Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 109305
Title Regulation of interleukin 1 beta RNA expression in the common carp, Cyprinus carpio L
Author(s) Engelsma, M.Y.; Stet, R.J.M.; Schipper, H.; Verburg-van Kemenade, B.M.L.
Source Developmental and Comparative Immunology 25 (2001). - ISSN 0145-305X - p. 195 - 203.
Department(s) Cell Biology and Immunology
Experimental Zoology
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract The intron-exon organisation of the carp IL-1 gene consists of 2455 bp and comprises seven exons. Three IL-1 RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1–7 with introns 5 and 6; and (3) exon 1–7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1 mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1 mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1 producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1 intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-B pathway in carp IL-1 expression was shown with suppression of the LPS-induced IL-1 expression by NF-B inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1 transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription.
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