Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 109209
Title Optimization of Production and Downstream Processing of the Almond ß-Glucosidase-Mediated Glucosylation of Glycerol
Author(s) Roode, B.M. de; Peters, S.W.P.G.; Franssen, M.C.R.; Padt, A. van der; Groot, Æ. de; Boom, R.M.
Source Biotechnology and Bioengineering 72 (2001)5. - ISSN 0006-3592 - p. 568 - 572.
Department(s) Organic Chemistry
Sub-department of Food and Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract This article describes the synthesis of glyceryl glucoside from glycerol and glucose with almond β-glucosidase as the catalyst. A yield of 54ø0.45 mmol/g) was obtained. The influence of the enzyme stability, the water concentration, and the water activity on the glucoside yield were determined. A molar fraction-based equilibrium constant of 2.4 ± 0.6 was found, with which the glucoside yield could be calculated for all possible combinations of initial substrate and water fractions in the reaction mixture. A model was used to optimize the glucoside yield while minimizing one of the substrate concentrations at equilibrium. This straightforward model gives a good prediction of the measured glucoside yield, according to a parity plot.
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