Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 109556
Title Population and molecular genetics of root-knot nematodes
Author(s) Dautova, M.
Source Wageningen University. Promotor(en): J. Bakker; F.J. Gommers; G. Smant. - S.l. : S.n. - ISBN 9789058084286 - 126
Department(s) Laboratory of Nematology
EPS
Publication type Dissertation, internally prepared
Publication year 2001
Keyword(s) meloidogyne - plantenparasitaire nematoden - populatiegenetica - moleculaire genetica - meloidogyne - plant parasitic nematodes - population genetics - molecular genetics
Categories Plant Parasitic Nematodes
Abstract

This thesis describes studies of root-knot nematodes Meloidogyne spp. - an economically important pest in agriculture - using population and molecular genetics. Variability in virulence to Mi bearing tomato genotypes is shown for Meloidogyne spp . isolates and their impact of implementation of proper management systems is discussed. Genetic polymorphisms in Meloidogyne spp. were assessed using molecular techniques that reveal nuclear and mitochondrial DNA divergence on different hierarchical levels. cDNA library constructed from the pre-parasitic second stage juveniles of Meloidogyne incognita was screened by single pass 5'-end sequencing to investigate genes expressed at the onset of parasitism. One expressed sequence tag homologous to an endoxylanase was used to identify a novel cell wall degrading enzyme - xylanase. Several lines of evidence support the endogenous origin of the nematode xylanase. The amino acid sequence revealed a high similarity with bacterial xylanases indicating that nematode xylanase may have been acquired from bacteria by horizontal gene transfer.

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