charges.

For the approval of an Open Access deal for an article, the corresponding author of this article must be affiliated with Wageningen University & Research.