Login

Hydrotheek

Record nummer 2047244
Titel artikel Monitoring microbiological changes in drinking water systems using a fast and reproducible flow cytometric method
Auteur(s) Prest, E.I. ; Hammes, F. ; Kötzsch, S. ; Loosdrecht, M.C.M. van ; Vrouwenvelder, J.S.
Tijdschrifttitel Water research : the journal of the International Association on Water Quality
Deel(Jaar)Nummer 47(2013)19
Paginering 7131 - 7142
Online full text
Op papier Haal het document, vind aanverwante informatie of gebruik andere SFX-diensten
Trefwoorden (cab) watervoorziening / drinkwater / waterkwaliteit / bacteriologie / doorstroomcytometrie / stabiliteit / monitoring / distributie / fluorescentie
Rubrieken Watervoorziening
Publicatie type Artikel
Taal Engels
Toelichting (Engels) Flow cytometry (FCM) is a rapid, cultivation-independent tool to assess and evaluate bacteriological quality and biological stability of water. Here we demonstrate that a stringent, reproducible staining protocol combined with fixed FCM operational and gating settings is essential for reliable quantification of bacteria and detection of changes in aquatic bacterial communities. Triplicate measurements of diverse water samples with this protocol typically showed relative standard deviation values and 95% confidence interval values below 2.5% on all the main FCM parameters. We propose a straightforward and instrument-independent method for the characterization of water samples based on the combination of bacterial cell concentration and fluorescence distribution. Analysis of the fluorescence distribution (or so-called fluorescence fingerprint) was accomplished firstly through a direct comparison of the raw FCM data and subsequently simplified by quantifying the percentage of large and brightly fluorescent high nucleic acid (HNA) content bacteria in each sample.
Betrokken instanties Technische Universiteit Delft, Vakgroep Bioprocestechnologie
Wetsus
Reacties
Er zijn nog geen reacties. U kunt de eerste schrijven!
Schrijf een reactie

To support researchers to publish their research Open Access, deals have been negotiated with various publishers. Depending on the deal, a discount is provided for the author on the Article Processing Charges that need to be paid by the author to publish an article Open Access. A discount of 100% means that (after approval) the author does not have to pay Article Processing Charges.

For the approval of an Open Access deal for an article, the corresponding author of this article must be affiliated with Wageningen University & Research.