Record nummer 1935829
Titel artikel Experimental evaluation of decrease in bacterial activity due to cell death and activity decay in activated sludge
Auteur(s) Hao, X. ; Wang, Q. ; Zhang, X. ; Cao, Y. ; Mark Loosdrecht, C.M.v
Tijdschrifttitel Water research : the journal of the International Association on Water Quality
Deel(Jaar)Nummer 43(2009)14
Paginering 3604 - 3612
Online full text
Trefwoorden (cab) geactiveerd slib / biologische achteruitgang / microbiële activiteiten / bacteriën / ammonium / nitriet / kwantitatieve analyse / heterotrofe micro-organismen / zuurstofconsumptie / afvalwaterbehandeling / slibzuivering
Rubrieken Afvalwaterbehandeling
Publicatie type Artikel
Taal Engels
Toelichting (Engels) Decrease in bacterial activity (cell decay) in activated sludge can be attributed to cell death (reduction in the amount of active bacteria) and activity decay (reduction in the specific activity of active bacteria). The aim of this experimental study was to quantitatively evaluate the contribution of cell death and activity decay to microbial activity, for both nitrifying bacteria (ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB)) and ordinary heterotrophic organisms (OHOs). Decay rates were determined by changes in maximal oxygen uptake rates (OURs) after decay under famine conditions; live/dead staining was used to identify the ratio of live cells to total cells by evaluating the integrity of the cell membrane; fluorescence in-situ hybridization (FISH) (targeted at viable cells) was employed to determine the ratios of AOB and NOB to total viable bacteria. The contribution of cell death and activity decay to microbial activity could be thereafter evaluated.
Betrokken instanties Technische Universiteit Delft, Vakgroep Bioprocestechnologie
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